CN102841166A - Liquid chromatographic column - Google Patents
Liquid chromatographic column Download PDFInfo
- Publication number
- CN102841166A CN102841166A CN2011101659953A CN201110165995A CN102841166A CN 102841166 A CN102841166 A CN 102841166A CN 2011101659953 A CN2011101659953 A CN 2011101659953A CN 201110165995 A CN201110165995 A CN 201110165995A CN 102841166 A CN102841166 A CN 102841166A
- Authority
- CN
- China
- Prior art keywords
- chromatographic column
- pressure cap
- groove
- joint
- column according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a liquid chromatographic column. A circle of groove is provided at the edge of a through hole which is at each of the inner sides of pressure caps of two ends where the two pressure caps are in bolt joint with a column tube; and a seal washer is arranged in each groove. By using the structure, the pressure cap is in reliable bolt joint with a joint, and the tube wall of the column tube can be tightly connected with the seal washer in the groove, thereby improving the tightness of the liquid chromatographic column, and prolonging the service life of the liquid chromatographic column.
Description
Technical field
The present invention relates to separation, the analysis technical field of chemicals, relate in particular to a kind of liquid-phase chromatographic column.
Background technology
The liquid chromatography isolation technics is to be used for effectively separating the blend sample with various ingredients, and core component is the liquid chromatography separating column.Its separation principle is; The blending ingredients sample is dissolved in a kind of moving phase, and moving phase flows through the chromatograph packing material that is seated in the chromatographic column with certain flow velocity under the condition of bigger pressure; Because chromatograph packing material is to the difference of the absorption affinity of different component; So different component will flow through chromatograph packing material with different flow rate, and detect time and each components contents that different component flows out chromatographic column in the detecting device of liquid chromatograph, thus the purpose that reaches separation, detects.Chromatographic column is general to adopt high-grade stainless steel to process.Sieve plate is sintered stainless steel or titanium alloy, and the aperture is 0.2～20um, in order to prevent the outflow of chromatograph packing material, prevents the Interference Detection effect.
But in liquid-phase chromatographic column commonly used, the joint location at two ends adopts bolt and the rigidly connected mode of nut usually.This kind connected mode is rigid connection of utilizing between metal covering and the face; Therefore if the wearing and tearing not high or that joint possibly take place when daily replacing solid phase adsorption filler of the machining precision of joint will cause whole chromatographic column gas leakage leakage, thereby cause this chromatographic column to use.Therefore, be necessary existing liquid phase adsorption chromatography column is further improved, with the impermeability that realizes improving the chromatographic column two ends, prolong chromatographic column serviceable life, reduce the purpose of the cost of liquid chromatographic detection.
Summary of the invention
The object of the present invention is to provide a kind of liquid-phase chromatographic column, its can realize improving the chromatographic column two ends impermeability, prolong chromatographic column serviceable life, reduce the cost of liquid chromatographic detection.
For realizing above-mentioned purpose, the invention provides a kind of liquid-phase chromatographic column, comprising: the through hole edge of the medial surface that column jecket, pressure cap, joint, chromatograph packing material, sieve plate, said pressure cap and column jecket join is provided with a circle groove.
As further improvement of the present invention, be provided with a sieve plate in the middle of the groove of said pressure cap.
As further improvement of the present invention, the groove width of the groove of said pressure cap is greater than the wall thickness of the arbitrary end of column jecket end column jecket.
As further improvement of the present invention, be provided with packing washer in the groove of pressure cap.
As further improvement of the present invention, be provided with an annular projection portion near the end at said column jecket two ends, the maximum outside diameter of annular projection portion is greater than the aperture of the minimum of joint.
As further improvement of the present invention, said joint has internal thread, and pressure cap has external thread, and both pitch equate.
As further improvement of the present invention, the stroke of said joint internal thread is greater than the externally threaded stroke of pressure cap.
