CN110208445A - Liquid-phase chromatographic column column tube and liquid-phase chromatographic column - Google Patents
Liquid-phase chromatographic column column tube and liquid-phase chromatographic column Download PDFInfo
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- CN110208445A CN110208445A CN201910613691.5A CN201910613691A CN110208445A CN 110208445 A CN110208445 A CN 110208445A CN 201910613691 A CN201910613691 A CN 201910613691A CN 110208445 A CN110208445 A CN 110208445A
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- chromatographic column
- phase chromatographic
- sieve plate
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- 239000007791 liquid phase Substances 0.000 title claims abstract description 86
- 230000002093 peripheral effect Effects 0.000 claims abstract description 11
- 230000003014 reinforcing effect Effects 0.000 claims description 18
- 230000005526 G1 to G0 transition Effects 0.000 claims description 12
- 230000007246 mechanism Effects 0.000 claims description 11
- 239000003463 adsorbent Substances 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 230000007704 transition Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 230000008602 contraction Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000006978 adaptation Effects 0.000 abstract description 2
- 238000005304 joining Methods 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 239000000306 component Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/606—Construction of the column body with fluid access or exit ports
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention discloses a kind of liquid-phase chromatographic column column tube and liquid-phase chromatographic column, liquid-phase chromatographic column column tube includes the cylinder of upper end opening, lower end closed;The cylinder has cylinder peripheral wall and lower end closed wall, the lower end closed wall and the cylinder peripheral wall surround barrel chamber, the lower end closed wall is equipped with liquid-phase outlet, and the lower end closed wall is additionally provided with radial dimension less than lower end closed wall, adaptation and the blind hole for accommodating lower sieve plate and being connected with liquid-phase outlet.The present invention can carry the lower sieve plate of smaller size, to reduce the contraction of lower sieve plate, its separating effect is improved, while the liquid-phase chromatographic column of plurality of specifications can be made using the lower sieve plate of same specification type, the use specification type for reducing lower sieve plate, in addition also reduces manufacturing cost.
Description
Technical field
The present invention relates to a kind of liquid-phase chromatographic column column tube and liquid-phase chromatographic columns.
Background technique
Currently, liquid chromatography technology is that one kind quickly prepares column chromatography format, it is based upon pump and generates pressure acceleration
Mobile phase passes through the elution speed of pre-filling type chromatography column, and core component is liquid-phase chromatographic column, and separation principle is: will
Blend sample is dissolved in a kind of mobile phase, adsorbs it by the filling stationary phase in liquid-phase chromatographic column with certain rate
Agent, it is since mixture each component is in the different solubility of stationary phase and mobile phase, i.e., different in two alternate distribution coefficients, therefore mix
Object component will flow through stationary phase adsorbent with different rates, to achieve the purpose that separation.The liquid-phase chromatographic column of the prior art
Structure in include lower sieve plate and column tube, lower sieve plate is placed directly within the lower end of the chamber of column tube, accepted using lower envelope wall or lower cover
It stays sieve plate, the liquid-phase chromatographic column of this structure has the disadvantage in that
1, lower sieve plate needs and the chamber of column tube is adapted to, and diameter is bigger, since the sieve plate shrinking percentage of identical material is one
Fixed, if its diameter is bigger, the contraction of sieve plate in actual use is more severe, this will cause the periphery meeting of the chamber of column tube
There are certain gaps with sieve plate, so that separating effect is bad, there is the phenomenon that leakage;
2, the liquid-phase chromatographic column of this structure, each structure need a kind of lower sieve plate of size to go to be adapted to, and use scope is not wide;
3, due to needing the lower sieve plate of larger size to go to be adapted to, higher cost.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of existing technologies, a kind of liquid-phase chromatographic column column tube is provided,
Its lower sieve plate that can carry smaller size improves its separating effect, while can make a variety of to reduce the contraction of lower sieve plate
The liquid-phase chromatographic column of specification uses the lower sieve plate of same specification type, reduces the use specification type of lower sieve plate, in addition also drops
Low manufacturing cost.
