WO2007025467A1 - Colonne de separation chromatographique a phase liquide - Google Patents

Colonne de separation chromatographique a phase liquide Download PDF

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Publication number
WO2007025467A1
WO2007025467A1 PCT/CN2006/002209 CN2006002209W WO2007025467A1 WO 2007025467 A1 WO2007025467 A1 WO 2007025467A1 CN 2006002209 W CN2006002209 W CN 2006002209W WO 2007025467 A1 WO2007025467 A1 WO 2007025467A1
Authority
WO
WIPO (PCT)
Prior art keywords
cylinder
separation column
liquid
wall
liquid chromatography
Prior art date
Application number
PCT/CN2006/002209
Other languages
English (en)
Chinese (zh)
Inventor
Ke Wang
Original Assignee
Ke Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ke Wang filed Critical Ke Wang
Publication of WO2007025467A1 publication Critical patent/WO2007025467A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6026—Fluid seals

Definitions

  • the present invention relates to a liquid chromatography separation column. Background technique
  • Liquid chromatography separation techniques are used to efficiently separate mixtures of mixtures with multiple components, the core of which is a liquid chromatography column.
  • the principle of separation is that a sample of the mixture is dissolved in a mobile phase and flows through the stationary phase adsorbent filled in the chromatographic separation column at a certain rate, because the adsorption force of the stationary phase adsorbent on different components is different. Therefore, the mixed components will flow through the stationary phase adsorbent at different rates to achieve the purpose of separation.
  • this chromatographic column once, if it needs to be reused, it is necessary to replace the new stationary phase adsorbent, which is not only troublesome, inefficient, but also affects the health of the operator.
  • the invention provides a column head body of the chromatographic column head which can be reliably sealed and connected with the cylinder body under pressure under the pressure of the cylinder body, and does not cause mobile phase leakage under a large working pressure, and does not occur.
  • a liquid chromatography separation column comprising a separation column body and a chromatography column head
  • the separation column body is composed of a cylinder, a stationary phase adsorbent filled in the cylinder body, and a capping phase in the stationary phase a filter for preventing leakage of the stationary phase adsorbent at both ends of the adsorbent and capable of passing the mobile phase
  • the tubular body has a liquid outlet at one end and an open end at the other end of the cylinder.
  • the column head comprises a column head body with a liquid inlet, an outer ring wall axially disposed on the end cover and a mast, the column head body being press-fitted on the open end side of the barrel body and sealingly connected with the inner wall of the barrel body, the end cap The upper outer ring wall is connected to the open end of the cylinder, and the mast is crimped onto the notch of the stud body.
  • the chromatographic column head of the invention consists of a split stigma body and an end cap, and is sealed and connected with the barrel body through the column head body. When the liquid chromatographic separation column is used under pressure, the stigma body receives a back pressure due to the back pressure in the cylinder body.
  • the invention has simple structure, is convenient for industrial production, and reduces manufacturing cost; the liquid chromatography separation column adopting the structure can be used once or repeatedly, and the structure effectively solves the problem of the cylinder and the chromatographic column head.
  • the chromatographic separation column of the present invention can withstand greater working pressure without causing leakage of the mobile phase.
  • the chromatographic separation column of the invention can be applied to a variety of manual or automatic fast liquid chromatography separation systems as a general-purpose color separation column, and has the advantages of simple operation, low use cost, and greatly improved production efficiency.
  • Figure 1 is a schematic view of the structure of the present invention.
  • Fig. 2 is a schematic enlarged view of the structure of Fig. 1;
  • the liquid chromatography separation column shown in Fig. 1 includes a separation column body 3, a filter 2, 4, and a column head 1.
  • the separation column body 3 is composed of a cylindrical body 31 and a stationary phase adsorption 32 filled in the cylindrical body 31, and a filter for preventing the stationary phase adsorbent 32 from leaking out at both ends of the stationary phase adsorbent 32 and allowing the mobile phase to pass therethrough. 2 and filter 4 are composed.
  • the cylinder 31 of the present invention is made of a high density plastic or other organic solvent resistant material, and the filters 2, 4 are generally made of a high density hydrophobic microporous plastic.