As further improvement of the present invention, the external thread of said pressure cap and the internal thread of joint reliably are spirally connected, and the ring-type post jamb of column jecket closely contacts with packing washer in the pressure cap groove.
As further improvement of the present invention, said packing washer can (Poly Tetra Fluoro Ethylene PTFE) processes by teflon.
Compared with prior art, a kind of liquid-phase chromatographic column of the present invention, the through hole edge of the contacted side of pressure cap and column jecket are provided with a circle groove, are provided with a circle packing washer in the groove.After pressure cap and joint were spirally connected, the ring-type post jamb of column jecket closely contacted with packing washer in the pressure cap groove.Thereby improved the sealing of chromatographic column, prolonged chromatographic column serviceable life.
Description of drawings
Accompanying drawing is used to provide further understanding of the present invention, is used to explain the present invention with various embodiments of the present invention, but is not construed as limiting the invention.In the accompanying drawings:
The medial surface that Fig. 1 joins for a kind of liquid-phase chromatographic column pressure cap and column jecket is provided with one and encloses groove and the A-A cut-open view of packing washer is not installed.
The medial surface that Fig. 2 joins for a kind of liquid-phase chromatographic column pressure cap and column jecket is provided with one and encloses groove and the A-A cut-open view of a packing washer has been installed.
Embodiment
Below in conjunction with accompanying drawing the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for explanation and explains the present invention, and be not used in qualification the present invention.
A-A cut-open view referring to figs. 1 through a kind of liquid-phase chromatographic column in the embodiment of the invention shown in 2.The chromatography separation principle is: be dissolved in when each component is through stationary phase in the moving phase (Mobile Phase); Because with the have an effect size, strong and weak different of (absorption, distribution, ion attraction, exclusion, affine) of stationary phase (Stationary Phase); The difference of hold-up time in stationary phase, thus successively from stationary phase, flow out.Liquid chromatography send out the incipient stage be with large diameter glass tube column under normal temperature and normal pressure with the liquid level difference transport flow mutually, claim " classical liquid phase chromatography ", the method inferior separating effect, length consuming time again.People have begun the method for research and utilization drive of high-pressure gas moving phase for this reason, claim again high performance liquid chromatography (High performance Liquid Chromatography, HPLC).Utilize this method carry out separation component efficient, fast, resolution is high, highly sensitive, so be called " MLC " again.Adopt the instrument of this kind technology just to be called " high performance liquid chromatograph ", its most important parts are exactly separating column.Separating column is generally processed by stainless steel; Inner wall smooth; Cavity is wherein filled out and can be filled various fillers (stationary phase); Utilize different fillers that different component is had the absorption of specificity property, distribution, ion attraction, exclusion, affine effect, (mainstream technology adopts suction-operated at present) makes the different component difference of the hold-up time in stationary phase, thereby successively from stationary phase, flows out.And detect various components through detecting device and be cut into branch and the time of flowing out separating column really, thereby reach the purpose of separations, detection.
In the present embodiment, a kind of liquid-phase chromatographic column, it comprises column jecket 3, pressure cap 1, joint 2, solid-phase adsorbent 6, sieve plate 8, two ends pressure cap 1 is provided with a circle groove 10 with through hole 7 edges of a medial surface of column jecket 3 bolts.Be provided with a sieve plate 8 in the middle of the groove 10 of pressure cap 1.The groove width 10a of the groove 10 of pressure cap 1 is greater than the wall thickness 3a of column jecket 3 arbitrary end end column jeckets.Be provided with a packing washer 4 in the groove 10 of pressure cap 1.Be provided with an annular projection portion 9 near the end at column jecket 3 two ends, the diameter of annular projection portion 9 is greater than the aperture of the minimum of joint 2.The stroke of joint 2 internal thread 2a is greater than the stroke of pressure cap 1 external thread 1a.The external thread 1a of pressure cap 1 and the internal thread 2a of joint 2 reliably are spirally connected, and the ring-type post jamb 3a of column jecket 2 and the groove of pressure cap 1 10 are chimeric and closely contact with packing washer 4.Packing washer 4 can (Poly Tetra Fluoro Ethylene PTFE) processes by teflon.Teflon just has good weatherability, can resist the corrosion of most of chemical substance, and have good resistance to ag(e)ing, is the ideal material of making the chromatographic column O-ring seal.
