Classifications

• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
  • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
  • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
  • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L13/00—Cleaning or rinsing apparatus
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/34—Organic compounds containing sulfur
  • C11D3/349—Organic compounds containing sulfur additionally containing nitrogen atoms, e.g. nitro, nitroso, amino, imino, nitrilo, nitrile groups containing compounds or their derivatives or thio urea
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/38—Products with no well-defined composition, e.g. natural products
  • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
  • C11D3/38618—Protease or amylase in liquid compositions only
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
  • G01N35/1004—Cleaning sample transfer devices
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
  • C11D2111/10—Objects to be cleaned
  • C11D2111/14—Hard surfaces

Definitions

• the present invention relates to a cleaner composition and method of use for cleaning automated blood analyzers, more particularly relates to a cleaner composition that is substantially free of formaldehyde content.
• An effective cleaning reagent and cleaning procedures are necessary for automated and semi-automated blood analyzers for cleaning the instrument components that are in contact with the blood samples, or the mixtures of a blood sample and the reagents used for blood analysis, and maintaining the analyzers in proper operating conditions.
• the whole blood sample will be treated with various reagents on the instrument prior to the measurement.
• an aliquot of a whole blood sample is diluted by an isotonic diluent to form a sample mixture for the red blood cell and platelet measurements.
• Another aliquot of the whole blood sample is mixed with a lytic reagent to form a second sample mixture for the white blood cell and hemoglobin measurements, wherein the red blood cells are lysed.
• sample mixtures of the blood sample and the reagents contain proteins, and debris from the lysed cellular membranes. These materials can precipitate on the surface of certain instrument components which are in contact with the sample mixtures during the analysis of the blood sample. Furthermore, these materials can also form insoluble complexes with certain chemicals in the reagent and precipitate on the surface of the instrument components. Without an effective cleaning on a regular basis, the precipitates can build up around the apertures and flow cell, which are used for blood cell counting, and along the wall of tubings that are in contact with the sample mixtures.
• Enzymatic cleaning reagents are known in the art, and they are effective in breaking down the proteins and blood debris by enzymatic reactions. Surfactants are commonly used in the cleaning reagents for dissolving or dispersing the debris and large organic molecules.
• Antimicrobials are commonly used in the cleaning reagents as preservatives to inhibit the growth of microorganisms, such as bacteria, fungi, and yeast, and to ensure shelf-life, quality and performance of the reagent products. Furthermore, it is also important to prevent microorganism growth inside the instruments, such as inside the reagent tubings and other parts of the fluid feeding system, because organism growth can cause contamination of the instrument components, and can further interfere with system performance. For example, the organisms can be counted as blood cells, or cause false flaggings which indicate the presence of abnormal blood types in a blood sample.
• the present invention is directed to a formaldehyde-free cleaner composition, which comprises one or more surfactant and one or more non-formaldehyde releasing antimicrobial in an aqueous solution.
• the cleaner composition further comprises a proteolytic enzyme, an alkaline metal salt, and a buffer to maintain the pH of the cleaner composition in a range from about 7 to about 12, preferably from about 8 to about 10.
• the cleaner composition has a formaldehyde concentration equal or less than 1 ppm.
• an isothiazolone compound is used as the antimicrobial, more preferably, two or more isothiazolone compounds are used in the cleaner composition to preserve the composition and to support maintaining the blood analyzer.
• the surfactant is a nonionic surfactant, an anionic surfactant, or their combinations, and the proteolytic enzyme is a subtilisin.
• the present invention is directed to a method of cleaning a blood analyzer, which comprises the steps of providing a formaldehyde-free cleaner composition to a blood analyzer; delivering the cleaner composition to the parts of the blood analyzer, which are in contact with a sample mixture formed by a blood sample and one or more reagent during an analysis of the blood sample; contacting the parts with the cleaner composition for a period of time; and rinsing the cleaner composition out from the blood analyzer.
• the blood analyzers include hematology analyzers, flow cytometers, or clinical chemistry analyzers.
• the present invention provides a formaldehyde- free aqueous cleaner composition, which comprises one or more surfactant and one or more non-formaldehyde releasing antimicrobial.
• the cleaner composition further comprises a proteolytic enzyme; one or more alkaline metal salt; and a buffer to maintain a pH of the cleaner composition in a range from about 7 to about 12.
• the cleaner composition is substantially free of formaldehyde.
• the cleaner composition has a formaldehyde concentration equal or less than 1 part per million (ppm).
