JP2015114293A - Column for liquid chromatograph and liquid chromatograph apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a technique capable of replacing a clogged filter without changing the filling state of filler in a column and easily solving the problem of poor analysis accuracy.SOLUTION: A column for liquid chromatograph includes a filter disposed at the entrance side of a column 5. The filter comprises a two-layer structure of an outside filter 54 and an inside filter 53 from the inflow direction of a sample to be analyzed in order. The outside filter 54 is replaceable. The inside filter 53 is attached onto a column body 52. The inside filter 53 plays a role to preserve filler in the column 5. The outside filter 54 plays a role to prevent impurities in the sample or in a mobile phase from flowing into the column 5.

Description

  The present invention relates to a column used for a liquid chromatograph and a liquid chromatograph apparatus provided with the column.

  A liquid chromatograph is an analyzer widely used in fields such as food, chemistry, pharmaceuticals, and clinical chemistry. In this liquid chromatograph, it is possible to perform qualitative and quantitative analysis by separating components in a sample to be analyzed.

  In many cases, a sample to be analyzed is analyzed after impurities are removed through pretreatment of various processes. However, since it is difficult to completely remove impurities and the preprocessing is a complicated operation, the analysis may be started by the operator unfamiliar with the analysis without performing the preprocessing correctly.

  As a result, when impurities in the sample enter the column, there is a possibility that the pressure increases due to a decrease in analysis accuracy or the column is clogged, resulting in a state where analysis is impossible.

  When foreign matter such as dust accumulates on the column filter during analysis and clogging occurs, it is sometimes necessary to stop the operation and interrupt the analysis.

  As a solution to this problem, for example, Patent Document 1 describes a filter having a first filter made of glass and a second filter made of metal in order to increase the filtration capacity of impurities in the column. For example, Patent Document 2 describes a filter having a configuration in which filter paper, one or more membrane filters, and a sintered filter are provided.

JP 2013-3015 A JP 2011-242238 A

  However, in the configuration of the filter described in Patent Document 1 described above, since the double filter of the first filter and the second filter is fixed as one body, the handling thereof is inconvenient. For example, since it is necessary to always remove both filters each time one filter is replaced or washed, the packing in the column may be released, and the packing state may change.

  Moreover, in the structure of the filter described in Patent Document 2 described above, a filter having a structure provided with filter paper, one or more membrane filters, and a sintered filter is accommodated in the filter case, and the entire filter case is replaced. Become. For this reason, also in the structure of the filter described in Patent Document 2, as in Patent Document 1, the packing in the column is released, and the packing state may change.

  As described above, when the column packing state changes, the performance of the column is directly affected. Therefore, the separation performance is degraded, and the quantitative result is adversely affected, leading to a decrease in reliability of the column quality.

  In addition, since foreign matters such as dust flow into the column together with the mobile phase, the filter on the inlet side of the column is often clogged. Therefore, in order to return the column once clogged with the filter to the original state, the mobile phase is flowed in the reverse direction, that is, by flowing the mobile phase from the column outlet side, so that dust accumulated on the filter on the column inlet side There is a case where an operation of cleaning by extruding foreign matter is performed.

  This countermeasure is effective when the degree of clogging of the filter is mild, but otherwise it is not a fundamental solution, and eventually the liquid permeability of the column does not return to the original state. . As described above, when the mobile phase does not return to the column even when flowing through the column from the opposite direction, it is necessary to replace the filter or replace the column itself.

  Therefore, the present invention has been made in view of the above problems, and its typical purpose is to replace the clogged filter without changing the packing state of the packing material in the column, and to reduce the analysis accuracy. The purpose is to provide a technology that can easily solve the problem.

  The above and other objects and novel features of the present invention will be apparent from the description of this specification and the accompanying drawings.

  Of the inventions disclosed in the present application, the outline of typical ones will be briefly described as follows.

  That is, a typical column for liquid chromatography is a column for liquid chromatography, and has a filter disposed on the inlet side of the column. The filter has a two-layer structure of an outer filter and an inner filter in order from the inflow direction of the sample to be analyzed, and the outer filter can be exchanged.

