CN219084852U

CN219084852U - Liquid chromatographic column

Info

Publication number: CN219084852U
Application number: CN202223581875.4U
Authority: CN
Inventors: 曹悦兴; 张展
Original assignee: Shandong Shangcheng Quality Inspection Co ltd
Current assignee: Shandong Shangcheng Quality Inspection Co ltd
Priority date: 2022-12-31
Filing date: 2022-12-31
Publication date: 2023-05-26
Anticipated expiration: 2032-12-31

Abstract

The utility model belongs to the technical field of liquid chromatography, and particularly relates to a liquid chromatography column. The liquid chromatographic column comprises a column tube, column caps are fixedly arranged at the upper end and the lower end of the column tube, a push plate is slidably arranged in the column cap, a compression spring is fixedly arranged on the top surface of the push plate, the other end of the compression spring is fixedly connected with the inner wall of the column cap, a sealing ring is fixedly arranged on the bottom surface of the push plate, a sieve plate is fixedly arranged in the sealing ring, a liquid flow conduit is arranged on the column cap in a threaded manner, the bottom surface of the liquid flow conduit penetrates through the push plate and is abutted against the sieve plate, the sealing ring is trapezoid, the sealing ring is tightly abutted against the top surface and the inner wall of the column tube, and a packing layer is filled in the column tube. After the screen plate is disassembled and assembled for many times, the compression spring and the push plate can still push the screen plate to ensure the sealing performance of the device, so that the liquid chromatographic column cannot be reduced in column efficiency due to the reduction of the sealing performance.

Description

Liquid chromatographic column

Technical Field

The utility model belongs to the technical field of liquid chromatography, and particularly relates to a liquid chromatography column.

Background

The liquid chromatography technology is a rapid preparation column chromatography form, which is based on the fact that pressure is generated by a pump to accelerate the elution speed of a mobile phase passing through a prefilled chromatographic separation column, the core component of the liquid chromatography column is the liquid chromatography column, and the separation principle is as follows: the sample of the mixture is dissolved in a mobile phase and is made to pass through the fixed phase adsorbent packed inside the liquid chromatographic column at certain speed, and the mixture components flow through the fixed phase adsorbent at different speed to reach the aim of separation.

The chromatographic column is a column tube packed with a stationary phase for separating the mixed components. Chromatography columns can be divided into packed columns and open-tube columns. Liquid chromatography typically employs packed columns.

The separation effect of a chromatographic column depends on the solid phase chosen, as well as the preparation and operating conditions of the chromatographic column. Chromatography is a separation analysis means, separation is the core, and thus the chromatographic column responsible for the separation is the heart of the chromatographic system. The requirements for chromatographic columns are high column efficiency, good selectivity, high analysis speed and the like. Commercially available various particulate fillers for HPLC such as porous silica gel and silica gel-based bonding phase, alumina, organic polymer microspheres (including ion exchange resins), porous carbon, etc., generally have particle sizes of 3,5,7,10 μm, and the theoretical column efficiency can reach 5-16 ten thousand/meter. For general analysis, only 5000-plate column efficiency is needed; for homolog analysis, only 500 is needed; for the substances which are difficult to separate, columns with the number of the column plates being up to 2 ten thousand can be adopted, so that the column length of about 10 cm to 30cm can meet the requirement of complex mixture analysis.

The chromatographic column consists of a column tube, a column cap, a clamping sleeve (sealing ring or sealing ring), a sieve plate (filter disc) and the like. The column joints at the two ends of the chromatographic column are internally provided with a sieve plate which is sintered stainless steel or titanium alloy and has a pore diameter of 0.2-20 mu m, and the purpose is to prevent the filler from leaking out according to the granularity of the filler. Clogging of chromatography column sieve plates is a common problem encountered during use of liquid chromatography. For serious blockage, the sieve plate needs to be taken down for cleaning or taken out for replacement, the sealing performance of the chromatographic column is obviously reduced after the sieve plate is disassembled and assembled for many times, and the column efficiency is reduced.

Disclosure of Invention

The utility model provides a liquid chromatographic column, which aims at solving the problems that the sealing performance of the existing liquid chromatographic column is obviously reduced and the column efficiency is reduced after the existing liquid chromatographic column is disassembled and assembled for a plurality of times.

In order to solve the problems, the utility model adopts the following technical scheme:

the utility model provides a liquid chromatographic column, includes the pillar, the upper and lower both ends of pillar all fixed mounting has the pillar cap, slidable mounting has the push pedal in the pillar cap, the top surface fixed mounting of push pedal has compression spring, compression spring's the other end with the inner wall fixed connection of pillar cap, the bottom surface fixed mounting of push pedal has the sealing washer, fixed mounting has the sieve in the sealing washer, threaded mounting has the liquid flow pipe on the pillar cap, the bottom surface of liquid flow pipe pierces through the push pedal and with the sieve offsets, the sealing washer is trapezoidal, the sealing washer with the orificial top surface of pillar and inner wall are tightly offset, the packing layer has been packed in the pillar.

For better sealing, the flow conduit is preferably screw-mounted with a conduit cap.

In order to prevent a large void from occurring between the packing and the inner wall of the column tube, it is preferable that the inner wall of the column tube is a concave-convex arc surface.

In order to increase the service life of the device, it is preferred that the vial and the cap are both made of polyetheretherketone material and the sealing ring is made of polyethylene material.

In order to facilitate clamping and fixing the column tube, preferably, the outer wall of the column tube is provided with a clamping notch surface.

