WO2009039549A3 - Verfahren zur bestimmung von mikroorganismen - Google Patents

Verfahren zur bestimmung von mikroorganismen Download PDF

Info

Publication number
WO2009039549A3
WO2009039549A3 PCT/AT2008/000348 AT2008000348W WO2009039549A3 WO 2009039549 A3 WO2009039549 A3 WO 2009039549A3 AT 2008000348 W AT2008000348 W AT 2008000348W WO 2009039549 A3 WO2009039549 A3 WO 2009039549A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
microorganism
probe
nucleic acid
nucleic acids
Prior art date
Application number
PCT/AT2008/000348
Other languages
English (en)
French (fr)
Other versions
WO2009039549A2 (de
Inventor
Herbert Wiesinger-Mayr
Original Assignee
Herbert Wiesinger-Mayr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Herbert Wiesinger-Mayr filed Critical Herbert Wiesinger-Mayr
Priority to EP08799937A priority Critical patent/EP2198056A2/de
Publication of WO2009039549A2 publication Critical patent/WO2009039549A2/de
Publication of WO2009039549A3 publication Critical patent/WO2009039549A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Identifizierung mindestens eines Mikroorganismus in einer Probe umfassend die Schritte: a) Bereitstellen einer Probe; b) Isolierung von in der Probe enthaltenen Nukleinsäuren; c) Inkontaktbringen der isolierten Nukleinsäuren mit mindestens einer Sonde, die an das 16S rRNA- und/oder 18S rRNA-Gen der Nukleinsäure des mindestens einen zu identifizierenden Mikroorganismus bindet, und ausgewählt ist aus der Gruppe bestehend aus den Nukleinsäuresequenzen SEQ ID Nr. 1 bis 831 oder deren revers-komplementären Sequenzen wobei die mindestens eine Sonde an einem festen Träger immobilisiert ist; d) Nachweisen einer Bindung der isolierten Nukleinsäure an die mindestens eine Sonde und e) Identifizieren eines Mikroorganismus in der Probe durch Korrelieren der nachgewiesenen Bindung der isolierten Nukleinsäuren mit den immobilisierten Sonden mit den in Tabelle A angeführten Mikroorganismen.
PCT/AT2008/000348 2007-09-27 2008-09-29 Verfahren zur bestimmung von mikroorganismen WO2009039549A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08799937A EP2198056A2 (de) 2007-09-27 2008-09-29 Verfahren zur bestimmung von mikroorganismen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0152907A AT505849B1 (de) 2007-09-27 2007-09-27 Verfahren zur bestimmung von mikroorganismen
ATA1529/2007 2007-09-27

Publications (2)

Publication Number Publication Date
WO2009039549A2 WO2009039549A2 (de) 2009-04-02
WO2009039549A3 true WO2009039549A3 (de) 2009-07-30

Family

ID=40225324

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT2008/000348 WO2009039549A2 (de) 2007-09-27 2008-09-29 Verfahren zur bestimmung von mikroorganismen

Country Status (3)