In order to solve the above-mentioned technical problem, the technical scheme is that a kind of liquid-phase chromatographic column column tube, it includes upper end
The cylinder of opening, lower end closed;The cylinder includes
Cylinder peripheral wall;
Lower end closed wall, the lower end closed wall and the cylinder peripheral wall surround barrel chamber, and the lower end closed wall goes out equipped with liquid phase
Mouthful, the lower end closed wall be additionally provided with radial dimension less than lower end closed wall, adaptation and accommodate lower sieve plate and with liquid-phase outlet phase
The blind hole of connection.
Further for the liquid phase by stationary phase adsorbent can be made to be guided on lower sieve plate well, the lower end seal
The joining place closed between the radial outer end of the upper surface of wall and the medial surface of the cylinder peripheral wall is arc transition shape.
Further for cylinder can be made to carry stationary phase adsorbent well, it is set to be evenly distributed, the lower end closed
Part of the upper surface of wall (12) in addition to blind hole is plane.
Further, the circle center of the blind hole is overlapped with the circle center of the liquid-phase outlet.
Further for liquid phase catchment effect is improved, the lower end surface of the blind hole, which is equipped with, leads the liquid phase after lower sieve plate
It flow to the liquid phase deflector of liquid-phase outlet.
Further, the liquid phase deflector includes:
The center deflector hole being arranged with the liquid-phase outlet with circle center;
It is connected with the center deflector hole and along at least diversion trench for radially extending setting of center deflector hole.
Further, the diversion trench is equipped at least two, and uniformly distributed along the circumferential direction of center deflector hole.
Further for the intensity for increasing lower end closed wall or even cylinder, the lower end surface of the lower end closed wall is equipped with strong
Spend reinforcing mechanisms;
Further, the intensity reinforcing mechanisms include at least one along the circumferentially extending of lower end closed wall circumferential reinforcing rib and/
Or at least one along lower end closed wall the radial reinforcing rib radially extended.
Further, the annular convex rib suitable for anti-solid powder powder leakage is provided on the lower end surface of the blind hole.
The present invention also provides a kind of liquid-phase chromatographic columns, it includes:
Liquid-phase chromatographic column column tube;
Lower sieve plate, the lower sieve plate are placed in the blind hole of the lower end closed wall of the liquid-phase chromatographic column column tube;
Lid, described to cover the upper end for being connected to the liquid-phase chromatographic column column tube middle cylinder body, the lid is formed after connecting with the cylinder
One accommodating cavity;
Built-in plug, the built-in plug are set in the accommodating cavity, and the built-in plug is equipped with fluid inlet, the fluid inlet
Partially extend through the lid;
The upper sieve plate of built-in plug lower section, the upper sieve plate and the lower sieve plate in accommodating cavity are placed in the accommodating cavity
Between area filling stationary phase adsorbent.
Further, described to cover the upper end for being threadingly attached to or being connected to by welding the cylinder.
After above-mentioned technical proposal, the present invention has the following beneficial effects:
1, compared with prior art, liquid-phase chromatographic column column tube of the invention can carry the lower sieve plate of smaller size, due to identical material
The sieve plate shrinking percentage of material is certain, if its diameter is smaller, the contraction of sieve plate in actual use is smaller, to reduce
The contraction of lower sieve plate, the periphery and blind hole for avoiding lower sieve plate generate biggish gap, to finally improve its separating effect;
2, after use further as lower sieve plate, contraction is small compared with the prior art, to improve the reuse rate of lower sieve plate;
3, compared with prior art, the present invention does not need a kind of a kind of corresponding lower sieve using specification type of liquid-phase chromatographic column of specification
Plate, it is only necessary to open up the blind hole of corresponding size on lower end closed wall, make it possible to the liquid-phase chromatographic column of plurality of specifications
Using the lower sieve plate of same specification type, reduce the use specification type of lower sieve plate.
4, compared with prior art, liquid-phase chromatographic column column tube of the invention carries the lower sieve plate of smaller size and reduces lower sieve
The use specification type of plate, reduces manufacturing cost.
Detailed description of the invention
Fig. 1 is the perspective view one of liquid-phase chromatographic column column tube of the invention;
Fig. 2 is the perspective view two of liquid-phase chromatographic column column tube of the invention;
Fig. 3 is the perspective view of liquid-phase chromatographic column of the invention;
Fig. 4 is the structure sectional view of liquid-phase chromatographic column of the invention;
Fig. 5 is the perspective view of the built-in plug of liquid-phase chromatographic column of the invention.
