CN102841167A - Liquid chromatographic column - Google Patents
Liquid chromatographic column Download PDFInfo
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- CN102841167A CN102841167A CN2011101659968A CN201110165996A CN102841167A CN 102841167 A CN102841167 A CN 102841167A CN 2011101659968 A CN2011101659968 A CN 2011101659968A CN 201110165996 A CN201110165996 A CN 201110165996A CN 102841167 A CN102841167 A CN 102841167A
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Abstract
The invention discloses a liquid chromatographic column, comprising a support which is arranged on the inner wall of a column tube and is used for bearing a movable sieve plate; and the movable sieve plate which is disposed on the sieve plate support arranged on the inner wall of the column tube and is used for bearing filling materials. Different filling materials are filled in the two sides of the sieve plate. By using the structure, the effect of detecting and analyzing two components at the same time in one detection operation can be realized, thereby reducing the number of detection and the cost of detection, and improving the detection speed and detection efficiency.
Description
Technical field
The present invention relates to separation, the analysis technical field of chemicals, relate in particular to a kind of liquid phase adsorption chromatography column.
Background technology
The liquid phase adsorption chromatographic separation technology is a kind of dynamical Physical Separation Technology, and its core component is a chromatography column.Its separation principle is: the blending ingredients sample is dissolved in a kind of moving phase; Moving phase is under the condition of bigger pressure; Flow through the chromatograph packing material (filler) of can in chromatography column with certain flow velocity, because chromatograph packing material is to the difference of the absorption affinity of different component, so different component will flow through chromatograph packing material with different flow rate; And detected components flows out in the detecting device of liquid chromatograph time and components contents, separate and the purpose that detects thereby reach.Chromatographic column is general to adopt high-grade stainless steel to process its inner wall smooth.The effect of sieve plate is to catch solid-phase adsorbent, prevents from separating column, to flow out the Interference Detection effect.
But in liquid phase adsorption chromatography column commonly used; Generally in separating column, only fill a kind of solid-phase adsorbent usually; Because specific solid-phase adsorbent has specific adsorption to specific chemical, the separating column of this structure once can only separate, the material of a certain molecular diameter or chemical property in the test sample.Yet in actual mechanical process, the component of the material that some is to be detected is more complicated often, will carry out separating for several times, analysis to multiple sample for detecting its component, and this will cause testing process length consuming time, and it is high to detect cost.Therefore, be necessary existing liquid phase adsorption chromatography column is further improved, detect number of times, reduce and detect cost, improve the purpose of detection speed to realize improving reducing.
Summary of the invention
The object of the present invention is to provide a kind of liquid phase adsorption chromatography column, it can be realized improving reducing and detect number of times, reduces and detects cost, improves detection speed.
For realizing above-mentioned purpose, the invention provides a kind of liquid phase adsorption chromatography column, it comprises support, it is arranged at the inwall of column jecket, in order to carry active sieve plate; Active sieve plate, it is arranged on the sieve plate support of inwall of column jecket, in order to carry filler.
As further improvement of the present invention, said active sieve plate both sides fill can not absorption property filler.
As further improvement of the present invention, said column cap and column jecket are spirally connected, and can separate with the column jecket activity.
As further improvement of the present invention, respectively be provided with a fixed screen deck on the internal face of the side that the column cap at said column jecket two ends and column jecket are spirally connected, be used for fixing the filler in the chromatography column.
As further improvement of the present invention, the internal diameter of said column jecket is greater than the external diameter of active sieve plate.
As further improvement of the present invention, said fixed screen deck and active sieve plate are circular.
Compared with prior art, a kind of liquid phase adsorption chromatography column of the present invention is employed on the inwall of column jecket support is set, and active sieve plate is set to carry second kind of filler on the support.Detected sample is with after organic solvent mixes; High pressure (135～185kgf) drive under; Get into the liquid chromatography separating column from the separating column inlet; And through two kinds of different fillers, according to different fillers the absorption of different component in the sample is had specific principle, thereby be implemented in the effect of the plurality of target component in the disposable sample separation in the same liquid chromatography separating column.This simple in structure, be easy in same liquid phase adsorption chromatography column, add two kinds of solid-phase adsorbents, active sieve plate also is easy to take out, installs and cleans simultaneously, detects number of times to realize improving reducing, and reduces and detects cost, improves the purpose of detection speed.
Description of drawings
Accompanying drawing is used to provide further understanding of the present invention, is used to explain the present invention with various embodiments of the present invention, but is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the A-A cut-open view of not filling the liquid phase adsorption chromatography column of filler.
