WO2006104035A8 - 生体分子固定化用の三脚型機能性界面分子とこれを用いた遺伝子検出デバイス - Google Patents

生体分子固定化用の三脚型機能性界面分子とこれを用いた遺伝子検出デバイス Download PDF

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WO2006104035A8
WO2006104035A8 PCT/JP2006/305959 JP2006305959W WO2006104035A8 WO 2006104035 A8 WO2006104035 A8 WO 2006104035A8 JP 2006305959 W JP2006305959 W JP 2006305959W WO 2006104035 A8 WO2006104035 A8 WO 2006104035A8
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WIPO (PCT)
Prior art keywords
molecule
bound
gene
nucleic acid
metal electrode
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PCT/JP2006/305959
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English (en)
French (fr)
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WO2006104035A1 (ja
WO2006104035A9 (ja
Inventor
純夫 丸山
利弥 坂田
大塚　英典
宮原　裕二
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独立行政法人物質・材料研究機構
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Publication of WO2006104035A1 publication Critical patent/WO2006104035A1/ja
Publication of WO2006104035A9 publication Critical patent/WO2006104035A9/ja
Publication of WO2006104035A8 publication Critical patent/WO2006104035A8/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/12—Amino derivatives of triarylmethanes without any OH group bound to an aryl nucleus

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　炭素原子の３個の結合手にチオール基を含む原子団を結合配置し、他の一つにオリゴヌクレオチオド結合性基を含む原子団を結合配置した三脚型機能性界面分子を基板上に配向させて核酸を前記のオリゴヌクレオチオド結合性基に結合固定化し、効率的にハイブリダイゼーションを行うことを可能とし、遺伝子検出デバイスでは、チャネル部近傍に形成された金属電極側面に一本鎖核酸プローブを固定化し、該金属電極表面でハイブリダイゼーションを行わせて二本鎖ＤＮＡを形成し、さらに横方向伸長反応など分子生物学的工程を行い、半導体表面のキャリア密度変化に伴う電気的信号を検出する。高感度・高精度に遺伝子測定が可能で、かつ低価格システムを実現できる核酸固定化用の技術手段とこれを用いた遺伝子検出デバイスとする。
PCT/JP2006/305959 2005-03-29 2006-03-24 生体分子固定化用の三脚型機能性界面分子とこれを用いた遺伝子検出デバイス WO2006104035A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005093399A JP4706074B2 (ja) 2005-03-29 2005-03-29 生体分子固定化用の三脚型機能性界面分子とこれを用いた遺伝子検出デバイス
JP2005-093399 2005-03-29

Publications (3)

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WO2006104035A1 WO2006104035A1 (ja) 2006-10-05
WO2006104035A9 WO2006104035A9 (ja) 2009-01-08
WO2006104035A8 true WO2006104035A8 (ja) 2009-08-27

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PCT/JP2006/305959 WO2006104035A1 (ja) 2005-03-29 2006-03-24 生体分子固定化用の三脚型機能性界面分子とこれを用いた遺伝子検出デバイス

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JP (1) JP4706074B2 (ja)
WO (1) WO2006104035A1 (ja)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4921213B2 (ja) * 2007-03-19 2012-04-25 キヤノン株式会社 検出素子、検出素子装置及び検出方法
JP5300205B2 (ja) * 2007-03-22 2013-09-25 キヤノン株式会社 標的物質検出素子、標的物質検出方法、標的物質検出素子の製造方法
JP5093661B2 (ja) * 2007-12-06 2012-12-12 独立行政法人産業技術総合研究所 生体関連物質センシングのための基板及びこれを用いたタンパク、金属イオン等の回収方法もしくは検出方法
KR20200123465A (ko) * 2018-02-20 2020-10-29 프로미넌트 메디칼 인코포레이션 산화 알루미늄 표면 및 계면 분자

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0493642A1 (en) * 1991-01-02 1992-07-08 Takeo Dr. Takayanagi Novel sulfonyl derivatives
US5945293A (en) * 1997-10-09 1999-08-31 Coulter International Corp. Protein-colloidal metal-aminodextran coated particle and methods of preparation and use
KR100383080B1 (ko) * 2000-09-05 2003-05-12 주식회사 포스코 조절된 아민기 밀도와 공간을 제공하는 분자층을 표면에포함하는 기질 및 이의 제조방법
JP3946701B2 (ja) * 2001-12-19 2007-07-18 株式会社日立ハイテクノロジーズ ポテンシオメトリックｄｎａマイクロアレイ、その製造方法、及び核酸解析方法

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JP4706074B2 (ja) 2011-06-22
WO2006104035A1 (ja) 2006-10-05
WO2006104035A9 (ja) 2009-01-08
JP2006275670A (ja) 2006-10-12

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