In this embodiment, the through hole edge of the contacted side of pressure cap and column jecket is provided with a circle groove.After pressure cap and joint were spirally connected, a packing washer that is provided with in the ring-type post jamb of column jecket and the pressure cap groove closely contacted, thereby had improved the sealing of liquid-phase chromatographic column, had prolonged the serviceable life of liquid-phase chromatographic column.
Be to be understood that; Though this instructions is described according to embodiment; But be not that each embodiment only comprises an independently technical scheme, this narrating mode of instructions only is for clarity sake, and those skilled in the art should make instructions as a whole; Technical scheme among each embodiment also can form other embodiments that it will be appreciated by those skilled in the art that through appropriate combination.
The listed a series of detailed description of preceding text only is specifying to feasibility embodiment of the present invention; They are not in order to restriction protection scope of the present invention, allly do not break away from equivalent embodiment or the change that skill of the present invention spirit done and all should be included within protection scope of the present invention.
Claims (9)
1. liquid-phase chromatographic column comprises: column jecket, pressure cap, joint, chromatograph packing material, sieve plate,
It is characterized in that the through hole edge of the medial surface that said pressure cap and column jecket join is provided with a circle groove.
2. chromatographic column according to claim 1 is characterized in that: be provided with a sieve plate in the middle of the groove of said pressure cap.
3. chromatographic column according to claim 1 is characterized in that: the groove width of the groove of said pressure cap is greater than the wall thickness of the arbitrary end of column jecket end column jecket.
4. chromatographic column according to claim 1 is characterized in that: be provided with packing washer in the groove of said pressure cap.
5. chromatographic column according to claim 1 is characterized in that: be provided with an annular projection portion near the end at said column jecket two ends, the maximum outside diameter of annular projection portion is greater than the aperture of the minimum of joint.
6. chromatographic column according to claim 1 is characterized in that said joint has internal thread, and pressure cap has external thread, and both pitch equate.
7. chromatographic column according to claim 1 is characterized in that: the stroke of said joint internal thread is greater than the externally threaded stroke of pressure cap.
8. chromatographic column according to claim 1 is characterized in that: the external thread of said pressure cap and the internal thread of joint reliably are spirally connected, and the ring-type post jamb of column jecket closely contacts with packing washer in the pressure cap groove.
9. chromatographic column according to claim 1 is characterized in that: said packing washer can (Poly Tetra Fluoro Ethylene PTFE) processes by teflon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101659953A CN102841166A (en) | 2011-06-20 | 2011-06-20 | Liquid chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101659953A CN102841166A (en) | 2011-06-20 | 2011-06-20 | Liquid chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102841166A true CN102841166A (en) | 2012-12-26 |
Family
ID=47368719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101659953A Pending CN102841166A (en) | 2011-06-20 | 2011-06-20 | Liquid chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102841166A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108799656A (en) * | 2018-09-03 | 2018-11-13 | 大连技嘉科技有限公司 | The pipe fitting that liquid chromatograph is rotated with anti-connecting tube |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1103025A (en) * | 1993-05-13 | 1995-05-31 | 武尔康洛克林公司 | Movable sleeve connector for plastic tube |