Compared with the prior art, the utility model provides a liquid chromatographic column, which has the following beneficial effects:

the device is characterized in that the lower end of the sealing ring extends into the pipe orifice of the column pipe, the sealing ring deforms and tightly abuts against the inner wall of the column pipe, the compression spring compresses and generates downward elastic force to push the push plate to apply downward pressure, the compression deformation of the upper half part of the sealing ring tightly abuts against the top surface of the pipe orifice of the column pipe, the connection between the column pipe and the column cap is tightly sealed, the sealing effect of the device is improved, and after the screen plate is disassembled and assembled for many times, the compression spring and the push plate can still push the screen plate to ensure the sealing performance of the device, so that the liquid chromatographic column cannot be reduced due to the reduction of the sealing performance.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a liquid chromatography column according to the present utility model;

FIG. 2 is a schematic diagram of the structure of the liquid chromatographic column A in FIG. 1 according to the present utility model;

FIG. 3 is a schematic diagram of a seal ring structure of a liquid chromatography column according to the present utility model;

fig. 4 is a schematic cross-sectional view of a column tube of a liquid chromatography column according to the present utility model.

In the figure: 1. a column tube; 2. a cap; 3. a seal ring; 4. a push plate; 5. a compression spring; 6. a sieve plate; 7. a liquid flow conduit; 8. a catheter cap; 9. and a filler layer.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

In the description of the present utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

Examples:

referring to fig. 1-3, a liquid chromatographic column comprises a column tube 1, column caps 2 are fixedly installed at the upper end and the lower end of the column tube 1, a push plate 4 is slidably installed in the column caps 2, a compression spring 5 is fixedly installed on the top surface of the push plate 4, the other end of the compression spring 5 is fixedly connected with the inner wall of the column cap 2, a sealing ring 3 is fixedly installed on the bottom surface of the push plate 4, a sieve plate 6 is fixedly installed in the sealing ring 3, a liquid flow conduit 7 is installed on the column cap 2 in a threaded manner, the bottom surface of the liquid flow conduit 7 penetrates through the push plate 4 and abuts against the sieve plate 6, the sealing ring 3 is trapezoid, the sealing ring 3 abuts against the top surface and the inner wall of a mouth of the column tube 1 tightly, and a packing layer 9 is filled in the column tube 1.

The pipe cap 8 is arranged on the liquid flow pipe 7 in a threaded manner, and when the liquid flow pipe is not in use, the liquid flow pipe is sealed and protected through the pipe cap 8, so that impurities are prevented from entering the liquid flow pipe 7, and the detection is influenced.

The inner wall of the column tube 1 is a concave-convex arc surface, the packing layer 9 is filled with packing (i.e. stationary phase), when the packing is filled in the column tube 1, some areas are not tightly attached to the inner wall of the column tube 1, and gaps still exist, if the gaps are vertical downward, the detected target solution is likely to directly flow downward along the gaps, so that the detection result is inaccurate, the gaps between the packing layer 9 and the column tube 1 can be reduced due to the concave-convex arc surface, and the detected target solution cannot slide down directly.

The column tube 1 and the column cap 2 are both made of polyether-ether-ketone materials, the sealing ring 3 is made of polyethylene materials, and the polyether-ether-ketone has mechanical strength required by chromatographic environment, does not interfere with separation and purification processes, and has excellent chemical resistance; the polyethylene has good chemical stability and can resist most of acid and alkali.

The outer wall of the column tube 1 is provided with a clamping notch surface, and better fixing effect can be started through clamping the clamping notch surface on the column tube 1, so that the column tube 1 is not easy to topple over to be damaged.

The utility model is applied to the technical field of liquid chromatography, a to-be-detected object target solution and a mobile phase are injected into a liquid flow conduit 7 by opening a conduit cap 8 in a spiral manner, the liquid flow conduit 7 passes through a push plate 4 to be abutted against a sieve plate 6, the to-be-detected object target solution and the mobile phase are filtered through the sieve plate 6 and enter a packing layer 9, under a certain pressure, the to-be-detected object passes through the packing in the packing layer at a certain speed, at the moment, all components in the to-be-detected object target solution can flow through the packing layer 9 at different speeds because of different solubilities of a stationary phase and the mobile phase, separation of the to-be-detected object is realized, and then the to-be-detected object separated in a column tube 1 is detected by a detector of a liquid chromatograph, so that a detection result is obtained.

The present utility model is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present utility model and the inventive concept thereof, can be replaced or changed within the scope of the present utility model.

Claims

1. The utility model provides a liquid chromatography post, its characterized in that, includes pillar (1), the upper and lower both ends of pillar (1) are all fixed mounting has pillar cap (2), interior slidable mounting of pillar cap (2) has push pedal (4), the top surface fixed mounting of push pedal (4) has compression spring (5), the other end of compression spring (5) with the inner wall fixed connection of pillar cap (2), the bottom surface fixed mounting of push pedal (4) has sealing washer (3), fixed mounting has sieve (6) in sealing washer (3), threaded mounting has liquid flow conduit (7) on pillar cap (2), the bottom surface of liquid flow conduit (7) is penetrated push pedal (4) and with sieve (6) offset, sealing washer (3) are trapezoidal, sealing washer (3) with the orificial top surface of pillar (1) and inner wall tightly offset, packing layer (9) have been packed in pillar (1).

2. A liquid chromatography column according to claim 1, wherein the flow conduit (7) is screw-mounted with a conduit cap (8).

3. A liquid chromatography column according to claim 1, wherein the inner wall of the column tube (1) is a concave-convex arc surface.

4. A liquid chromatography column according to claim 1, wherein the column tube (1) and the column cap (2) are both made of polyetheretherketone material, and the sealing ring (3) is made of polyethylene material.

5. A liquid chromatography column according to claim 1, wherein the outer wall of the column tube (1) is provided with clamping notch surfaces.