WO2006007884A1 - Dispositif de refroidissement pour echantillons biologiques - Google Patents

Dispositif de refroidissement pour echantillons biologiques Download PDF

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Publication number
WO2006007884A1
WO2006007884A1 PCT/EP2005/003162 EP2005003162W WO2006007884A1 WO 2006007884 A1 WO2006007884 A1 WO 2006007884A1 EP 2005003162 W EP2005003162 W EP 2005003162W WO 2006007884 A1 WO2006007884 A1 WO 2006007884A1
Authority
WO
WIPO (PCT)
Prior art keywords
cooling device
coolant
cooling
space
wall
Prior art date
Application number
PCT/EP2005/003162
Other languages
German (de)
English (en)
Inventor
Young-Joo Oh
Günter FUHR
Heiko Zimmermann
Uwe Schön
Original Assignee
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. filed Critical Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Priority to US10/598,436 priority Critical patent/US20070169488A1/en
Priority to DK05716364T priority patent/DK1768782T3/da
Priority to DE502005004582T priority patent/DE502005004582D1/de
Priority to EP05716364A priority patent/EP1768782B1/fr
Priority to PL05716364T priority patent/PL1768782T3/pl
Publication of WO2006007884A1 publication Critical patent/WO2006007884A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/02—Water baths; Sand baths; Air baths
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D29/00—Arrangement or mounting of control or safety devices
    • F25D29/001—Arrangement or mounting of control or safety devices for cryogenic fluid systems
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
    • F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
    • F25D3/102—Stationary cabinets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling

