WO2007025467A1

WO2007025467A1 - A liquid-phase chromatographic separating column

Info

Publication number: WO2007025467A1
Application number: PCT/CN2006/002209
Authority: WO
Inventors: Ke Wang
Original assignee: Ke Wang
Priority date: 2005-08-27
Filing date: 2006-08-28
Publication date: 2007-03-08
Also published as: CN2815464Y

Abstract

A liquid-phase chromatographic separating column comprises a column head and a column body. The column body constitutes of a barrel , solid-phase adsorbents filled in the barrel and filter discs located at both sides of the adsorbents which can prevent the adsorbents leaking and allow flowing phase to pass through them. One end of the barrel has a liquid outlet and the other end is a inlet end. The column head includes a head body with a feed inlet, a shell cover, a wedge and an outer round wall disposed on the shell cover axially. The head body is installed on the side of the barrel’s open end and connects with the inner wall of the barrel hermetically. The outer round wall on the shell cover connects with the open end of the barrel and the wedge is clipped in the groove of the head body.

Description

Liquid chromatography separation column technology.

The present invention relates to a liquid chromatography separation column. Background technique

Liquid chromatography separation techniques are used to efficiently separate mixtures of mixtures with multiple components, the core of which is a liquid chromatography column. The principle of separation is that a sample of the mixture is dissolved in a mobile phase and flows through the stationary phase adsorbent filled in the chromatographic separation column at a certain rate, because the adsorption force of the stationary phase adsorbent on different components is different. Therefore, the mixed components will flow through the stationary phase adsorbent at different rates to achieve the purpose of separation. After using this chromatographic column once, if it needs to be reused, it is necessary to replace the new stationary phase adsorbent, which is not only troublesome, inefficient, but also affects the health of the operator. In order to improve work efficiency and reduce the harm of powdered stationary phase adsorbents to human health, disposable pre-filled chromatography columns have been increasingly used in recent years. However, the existing disposable chromatographic separation column has the following problems in design: 1. High manufacturing cost and high cost; 2. Mobile phase leakage may occur during pressure operation and the chromatographic head of the chromatographic separation column may fall off. Summary of the invention

The invention provides a column head body of the chromatographic column head which can be reliably sealed and connected with the cylinder body under pressure under the pressure of the cylinder body, and does not cause mobile phase leakage under a large working pressure, and does not occur. A liquid chromatography column for column head detachment.

The technical solution of the present invention to achieve the above object is: a liquid chromatography separation column comprising a separation column body and a chromatography column head, the separation column body is composed of a cylinder, a stationary phase adsorbent filled in the cylinder body, and a capping phase in the stationary phase a filter for preventing leakage of the stationary phase adsorbent at both ends of the adsorbent and capable of passing the mobile phase, wherein the tubular body has a liquid outlet at one end and an open end at the other end of the cylinder. The column head comprises a column head body with a liquid inlet, an outer ring wall axially disposed on the end cover and a mast, the column head body being press-fitted on the open end side of the barrel body and sealingly connected with the inner wall of the barrel body, the end cap The upper outer ring wall is connected to the open end of the cylinder, and the mast is crimped onto the notch of the stud body. The chromatographic column head of the invention consists of a split stigma body and an end cap, and is sealed and connected with the barrel body through the column head body. When the liquid chromatographic separation column is used under pressure, the stigma body receives a back pressure due to the back pressure in the cylinder body. When the force of the cylinder is separated from the cylinder, when the column head body is slightly moved outward, the column head body is pressed against the notch of the column head body, so that the column head body can only produce radial deformation under the allowable working pressure, and the pressure in the cylinder body is higher. Larger, the greater the radial deformation of the stud body, the tighter the seal connection between the stud body and the barrel. The invention has simple structure, is convenient for industrial production, and reduces manufacturing cost; the liquid chromatography separation column adopting the structure can be used once or repeatedly, and the structure effectively solves the problem of the cylinder and the chromatographic column head. The problem of detachment after pressure, therefore, the chromatographic separation column of the present invention can withstand greater working pressure without causing leakage of the mobile phase. The chromatographic separation column of the invention can be applied to a variety of manual or automatic fast liquid chromatography separation systems as a general-purpose color separation column, and has the advantages of simple operation, low use cost, and greatly improved production efficiency. DRAWINGS

The embodiments of the present invention are further described in detail below with reference to the accompanying drawings.