  • the cylindrical body 31 of the separation column body 3 of the present invention has a liquid outlet 33 at one end thereof and an open end at the other end of the cylinder 31.
  • the chromatographic column head 1 of the present invention comprises a stud body 12 with a liquid inlet port 11, an end cap 13 and an outer ring wall 14 and a mast 15 disposed axially on the end cap 13 as shown in Figs.
  • the mast 15 is arranged according to the geometry of the end cap 13.
  • the mast 15 of the present invention is coaxial with the outer ring wall 14 and may be disposed for different axes.
  • the stud body 12 and the end cap 13 may also be made of high-density plastic or other organic solvent-resistant material.
  • the stud body 12 is press-fitted on the open end side of the barrel 31 and is sealingly connected to the inner wall of the barrel 31, preferably the diameter of the stud body 12. It is larger than the inner wall diameter of the cylinder 31 by 0.1 mm to L5 mm.
  • the diameter of the column head body 12 may be equal to the diameter of the inner wall of the cylinder 31, and the matching visual material between the column head body 12 and the cylinder 31 and the pressure bearing capacity may be determined.
  • the stud main body 12 When the stud body 12 is pressed into the cylindrical body 31, the stud main body 12 cooperates with the inner wall of the cylindrical body 31 to achieve a sealed connection, and the end surface of the stud main body 12 on the side of the filter 2 may be provided with a radially outwardly diffusing groove 17
  • the groove 17 is evenly disposed, and the end of the groove 17 communicates with the liquid inlet port 11, which acts to allow the mobile phase liquid of the liquid inlet to flow more uniformly through the stationary phase adsorbent.
  • the outer ring wall 14 on the end cap 13 is attached to the open end side of the cylindrical body 31, and the mast 15 is crimped to the notch 16 of the stud body 12.
  • the end cap 13 of the chromatographic column head 1 of the present invention has an internal thread on the outer ring wall 14 and is connected to the outer side wall of the open end of the cylinder 31 to be connected thereto.
  • the present invention is in the end cap 13
  • the outer ring wall 14 can also be provided with a card slot or a buckle, and is connected with a corresponding buckle or a card slot on the outer side wall of the cylinder 31 to firmly connect the end cap 13 of the column head 1 with the barrel 31.
  • the notch 16 on the stud body 12 of the present invention is a communicating ring groove
  • the mast 15 of the end cover 13 is an annular wall as shown in FIG. 2, and has two inclined faces symmetrically disposed, and the two inclined faces are pressed against the stigma.
  • the sill 15 of the end cap 13 is an annular wall and has a unilateral outer slant which is pressed against the notch 16 of the stud body 12, the raft on the end cap 13 of the present invention.
  • the length of the column 15 can be adjusted, and the large-diameter portion of the slope is connected to the inner side surface of the end cover 13, or the large-diameter portion of the inclined surface thereof is connected to the annular wall as shown in FIG.
  • the notch 16 on the stud body 12 of the present invention may also be a discontinuous, symmetrically disposed notch 16, and the mast 15 on the end cap 13 is correspondingly disposed, and the groove on the stud body 12 is pressed by the inclined surface of the mast 15 On the mouth 16
  • the stud head 15 is inclined by the bevel 15 of the end cap 13 to make the stud body
  • the notch 16 of the 12 is radially deformed to form a tighter fit with the barrel 31, which improves the pressure bearing capacity of the column head 1 and the separation column body 3. As shown in FIG.
  • the present invention is provided with a reinforcing rib 34 on the inner end surface of the cylindrical body 31 on the side of the liquid outlet 33.
  • the reinforcing rib 34 is outwardly lowered from the liquid outlet, and the end surface of the rib 34 is reinforced.
  • the mechanical strength of the liquid outlet of the cylinder 31 can be improved, and on the other hand, since the end surface of the reinforcing rib 34 can be in contact with the filter 4, when the filter 4 is under pressure, the convexity is strengthened.
  • the support of the ribs 34 is less prone to deformation.
  • the filter 4 when assembling the liquid chromatography separation column, the filter 4 is first pressed into the cylinder 31, then loaded into the stationary phase adsorbent 32, pressed into another filter 2, and finally pressed into the chromatogram.
  • the column head 1 first presses the column head body 12 into the cylinder body 31 to be sealed and connected, and then the end cap 13 is screwed or snapped to the open end of the barrel body 31, so that the column head 1 is firmly connected to the barrel 31, and works.
  • the column head 1 and the separation column body 3 can achieve a reliable sealing performance and can withstand a large working pressure.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