• substantially free of formaldehyde used herein means that the formaldehyde concentration of an aqueous composition meets the formaldehyde-free definition of the Environmental Protection Agency (EPA), which specifies that as assayed by a defined HPLC analysis method (EPA Method 8315A), the formaldehyde concentration of an aqueous composition is equal or less than 1 ppm.
• EPA Environmental Protection Agency
• HPLC analysis method EPA Method 8315A
• Surfactants suitable for the cleaner composition of the present invention include nonionic surfactant, anionic surfactant, or combinations thereof.
• Suitable examples of nonionic surfactant include, but are not limited to, ethoxylated alkyl phenol, ethoxylated alkyl alcohol, or combinations thereof.
• the ethoxylated alkyl alcohol has a linear alkyl group having 8 to 18 carbon atoms and 10 to 40 moles of ethylene oxide; and the ethoxylated alkyl phenol can have either a linear alkyl group or branched alkyl groups having 6 to 10 carbon atoms and 4 to 30 moles of ethylene oxide.
• a combination of two ethoxylated nonyl phenols is used.
• anionic surfactant include, but are not limited to, ethoxylated alkyl phenol phosphate ester, ethoxylated alkyl phosphate ester, and ethoxylated alkyl sulfate ester.
• the ethoxylated alkyl phenol phosphate ester has a linear alkyl group having 8 to 10 carbon atoms, and 4 to 30 moles of ethylene oxide.
• a combination of an ethoxylated nonyl phenol and an ethoxylated alkyl phenol phosphate ester is used.
• the concentration of the surfactant(s) in the cleaner composition can be in a range from about 1 g/l to about 30 g/l.
• protease is any enzyme that catalyzes the splitting of proteins into smaller peptide fractions and amino acids by a process known as proteolysis.
• proteolytic enzymes of various qualities and origins and having activity in various pH ranges of from 7-12 are available and can be used in the present invention.
• suitable proteolytic enzymes are the subtil isins which are obtained from particular strains of B. subtilis, B. licheniformis, such as the commercially available subtilisins MaxataseTM, as supplied by Genencor International N. V., Delft, Holland, and AlcalaseTM as supplied by Novozymes lndustri A/S, Copenhagen, Denmark.
• protease obtained from a strain of Bacillus having maximum activity throughout the pH range of 8-12, being commercially available from Novozymes lndustri A/S under the tradenames Esperase ® and Savinase ® .
• the preparation of these and analogous enzymes is described in GB 1 243 785.
• Other commercial available proteases are KazusaseTM (from Showa- Denko of Japan), OptimaseTM (from Miles Kali-Chemie, Hannover, West Germany), and SuperaseTM (from Pfizer of the United States).
• the concentration of the proteolytic enzyme in the cleaner composition can be in a range from about 0.15 ⁇ g/ml to about 500 ⁇ g/ml. It is noted that the concentration described herein is expressed by the amount of pure enzyme in the cleaner composition.
• alkaline metal salts are effective in stabilizing the proteolytic enzyme in the cleaner composition of the present invention.
• alkaline metal formate, or alkaline metal halide can be used. More preferably, a combination of alkaline metal formate and alkaline metal halide is used. In an exemplary embodiment shown in Example 1 , sodium formate and sodium chloride are used.
• concentration of the alkaline metal salt or salts in the cleaner composition can be in a range from about 1 g/l to about 16 g/l.
• Suitable buffers include, but are not limited to, sodium carbonate, sodium bicarbonate, boric acid, glycylglycine, glycine, tris hydroxymethylaminoethane (TRIS), N-ths(hydroxymethyl)methyl-2-aminoethane sulfonic acid (TES), N-2-hydroxyehtylpiperazine-N'-2-ethanesulfonic acid (HEPES), N-2-hydroxyethylpiperazine-N'-3-propane sulfonic acid (HEPPS), 2-(N- cyclohexylamino) ethane sulfonic acid (CHES), piperazine-1 ,4-bis(2-ethane sulfonic acid) (PIPES), 3-((tris(hydroxymethyl)methyl)amino)propanesulfonic acid (TAPS), or combinations thereof.
• TMS tris hydroxymethylaminoethane
• TES N-ths(hydroxymethyl)methyl-2
• formaldehyde-releasing agent used herein refers to any chemical which contains or releases, upon chemical reaction, formaldehyde molecule.
• non- formaldehyde releasing antimicrobial used herein refers to a chemical which has antimicrobial function, but does not contain, or release formaldehyde during storage of a reagent product which contains the antimicrobial.
• non-formaldehyde releasing antimicrobials can be used for the purpose of the present invention.