Country Link
EP (1) EP2198056A2 (de)
AT (1) AT505849B1 (de)
WO (1) WO2009039549A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102094089B (zh) * 2010-12-06 2013-09-11 北京大北农科技集团股份有限公司 用于鉴定短小乳杆菌的引物序列及其应用
DE102011118949B3 (de) * 2011-11-16 2012-10-25 Universität Rostock Kombiniertes Verfahren zum differentialdiagnostischen Nachweis von Bakterien des Mycobacterium-tuberculosis-Komplexes und von atypischen Pneumonie-Erregern
CN102605084B (zh) * 2012-03-29 2014-07-02 昆明理工大学 荧光原位杂交检测蜡样芽胞杆菌与枯草芽孢杆菌的方法
US9303281B2 (en) 2012-07-23 2016-04-05 Pall Corporation Compositions for detecting foodstuff spoilage microorganisms
US11713482B2 (en) 2016-06-15 2023-08-01 Genecapture, Inc. Compositions, methods and devices comprising stem-loop captor molecules
CN107130022A (zh) * 2017-05-05 2017-09-05 广西壮族自治区兽医研究所 一种检测隐秘杆菌的实时定量lamp引物、试剂盒及方法
EP3841223A1 (de) * 2018-08-08 2021-06-30 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis von mycoplasma genitalium
CN109337996A (zh) * 2018-12-24 2019-02-15 福建省农业科学院畜牧兽医研究所 一种用于检测山羊化脓隐秘杆菌的引物和探针
CN109666753A (zh) * 2019-03-01 2019-04-23 福建省农业科学院畜牧兽医研究所 一种检测3种羊致病性支原体的三重荧光定量pcr引物和探针
CN110172420B (zh) * 2019-05-15 2022-03-01 福建省农业科学院农业工程技术研究所 一株鼠李糖乳杆菌及其应用
CN110564874B (zh) * 2019-08-08 2022-10-14 河北省食品检验研究院（国家果类及农副加工产品质量监督检验中心、河北省食品安全实验室） 一种针对发酵乳中以葡萄糖杆菌为主的系列杂菌污染的高精度特异性数字pcr检测方法
KR102336532B1 (ko) * 2020-08-11 2021-12-06 건국대학교 산학협력단 대장균, 대장균군 및 총균의 표적 서열 증폭을 위한 프라이머 세트 및 이의 용도
CN111793586B (zh) * 2020-08-13 2022-05-20 石河子大学 一种母乳源戊糖片球菌及其应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948899A (en) * 1987-09-21 1999-09-07 Gen-Probe Incorporated Compositions for homogenous protection assay
WO2003106676A1 (ja) * 2002-06-14 2003-12-24 日立ソフトウエアエンジニアリング株式会社 微生物同定用プローブ及びそれを用いた同定方法
WO2004033720A2 (en) * 2002-10-09 2004-04-22 Hôpitaux Universitaires De Geneve Analytical chip for detection of 16s-rrna from clinically relevant bacteria and analytical method based thereon
CN1519328A (zh) * 2003-01-21 2004-08-11 中国人民解放军军事医学科学院微生物 一种用于临床细菌检测的生物芯片
US20050026206A1 (en) * 1999-05-03 2005-02-03 Hogan James J. Probe matrix-based device for identifying microorganisms
EP1803823A2 (de) * 2005-12-09 2007-07-04 Canon Kabushiki Kaisha Sondensatz, immobilisierter Sondenträger und Genuntersuchungsverfahren
WO2008003114A2 (en) * 2006-07-05 2008-01-10 Austrian Research Centers Gmbh - Arc Identification of pathogens

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948899A (en) * 1987-09-21 1999-09-07 Gen-Probe Incorporated Compositions for homogenous protection assay
US20050026206A1 (en) * 1999-05-03 2005-02-03 Hogan James J. Probe matrix-based device for identifying microorganisms
WO2003106676A1 (ja) * 2002-06-14 2003-12-24 日立ソフトウエアエンジニアリング株式会社 微生物同定用プローブ及びそれを用いた同定方法
WO2004033720A2 (en) * 2002-10-09 2004-04-22 Hôpitaux Universitaires De Geneve Analytical chip for detection of 16s-rrna from clinically relevant bacteria and analytical method based thereon
CN1519328A (zh) * 2003-01-21 2004-08-11 中国人民解放军军事医学科学院微生物 一种用于临床细菌检测的生物芯片
EP1803823A2 (de) * 2005-12-09 2007-07-04 Canon Kabushiki Kaisha Sondensatz, immobilisierter Sondenträger und Genuntersuchungsverfahren
WO2008003114A2 (en) * 2006-07-05 2008-01-10 Austrian Research Centers Gmbh - Arc Identification of pathogens