Fig. 2 is for filling the liquid phase adsorption chromatography column A-A cut-open view of first kind of filler.
Fig. 3 is for filling the liquid phase adsorption chromatography column A-A cut-open view of second kind of filler.
Embodiment
Below in conjunction with accompanying drawing the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for explanation and explains the present invention, and be not used in qualification the present invention.
A-A cut-open view referring to figs. 1 through a kind of liquid chromatography separating column in the embodiment of the invention shown in 3.The chromatography separation principle is: be dissolved in when each component is through stationary phase in the moving phase (Mobile Phase); Because with the have an effect size, strong and weak different of (absorption, distribution, ion attraction, exclusion, affine) of stationary phase (Stationary Phase); The difference of hold-up time in stationary phase, thus successively from stationary phase, flow out.Liquid chromatography send out the incipient stage be with large diameter glass tube column under normal temperature and normal pressure with the liquid level difference transport flow mutually, claim " classical liquid phase chromatography ", the method inferior separating effect, length consuming time again.People have begun the method for research and utilization drive of high-pressure gas moving phase for this reason, claim again high performance liquid chromatography (High performance Liquid Chromatography, HPLC).Utilize this method carry out separation component efficient, fast, resolution is high, highly sensitive, so be called " MLC " again.Adopt the instrument of this kind technology just to be called " high performance liquid chromatograph ", its most important parts are exactly separating column.Separating column is generally processed by high-grade stainless steel; Inner wall smooth; Cavity is wherein filled out and can be filled various fillers (stationary phase); Utilize different fillers that different component is had the absorption of specificity property, distribution, ion attraction, exclusion, affine effect, (mainstream technology adopts suction-operated at present) makes the different component difference of the hold-up time in stationary phase, thereby successively from stationary phase, flows out.And detect various components through detecting device and be cut into branch and the time of flowing out separating column really, thereby the purpose that reaches separations, detection, writes down.
In the present embodiment, a kind of liquid chromatography separating column comprises sample inlet column cap 60, column jecket 3, support 4, active sieve plate 21, fixed screen deck 20, sample export column cap 11.Adopt the mode secure fixation that is spirally connected guaranteeing sealing between sample export column cap 11 and the column jecket 3, and can be from movable separation the on the column jecket 3.Sample export column cap 11 and column jecket 3 are spirally connected and are provided with a fixed screen deck 20 in the middle of part inboard, in order to carry first kind of filler 5a in the separating column.The port that links to each other with column jecket 3 from sample inlet column cap 60 is then put into the height of column jecket 3 to support 4 with first filler, and is promptly shown in Figure 2.An active sieve plate 21 is set on the support in column jecket 34 then, in column jecket 3, fills the second filler 5b then near sample inlet joint 60 and column jecket 3.Adopt the mode secure fixation that is spirally connected to guarantee sealing sample inlet joint 60 and column jecket 3 at last, promptly shown in Figure 3.When the aperture 62 of the sample that contains two kinds of components from sample inlet column cap 60 flows into the column jecket 3, at first through the second filler 5b, because the wherein a certain middle component property of the second filler 5b specific adsorption; When sample continues to flow through the second filler 5a, the another kind of component of the second filler 5a specific adsorption.Because the difference of the absorption property of two kinds of components in different fillers also can be variant so flow out the time of chromatographic column, and finally in detecting device, be detected concrete component concentration and the time peak value that flows out chromatographic column.
In this embodiment, active sieve plate 21 carries the second filler 5b and the first filler 5a respectively with fixed screen deck 20.Can be in same separating column two kinds of components of disposable separation, detect number of times thereby reach to improve to reduce, reduce and detect cost, improve the purpose of detection speed.
Be to be understood that; Though this instructions is described according to embodiment; But be not that each embodiment only comprises an independently technical scheme, this narrating mode of instructions only is for clarity sake, and those skilled in the art should make instructions as a whole; Technical scheme among each embodiment also can form other embodiments that it will be appreciated by those skilled in the art that through appropriate combination.
The listed a series of detailed description of preceding text only is specifying to feasibility embodiment of the present invention; They are not in order to restriction protection scope of the present invention, allly do not break away from equivalent embodiment or the change that skill of the present invention spirit done and all should be included within protection scope of the present invention.
