WO2006133750A1 - Thermocycleur - Google Patents

Thermocycleur Download PDF

Info

Publication number
WO2006133750A1
WO2006133750A1 PCT/EP2006/001996 EP2006001996W WO2006133750A1 WO 2006133750 A1 WO2006133750 A1 WO 2006133750A1 EP 2006001996 W EP2006001996 W EP 2006001996W WO 2006133750 A1 WO2006133750 A1 WO 2006133750A1
Authority
WO
WIPO (PCT)
Prior art keywords
wall
lid
base body
thermocycler
working position
Prior art date
Application number
PCT/EP2006/001996
Other languages
German (de)
English (en)
Inventor
Henner Tasch
Bernd Krüger
Original Assignee
Eppendorf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Ag filed Critical Eppendorf Ag
Priority to JP2008516142A priority Critical patent/JP2008543292A/ja
Priority to CN2006800211735A priority patent/CN101198408B/zh
Priority to CA002610732A priority patent/CA2610732A1/fr
Priority to EP06707420A priority patent/EP1890816A1/fr
Priority to US11/916,616 priority patent/US20080210700A1/en
Publication of WO2006133750A1 publication Critical patent/WO2006133750A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation

Definitions

  • the invention relates to a thermal cycler according to the preamble of claim 1.
  • thermocycler usually refers to devices in which a PCR reaction can be carried out.
  • PCR is an established abbreviation known to those skilled in the art and directly understandable for a method in which target nucleic acids selectively contained in a sample can be amplified with a polymerase.
  • the invention relates to thermal cyclers, with which in real-time mode, the increase of the amplified products can be measured over time.
  • Thermocyclers of this type have a base body in which a receptable area for reaction vessels that is suitable for carrying out PCR reactions is formed. Furthermore, a lid is provided, which can be moved from an open position to a working position on the receiving area. An optical measuring device is provided on the cover, which in the working position is opposite the receiving region and can detect optical signals in the reaction vessels.
  • an exact temperature control of the reaction vessels is extremely important in PCR reactions. This applies in particular to real-time methods, since these methods generally also quantify and even slight deviations from the desired temperature profile can lead to false results. Partial temperature fluctuations due to secondary air influences must therefore be excluded. The same applies to the side of the light path between the optical measuring device and receiving area incident light that could affect the measurement.
  • the object of the invention is therefore to provide a thermal cycler, with a largely unaffected by external factors performing a PCR reaction, especially in real-time mode in a simple and secure manner is possible.
  • a receiving area is circumferentially formed, extending between the lid and the base body gas-tight wall provide, which is sealed against the lid and base body.
  • the wall according to the invention can also be provided in conventional thermocyclers. Preferably, however, it is provided in thermal cyclers, which can be operated in real-time mode. In these cases, in addition to the gas-tightness, the wall is also light-tight and surrounds in working position of the cover both the receiving area in the base body and the optical measuring device in the lid, so that an outwardly shielded light path and reaction space is formed.
  • the wall as a separate component which is placed on the base body prior to the start of the PCR and at e.g. Lowering the lid in working position with its upper open end comes into sealing engagement with the lid.
  • the wall is provided as connected to the thermal cycler component.
  • the wall it would be conceivable to provide the wall as a rigidly protruding component on the base body or cover. In this case, however, there is a risk that the handling is impaired. Therefore, it is preferable that the wall projects up and down only via the lid or base body during the execution of the PCR reaction of the length required for sealing. It would be conceivable, for example, that the wall in cases where it is not necessary to move in total in the lid or the base body.
  • the wall has a variably adjustable length. This could be achieved, for example, in a particularly preferred embodiment, by forming the wall as a bellows, which is fastened with its one open end, for example circumferentially on the lid and with its free open end by means of a suitable adjusting device in the working position of the lid except for the base body can be lowered.
  • a suitable adjusting device in the working position of the lid except for the base body can be lowered.
  • one or more circumferential seals for example made of silicone or the like, are provided at the free open of the wall or the bellows preferably used. Such seals can Of course, in all other cases on, or if it is a separate component is provided on both, open ends of the wall.
  • the operation of the bellows may e.g. via a device provided separately on the lid, e.g. a thumbwheel or the like, done. If the lid already controls are provided with which, for. an adjustment of the lid or a lock in working position, the operation of the adjustable wall can also be done via this device. One could then, e.g. Synchronize the operations such that at the same time the inventively provided wall between the lid and base station is extended during the process of the lid and locking in the working position with the dedicated actuator.
  • the wall can also be arranged in or on the base body. If adjustable in length, the wall does not necessarily have to be designed as a bellows. Also conceivable are other length-adjustable constructions, e.g. Slats, telescope etc.
  • thermocycler 10 shown schematically and in section, with a base body 11, in which a receiving area 12 for reaction vessels is provided.
  • a receiving area 12 for reaction vessels for temperature control of the receiving area 12 heaters 13 and 14, in particular Peltier elements are provided. Schematically indicated are recesses 15, can be used in the reaction vessels to be tempered.
  • recording areas in thermocyclers are designed to accommodate microtiter plates, which is not shown in the figure.
  • the thermal cycler 10 also has a cover 18, which is shown in the working position and rests on projections 19 arranged on the base body 11 in this position. Arranged on the cover 18 and looking downwards, an optical measuring device 20 is provided which has individual measuring openings 21 associated with the receptacles 15. With the optical measuring device 20, in each case the running in a reaction vessel amplification can be measured optically.
  • the wall 22 is attached at its one end to the lid 18.
  • the adjusting device 25 has actuators 26 which can be moved by a mechanism 27, not shown in detail upon actuation of an actuating device 28 in the direction of arrow 29 in the lid 18 and out of this. With their lower end, the actuators 26 engage a disposed on the free edge of the wall 22 circumferential seal 30, which is formed Jerusalemspreizend down to particularly effective to prevent light entry.
  • the wall Upon actuation of the device 28, the wall can thus be lowered as needed from the lid 18 on the base body 11 for sealing or moved into a rest position in the vicinity of the lid 18.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Optical Measuring Cells (AREA)