  More preferably, the inner filter is bonded to the column body.

  More preferably, the inner filter serves to retain the packing material in the column, and the outer filter serves to prevent impurities in the sample or mobile phase from flowing into the column. .

  Further, the present invention is also applied to a liquid chromatograph apparatus provided with the liquid chromatograph column.

  Among the inventions disclosed in the present application, effects obtained by typical ones will be briefly described as follows.

  That is, a typical effect is that the clogged filter can be replaced without changing the packing state of the packing material in the column, and the problem of reduced analysis accuracy can be easily solved.

It is a block diagram which shows an example of the outline | summary of the liquid chromatograph apparatus in one embodiment of this invention. FIG. 2 is a cross-sectional view showing a part of an example of a liquid chromatograph column in the liquid chromatograph apparatus shown in FIG. 1. FIG. 3 is a cross-sectional view showing an example of a state where an outer filter is removed from the liquid chromatograph column shown in FIG. 2. FIG. 3 is an explanatory diagram illustrating an example of a change in pressure with respect to the number of analysis times of a sample in an analysis experiment using the column according to the present embodiment illustrated in FIG. It is explanatory drawing which shows an example of the area value reproducibility regarding the component peak at the time of conducting the analytical experiment shown in FIG.

  In the following embodiments, when it is necessary for the sake of convenience, the description will be divided into a plurality of sections or embodiments. However, unless otherwise specified, they are not irrelevant and one is the other. There are some or all of the modifications, details, supplementary explanations, and the like. Further, in the following embodiments, when referring to the number of elements (including the number, numerical value, quantity, range, etc.), especially when clearly indicated and when clearly limited to a specific number in principle, etc. Except, it is not limited to the specific number, and may be more or less than the specific number.

  Further, in the following embodiments, the constituent elements (including element steps and the like) are not necessarily indispensable unless otherwise specified and apparently essential in principle. Needless to say. Similarly, in the following embodiments, when referring to the shapes, positional relationships, etc. of the components, etc., the shapes are substantially the same unless otherwise specified, or otherwise apparent in principle. And the like are included. The same applies to the above numerical values and ranges.

[Outline of the embodiment]
First, an outline of the embodiment will be described. In the outline of the present embodiment, as an example, the reference numerals of the corresponding components of the embodiment are given in parentheses.

  A typical liquid chromatograph column of the present embodiment is a liquid chromatograph column, and has a filter disposed on the inlet side of the column. The filter has a two-layer structure of an outer filter (outer filter 54) and an inner filter (inner filter 53) in order from the inflow direction of the sample to be analyzed, and the outer filter is replaceable. is there.

  More preferably, the inner filter is bonded to a column body (column body 52).

  More preferably, the inner filter serves to retain the packing material in the column, and the outer filter serves to prevent impurities in the sample or mobile phase from flowing into the column. .

  Further, the present invention is also applied to a liquid chromatograph apparatus provided with the liquid chromatograph column.

  Hereinafter, an embodiment based on the outline of the above-described embodiment will be described in detail with reference to the drawings. Note that components having the same function are denoted by the same reference symbols throughout the drawings for describing the embodiment, and the repetitive description thereof will be omitted. In the embodiments, the description of the same or similar parts will not be repeated in principle unless particularly necessary.

  In the drawings used in the embodiments, hatching may be omitted even in a cross-sectional view so as to make the drawings easy to see. Further, even a plan view may be hatched to make the drawing easy to see.

[One Embodiment]
A liquid chromatograph column in the present embodiment and a liquid chromatograph apparatus including the column will be described with reference to FIGS. However, the following embodiment is merely an example for realizing the present invention, and is not limited thereto.

<Liquid chromatograph>
FIG. 1 is a configuration diagram showing an example of an outline of a liquid chromatograph apparatus in the present embodiment.

