CN101947392A

CN101947392A - Shunt plate for preparing chromatographic column

Info

Publication number: CN101947392A
Application number: CN 201010254274
Authority: CN
Inventors: 周胜; 徐慧
Original assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Current assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Priority date: 2010-08-13
Filing date: 2010-08-13
Publication date: 2011-01-19
Anticipated expiration: 2030-08-13
Also published as: CN101947392B

Abstract

The invention relates to a shunt plate for preparing a chromatographic column. The shun plate consists of an upper layer, a middle shunt layer and a bottom layer; upper and lower corresponding screw holes are formed in the upper layer and the middle shunt layer of the shunt plate respectively; the upper layer is a thin column with the thickness of 1 to 20 millimeters, the front of the thin column is provided with a central shunt groove, the back of the thin column is at least provided with six equal branch-shaped shunt grooves, and the central shunt groove is communicated with the branch-shaped shunt grooves; the bottom layer is a thin column with the thickness of 1 to 20 millimeters, shunt holes are uniformly distributed on the bottom layer, and the bottom layer is provided with four sunk screw holes corresponding to the screw holes; and the diameters of the shunt holes beside the four sunk screw holes are 1.6 millimeters, and the diameter of the rest shunt holes is 0.6 to 5 times that of the shunt holes. The shunt plate has the advantages that: 1, the shunt effect is remarkable due to matched use of multiple channels, a screen and a porous plate; 2, the shunt plate can be processed in large batch, and has low processing difficulty and low cost; and 3, the shunt plate can be manufactured with various specifications and sizes, unit number of the shunt grooves, aperture size and density of the porous plate and wide application range.

Description

A kind of preparative chromatography post flow distribution plate

Technical field:

The present invention relates to a kind of preparative chromatography post flow distribution plate, be used for the interior liquid of preparative column and evenly shunt, it can Fast Installation clean, and is suitable for pressing among the overwhelming majority column separating purification of filler to prepare requirement.

Background technology:

The dividing technology of preparative chromatography post is the key technology of preparative chromatography, and especially in the medium-and-large-sized preparative chromatography post more than internal diameter 50mm, the fluid of flow distribution plate/sample distribution effects decision is by testing the linear feasibility of amplifying of technology.

The above medium-and-large-sized preparative chromatography post of internal diameter 50mm need be considered the uniform distribution of liquid in post, and fluid/sample allocation of parameters is the key that effective post separates, and is the linear key technology of amplifying of chromatogram.

Summary of the invention:

The present invention is directed to the use problem and the shortcoming of the medium-and-large-sized preparative chromatography post more than the internal diameter 50mm in the past, a kind of preparative chromatography post flow distribution plate is provided.

The technical solution adopted for the present invention to solve the technical problems is: a kind of preparative chromatography post flow distribution plate, by the upper strata, middle part shunting layer and three layers of composition of bottom, be respectively equipped with corresponding screw hole up and down thereon in four quadrant areas of layer and middle part shunting layer, described upper strata is the thick thin cylinder of 1mm～20mm, its front is provided with the center splitter box, the back side is provided with the branching shape splitter box of six five equilibriums at least, described center splitter box and branching shape splitter box communicate, described bottom is the thick thin cylinder of 1mm～20mm, be evenly distributed with tap hole on the described bottom, and on bottom, be provided with and corresponding four the sunk screw holes of screw hole, the tap hole on next door, described four sunk screw holes is that diameter is 1.6mm, and all the other tap hole diameters are 0.6～5 times of tap hole diameter on next door, four sunk screw holes.

Further:

The main flow conduit width dimensions of described branching shape splitter box is 0.6mm～5mm, 2/3 of its effluent conduit width main flow conduit width.

Described middle part shunting layer is welded into one by four layers of stainless steel filtering net point, and four layers of stainless steel filtering net of described middle part shunting layer mesh number number from top to bottom is respectively 20-325-60-20.

Described stainless steel filtering net is the 316L stainless steel.

The distribution mode of described tap hole is: the center of circle with bottom is that distribution center outwards disperses with the form of annulus, and the distance between its each annulus is 1.2mm～12mm, and the number of variations of the tap hole that distributes on the described annulus is 6*1-6*2-6*3...6*N.

The employed material of described upper strata and bottom is PTFE or PTFE modification or PVDF or PFA fluorine material.

The present invention is directed to the problem and the shortcoming of the medium-and-large-sized preparative chromatography post use more than the internal diameter 50mm in the past, design and manufacturing have solved following technical problem:

1, adopts the even velocity of flow of even separate system assurance from inlet tube to whole column section of particular design;

2, pass through the design of sunk screw hole and screw hole, but Fast Installation and disassembly, cleaning;

3, the material of flow distribution plate is PTFE/PTFE modification/PVDF/PFA fluorine material, and middle part shunting layer employing 316L stainless steel, and stable chemical performance can be used for all kinds solvent.

The present invention has compared following beneficial effect with existing shunting product:

1, being used of multichannel, screen cloth and porous plate, shunting effect is remarkable;

2, CNC processing, but Mould Machining in enormous quantities, difficulty of processing is little, and cost is low;

3, can customized one-tenth all size size, and the element number of shunting conduit, the pore size of porous plate and density, applied widely.

Description of drawings:

Fig. 1 is an overall structure schematic diagram of the present invention.

Fig. 2 is the upward view of Fig. 1.

Fig. 3 is the vertical view on upper strata of the present invention.

Fig. 4 is for being the upward view on upper strata of the present invention.