• isothiazolone compounds include, but are not limited to, isothiazolone compounds, phenoxyethanol, 2(2- phenoxyethoxy) methyldibromoglutaronitrile, amine abduct, 2,2-dibromo-3- nithloprionamide, or combinations thereof. Particularly suitable are isothiazolone compounds. Suitable examples of isothiazolone compounds include 5-chloro-2- methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, 1 ,2 benzisothiazolin-3- one, and derivatives thereof.
• derivatives refers to homologs which are the compounds differing regularly by the successive addition of the same chemical group, e.g., by -CH 2 - groups, or differing by changing one with another among halide functional group.
• Proclin ® 150 which contains a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4- isothiazolin-3-one, can be obtained from Rohm and Haas Company, Philadelphia, PA. 1 ,2 benzisothiazolin-3-one is commercially available under the tradenames Mergal ® K10N from Troy Corporation, Florham Park, New Jersey, and Acticide ® B10 from Thor gmbh Speyer, Germany.
• two or more non-formaldehyde releasing antimicrobials are used together to achieve a synergetic effect in inhibiting the growth of a broad spectrum of organisms including bacteria, fungi, and yeast, particularly those frequently observed in the automated blood analyzer systems.
• a combination of 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4- isothiazolin-3-one, and 1 , 2 benzisothiazolin-3-one is used in the cleaner composition, as shown in Example 1 hereinafter.
• the concentration of the antimicrobial(s) in the cleaner composition can be in a range from about 0.03 g/l to about 0.8 g/l.
• Yeast and molds No increase from the initial calculated count at 7, 14, and 28 days.
• Example 2 illustrates preservative effectiveness tests of two cleaner compositions, Formula A and Formula B of Example 1.
• Table 2 the inoculation levels of all organisms tested were reduced from 10 5 to below 10 2 (CFU/ml) in Formula A as early as 48 hours, and the inoculation levels were maintained below 10 2 (CFU/ml) during the tests at 7, 14 and 28 days.
• the antimicrobials in Formula B were effective in eliminating all organisms tested in a 48 hour period of time, which was substantially shorter than 7 days as required by USP 26.
• the concentration of formaldehyde is analyzed using high performance liquid chromatography (HPLC) according to EPA Method 8315A.
• HPLC high performance liquid chromatography
• the cleaner composition is sampled into clean containers, immediately after it is manufactured, or after it is stored at room temperature or at elevated temperatures.
• the samples are sent to a certified analytic laboratory for analysis of the formaldehyde content. It is known that in some aqueous compositions stored under elevated temperatures, or stored at room temperature for a substantial period of time, the formaldehyde concentration may increase from the level when it is freshly made.
• Various storage conditions that may occur during product shipping and customer uses for example storing at room temperature and at elevated temperatures up to 40 0 C, have been tested.
• the cleaner composition of the present invention is substantially free of formaldehyde.
• the formaldehyde concentration of the cleaner composition is less than 1 ppm when it is freshly made, and remains less than 1 ppm under various storage conditions.
• the present invention provides a method of cleaning, or maintaining a blood analyzer using the formaldehyde-free cleaner composition of the present invention.
• blood analyzer used herein refers to automated or semi-automated instruments used for analyzing blood samples, such as hematology analyzers, flow cytometers, and clinical chemistry analyzers.
• the cleaner composition is automatically delivered by an automated blood analyzer to the components or parts of the instrument, which contact the sample mixture formed by a blood sample and one or more reagent, during blood analysis, the cleaner composition is kept in contact with these instrument components for a period of time, and rinsed out with a diluent or water.
• air-mixing the cleaner composition by air flow or bubbling can also be utilized to increase physical agitation on the surfaces of the components.
• the cleaner composition removes precipitates built up on the surfaces of the components, which are generated from debris, or protein precipitates from lysed blood samples, or chemical complexes formed between blood components and the reagents used for blood analysis; therefore, it maintains the instrument components clean and prevents undesired build-ups on the surfaces of these components.
• the cleaning process can be carried out one or more times daily, depending on the usage or workload of the instrument.
• the cleaner composition On a hematology analyzer, the cleaner composition is usually delivered to the instrument components, such as chambers for preparing the blood sample, flow cells, apertures and tubings which are in contact with the sample mixture during analysis, after regular use of the instrument, typically at the instrument shutdown time.
• the contact time, or incubation time, of the cleaner composition with the instrument components can be programmed on the automated instrument, typically from 10 minutes to 12 hours, and can be even longer.
• the instrument typically at the shutdown time the instrument is rinsed one or more times with the cleaner composition, and the cleaner composition is delivered again and maintained in the chambers and the tubings overnight, then rinsed out from the system at the start up time in the next day.