  The liquid chromatograph apparatus in the present embodiment includes mobile phases 1A and 1B, electromagnetic valves 2A and 2B, a pump 3, a sample introduction apparatus 4, a column 5, a detector 6, a data processing apparatus 7, and the like. In this liquid chromatograph apparatus, each component device is controlled by a control device (not shown).

  The mobile phases 1A and 1B are mobile phases such as a plurality of eluents. Here, two examples of mobile phases 1A and 1B are shown so that gradient elution is possible. The mobile phases 1 </ b> A and 1 </ b> B are liquids that flow through the column 5, and by flowing this liquid, the mixture contained in the sample is separated in the column 5 and discharged from the column 5 in order.

  The electromagnetic valves 2A and 2B are electromagnetic valves that are provided corresponding to the mobile phases 1A and 1B and selectively switch the supply of the mobile phases 1A and 1B.

  The pump 3 is a pump for supplying the mobile phases 1A and 1B selected by switching the electromagnetic valves 2A and 2B toward the column 5 at a constant flow rate and column pressure.

  The sample introduction device 4 is a sample introduction device for introducing a sample to be analyzed and supplying the sample to the column 5 together with the mobile phases 1A and 1B.

  The column 5 is filled with a filler, and is a column for separating the sample introduced together with the mobile phases 1A and 1B from the sample introduction device 4 for each component and sequentially discharging the sample to the detector 6. Further, on the input side of the column 5, a filter having a two-layer structure stacked in the inflow direction of the sample introduced together with the mobile phases 1A and 1B is arranged, as will be described in detail later.

  The detector 6 is a detector for detecting separated components discharged from the column 5.

  The data processing device 7 is a data processing device for performing qualitative / quantitative analysis of a sample based on the detection results of the components detected by the detector 6.

  In the liquid chromatograph as described above, the operation for separating the components in the sample to be analyzed and performing the qualitative / quantitative analysis is as follows. The operation of performing the qualitative / quantitative analysis is executed by a control device that controls each component device. For example, a computer system or the like is used for this control device, and each process of operation is automatically performed by executing an analysis program.

  First, one of the mobile phases 1A and 1B is selected by the electromagnetic valves 2A and 2B. For example, when the mobile phase 1A is selected by the electromagnetic valve 2A, the selected mobile phase 1A is supplied by the pump 3 toward the sample introduction device 4 at a constant flow rate and column pressure. A sample to be analyzed is introduced into the sample introduction device 4.

  Then, the sample is introduced into the column 5 from the sample introduction device 4 together with the mobile phase 1A. In the column 5, impurities in the mobile phase 1 </ b> A and the sample introduced together with the mobile phase 1 </ b> A are filtered by a filter and introduced into the column 5. The sample introduced into the column 5 is separated for each component by moving through the column 5 as the mobile phase 1 </ b> A flows, and is sequentially discharged from the column 5.

  Furthermore, each component discharged | emitted from the column 5 is detected by the detector 6 in order. Then, the data processing device 7 performs qualitative / quantitative analysis of the sample based on the detection result by the detector 6.

  Similarly, when the mobile phase 1B is selected by the electromagnetic valve 2B, the sample is introduced into the column 5 together with the selected mobile phase 1B, separated into components, and sequentially discharged. Each component is detected by the detector 6. Is detected.

  As described above, in the liquid chromatograph apparatus, the sample is separated into components by flowing the sample in the column 5 together with the mobile phases 1A and 1B, and these components are detected by the detector 6, and the detection result is obtained. Qualitative and quantitative analysis can be performed based on this.

<Column for liquid chromatography>
FIG. 2 is a cross-sectional view showing a part of an example of a column for liquid chromatography in the liquid chromatograph apparatus shown in FIG. FIG. 3 is a cross-sectional view showing an example of the liquid chromatograph column shown in FIG. 2 with the outer filter removed.

