CN213159493U - Preparation of liquid-phase pre-column - Google Patents
Preparation of liquid-phase pre-column Download PDFInfo
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- CN213159493U CN213159493U CN202021334186.1U CN202021334186U CN213159493U CN 213159493 U CN213159493 U CN 213159493U CN 202021334186 U CN202021334186 U CN 202021334186U CN 213159493 U CN213159493 U CN 213159493U
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Abstract
The utility model discloses a liquid-phase pre-column preparation, which is used for preliminarily purifying materials and prolonging the service life of a chromatographic preparation column, and relates to the technical field of auxiliary tools for liquid-phase chromatographic purification preparation; the utility model discloses a cylinder, support and top cap, the bottom of cylinder is connected with the support, and the top can be dismantled with the top cap and be connected, cylinder intracavity bottom level is provided with lower sieve, and it has the absorption purification filler to fill on this lower sieve, and the bottom of this cylinder is opened there is the liquid outlet, and top border equidistance is provided with the bolt hole, also open the bolt hole that corresponds with the cylinder on the top cap, and the inside level of this top cap is provided with upper sieve, and the top is provided with the inlet, top cap and cylinder pass through bolt seal connection; the utility model discloses reduce the harm of impurity to packing in the preparation liquid chromatography column, increase the life who prepares packing in the liquid chromatography column.
Description
Technical Field
The utility model provides a preparation liquid phase pre column for preliminary purification material, the life of extension chromatogram preparation post, the utility model relates to a preparation liquid chromatogram purification appurtenance technical field.
Background
Preparative liquid chromatography is a separation technique aimed at obtaining pure samples, and has been developed for a long time since the 20 th century and the 30 th century when applied to the separation of pigments to date; in the 20 th century, the high performance preparative liquid chromatography in 80 s had rapidly developed in the pharmaceutical industry, and in 90 s, the high performance preparative liquid chromatography was widely applied to the separation and purification of proteins; nowadays, preparative chromatography is applied in almost all fields of social production and life, such as separation and purification of secondary metabolites, natural products, macromolecular compounds, some important medicinal bioactive proteins, biological therapeutic products and diagnostic reagents; the preparation of liquid chromatography is commonly used for separation and purification of natural medicines, biochemical medicines, medicine impurities, proteins and other substances, but because the components of materials are complex, the filler is polluted by the impurities along with the purification of the materials, the problems of the increase of column pressure of a chromatographic column, the reduction of separation degree, the reduction of adsorption capacity and the like can be caused, and the service life of the filler is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a: the method for preparing the liquid phase pre-column is provided for preliminarily purifying the materials to be purified, reducing the damage of impurities to the filler in the prepared liquid phase chromatographic column and prolonging the service life of the filler in the prepared liquid phase chromatographic column.
The utility model adopts the technical scheme as follows: the utility model provides a preparation liquid phase pre-column, includes cylinder, support and top cap, the bottom and the leg joint of cylinder, the top can be dismantled with the top cap and be connected, cylinder intracavity bottom level is provided with down the sieve, and it has the absorption purification filler to fill on this lower sieve, and open the bottom of this cylinder has the liquid outlet, and top border equidistance is provided with the bolt hole, also open the bolt hole that corresponds with the cylinder on the top cap, the inside level of this top cap is provided with the sieve, and the top is provided with the inlet, the top cap passes through bolted seal with the cylinder and is connected.