• the cleaning process is also commonly performed once at the end of each shift in clinical hematology laboratories, when the instruments are used for all three shifts a day.
• an automated cleaning cycle can also be programmed into the instrument system, which automatically carries out the cleaning process after a predetermined number of samples have been analyzed on the instrument.
• the cleaner composition is typically rinsed out using a blood diluent which is used for blood analysis in the blood analyzer. However, it can also be rinsed out by water.
• the frequency of the cleaning process and the contact time of the cleaner composition with the instrument components can be determined by the instrument manufacturer based on the instrument's needs, and it can also be determined by the laboratories based on the workload of the instrument.
• Example 4 illustrates an example using a cleaner composition of the present invention for maintaining a Coulter LH750 hematology analyzer (product of Beckman Coulter, Inc., Fullerton, California). As shown, after running about 1 ,100 blood analysis cycles under a stressed instrument working environment in a period of two weeks, no precipitation on the surface around the apertures was observed.
• a Coulter LH750 hematology analyzer product of Beckman Coulter, Inc., Fullerton, California
• Formaldehyde concentration 0.4 ppm
• Formaldehyde concentration 0.6 ppm
• Preservative Effectiveness Test was performed on Formula A of Example 1 to determine antimicrobial effectiveness of the cleaner composition.
• the Preservative Effectiveness Test is a measure of the ability of an antimicrobial to limit the proliferation of bacterial, yeast and mold cells and/or spores at or below the levels and conditions stated in the USP 26 (United States Pharmacopeia 26, Chapter 51 ) Preservative Effectiveness Test.
• the cleaner composition was first tested for initial sterility by inoculating 0.5 ml of the cleaner composition into nutrient broth tubes and incubating for seven days at 20-25 0 C and 30-35 0 C. No organism was observed in the incubated samples.
• the cleaner composition was tested against eleven organisms including six bacteria, two yeasts, and three fungi. Five of the organisms were listed in USP 26, and six organisms were previously isolated from hematology analyzers having contamination from organism growth. The organisms were prepared according to microbiology standard procedures to obtain initial suspensions containing approximately 1x10 7 to 1x10 8 CFU (colony forming units)/ml. 10 ml of a cleaner composition in a sample tube was inoculated with 50 ⁇ l of the initial suspension for each of the organisms to obtain a concentration of each organism in the cleaner composition between 1x10 5 and 1x10 6 CFU/ml. The inoculation levels used in the preservative effectiveness test were shown in Table 1.
• the inoculated sample tubes were mixed and incubated according to USP 26.
• the inoculated cleaner compositions were diluted in the saline blank, and plated out using standard plate count methodology.
• the plates were incubated according to the microbial recovery incubation times in USP 26. Following incubation, colony forming units were enumerated and reported.
• the inoculated cleaner composition was evaluated at 48 hours, 7, 14, 21 and 28 days. The results at 48 hours, 7, 14 and 28 days are shown in Table 2. As shown, no growth of all organisms tested was observed in Formula A. It was noted that the inoculation levels were reduced to less than 10 2 (CFU/ml) as early as 48 hours, and were maintained below 10 2 (CFU/ml) during the 28 day test period. The results showed that the antimicrobials in Formula A were effective in preserving the cleaner composition.
• Formula B As shown, no growth of all organisms tested was observed in Formula B.
• the antimicrobials in Formula B were effective in eliminating all organisms tested in a 48 hour period of time, which was substantially shorter than 7 days as required by USP 26.
• Formula A of Example 1 was sampled at various times, including right after the cleaner composition was made, referred to as fresh, and after incubating the cleaner composition at 45°C for 17 and 35 days. Incubation at the elevated temperature provided an accelerated reagent stability test condition. The samples were sent to Adirondack Environmental Services, Inc., Albany, New York, for analysis. Adirondack Environmental Services, Inc. is certified by National Environmental Laboratory Approval Program. Formaldehyde concentration of the cleaner composition was analyzed using HLPC according to the procedure described in EPA Method 8315A. The analysis results are shown in Table 4.
• the formaldehyde concentration maintained less than 1 ppm.
• a Coulter LH750 hematology analyzer (product of Beckman Coulter, Inc., Fullerton, California) was used in a performance test of the cleaner composition of the present invention.
• the Coulter Clenz ® an existing cleaner product of Beckman Coulter, Inc., connected to instrument was replaced by Formula A of Example 1.
• An eleven working day stress test was performed on the hematology analyzer, which simulated a stressed instrument working environment. During the eleven day test period, there were two weekends. Each day, after the instrument's regular start up cycle, a quality control procedure was performed, as recommended to the instrument users, which involved testing various blood control products.

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