  The liquid chromatograph column 5 in this embodiment includes an end fitting 51, a column body 52, an inner filter 53, an outer filter 54, an inner packing 55, an outer packing 56, a packing material 59, and the like. . In FIG. 2, the end fitting on the output side is omitted, but the end fitting is joined to both ends of the column body 52 on the input side and the output side. In the column 5, an end fitting 51 is joined to the input side of the tubular column body 52, and an end fitting (not shown) is joined to the output side of the column body 52.

  A filter having a two-layer structure of an inner filter 53 and an outer filter 54 is disposed at a joint portion between the end fitting 51 and the column body 52. This two-layer filter includes an inner filter 53 and an outer filter 54 that are independent from each other, and has a structure in which the sample and the mobile phase are stacked in the inflow direction. Regarding these two-layer filters, the upstream filter in the inflow direction is referred to as the outer filter 54, and the downstream filter is referred to as the inner filter 53.

  The inner filter 53 plays a role of holding the packing material 59 in the column 5, and the outer filter 54 plays a role of preventing impurities from flowing into the column 5. The inner filter 53 is bonded to the column body 52 by some means. Specific bonding means can be bonded by an adhesive, welding or the like, but are not limited to these means as long as the function as the liquid chromatograph column 5 is not impaired. On the other hand, the outer filter 54 is held by a structure that can be removed freely and can be easily replaced. An end fitting 51 is joined as a structure for holding the outer filter 54. FIG. 3 shows a state where the end fitting 51 is removed and the outer filter 54 is removed.

  Between the inner filter 53 and the end fitting 51, a packing 55 as an inner seal member is disposed. Between the outer filter 54 and the end fitting 51, a packing 56 as an outer seal member is disposed. These inner and outer packings 55 and 56 make it possible to prevent leakage of the sample and mobile phase introduced from the input side of the column 5. As shown in FIG. 3, in the state where the end fitting 51 is removed, in addition to the outer filter 54, the inner and outer packings 55 and 56 can also be removed. The packing need not necessarily be divided into two, and may be one. Further, only the outer packing 56 or the inner packing 55 may be used.

  The end fitting 51 and the column body 52 are joined by, for example, a screw method. The threaded portion 57 between the end fitting 51 and the column body 52 has a male thread cut in the column body 52 and is directly joined to the female thread cut in the end fitting 51. The end fitting 51 has a female thread as a threaded portion 58 for connecting to the pipe on the side opposite to the column body 52.

  An outlet side filter is also arranged at the junction between the column body 52 and the output side end fitting. The outlet side filter needs to have a structure and function that exceed the scope of the prior art. There is no. That is, the filter on the outlet side may serve to hold the packing material 59 in the column 5.

  Thus, the column 5 is a tubular column body 52 filled with the filler 59 and sealed at both ends with the input side filter and the output side filter. Further, the periphery of the filter on the input side and the output side is pressed with a packing to prevent leakage, and the end fittings on the input side and the output side are screwed into the column body 52, so that the filters on the input side and the output side are filled with the filler 59. To be sealed.

  In FIG. 2, the arrows indicate the directions in which the mobile phase flows when the column 5 of the present embodiment is attached to a liquid chromatograph device. Foreign matter in the sample to be analyzed or in the mobile phase accumulates in the outer filter 54. The inner filter 53 is a filter for holding the packing material 59 in the column 5. The pore diameter of the inner filter 53 is preferably smaller than the particle diameter of the filler 59. The material of the inner filter 53 is, for example, stainless steel SUS316, but is not limited thereto. The hole diameter of the outer filter 54 is preferably equal to or smaller than the hole diameter of the inner filter 53. The material of the outer filter 54 is, for example, a membrane filter, but is not limited to this.

  The inner filter 53 and the column body 52 are bonded by an adhesive, welding or the like. When the end fitting 51 is removed, the outer filter 54 and the packings 55 and 56 are removed together with this, but the inner filter 53 is kept adhered to the column body 52, so that the filler 59 does not leak out. Further, the outer filter 54 can be easily removed and replaced by removing the end fitting 51.