Specific embodiment
In order that the present invention can be more clearly and readily understood, right below according to specific embodiment and in conjunction with attached drawing
The present invention is described in further detail.
Embodiment one
As shown in Fig. 1,2,4, a kind of liquid-phase chromatographic column column tube, it includes the cylinder 1 of upper end opening, lower end closed;The cylinder
It includes
Cylinder peripheral wall 11;
Lower end closed wall 12, the lower end closed wall 12 and the cylinder peripheral wall 11 surround barrel chamber 10, on the lower end closed wall 12
Equipped with liquid-phase outlet 12a, the lower end closed wall 12 is additionally provided with radial dimension and is less than lower end closed wall 12, is adapted to and accommodates lower sieve
Plate 2 and the blind hole 12b being connected with liquid-phase outlet 12a.
In the present embodiment, lower sieve plate 2 is cylindrical structure, and the diameter of blind hole 12b is adapted to the diameter of lower sieve plate 2, and
And the depth of blind hole 12b is equal to or slightly less than the height of lower sieve plate 2, in this way primarily to being carried on lower end closed wall 12
Stationary phase adsorbent, and liquid phase can be made to flow on the lower sieve plate 2 in blind hole 12b.
Specifically, as shown in figure 4, the radial outer end of the upper surface 12c of the lower end closed wall 12 and the cylinder peripheral wall 11
Medial surface between joining place be arc transition shape, the arc transition shape setting joining place liquid phase can be guided very well downward
Sieve plate 2 flows, and the position A in Fig. 1 is joining place.
Specifically, as shown in Figure 1, making it be evenly distributed in order to make cylinder 1 carry stationary phase adsorbent well,
Part of the upper surface 12c of the lower end closed wall 12 in addition to blind hole 12b is plane.
Specifically, part of the upper surface 12c of the lower end closed wall 12 in addition to blind hole 12b can also set deflector, with
Liquid phase is guided at lower sieve plate 2 well.
Specifically, as shown in Figure 1, the circle center of the blind hole 12b and the circle center of liquid-phase outlet 12a weight
It closes.The design is primarily to can make liquid phase flow out well from liquid-phase outlet 12a.
As shown in Figure 1, the lower end surface of the blind hole 12b is equipped with will be after lower sieve plate 2 in order to improve liquid phase catchment effect
Liquid phase be guided to the liquid phase deflector of liquid-phase outlet 12a.
As shown in Fig. 2, the liquid phase deflector is such as, but not limited to flowering structure comprising:
The center deflector hole 12e being arranged with the liquid-phase outlet 12a with circle center;
It is connected with the center deflector hole 12e and along at least diversion trench for radially extending setting of center deflector hole 12e
12f。
The diversion trench 12f is equipped at least two, and uniformly distributed along the circumferential direction of center deflector hole 12e.
In the present embodiment, diversion trench 12f is equipped with 8, and but not limited to this.
As shown in Fig. 2, the lower end surface 12d of the lower end closed wall 12 is equipped with intensity reinforcing mechanisms;
The intensity reinforcing mechanisms include at least one along the circumferentially extending of lower end closed wall 12 circumferential reinforcing rib 12g and/or
At least one along lower end closed wall 12 the radial reinforcing rib 12h radially extended;In the present embodiment, the intensity reinforcing mechanisms
Long radial reinforcing rib 12h is adjusted including 2 circumferential direction reinforcing rib 12g and positioned at 12 short radial reinforcing rib 12h of inside and 8,
But not limited to this, can also only include circumferential reinforcing rib 12g or only include radial reinforcing rib 12h.
As shown in Figure 1, being provided with the annular convex rib 12m suitable for anti-solid powder powder leakage on the lower end surface of the blind hole 12b.