Figure 1 is a schematic view of the structure of the present invention.

Fig. 2 is a schematic enlarged view of the structure of Fig. 1;

Wherein, 1 column head; 11 inlet port; 12-column body; 13-end cover; 14 outer ring wall; 15-column; 16-notch; 17-radial groove; 3 - separation column body; 31 - cylinder: 32 - stationary phase adsorbent; 33 - liquid outlet; 34 - reinforcing rib; 35 - end; 4 - filter. detailed description

The liquid chromatography separation column shown in Fig. 1 includes a separation column body 3, a filter 2, 4, and a column head 1. The separation column body 3 is composed of a cylindrical body 31 and a stationary phase adsorption 32 filled in the cylindrical body 31, and a filter for preventing the stationary phase adsorbent 32 from leaking out at both ends of the stationary phase adsorbent 32 and allowing the mobile phase to pass therethrough. 2 and filter 4 are composed. The cylinder 31 of the present invention is made of a high density plastic or other organic solvent resistant material, and the filters 2, 4 are generally made of a high density hydrophobic microporous plastic.

As shown in Fig. 1, the cylindrical body 31 of the separation column body 3 of the present invention has a liquid outlet 33 at one end thereof and an open end at the other end of the cylinder 31. The chromatographic column head 1 of the present invention comprises a stud body 12 with a liquid inlet port 11, an end cap 13 and an outer ring wall 14 and a mast 15 disposed axially on the end cap 13 as shown in Figs. The mast 15 is arranged according to the geometry of the end cap 13. The mast 15 of the present invention is coaxial with the outer ring wall 14 and may be disposed for different axes. The stud body 12 and the end cap 13 may also be made of high-density plastic or other organic solvent-resistant material. The stud body 12 is press-fitted on the open end side of the barrel 31 and is sealingly connected to the inner wall of the barrel 31, preferably the diameter of the stud body 12. It is larger than the inner wall diameter of the cylinder 31 by 0.1 mm to L5 mm. Of course, the diameter of the column head body 12 may be equal to the diameter of the inner wall of the cylinder 31, and the matching visual material between the column head body 12 and the cylinder 31 and the pressure bearing capacity may be determined. When the stud body 12 is pressed into the cylindrical body 31, the stud main body 12 cooperates with the inner wall of the cylindrical body 31 to achieve a sealed connection, and the end surface of the stud main body 12 on the side of the filter 2 may be provided with a radially outwardly diffusing groove 17 The groove 17 is evenly disposed, and the end of the groove 17 communicates with the liquid inlet port 11, which acts to allow the mobile phase liquid of the liquid inlet to flow more uniformly through the stationary phase adsorbent. As shown in Figs. 1 and 2, the outer ring wall 14 on the end cap 13 is attached to the open end side of the cylindrical body 31, and the mast 15 is crimped to the notch 16 of the stud body 12. When there is a certain back pressure in the inner cavity of the cylinder 31, the notch 16 of the stud body 12 is supported by the mast 15 of the end cover 13, so that the stud body 12 is radially deformed, and the sealing connection with the cylinder 31 is tighter. Thereby, the reliable connection between the column head 1 and the barrel 31 is achieved, so that the liquid chromatography column can withstand a larger working pressure.

As shown in FIG. 1 and 2, the end cap 13 of the chromatographic column head 1 of the present invention has an internal thread on the outer ring wall 14 and is connected to the outer side wall of the open end of the cylinder 31 to be connected thereto. The present invention is in the end cap 13 The outer ring wall 14 can also be provided with a card slot or a buckle, and is connected with a corresponding buckle or a card slot on the outer side wall of the cylinder 31 to firmly connect the end cap 13 of the column head 1 with the barrel 31.