Fig. 5 is the vertical view of the present invention middle part shunting layer.

Fig. 6 is the vertical view of bottom of the present invention.

Fig. 7 is a work schematic diagram of the present invention.

Among the figure: 1, upper strata; 2, middle part shunting layer; 3, bottom; 4, center splitter box; 5, tap hole; 6, sunk screw hole; 7, main flow conduit; 8, effluent conduit; 9, screw hole.

The specific embodiment:

The present invention is further described below in conjunction with drawings and Examples.

A kind of preparative chromatography post flow distribution plate as shown in Figure 1, 2 is made up of for 3 three layers upper strata 1, middle part shunting layer 2 and bottom, is respectively equipped with corresponding up and down screw hole 9 in four quadrant areas of layer 1 and middle part shunting layer 2 thereon.Described upper strata 1 is the thick thin cylinder of 1mm～20mm, its material is PTFE or PTFE modification or PVDF or PFA fluorine material or other anti-solvent materials, its front is provided with center splitter box 4, the back side is provided with the branching shape splitter box of six five equilibriums at least, main flow conduit 7 width dimensions of described branching shape splitter box are 0.6mm～5mm, its effluent conduit width is 2/3 of main flow conduit 7 width, described center splitter box 4 and branching shape splitter box communicate (as Fig. 3,4); Described middle part shunting layer 2 is welded into one by four layers of stainless steel filtering net point, and its material is the 316L stainless steel, and its four layers of stainless steel filtering nets mesh number number from top to bottom is respectively 20-325-60-20 (as Fig. 5), or is other types sieve plate and filter membrane; Described bottom 3 is the thick thin cylinder of 1mm～20mm, its material is PTFE or PTFE modification or PVDF or PFA fluorine material or other anti-solvent materials, be evenly distributed with tap hole 5 on the described bottom 3, and on bottom, be respectively equipped with and screw hole 9 corresponding sunk screw holes 6, diameter at the tap hole 5 on 6 next doors, four sunk screw holes is 1.6mm, all the other tap hole 5 diameters are 0.6～5 times of diameter of the tap hole 5 on 6 next doors, four sunk screw holes, the distribution mode of described tap hole 5 is: the center of circle with bottom 3 is that distribution center outwards disperses with the form of annulus, distance between its each annulus is 1.2mm～12mm, and the number of variations of the tap hole 5 that distributes on the described annulus is 6*1-6*2-6*3...6*N (as Fig. 6).The present invention can mount and dismount fast, during installation, upper strata 1, middle part shunting layer 2 and bottom 3 are overlaped successively from top to bottom, fix with countersunk head screw then, and when the needs disassembly, cleaning, only need back out four countersunk head screws and three layers separately can be changed or clean.

In conjunction with as shown in Figure 7, during concrete the use, so the solvent at first center splitter box 4 by upper strata 1 branches in the main flow conduit 7 of branching shape splitter box of its bottom surface, diffuse to effluent conduit 8 simultaneously, make its fast all and branch to the second layer 2, by its four layers of stainless steel filtering nets its refinement is branched to the 3rd layer 3 then, carry out refinement again by the tap hole 5 on the 3rd layer 3 at last and branch in the preparative chromatography post.

It is emphasized that: above only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, every foundation technical spirit of the present invention all still belongs in the scope of technical solution of the present invention any simple modification, equivalent variations and modification that above embodiment did.

Claims

1. preparative chromatography post flow distribution plate, it is characterized in that: described preparative chromatography post flow distribution plate is by upper strata (1), middle part shunting layer (2) and (3) three layers of composition of bottom, be respectively equipped with corresponding screw hole (9) up and down thereon in four quadrant areas of layer (1) and middle part shunting layer (2), described upper strata (1) is the thick thin cylinder of 1mm～20mm, its front is provided with center splitter box (4), the back side is provided with the branching shape splitter box of six five equilibriums at least, described center splitter box (4) and branching shape splitter box communicate, described bottom (3) is the thick thin cylinder of 1mm～20mm, be evenly distributed with tap hole (5) on the described bottom (3), and on bottom, be provided with and corresponding four the sunk screw holes of screw hole (9) (6), the tap hole (5) on described four sunk screw holes (6) next doors is 1.6mm for diameter, and all the other tap holes (5) diameter is 0.6～5 times of tap hole (5) diameter on next door, four sunk screw holes (6).

2. preparative chromatography post flow distribution plate according to claim 1 is characterized in that: main flow conduit (7) width dimensions of described branching shape splitter box is 0.6mm～5mm, 2/3 of its effluent conduit (8) width main flow conduit (7) width.

3. preparative chromatography post flow distribution plate according to claim 1, it is characterized in that: described middle part shunting layer (2) is welded into one by four layers of stainless steel filtering net point, and four layers of stainless steel filtering net of described middle part shunting layer (2) mesh number number from top to bottom is respectively 20-325-60-20.

4. preparative chromatography post flow distribution plate according to claim 3 is characterized in that: described stainless steel filtering net is the 316L stainless steel.

5. preparative chromatography post flow distribution plate according to claim 1, it is characterized in that: the distribution mode of described tap hole (5) is: the center of circle with bottom (3) is that distribution center outwards disperses with the form of annulus, distance between its each annulus is 1.2mm～12mm, and the number of variations of the tap hole that distributes on the described annulus (5) is 6*1-6*2-6*3...6*N.

6. preparative chromatography post flow distribution plate according to claim 1 is characterized in that: described upper strata (1) and the employed material of bottom (3) are PTFE or PTFE modification or PVDF or PFA fluorine material.