Embodiment two
As shown in Fig. 3 ~ 5, a kind of liquid-phase chromatographic column, it includes:
Liquid-phase chromatographic column column tube in embodiment one;
Lower sieve plate 2, in the blind hole 12b for the lower end closed wall 12 that the lower sieve plate 2 is placed in the liquid-phase chromatographic column column tube;
Lid 3, the lid 3 is connected to the upper end of the liquid-phase chromatographic column column tube middle cylinder body 1, after the lid 3 and the cylinder 1 connect
Form an accommodating cavity 20;
Built-in plug 4, the built-in plug 4 is set in the accommodating cavity 20, and the built-in plug 4 is equipped with fluid inlet 41, described
Fluid inlet 41 partially extends through the lid 3;
The upper sieve plate 5 of built-in 4 lower section of plug, the upper sieve plate 5 and institute in accommodating cavity 20 are placed in the accommodating cavity 20
State the area filling stationary phase adsorbent between lower sieve plate 2.
As shown in figure 5, in the present embodiment, the lower end surface of built-in plug 4 can be equipped with uniform divided flows mechanism 42, the uniform divided flows
Mechanism is primarily to even in liquid phase is distributed to upper sieve plate 5 and flows through stationary phase adsorbent very well, uniform divided flows mechanism 42 is
The customary means of those skilled in the art, the present invention, which is not done, specifically to be repeated.
In the present embodiment, liquid phase can enter from fluid inlet 41, and the shunting by uniform divided flows mechanism 42 flows through
Sieve plate 5 then flowed through stationary phase adsorbent, passes through lower sieve plate 2, finally goes out from liquid-phase outlet 12a.
The lid 3 can be threadingly attached to or be connected to by welding the upper end of the cylinder 1.
Particular embodiments described above, pair present invention solves the technical problem that, technical scheme and beneficial effects carry out
It is further described, it should be understood that the above is only a specific embodiment of the present invention, is not limited to this
Invention, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this hair
Within bright protection scope.
In the description of the present invention, it is to be understood that, indicating position or the term of positional relationship are based on shown in attached drawing
Orientation or positional relationship, be merely for convenience of description of the present invention and simplification of the description, rather than the equipment of indication or suggestion meaning
Or element must have a particular orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral;It can be mechanical connect
It connects, is also possible to be electrically connected;It can be directly connected, can also can be in two elements indirectly connected through an intermediary
The interaction relationship of the connection in portion or two elements.It for the ordinary skill in the art, can be according to specific feelings
Condition understands the concrete meaning of above-mentioned term in the present invention.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, or be somebody's turn to do
Invention product using when the orientation or positional relationship usually put, be merely for convenience of description of the present invention and simplification of the description, without
It is that the device of indication or suggestion meaning or element must have a particular orientation, be constructed and operated in a specific orientation, therefore not
It can be interpreted as limitation of the present invention.In addition, term " first ", " second ", " third " etc. are only used for distinguishing description, and cannot manage
Solution is indication or suggestion relative importance.
In addition, the terms such as term "horizontal", "vertical", " pendency " are not offered as requiring component abswolute level or pendency, and
It is that can be slightly tilted.It is not to indicate the structure if "horizontal" only refers to that its direction is more horizontal with respect to for "vertical"
It has to fully horizontally, but can be slightly tilted.
In the present invention unless specifically defined or limited otherwise, fisrt feature can be on or below second feature
Directly contacted including the first and second features, also may include the first and second features be not direct contact but by them it
Between other characterisation contact.Moreover, fisrt feature is on second feature, top and above include fisrt feature second spy
Right above sign and oblique upper, or first feature horizontal height is merely representative of higher than second feature.Fisrt feature second feature it
Under, lower section and fisrt feature included below be directly below and diagonally below the second feature, or be merely representative of first feature horizontal height
Less than second feature.
Claims (10)
1. a kind of liquid-phase chromatographic column column tube, which is characterized in that it includes the cylinder (1) of upper end opening, lower end closed;The cylinder
It includes
Cylinder peripheral wall (11);
Lower end closed wall (12), the lower end closed wall (12) and the cylinder peripheral wall (11) surround barrel chamber (10), the lower end seal
Wall (12) are closed equipped with liquid-phase outlet (12a), the lower end closed wall (12) is additionally provided with radial dimension less than lower end closed wall
(12), the blind hole (12b) for being adapted to and accommodating lower sieve plate (2) and be connected with liquid-phase outlet (12a).
2. liquid-phase chromatographic column column tube according to claim 1, which is characterized in that
Rank between the radial outer end of the upper surface (12c) of the lower end closed wall (12) and the medial surface of the cylinder peripheral wall (11)
Meeting place is arc transition shape.