  Conventionally, when packing a packing material into a column, a filter and an end fitting are attached to the outlet side, and the packing material is packed in the same direction as when the analysis is performed, that is, in the same direction as the direction in which the mobile phase flows. It was. For this reason, conventionally, when the filter on the inlet side is bonded to the column body in advance, the column cannot be filled.

  In the present embodiment, in the two-layered filter, a structure in which the inner filter 53 and the column body 52 are bonded is employed, and the column 5 starts from the direction opposite to the direction in which the mobile phase flows (from the outlet side of the column 5). The method of filling is used. That is, in the present embodiment, when filling the column 5 with the packing material 59, the filter 59 having the two-layer structure and the end fitting 51 are attached to the inlet side of the column 5, and then the packing material 59 is loaded from the outlet side of the column 5. Fill. Then, a filter and an end fitting are attached to the outlet side of the column 5 to seal the packing material 59.

<Changes in pressure with respect to the number of sample analyzes>
FIG. 4 is an explanatory diagram showing an example of a change in pressure with respect to the number of analysis times of a sample in an analysis experiment using the column of the present embodiment shown in FIG. 2 and the conventional column. In FIG. 4, the horizontal axis indicates the number of analyzes, the vertical axis indicates the pressure, and the change in pressure when the sample is measured a plurality of times. This pressure increases as the filter becomes more clogged. In this analysis experiment, as an example, the control serum was denatured by treatment with trichloroacetic acid and then centrifuged, and the supernatant was used as a sample.

  In the column of the prior art, the upper limit pressure at which the apparatus can be operated was exceeded at the time of the 700th analysis. Since the device was designed to stop when the upper limit pressure was exceeded, the continuous analysis was terminated.

  On the other hand, when continuous analysis was performed using the column 5 of the present embodiment, the upper limit pressure exceeded 25 MPa during the 640th analysis. Once the apparatus was stopped and the filter 54 outside the column 5 was replaced with a new one, the analysis was resumed again. Then, the pressure decreased to a value equivalent to that at the time of the first analysis, and thereafter, a pressure increase similar to that until the 600th analysis occurred.

<Area value reproducibility for component peaks>
FIG. 5 is an explanatory diagram showing an example of the area value reproducibility regarding the component peak when the analysis experiment shown in FIG. 4 is performed. In FIG. 5, the area value reproducibility regarding the component peak of aspartic acid contained in a sample is shown as an example.

  The first to fifth columns in FIG. 5 indicate the initial area value reproducibility at the start of analysis, and the 600th to 604th columns indicate the 600 to 604th area value reproducibility. This is a numerical value immediately before exchanging the outer filter 54 in the analysis experiment using the column 5 of the present embodiment described above. It can be seen that in both the conventional column and the column 5 of the present embodiment, the analysis accuracy when reaching 600 times is about 10 times worse than the initial analysis accuracy. In the example of FIG. 5, in the column of the prior art, the deterioration is from 0.4% to 9.5%, and in the column 5 of the present embodiment, the deterioration is from 0.3% to 5.7%.

  Furthermore, the 700th to 704th columns in FIG. 5 indicate the 700 to 704th area value reproducibility. For the prior art column, no results are shown because the experiment was canceled at the 700th time. In the column 5 of the present embodiment, the area value reproducibility at this time was 0.3%. This shows a value that is one digit lower than the numerical value of area value reproducibility (5.7%) at the 600th to 604th time, and is equivalent to the area value reproducibility at the start of analysis (0.3). %). This is considered to be the effect of the pressure being lowered to the original value by exchanging the outer filter 54.

<Effect of the embodiment>
As described above, according to the liquid chromatograph column 5 in the present embodiment and the liquid chromatograph apparatus including the column 5, typically, the packing state of the packing material 59 in the column 5 is changed. The clogged outer filter 54 can be replaced without any problem, and the problem of reduced analysis accuracy can be easily solved.