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 1 September 2006 (2006-09-01), "Aeromonas sp. SBRD8 16S ribosomal RNA gene, partial sequence.", XP002511625, retrieved from EBI accession no. EMBL:DQ539496 Database accession no. DQ539496 *
DATABASE Geneseq [online] 10 March 2005 (2005-03-10), "Bacterial 16S rRNA sequence #117.", XP002511621, retrieved from EBI accession no. GSN:ADW08361 Database accession no. ADW08361 *
DATABASE Geneseq [online] 10 March 2005 (2005-03-10), "Bacterial 16S rRNA sequence #23.", XP002511622, retrieved from EBI accession no. GSN:ADW08267 Database accession no. ADW08267 *
DATABASE Geneseq [online] 11 March 2004 (2004-03-11), "Microorganism detection probe, SEQ ID 54.", XP002511624, retrieved from EBI accession no. GSN:ADF93961 Database accession no. ADF93961 *
DATABASE Geneseq [online] 11 March 2004 (2004-03-11), "Microorganism detection probe, SEQ ID 75.", XP002511623, retrieved from EBI accession no. GSN:ADF93982 Database accession no. ADF93982 *
DATABASE Geneseq [online] 28 October 1999 (1999-10-28), "E. coli 16S rRNA subunit AE labelled probe 1.", XP002511626, retrieved from EBI accession no. GSN:AAZ09291 Database accession no. AAZ09291 *
DATABASE WPI Week 200407, Derwent World Patents Index; AN 2004-071565, XP002511628 *
DATABASE WPI Week 200479, Derwent World Patents Index; AN 2004-796986, XP002511627 *
WANG XIN-WEI ET AL: "Development and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY AUG 2007, vol. 76, no. 1, August 2007 (2007-08-01), pages 225 - 233, XP002511620, ISSN: 0175-7598 *

Also Published As

Publication number Publication date
WO2009039549A2 (de) 2009-04-02
EP2198056A2 (de) 2010-06-23
AT505849B1 (de) 2010-01-15
AT505849A1 (de) 2009-04-15

Similar Documents

Publication Publication Date Title
WO2009039549A3 (de) Verfahren zur bestimmung von mikroorganismen
WO2008028210A3 (de) Bakteriennachweis
WO2008003114A3 (en) Identification of pathogens
WO2007023461A3 (en) Method for detection of micro-organisms and antibiotic resistance markers and nucleic acid oligonucleotides therefor
NZ552462A (en) Method for controlling the microbiological quality of an aqueous medium and kit therefor
EP1716257B8 (de) Neue primer und sonden zum nachweis von parvovirus b19
DE60131284D1 (de) Methode zum nachweis von mikroorganismen
WO2009007719A3 (en) Detection of micro-organisms based on their nad-dependent dna ligase activity
EP1746156A4 (de) Sonde und primer zum nachweis von tuberkelbazillen sowie verfahren zum nachweis von tuberkelbazillen beim menschen damit
JP2010227103A5 (de)
WO2006088910A3 (en) Sequence-specific detection of nucleotide sequences
WO2002022890A3 (en) Compositions, methods and kits for determining the presence of cryptosporidium organisms in a test sample
WO2001001144A3 (en) Hybridization of target dna with immobilized nucleic acid analogs
WO2011082325A3 (en) Sequences of e.coli 055:h7 genome
EP2479273A3 (de) Primer und Sonde zur Erkennung von Mykobakterium Avium und Verfahren zur Erkennung von Mykobakterium Avium damit
WO2008102760A1 (ja) ａｂｌ遺伝子変異の検出用プローブおよびその用途
NZ589680A (en) Nucleic acids and methods for detecting turfgrass pathogenic fungi
WO2008061311A3 (en) Improved hybridisation of nucleic acids
MY149655A (en) Detection and enumeration of microorganisms
TW200734465A (en) Oligonucleotides, arrays thereof for detecting microorganisms, and apparatus, method and kit for detecting microorganisms
CA2385652A1 (en) Method and nucleic acids for the detection of microorganisms relevant to brewing
US20170081709A1 (en) Method for detecting escherichia coli and carrier for detecting escherichia coli
JP6530198B2 (ja) 乳酸菌の検出方法、乳酸菌検出キット、及び乳酸菌検出器具
WO2009043937A3 (en) Method for detecting ralstonia solanacearum race 3 biovar 2
WO2011090875A3 (en) Primers, sequences and recombinant probes for identification of mycobacterium species

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08799937

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008799937

Country of ref document: EP