The notch 16 on the stud body 12 of the present invention is a communicating ring groove, and the mast 15 of the end cover 13 is an annular wall as shown in FIG. 2, and has two inclined faces symmetrically disposed, and the two inclined faces are pressed against the stigma. The sill 15 of the end cap 13 is an annular wall and has a unilateral outer slant which is pressed against the notch 16 of the stud body 12, the raft on the end cap 13 of the present invention. The length of the column 15 can be adjusted, and the large-diameter portion of the slope is connected to the inner side surface of the end cover 13, or the large-diameter portion of the inclined surface thereof is connected to the annular wall as shown in FIG. The notch 16 on the stud body 12 of the present invention may also be a discontinuous, symmetrically disposed notch 16, and the mast 15 on the end cap 13 is correspondingly disposed, and the groove on the stud body 12 is pressed by the inclined surface of the mast 15 On the mouth 16 Under the action of the inclined surface of the mast 15 of the end cap 13, once the stud body 12 is moved outwardly from the cylinder 31 by the back pressure of the cylinder, the stud head 15 is inclined by the bevel 15 of the end cap 13 to make the stud body The notch 16 of the 12 is radially deformed to form a tighter fit with the barrel 31, which improves the pressure bearing capacity of the column head 1 and the separation column body 3. As shown in FIG. 1, the present invention is provided with a reinforcing rib 34 on the inner end surface of the cylindrical body 31 on the side of the liquid outlet 33. The reinforcing rib 34 is outwardly lowered from the liquid outlet, and the end surface of the rib 34 is reinforced. In connection with the filter 4, on the one hand, the mechanical strength of the liquid outlet of the cylinder 31 can be improved, and on the other hand, since the end surface of the reinforcing rib 34 can be in contact with the filter 4, when the filter 4 is under pressure, the convexity is strengthened. The support of the ribs 34 is less prone to deformation.

As shown in Fig. 1, when assembling the liquid chromatography separation column, the filter 4 is first pressed into the cylinder 31, then loaded into the stationary phase adsorbent 32, pressed into another filter 2, and finally pressed into the chromatogram. The column head 1 first presses the column head body 12 into the cylinder body 31 to be sealed and connected, and then the end cap 13 is screwed or snapped to the open end of the barrel body 31, so that the column head 1 is firmly connected to the barrel 31, and works. The column head 1 and the separation column body 3 can achieve a reliable sealing performance and can withstand a large working pressure.

Claims

Rights request

A liquid chromatography separation column comprising a separation column body and a chromatographic column head, the separation column body being composed of a cylinder, a stationary phase adsorbent filled in the cylinder body, and a stationary phase adsorption adsorbent capped at both ends of the stationary phase adsorbent a filter that leaks out and enables the mobile phase to pass through, characterized in that one end of the cylinder is provided with a liquid outlet, and the other end of the cylinder is an open end, and the column head includes a liquid inlet The stud body, the end cap and the outer ring wall axially disposed on the end cap and the mast, the stud body is press-fitted on the end side of the barrel and sealingly connected with the inner wall of the cylinder, and the outer ring wall and the cylinder on the end cap The open end is connected, and the mast is crimped onto the notch of the stud body.

2. The liquid chromatography separation column according to claim 1, wherein the notch on the column head body is a communicating ring groove.

3. The liquid chromatography separation column according to claim 1, wherein: the notch on the column head body is a discontinuously symmetric notch.

4. The liquid chromatography separation column according to claim 1, wherein: the end of the column head body on one side of the filter body is provided with a radially outwardly diffusing groove, and the end of the groove and the liquid inlet port. The same.

The liquid chromatography separation column according to any one of claims 1 to 4, wherein: the mast on the end cover is an annular wall, and has two inclined surfaces symmetrically disposed, and the two inclined surfaces are crimped at On the notch of the stigma body.

The liquid chromatography separation column according to any one of claims 1 to 4, wherein: the mast on the end cover is an annular wall and has a single outer inclined surface, and the outer inclined surface is crimped to the stud On the notch of the body.

7. The liquid chromatography separation column according to claim 1, wherein: the outer ring wall of the end cap is provided with an internal thread, and is screwed to the outer wall of the cylinder to be connected to the cylinder.

The liquid chromatographic separation column according to claim 1, wherein: the outer ring wall of the end cover is provided with a card slot or a buckle, and a corresponding buckle or card slot on the outer wall of the outer tube Lock connection.

The liquid chromatographic separation column according to any one of claims 1 to 4, wherein the diameter of the column head body is larger than the inner wall diameter of the cylinder by l to 1.5 mm.

10. The liquid chromatography separation column according to claim 1, wherein: the cylinder is located The inner end surface of the liquid outlet is provided with a reinforcing rib, and the reinforcing rib is descended from the liquid outlet, and the end surface of the reinforcing rib is connected with the filter.