| EP1143243A1 (en) * | 2000-04-06 | 2001-10-10 | Bitec GmbH | Chromatography column |
| CN2553381Y (en) * | 2002-07-24 | 2003-05-28 | 中国科学院大连化学物理研究所 | High efficient liquid phase chromatographic column |
| CN2815464Y (en) * | 2005-08-27 | 2006-09-13 | 王柯 | Liquid-phase chromatographic fractionation column |
| CN2911683Y (en) * | 2006-07-11 | 2007-06-13 | 北京丰杰华信科学仪器有限公司 | Gel chromatographic column |
| CN201137778Y (en) * | 2007-06-06 | 2008-10-22 | 中国科学院大连化学物理研究所 | Sealing joint for connecting hose |
| CN201827564U (en) * | 2010-10-28 | 2011-05-11 | 浙江江山化工股份有限公司 | Quick gas cylinder charging connector |
| CN202141703U (en) * | 2011-06-20 | 2012-02-08 | 苏州赛分科技有限公司 | Liquid phase chromatographic column |
-
2011
- 2011-06-20 CN CN2011101659953A patent/CN102841166A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1103025A (en) * | 1993-05-13 | 1995-05-31 | 武尔康洛克林公司 | Movable sleeve connector for plastic tube |
| EP1143243A1 (en) * | 2000-04-06 | 2001-10-10 | Bitec GmbH | Chromatography column |
| CN2553381Y (en) * | 2002-07-24 | 2003-05-28 | 中国科学院大连化学物理研究所 | High efficient liquid phase chromatographic column |
| CN2815464Y (en) * | 2005-08-27 | 2006-09-13 | 王柯 | Liquid-phase chromatographic fractionation column |
| CN2911683Y (en) * | 2006-07-11 | 2007-06-13 | 北京丰杰华信科学仪器有限公司 | Gel chromatographic column |
| CN201137778Y (en) * | 2007-06-06 | 2008-10-22 | 中国科学院大连化学物理研究所 | Sealing joint for connecting hose |
| CN201827564U (en) * | 2010-10-28 | 2011-05-11 | 浙江江山化工股份有限公司 | Quick gas cylinder charging connector |
| CN202141703U (en) * | 2011-06-20 | 2012-02-08 | 苏州赛分科技有限公司 | Liquid phase chromatographic column |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108799656A (en) * | 2018-09-03 | 2018-11-13 | 大连技嘉科技有限公司 | The pipe fitting that liquid chromatograph is rotated with anti-connecting tube |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5868977B2 (en) | Biocompatible tubes for liquid chromatography systems | |
| JP6767973B2 (en) | Surface seal fluid connection system | |
| JP6257097B2 (en) | Torque limit connection | |
| US10422452B2 (en) | High pressure fluidic connection assemblies | |
| US8696038B2 (en) | Flat bottom fitting assembly | |
| WO2015122253A1 (en) | Flow path switching valve and liquid chromatographic device using such valve | |
| KR20080006532A (en) | Deaerator | |
| US9816647B2 (en) | Sealing element for a fluidic connection | |
| CN202141703U (en) | Liquid phase chromatographic column | |
| CN202145204U (en) | Liquid phase chromatographic column | |
| WO2019186223A1 (en) | Fluid mixer, pressure sensor | |
| CN202141702U (en) | Liquid phase chromatographic column | |
| CN102841166A (en) | Liquid chromatographic column | |
| US20110186497A1 (en) | Sealing configuration with metal-coated structure | |
| US8449658B2 (en) | High-pressure capillary degas assembly | |
| CN2911683Y (en) | Gel chromatographic column | |
| CN102841165A (en) | Liquid chromatographic column | |
| CN102052489A (en) | Valve for use in high-performance liquid chromatography having a spherical seat with beveled outer faces | |
| CN102841168A (en) | Liquid chromatographic column | |
| WO2014014622A1 (en) | High pressure fitting for supercritical fluid chromatography | |
| US20120132794A1 (en) | Apparatus And Components Thereof For Liquid Chromatography | |
| CN102841167A (en) | Liquid chromatographic column | |
| CN202141704U (en) | Liquid phase adsorption chromatograph separating column | |
| CN204359762U (en) | The syndeton of preparative scale chromatography post and preparative scale chromatography post | |
| CN117463001A (en) | Liquid chromatographic column |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121226 |