3. liquid-phase chromatographic column column tube according to claim 1, which is characterized in that
Part of the upper surface (12c) of the lower end closed wall (12) in addition to blind hole (12b) is plane.
4. liquid-phase chromatographic column column tube according to claim 1, which is characterized in that
The circle center of the blind hole (12b) is overlapped with the circle center of the liquid-phase outlet (12a).
5. liquid-phase chromatographic column column tube according to claim 1, which is characterized in that
The lower end surface of the blind hole (12b) is equipped with the liquid phase that the liquid phase after lower sieve plate (2) is guided to liquid-phase outlet (12a)
Deflector.
6. liquid-phase chromatographic column column tube according to claim 5, which is characterized in that the liquid phase deflector includes:
The center deflector hole (12e) being arranged with the liquid-phase outlet (12a) with circle center;
It is connected with the center deflector hole (12e) and along at least water conservancy diversion for radially extending setting of center deflector hole (12e)
Slot (12f).
7. liquid-phase chromatographic column column tube according to claim 1, which is characterized in that
The lower end surface (12d) of the lower end closed wall (12) is equipped with intensity reinforcing mechanisms;
The intensity reinforcing mechanisms include at least one along the circumferentially extending of lower end closed wall (12) circumferential reinforcing rib (12g)
And/or at least one along lower end closed wall (12) the radial reinforcing rib (12h) radially extended.
8. liquid-phase chromatographic column column tube according to claim 1, which is characterized in that
The annular convex rib (12m) suitable for anti-solid powder powder leakage is provided on the lower end surface of the blind hole (12b).
9. a kind of liquid-phase chromatographic column, which is characterized in that it includes:
Such as liquid-phase chromatographic column column tube described in any item of the claim 1 to 8;
Lower sieve plate (2), the lower sieve plate (2) are placed in the blind hole of the lower end closed wall (12) of the liquid-phase chromatographic column column tube
In (12b);
It covers (3), the lid (3) is connected to the upper end of the liquid-phase chromatographic column column tube middle cylinder body (1), lid (3) and the cylinder
An accommodating cavity (20) are formed after body (1) connection;
Built-in plug (4), the built-in plug (4) are set in the accommodating cavity (20), and the built-in plug (4) is equipped with fluid inlet
(41), the fluid inlet (41) partially extends through the lid (3);
The upper sieve plate (5) being placed in the accommodating cavity (20) below built-in plug (4), the upper sieve in accommodating cavity (20)
Area filling stationary phase adsorbent between plate (5) and the lower sieve plate (2).
10. liquid-phase chromatographic column according to claim 9, which is characterized in that
The lid (3) is connected through a screw thread or is connected to by welding the upper end of the cylinder (1).
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CN201910613691.5A CN110208445A (en) | 2019-07-09 | 2019-07-09 | Liquid-phase chromatographic column column tube and liquid-phase chromatographic column |
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CN201910613691.5A CN110208445A (en) | 2019-07-09 | 2019-07-09 | Liquid-phase chromatographic column column tube and liquid-phase chromatographic column |
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CN205815185U (en) * | 2016-07-27 | 2016-12-21 | 中国水产科学研究院黑龙江水产研究所 | A kind of liquid chromatography separation column |
CN206192956U (en) * | 2016-11-21 | 2017-05-24 | 天津农学院 | Drug residue detects and uses extraction device |
CN106730998A (en) * | 2017-02-22 | 2017-05-31 | 周鑫 | A kind of distributor in liquid-phase chromatographic column |
CN206804604U (en) * | 2017-03-30 | 2017-12-26 | 南通裕弘分析仪器有限公司 | A kind of performance liquid chromatographic column |
CN206837552U (en) * | 2017-06-16 | 2018-01-05 | 常州三泰科技有限公司 | Liquid chromatography separation column |
CN207096191U (en) * | 2017-07-10 | 2018-03-13 | 南通裕弘分析仪器有限公司 | A kind of performance liquid chromatographic column |
CN109100203A (en) * | 2018-08-22 | 2018-12-28 | 佛山科学技术学院 | A kind of solid-phase extraction column for residue of veterinary drug measurement |
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