  That is, in the present embodiment, the problem that the analysis accuracy is lowered when the filter of the column 5 is clogged can be easily solved. Conventionally, when a column filter is clogged, (1) the column is washed by flowing a mobile phase from the opposite direction, (2) the end fitting on the inlet side is removed, and the filter is replaced (3 ) Countermeasures have been taken, such as repacking the column.

  However, (1) does not improve depending on the degree of clogging of the filter, (2) reduces the accuracy of the analysis because the packing state of the packing changes, and (3) reduces column accuracy. However, there is a problem that it usually takes about 2 to 3 hours and requires labor and labor.

  Therefore, in the present embodiment, in the filter on the input side of the column 5 having a two-layer structure of the outer filter 54 and the inner filter 53, the outer filter 54 can be replaced, so that the outer filter 54 can be replaced. When clogged, the outer filter 54 can be easily replaced. Further, since the inner filter 53 is bonded to the column body 52, the packing state of the filler 59 in the column 5 is not changed by the inner filter 53.

  In this way, the inner filter 53 can serve to hold the packing material 59 in the column 5. Further, the outer filter 54 can play a role of preventing impurities in the sample or the mobile phase from flowing into the column 5.

  In particular, the effect of retaining the filler 59 in the column 5 becomes even more remarkable by making the pore diameter of the inner filter 53 smaller than the particle diameter of the filler 59. Further, the effect of preventing impurities in the sample or the mobile phase from flowing into the column 5 by making the hole diameter of the outer filter 54 equal to or smaller than the hole diameter of the inner filter 53 is even more remarkable. It becomes.

  Further, even when the inner filter 53 is bonded to the column body 52 on the input side of the column 5, when filling the column 59 with the filler 59, the outlet side from the direction opposite to the direction in which the mobile phase flows is used. Filler 59 can be filled.

  Further, since the inner filter 53 and the end fitting 51 and the outer filter 54 and the end fitting 51 can be sealed with the inner packing 55 or the outer packing 56, respectively, they are introduced into the column 5. It is possible to prevent leakage of the sample and mobile phase.

<Modified example of filter having a two-layer structure>
In the embodiment described above, the example in which the hole diameter of the outer filter 54 is preferably equal to or smaller than the hole diameter of the inner filter 53 has been described. However, for example, the following modifications may be considered.

  For example, when focusing on the thickness of the filter, the hole diameter of the outer filter 54 is made smaller than the hole diameter of the inner filter 53, so that the thickness of the inner filter 53 is larger than the thickness of the outer filter 54. Can be thinned. The reason is that if the hole diameter of the outer filter 54 is made smaller, the ability to trap foreign matter in the sample and mobile phase is improved, and accordingly, the ability to trap foreign matter in the inner filter 53 can be weakened. .

  Thus, the merit when the thickness of the inner filter 53 is reduced is that the number of theoretical plates of the column 5 is improved (the performance of the column is improved) by reducing the dead volume. That is, it becomes possible to perform a more accurate analysis. The dead volume refers to the volume of the portion where the sample to be analyzed of the liquid chromatograph apparatus flows does not participate in the separation of the components in the sample (the portion involved in the separation is the filler 59 in FIG. 2). say.

  Accordingly, the dead volume can be reduced by reducing the thickness of the inner filter 53. As a result, the performance of the column 5 can be improved, and a more accurate analysis can be performed.

  In addition to this modification, between the outer filter 54 and the inner filter 53, the optimum hole diameter, thickness, and the like from the relationship of the hole diameter of each filter, the thickness of each filter, the material of each filter, etc. It is desirable to select the material and the like. Furthermore, since the hole diameter and thickness of each filter also affect the volume of each filter, it can also be defined by paying attention to the volume of each filter. For example, when the volume of the filter is reduced, the dead volume can be reduced as in the case where the thickness is reduced.

  As mentioned above, the invention made by the present inventor has been specifically described based on the embodiment. However, the present invention is not limited to the embodiment, and various modifications can be made without departing from the scope of the invention. Needless to say. For example, the above-described embodiment has been described in detail for easy understanding of the present invention, and is not necessarily limited to one having all the configurations described. In addition, it is possible to add, delete, and replace other configurations for a part of the configuration of the embodiment.