L'invention concerne une colonne de séparation chromatographique à phase liquide qui comporte une tête et un corps de colonne, ce dernier étant formé d'un cylindre et d'absorbants en phase solide remplissant le cylindre ainsi que des disques de filtre placé de part et d'autre des adsorbants, afin d'éviter la fuite des adsorbants et de permettre l'écoulement de la phase à travers eux. Une extrémité du cylindre présente une sortie liquide et l'autre extrémité une entrée. La tête de colonne comprend un corps de tête doté d'une entrée d'alimentation, d'un couvercle et un arbre placé sur la paroi enveloppant externe du couvercle. Le corps de tête est installé sur le côté de l'extrémité ouverte du cylindre et relié avec la paroi interne du cylindre de manière hermétique. La paroi enveloppante externe du couvercle est reliée avec l'extrémité ouverte du cylindre, alors que la cale est plaquée dans la rainure ménagée sur le corps de tête. Avantageusement, la structure est conviviale, facile à fabriquer, la résistance à la pression est élevée, et permet d'éviter les fuites, le tout à moindre coût.
PCT/CN2006/002209 2005-08-27 2006-08-28 Colonne de separation chromatographique a phase liquide WO2007025467A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CNU2005200750235U CN2815464Y (zh) 2005-08-27 2005-08-27 液相色谱分离柱
CN200520075023.5 2005-08-27

Publications (1)

Publication Number Publication Date
WO2007025467A1 true WO2007025467A1 (fr) 2007-03-08

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PCT/CN2006/002209 WO2007025467A1 (fr) 2005-08-27 2006-08-28 Colonne de separation chromatographique a phase liquide

Country Status (2)

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CN (1) CN2815464Y (fr)
WO (1) WO2007025467A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110917659A (zh) * 2019-12-11 2020-03-27 福建师范大学福清分校 一种分散固相萃取装置
CN115524432A (zh) * 2022-09-22 2022-12-27 内蒙古仓颉科技有限公司 一种色谱分析仪智能分离样品系统

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102029078A (zh) * 2010-11-15 2011-04-27 利穗科技(苏州)有限公司 一种快拆玻璃预装柱
CN102841166A (zh) * 2011-06-20 2012-12-26 苏州赛分科技有限公司 一种液相色谱柱
CN103191585B (zh) * 2013-04-01 2016-01-27 田景振 一种具有报警功能的柱层析装置
CN104399282B (zh) * 2014-08-13 2017-04-05 源创精科生物科技(长沙)有限公司 一种手动层析柱
CN111579698A (zh) * 2020-07-03 2020-08-25 兰州东立龙信息技术有限公司 一种液相色谱柱及液相色谱系统

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN2087340U (zh) * 1991-01-09 1991-10-23 中国科学院大连化学物理研究所 高效液相色谱柱通用接头
CN2094745U (zh) * 1991-06-22 1992-01-29 中国科学院大连化学物理研究所 一种带保护柱的高效液相色谱柱
CN1366180A (zh) * 2002-03-07 2002-08-28 中国科学院大连化学物理研究所 一种锥形高效液相色谱制备柱

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2087340U (zh) * 1991-01-09 1991-10-23 中国科学院大连化学物理研究所 高效液相色谱柱通用接头
CN2094745U (zh) * 1991-06-22 1992-01-29 中国科学院大连化学物理研究所 一种带保护柱的高效液相色谱柱
CN1366180A (zh) * 2002-03-07 2002-08-28 中国科学院大连化学物理研究所 一种锥形高效液相色谱制备柱

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YU C. ET AL.: "Preparation of a Jacket Type Liquid-phase Chromatographic Separating Column", ANALYTICAL APPARATUS, no. 2, 1991, pages 42 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110917659A (zh) * 2019-12-11 2020-03-27 福建师范大学福清分校 一种分散固相萃取装置
CN110917659B (zh) * 2019-12-11 2021-07-02 福建师范大学福清分校 一种分散固相萃取装置
CN115524432A (zh) * 2022-09-22 2022-12-27 内蒙古仓颉科技有限公司 一种色谱分析仪智能分离样品系统
CN115524432B (zh) * 2022-09-22 2023-10-20 内蒙古仓颉科技有限公司 一种色谱分析仪智能分离样品系统

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