The utility model discloses a theory of operation does: the utility model discloses carry out preliminary purification with the material, reduce the pollution of magazine to preparation liquid chromatography column chromatography filler in the material, increase the life who prepares liquid chromatography column filler, the utility model discloses cylinder and top cap bolted connection, be provided with the inlet on the top cap, the cylinder bottom is provided with the liquid outlet, the inlet is the same with liquid outlet shape, diameter, can replace each other and connect external equipment, one kind is when using, pack into the cylinder earlier with preparation liquid chromatography column filler similar filler, then with bolt and sealing washer cylinder and top cap sealing connection, the inlet on this top cap communicates with the equipment that is equipped with the material through the pipe, the pump is in the cylinder with the pump material, after the material is through upper screen plate, filler, lower screen plate preliminary purification roguing, flow into preparation liquid chromatography column from the liquid outlet of cylinder lower extreme and separate the purification, the material after the purification comes out from preparation liquid chromatography column lower extreme, entering a detector, and collecting the purified material; the other method is that the original conduit which is directly connected and pumped into the prepared liquid chromatographic column is connected with the liquid outlet at the lower end of the column body, and then the liquid inlet at the upper end of the column body is connected with the prepared liquid chromatographic column for use; the material to be purified flows through the preparative liquid chromatographic column first, and a part of impurities are adsorbed by the filler in the preparative column, so that the damage of the impurities to the filler in the preparative liquid chromatographic column is reduced, and the service life of the filler in the preparative liquid chromatographic column is prolonged.
A sealing ring is arranged between the top cover and the column body; the sealing effect is better.
The number of the bolt holes is 3-8, and the bolt holes are connected through bolts and are convenient to disassemble.
The support is provided with at least 3 supporting feet.
The top cover and the column body are both made of stainless steel materials; the service life is long.
The lower sieve plate and the upper sieve plate adopt 4000-5000 mesh sieve meshes, and the sieve meshes can be replaced according to the particle size of the filler, so that only the purified material flows out of the liquid outlet, and impurities and the filler are remained in the sieve meshes.
The adsorption purification filler is octadecylsilane chemically bonded silica.
To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:
the utility model discloses the material that needs the purification can flow through earlier and prepare liquid phase fore-line, and the impurity that can't be eluted is adsorbed by the filler in the fore-line, reduces the harm of impurity to filler in preparing the liquid chromatography post, increases the life who prepares filler in the liquid chromatography post.
Drawings
The invention will now be described, by way of example, with reference to the accompanying drawings, in which:
fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic top view of the structure of FIG. 1;
fig. 3 is a schematic top view of the top cover of the present invention when the top cover is opened;
labeled as: 1-column body, 2-top cover, 3-upper sieve plate, 4-lower sieve plate, 5-liquid inlet, 6-liquid outlet, 7-bolt, 8-bracket, 9-bolt hole and 10-sealing ring.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
As shown in fig. 1-3, a preparation liquid phase pre-column, includes cylinder 1, support 8 and top cap 2, the bottom and the support 8 of cylinder 1 are connected, and the top can be dismantled with top cap 2 and be connected, 1 intracavity bottom level of cylinder is provided with down sieve 4, and it has octadecylsilane chemically bonded silica gel to fill on this sieve 4 down, and the bottom of this cylinder 1 is opened there is liquid outlet 6, and top border equidistance is provided with bolt hole 9, also opened bolt hole 9 that corresponds with cylinder 1 on top cap 2, the inside level of this top cap 2 is provided with sieve 3, and the top is provided with inlet 5, top cap 2 and cylinder 1 pass through bolt 7 sealing connection.