Abstract

L'invention concerne un thermocycleur qui comprend un élément de base pourvu d'une zone de réception pouvant être tempérée pour des réacteurs et un couvercle pouvant être mis en une position de travail par l'intermédiaire de la zone de réception. En position de travail du couvercle, une paroi étanche aux gaz s'étendant entre le couvercle et l'élément de base et entourant la zone de réception est hermétique relativement au couvercle et à l'élément de base.
PCT/EP2006/001996 2005-06-13 2006-03-04 Thermocycleur WO2006133750A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2008516142A JP2008543292A (ja) 2005-06-13 2006-03-04 サーモサイクラ装置
CN2006800211735A CN101198408B (zh) 2005-06-13 2006-03-04 热循环器
CA002610732A CA2610732A1 (fr) 2005-06-13 2006-03-04 Thermocycleur
EP06707420A EP1890816A1 (fr) 2005-06-13 2006-03-04 Thermocycleur
US11/916,616 US20080210700A1 (en) 2005-06-13 2006-03-04 Thermocycler

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005027407.2 2005-06-13
DE102005027407A DE102005027407B3 (de) 2005-06-13 2005-06-13 Thermocycler

Publications (1)

Publication Number Publication Date
WO2006133750A1 true WO2006133750A1 (fr) 2006-12-21

Family

ID=36570726

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/001996 WO2006133750A1 (fr) 2005-06-13 2006-03-04 Thermocycleur

Country Status (7)

Country Link
US (1) US20080210700A1 (fr)
EP (1) EP1890816A1 (fr)
JP (1) JP2008543292A (fr)
CN (1) CN101198408B (fr)
CA (1) CA2610732A1 (fr)
DE (1) DE102005027407B3 (fr)
WO (1) WO2006133750A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008194042A (ja) * 2007-02-13 2008-08-28 Eppendorf Ag 試料の大きさに依存しない高さ調整装置を備えた試料カバー
JP2011512139A (ja) * 2008-02-15 2011-04-21 バイオ−ラッド ラボラトリーズ，インコーポレイティド 自動調整する蓋を有するサーマルサイクラー
US8492137B2 (en) 2007-02-13 2013-07-23 Eppendorf Ag Cover for sample with homogenous pressure application

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005027555B3 (de) * 2005-06-14 2006-10-05 Eppendorf Ag Thermocycler
DE102014108143A1 (de) * 2014-06-10 2015-12-10 Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh An optical system for detecting fluorescent or luminescent signals of at least two samples
DE102016200271A1 (de) 2016-01-13 2017-07-13 Institut Dr. Foerster Gmbh & Co. Kg Vorrichtung zur Erzeugung und Messung einer Emission