Claims (6)
1. liquid phase adsorption chromatography column, comprising: column cap, column jecket, fixed screen deck, filler is characterized in that this chromatography column also comprises:
Support, it is arranged at the inwall of column jecket, in order to carry active sieve plate;
Active sieve plate, it is arranged on the sieve plate support of inwall of column jecket, in order to carry filler.
2. chromatography column according to claim 1 is characterized in that: said active sieve plate both sides fill can not absorption property filler.
3. chromatography column according to claim 1 is characterized in that: said column cap and column jecket are spirally connected, and can separate with the column jecket activity.
4. chromatography column according to claim 1 is characterized in that: respectively be provided with a fixed screen deck on the internal face of the side that the column cap at said column jecket two ends and column jecket are spirally connected, be used for fixing the filler in the chromatography column.
5. chromatography column according to claim 1 is characterized in that: the internal diameter of said column jecket is greater than the external diameter of active sieve plate.
6. chromatography column according to claim 1 is characterized in that: said fixed screen deck and active sieve plate are for circular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101659968A CN102841167A (en) | 2011-06-20 | 2011-06-20 | Liquid chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101659968A CN102841167A (en) | 2011-06-20 | 2011-06-20 | Liquid chromatographic column |
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| CN102841167A true CN102841167A (en) | 2012-12-26 |
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| CN2011101659968A Pending CN102841167A (en) | 2011-06-20 | 2011-06-20 | Liquid chromatographic column |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208445A (en) * | 2019-07-09 | 2019-09-06 | 常州三泰科技有限公司 | Liquid-phase chromatographic column column tube and liquid-phase chromatographic column |
| CN110441408A (en) * | 2018-05-04 | 2019-11-12 | 研能科技股份有限公司 | Gas chromatography equipment |
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| US20040105782A1 (en) * | 1998-06-24 | 2004-06-03 | Shuqi Chen | Multi-layer testing column |
| CN1715907A (en) * | 2004-07-02 | 2006-01-04 | 中国科学院兰州化学物理研究所 | The gas chromatography analysis method of micro ethanol in the blood of human body |
| EP1734363A1 (en) * | 2004-03-31 | 2006-12-20 | Saika Tech. Institute Foundation | Stationary phase extraction cartridge |
| CN101413932A (en) * | 2007-10-17 | 2009-04-22 | 中国科学院大连化学物理研究所 | Two-phase integral column as well as preparation and application thereof |
| CN201532386U (en) * | 2009-10-13 | 2010-07-21 | 苏州汇通色谱分离纯化有限公司 | Two section type taper liquid chromatography preparation post |
| CN101180113B (en) * | 2005-05-23 | 2011-04-06 | 比肖夫分析技术与仪器有限责任公司 | Elements for separating substances by distributing between a stationary and a mobile phase, and method for the production of a separating device |
| CN202141704U (en) * | 2011-06-20 | 2012-02-08 | 苏州赛分科技有限公司 | Liquid phase adsorption chromatograph separating column |
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2011
- 2011-06-20 CN CN2011101659968A patent/CN102841167A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040105782A1 (en) * | 1998-06-24 | 2004-06-03 | Shuqi Chen | Multi-layer testing column |
| EP1734363A1 (en) * | 2004-03-31 | 2006-12-20 | Saika Tech. Institute Foundation | Stationary phase extraction cartridge |
| CN1715907A (en) * | 2004-07-02 | 2006-01-04 | 中国科学院兰州化学物理研究所 | The gas chromatography analysis method of micro ethanol in the blood of human body |
| CN101180113B (en) * | 2005-05-23 | 2011-04-06 | 比肖夫分析技术与仪器有限责任公司 | Elements for separating substances by distributing between a stationary and a mobile phase, and method for the production of a separating device |
| CN101413932A (en) * | 2007-10-17 | 2009-04-22 | 中国科学院大连化学物理研究所 | Two-phase integral column as well as preparation and application thereof |
| CN201532386U (en) * | 2009-10-13 | 2010-07-21 | 苏州汇通色谱分离纯化有限公司 | Two section type taper liquid chromatography preparation post |
| CN202141704U (en) * | 2011-06-20 | 2012-02-08 | 苏州赛分科技有限公司 | Liquid phase adsorption chromatograph separating column |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441408A (en) * | 2018-05-04 | 2019-11-12 | 研能科技股份有限公司 | Gas chromatography equipment |
| CN110208445A (en) * | 2019-07-09 | 2019-09-06 | 常州三泰科技有限公司 | Liquid-phase chromatographic column column tube and liquid-phase chromatographic column |
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Application publication date: 20121226 |