The utility model discloses a theory of operation does: the utility model primarily purifies the material, reduces the pollution of magazines in the material to the preparation of the liquid chromatographic column packing, prolongs the service life of the preparation of the liquid chromatographic column packing, and the utility model discloses a cylinder 1 is bolted with a top cover 2, a liquid inlet 5 is arranged on the top cover 2, a liquid outlet 6 is arranged at the bottom of the cylinder 1, the shape and the diameter of the liquid inlet 5 and the liquid outlet 6 are the same, and the liquid inlet 5 and the liquid outlet 6 can be mutually replaced and connected with external equipment, one is that when in use, the cylinder 1 is firstly filled with the packing similar to the preparation of the liquid chromatographic column packing, then the cylinder 1 and the top cover 2 are hermetically connected by a bolt 7 and a sealing ring 10, the liquid inlet 5 on the top cover 2 is communicated with the equipment filled with the material by a conduit, the pump pumps the material into the cylinder 1, after the material is primarily purified by an upper sieve plate 3, the packing and a lower sieve plate 4, the purified material comes out from the lower end of the preparative liquid chromatography column and enters a detector, and the purified material is collected; the other method is that a conduit which is directly connected and pumped into the preparative liquid chromatography column is connected with a liquid outlet 6 at the lower end of a column body 1, and then a liquid inlet 5 at the upper end of the column body 1 is connected with the preparative liquid chromatography column for use; the material to be purified flows through the preparative liquid chromatographic column first, and a part of impurities are adsorbed by the filler in the preparative column, so that the damage of the impurities to the filler in the preparative liquid chromatographic column is reduced, and the service life of the filler in the preparative liquid chromatographic column is prolonged.
Example 2
On the basis of the embodiment 1, a sealing ring 10 is arranged between the top cover 2 and the column body 1; the sealing effect is better.
Example 3
On the basis of embodiment 1, the bolt holes 9 are 3-8 and are connected through bolts 7, so that the disassembly is convenient.
Example 4
On the basis of embodiment 1, the support 8 has at least 3 supporting feet.
Example 5
On the basis of the embodiment 1, the top cover 2 and the column body 1 are both made of stainless steel materials; the service life is long.
Example 6
On the basis of the embodiment 1, the lower sieve plate 4 and the upper sieve plate 3 adopt 4000-5000-mesh sieve meshes, and the sieve meshes can be replaced according to the particle size of the filler, so that only the purified material flows out of the liquid outlet 6, and impurities and the filler are all left in the sieve meshes.
The above description is only for the preferred embodiment of the present invention, and the present invention is not limited thereto, the protection scope of the present invention is defined by the claims, and all structural changes equivalent to the contents of the description and drawings of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The utility model provides a preparation liquid phase is post in advance, includes cylinder (1), support (8) and top cap (2), the bottom and the support (8) of cylinder (1) are connected, and the top can be dismantled with top cap (2) and be connected, its characterized in that, cylinder (1) intracavity bottom level is provided with down sieve (4), and it has the absorption purification filler to fill on this lower sieve (4), and liquid outlet (6) have been opened to the bottom of this cylinder (1), and top border equidistance is provided with bolt hole (9), also open bolt hole (9) that correspond with cylinder (1) on top cap (2), the inside level of this top cap (2) is provided with sieve (3), and the top is provided with inlet (5), top cap (2) pass through bolt (7) sealing connection with cylinder (1).
2. A pre-column for the preparation of a liquid phase according to claim 1, wherein a sealing ring (10) is provided between the top cover (2) and the column body (1).
3. A preparation pre-column for liquid phase according to claim 1, characterized in that the bolt holes (9) are provided in 3-8 numbers.
4. A preparative liquid phase pre-column according to claim 1, wherein the support (8) is at least 3 support feet.
5. A preparation pre-column for liquid phase according to claim 1, characterized in that the top cover (2) and the column body (1) are made of stainless steel material.
6. A method for preparing a liquid pre-column according to claim 1, wherein the lower sieve plate (4) and the upper sieve plate (3) are 4000-5000 mesh sieve.
7. The method of claim 1, wherein the adsorptive purification packing is octadecylsilane bonded silica.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021334186.1U CN213159493U (en) | 2020-07-09 | 2020-07-09 | Preparation of liquid-phase pre-column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021334186.1U CN213159493U (en) | 2020-07-09 | 2020-07-09 | Preparation of liquid-phase pre-column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213159493U true CN213159493U (en) | 2021-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021334186.1U Active CN213159493U (en) | 2020-07-09 | 2020-07-09 | Preparation of liquid-phase pre-column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213159493U (en) |
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2020
- 2020-07-09 CN CN202021334186.1U patent/CN213159493U/en active Active
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