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602756A (en) * 1990-11-29 1997-02-11 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
DE10205977A1 (de) * 2002-02-08 2003-08-28 Eppendorf Ag Workstation zum Durchführen von chemischen und biologischen Verfahren in Mikrotiterplatten
WO2003089136A1 (fr) * 2002-04-19 2003-10-30 Pamgene B.V. Plaque a substrat, procede et appareil utilises pour fabriquer une plaque a substrat et systeme de mise en oeuvre de bioessais au moyen d'une telle plaque a substrat
US20030235517A1 (en) * 2000-09-19 2003-12-25 Holgar Deppe Incubation device
US20040149725A1 (en) * 1999-07-30 2004-08-05 Stratagene Apparatus and method for thermally cycling samples of biological material with substantial temperature uniformity

Family Cites Families (11)

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Publication number Priority date Publication date Assignee Title
US7560273B2 (en) * 2002-07-23 2009-07-14 Applied Biosystems, Llc Slip cover for heated platen assembly
JPH0523900U (ja) * 1991-09-13 1993-03-30 公和 池亀 食品衛生用インジケータ
CH688987A5 (de) * 1996-11-13 1998-07-15 Doebelin Werner Reaktionskammer fuer die chemische Synthese oder verwandte Anwendungen.
CA2328609A1 (fr) * 1998-05-16 1999-11-25 Pe Corporation (Ny) Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn
US6238627B1 (en) * 1998-08-26 2001-05-29 Arqule, Inc. Reaction block and cover
JP2001337039A (ja) * 2000-05-26 2001-12-07 Dkk Toa Corp 発光検出装置
DE10131687A1 (de) * 2001-06-29 2003-01-16 Eppendorf Ag Vorrichtung zur Durchführung von Nukleinsäure-Amplifikationsreaktionen bei gleichzeitiger Verfolgung der Bildung von Amplifikationsprodukten
EP2420824B1 (fr) * 2001-06-29 2018-11-28 Meso Scale Technologies LLC Plaque multipuits ayant un réseau de puits et un kit pour l'utilisation dans la conduite d'un essai ECL
JP3811393B2 (ja) * 2001-11-28 2006-08-16 松下電器産業株式会社 蛍光検出装置
US20060013736A1 (en) * 2002-04-19 2006-01-19 Blok Herman J System, substrate plate and incubation device for conducting bioassays
DE10232202B4 (de) * 2002-07-16 2005-08-25 H+P Labortechnik Ag Probenbehandlungsstation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602756A (en) * 1990-11-29 1997-02-11 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US20040149725A1 (en) * 1999-07-30 2004-08-05 Stratagene Apparatus and method for thermally cycling samples of biological material with substantial temperature uniformity
US20030235517A1 (en) * 2000-09-19 2003-12-25 Holgar Deppe Incubation device
DE10205977A1 (de) * 2002-02-08 2003-08-28 Eppendorf Ag Workstation zum Durchführen von chemischen und biologischen Verfahren in Mikrotiterplatten
WO2003089136A1 (fr) * 2002-04-19 2003-10-30 Pamgene B.V. Plaque a substrat, procede et appareil utilises pour fabriquer une plaque a substrat et systeme de mise en oeuvre de bioessais au moyen d'une telle plaque a substrat

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008194042A (ja) * 2007-02-13 2008-08-28 Eppendorf Ag 試料の大きさに依存しない高さ調整装置を備えた試料カバー
EP1964609A1 (fr) * 2007-02-13 2008-09-03 Eppendorf Ag Couvercle pour échantillons avec un réglage de hauteur indépendant de la taille des échantillons
EP2359933A1 (fr) * 2007-02-13 2011-08-24 Eppendorf AG Couvercle pour échantillons avec un réglage de hauteur indépendant de la taille des échantillons
AU2008200653B2 (en) * 2007-02-13 2012-12-06 Eppendorf Ag Cover for sample with sample-size independent height adjustment
US8492137B2 (en) 2007-02-13 2013-07-23 Eppendorf Ag Cover for sample with homogenous pressure application
US9289769B2 (en) 2007-02-13 2016-03-22 Eppendorf Ag Cover for sample with sample-size independent height adjustment
JP2011512139A (ja) * 2008-02-15 2011-04-21 バイオ−ラッド ラボラトリーズ，インコーポレイティド 自動調整する蓋を有するサーマルサイクラー
US8784753B2 (en) 2008-02-15 2014-07-22 Bio-Rad Laboratories, Inc. Thermal cycler with self-adjusting lid

Also Published As

Publication number Publication date
CN101198408A (zh) 2008-06-11
US20080210700A1 (en) 2008-09-04
EP1890816A1 (fr) 2008-02-27
JP2008543292A (ja) 2008-12-04
CA2610732A1 (fr) 2006-12-21
DE102005027407B3 (de) 2006-11-09
CN101198408B (zh) 2010-05-19

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