WO2007020611A3 - Cellules souches/progenitrices neurales humaines adultes provenant de l'epithelium olfactif et de la lamina propria olfactive, procede d'isolement, proliferation et differenciation dans un milieu de culture sans serum et utilisation pour la transplantation - Google Patents

Cellules souches/progenitrices neurales humaines adultes provenant de l'epithelium olfactif et de la lamina propria olfactive, procede d'isolement, proliferation et differenciation dans un milieu de culture sans serum et utilisation pour la transplantation Download PDF

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WO2007020611A3
WO2007020611A3 PCT/IB2006/052871 IB2006052871W WO2007020611A3 WO 2007020611 A3 WO2007020611 A3 WO 2007020611A3 IB 2006052871 W IB2006052871 W IB 2006052871W WO 2007020611 A3 WO2007020611 A3 WO 2007020611A3
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adult human
olfactory
human neural
proliferation
culture medium
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PCT/IB2006/052871
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WO2007020611A2 (fr
WO2007020611B1 (fr
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Ana Veronica Carvalhal
Carlos Lima
Vera Basto
Celso Cunha
Pedro Escada
Helder Cruz
Pedro Cruz
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Ecbio Investigacao E Desenvolv
Ana Veronica Carvalhal
Carlos Lima
Vera Basto
Celso Cunha
Pedro Escada
Helder Cruz
Pedro Cruz
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Application filed by Ecbio Investigacao E Desenvolv, Ana Veronica Carvalhal, Carlos Lima, Vera Basto, Celso Cunha, Pedro Escada, Helder Cruz, Pedro Cruz filed Critical Ecbio Investigacao E Desenvolv
Publication of WO2007020611A2 publication Critical patent/WO2007020611A2/fr
Publication of WO2007020611A3 publication Critical patent/WO2007020611A3/fr
Publication of WO2007020611B1 publication Critical patent/WO2007020611B1/fr

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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
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    • C12N2502/27—Lung cells, respiratory tract cells

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Abstract

L'invention concerne un procédé pour obtenir une culture de cellules souches/progénitrices neurales humaines adultes obtenues à partir de l'épithélium et de la lamina propria olfactive d'un adulte humain. Des cellules souches/progénitrices neurales humaines adultes ont été testées au moyen de 26 biopsies réalisées chez des hommes et des femmes entre 21 et 45 ans. L'invention concerne également une formulation de milieu de culture sans sérum qui permet la prolifération de cellules souches/progénitrices neurales humaines adultes pendant sept mois, dans des conditions sans sérum strictes, avec un temps de doublement de 15 jours. L'invention concerne en outre un nouveau procédé de mise en culture pour la prolifération de cellules souches/progénitrices neurales humaines adultes qui évite la formation de neurosphères. L'invention concerne de plus un procédé de différenciation de cellules souches/progénitrices neurales humaines adultes en neurones immatures. L'invention montre que les cellules humaines de l'épithélium olfactif constituent une source fiable pour la réparation de lésions tissulaires de la moelle épinière.
PCT/IB2006/052871 2005-08-19 2006-08-18 Cellules souches/progenitrices neurales humaines adultes provenant de l'epithelium olfactif et de la lamina propria olfactive, procede d'isolement, proliferation et differenciation dans un milieu de culture sans serum et utilisation pour la transplantation WO2007020611A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT103333 2005-08-19
PT10333305 2005-08-19

Publications (3)

Publication Number Publication Date
WO2007020611A2 WO2007020611A2 (fr) 2007-02-22
WO2007020611A3 true WO2007020611A3 (fr) 2007-09-13
WO2007020611B1 WO2007020611B1 (fr) 2007-10-25

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PCT/IB2006/052871 WO2007020611A2 (fr) 2005-08-19 2006-08-18 Cellules souches/progenitrices neurales humaines adultes provenant de l'epithelium olfactif et de la lamina propria olfactive, procede d'isolement, proliferation et differenciation dans un milieu de culture sans serum et utilisation pour la transplantation

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WO (1) WO2007020611A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008132722A1 (fr) 2007-04-26 2008-11-06 Ramot At Tel-Aviv University Ltd. Cellules souches autologues pluripotentes de muqueuse buccale et procédé d'utilisation
CN102228016B (zh) * 2011-04-18 2012-07-18 上海安集协康生物技术有限公司 一种神经干细胞冻存复苏的方法
WO2012164137A1 (fr) 2011-05-30 2012-12-06 Fundación Investigación En Regeneración Del Sistema Nervioso Cellules mères et cellules de la souche neurale dérivées de la glie olfactive enveloppante, et leurs applications
US20140242038A1 (en) * 2011-10-11 2014-08-28 The Trustees Of Columbia University In The City Of New York Method for generating beta cells
CN103958665A (zh) * 2011-11-11 2014-07-30 基本药品有限责任公司 包含血清替代物和不稳定因子的试剂盒
JP6243675B2 (ja) * 2012-09-20 2017-12-06 諭一郎 大西 オルファクトリースフィア細胞の生成・単離方法及び製造方法並びに該オルファクトリースフィア細胞を用いた脱随疾患治療剤及び末梢神経軸索再生増強剤の製造方法
KR101994640B1 (ko) * 2018-03-16 2019-07-02 (주)메디노 신경줄기세포의 고효율 분리배양 방법
CN112574941A (zh) * 2020-12-29 2021-03-30 上海派森诺生物科技股份有限公司 一种组织单细胞悬液的制备方法
CN116144598B (zh) * 2023-04-06 2023-09-01 中科中銮生物科技(广东)有限公司 一种促进神经干细胞增殖和分化的培养基及其应用

Citations (2)

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WO2003064601A2 (fr) * 2002-01-28 2003-08-07 University Of Louisville Research Foundation, Inc. Cellules souches olfactives humaines
WO2003089631A1 (fr) * 2002-04-22 2003-10-30 Griffith University Procede de propagation de cellules embryonnaires et/ou de cellules progenitrices

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2003064601A2 (fr) * 2002-01-28 2003-08-07 University Of Louisville Research Foundation, Inc. Cellules souches olfactives humaines
WO2003089631A1 (fr) * 2002-04-22 2003-10-30 Griffith University Procede de propagation de cellules embryonnaires et/ou de cellules progenitrices

Non-Patent Citations (2)

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Title
LIMA CARLOS ET AL: "Olfactory mucosa autografts in human spinal cord injury: a pilot clinical study.", THE JOURNAL OF SPINAL CORD MEDICINE. 2006, vol. 29, no. 3, 2006, pages 191 - 203 ; dis, XP009076460, ISSN: 1079-0268 *
ZHANG X ET AL: "Adult human olfactory neural progenitors cultured in defined medium", EXPERIMENTAL NEUROLOGY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 186, no. 2, April 2004 (2004-04-01), pages 112 - 123, XP004620562, ISSN: 0014-4886 *

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WO2007020611B1 (fr) 2007-10-25

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