  For example, the liquid chromatograph apparatus is not limited to the configuration as shown in FIG. It goes without saying that various changes can be made, such as the configuration it has. The present invention can be widely applied to all liquid chromatograph apparatuses provided with an analytical column.

1A, 1B: Mobile phase 2A, 2B: Solenoid valve 3: Pump 4: Sample introduction device 5: Column 6: Detector 7: Data processing device 51: End fitting 52: Column body 53: Inner filter 54: Outer filter 55: Inner packing 56: Outer packing 57: Screw part 58: Screw part 59: Filler

Claims (15)

A column for liquid chromatography,
Having a filter disposed on the inlet side of the column;
The filter has a two-layer structure of an outer filter and an inner filter in order from the inflow direction of the sample to be analyzed,
The outer filter is a replaceable column for liquid chromatography. The liquid chromatograph column according to claim 1,
The inner filter is a liquid chromatographic column bonded to a column body. In the liquid chromatograph column according to claim 2,
The inner filter is responsible for holding the packing in the column;
The outer filter is a liquid chromatograph column that serves to prevent impurities in the sample or mobile phase from flowing into the column. In the liquid chromatograph column according to claim 3,
The pore size of the inner filter is smaller than the particle size of the filler,
The liquid chromatograph column has a pore diameter of the outer filter equal to or smaller than that of the inner filter. In the liquid chromatograph column according to claim 4,
The hole diameter of the outer filter is smaller than the hole diameter of the inner filter,
The liquid chromatograph column, wherein the inner filter is thinner than the outer filter. In the liquid chromatograph column according to claim 3,
A column for liquid chromatography, in which the packing material is packed from the direction opposite to the direction in which the mobile phase flows when the packing material is packed into the column. In the liquid chromatograph column according to claim 3,
An end fitting is joined to the input side of the column body,
An inner sealing member that seals between the inner filter and the end fitting;
An outer sealing member that seals between the outer filter and the end fitting;
A liquid chromatograph column further comprising: A liquid chromatograph apparatus provided with a column for liquid chromatograph,
The column has a filter disposed on the inlet side of the column;
The filter has a two-layer structure of an outer filter and an inner filter in order from the inflow direction of the sample to be analyzed,
The liquid chromatograph apparatus, wherein the outer filter is replaceable. The liquid chromatograph apparatus according to claim 8,
The inner filter is a liquid chromatograph apparatus bonded to a column body. The liquid chromatograph apparatus according to claim 9, wherein
The inner filter is responsible for holding the packing in the column;
The outer filter is a liquid chromatograph that plays a role of preventing impurities in the sample or mobile phase from flowing into the column. The liquid chromatograph apparatus according to claim 10, wherein
The pore size of the inner filter is smaller than the particle size of the filler,
The liquid chromatograph apparatus, wherein a hole diameter of the outer filter is equal to or smaller than a hole diameter of the inner filter. The liquid chromatograph apparatus according to claim 11, wherein
The hole diameter of the outer filter is smaller than the hole diameter of the inner filter,
The liquid chromatograph apparatus, wherein a thickness of the inner filter is thinner than a thickness of the outer filter. The liquid chromatograph apparatus according to claim 10, wherein
A liquid chromatograph apparatus that, when packed in the column, fills the packing material in a direction opposite to the direction in which the mobile phase flows. The liquid chromatograph apparatus according to claim 10, wherein
An end fitting is joined to the input side of the column body,
The column is
An inner sealing member that seals between the inner filter and the end fitting;
An outer sealing member that seals between the outer filter and the end fitting;
A liquid chromatograph apparatus further comprising: The liquid chromatograph apparatus according to claim 10, wherein
A sample introduction device for introducing the sample together with the mobile phase into the column;
A detector for detecting components separated in the column;
A data processing device for performing qualitative and quantitative analysis of the sample based on the detection result of the detector;
A liquid chromatograph apparatus further comprising:

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