Definitions

  • the invention relates to a cooling device for biological samples, in particular for the investigation, manipulation and processing of cryoprobes, according to the preamble of claim 1.
  • cryoprobes wherein the vitality-preserving storage of such cryoprobes takes place in so-called cryotanks in which there is liquid nitrogen.
  • a cooling device which can consist, for example, of a trough, at the bottom of which there is liquid nitrogen, which is also referred to as nitrogen and evaporates slowly, so that the cryoprobe in the tub continues to be sufficiently cooled.
  • a transparent protective bell can be placed on the tub, with glove cuffs being able to be manipulated in the wall of the protective bell, by means of which an operator can manipulate the cryoprobe in the tub ,
  • a further disadvantage of the known cooling device is the fact that the protective bell can mist due to nitrogen outgassing nitrogen from the nitrogen, which considerably complicates the visual inspection.
  • the temperature within the tub in the known cooling device can not or only with difficulty be adjusted by a change in the amount of liquid nitrogen introduced into the tub.
  • the object of the invention is therefore to improve the cooling device described in the introduction accordingly.
  • a further aim may be to improve the temperature consistency in the cooling device, to enable the adjustment of the temperature, to optimize the temperature distribution within the cooling device, to avoid fogging of the protective bell and to minimize the moisture in the cooling space ,
  • the invention is based on the technical knowledge that the unsatisfactory temperature stability and distribution within the cooling trough in the known cooling device is caused by the fact that the nitrogen outgassing nitrogen from the nitrogen diffuses in an undefined manner, so that the desired working temperature is reached difficult to achieve and difficult to regulate.
  • the uncontrolled outgassing of nitrogen from the nitrogen lake also leads to the fogging of the attached protective bell.
  • the invention therefore comprises the general technical teaching to avoid a nitrogen lake in the cooling device and instead to introduce gaseous nitrogen in a controlled manner into the cooling space.
  • the cooling device therefore has a cooling space for receiving cooling goods, which is delimited by an inner wall and an outer wall, wherein between the inner wall and the outer wall there is a gap into which a coolant supply line opens.
  • the coolant for example liquid nitrogen
  • the coolant is therefore not introduced directly into the cooling space, but into the space between the inner wall and the outer wall of the cooling space, the inner wall being permeable to the coolant, so that the coolant flows out the space between the outer wall and the inner wall through the inner wall back into the cold room occurs.
  • a buffer material is arranged in the space between the réellewan ⁇ and the outer wall of the cooling space, which temporarily receives the introduced into the gap coolant and emits continuously through the inner wall into theméraur ⁇ .
  • the buffer material is therefore preferably porous in order, for example, to be able to temporarily store liquid nitrogen.
  • the outer wall of the cooling space is preferably impermeable to the coolant in order to prevent the coolant from escaping to the outside into the environment.
  • the external migration preferably thermally insulating, in order to avoid cooling of the surroundings or heating of the cooling device.
  • the inner wall of the cooling space preferably consists of a thermally conductive material, such as metal, in order to improve the heat transfer from the inner cooling space to the coolant in the intermediate space.
  • the material of the inner wall not only has a good thermal conductivity, but also has a high specific heat capacity, so that the inner wall with its heat capacity as a thermal buffer counteracts unwanted temperature fluctuations ent.
  • the inner wall is substantially lattice-shaped, so that the coolant located in the intermediate space can outgas largely unhindered into the cooling space.
  • the cooling chamber is trough-shaped and has a peripheral edge on its upper side
  • the coolant supply line preferably has a coolant distributor which extends along the peripheral edge of the cooling space and the coolant over its length distributed in the space between the inner wall and the outer wall of the refrigerator initiates.
  • the coolant is thus introduced uniformly into the intermediate space between the inner wall and the outer wall of the cooling space, which advantageously leads to a uniform temperature distribution in the cooling space, since the cooling space is uniformly cooled from all sides.
  • a heating element is arranged in the cooling space in order to heat the cooling space or to thaw the refrigerated goods located in the cooling space.
  • this heating element is arranged below or in a heating plate, wherein the Schu ⁇ plate preferably has a plurality of passages, which allow a gas circulation.
  • the cooling device according to the invention it is also possible with the cooling device according to the invention to place a removable protective bell on the cooling space in order to avoid the penetration of moisture into the cooling space.
  • this protective bell is at least partially transparent in order to allow a visual inspection of the refrigerated goods located in the cold room.
  • the protective bell has a sample lock, through which the cooling medium can be introduced into the cooling space or removed from the cooling space, the sample lock largely preventing heat exchange with the environment.
  • a cold gas outlet can be arranged on the underside of the protective bell and / or on the upper side of the cooling space, via which coolant or cold gas can escape from the cooling space.
  • This cold gas outlet causes a large temperature gradient in the height of the cold gas outlet, wherein the temperature above the cold gas outlet is substantially higher than below the cold gas outlet. In this way, a fogging of the protective bell is advantageously prevented.
  • the cooling device preferably has one in which Refrigerator arranged temperature sensor to measure the temperature in the cold room or to regulate.
  • a controllable coolant valve is then preferably provided, which adjusts the quantity of the supplied coolant or the coolant flow.
  • the actual temperature control then takes place by means of a temperature regulator, which is connected on the input side to the temperature sensor and, on the output side, activates the coolant valve in accordance with a predetermined temperature setpoint.
  • the control of the coolant valve by the Temperatur ⁇ regulator can be done via a clock that alternately opens and closes the coolant valve, the opening and closing times of the coolant valve are set by the clock and set by the temperature controller.
  • the coolant is thus supplied in a discontinuous manner in that the coolant valve opens and closes alternately.
  • the temperature sensor for detecting the temperature in the cooling space is preferably arranged on the processing position of the cooling space in order to measure or regulate the optimum processing temperature in the cooling space.
  • the temperature controller therefore preferably regulates the temperature in the cold room so that no coolant lake forms at the bottom of the cold room.
  • the coolant is preferably liquid nitrogen, but the invention is not limited to nitrogen as the coolant, but can also be realized with other liquid or gaseous coolants which flow into the interspace. see the inner wall and the outer wall of the refrigerator can be initiated.
  • the cooling device according to the invention can be used for various temperature ranges, such as at temperatures of about -150 0 C, -130 0 C, -80 0 C, -40 0 C, + 4 ° C or +37 0 C, the above-mentioned Temperature ranges, for example, a range of +10 0 C, ⁇ 5 ° C or + 2 ° C can um ⁇ summarize.
  • a temperature of 37 0 C is advantageous because the growth temperature of biological cells is then optimal.
  • a temperature of +4 0 C offers the advantage that the physiological processes in the cells are slowed down. In a manipulation of cells at a temperature of less than 4 0 C cell damage is lower (eg with Tropsia and DMSO).
  • the invention encompasses not only the cooling device described above as a device, but also the use of such a cooling device for the examination, processing and / or manipulation of a cryoprobe.
  • FIG. 1 shows a perspective view of a preferred embodiment of the cooling device according to the invention with an attached protective bell
  • FIG. 2 shows a perspective view of the protective bell from FIG. 1 in the removed state
  • FIG. 3 shows a cross-sectional view of the wall structure of the cooling space in the cooling device from FIG. 1,
  • FIG. 4 shows a simplified perspective view of the coolant supply in the case of the cooling device from FIG. 1 and FIG.
  • FIG. 5 shows a control circuit equivalent circuit diagram of the cooling device from FIG. 1.
  • a cooling device 1 is used for tempering a cooling space for receiving cryoprobes in a Unter ⁇ search, manipulation and / or processing.
  • the cooling device 1 a cryogenic sump 2 with ei ⁇ nem trough-shaped, open-topped cooling chamber 3, wherein on the cryogenic sump 2, a removable protective bell 4 is placed, which prevents the ingress of moisture from the environment into the refrigerator and detailed in Figure 2 represents Darge.
  • the protective bell 4 has to introduce the cryoprobes into the cooling chamber 3 and to remove the cryoprobes from the cooling chamber 3 to a sample lock 5, which is attached to the side of the protective bell 4 and during insertion of the cryoprobes or when removing the cryoprobes heat exchange with the Environment largely prevented and minimizes the moisture in the refrigerator 3.
  • the protective bell 4 has on its upper side a lamp 6 in order to illuminate the cooling space 3 and thereby facilitate the manipulation of the cryoprobes located in the cooling space 3.
  • the protective bell 4 itself consists here of a transparent material, which allows a simple visual inspection by an operator.
  • two openings 9, through which cold gas can escape from the protective bell 4 are located at the back of the protective bell 4.
  • the result of the two openings 9 is that a high temperature gradient is established at the level of the two openings 9, since cold gas escapes outward from the two openings 9.
  • the atmosphere in the protective bell 4 above the openings 9 is therefore much warmer than below the openings 9, which counteracts fogging of the mecanican ⁇ de the protective bell 4.
  • cryotube 2 At the top of the cryotube 2 is located at the front on a control and display panel 10, where the temperature in the refrigerator 3 can be displayed and adjusted.
  • the cooling of the cooling chamber 3 takes place here by liquid nitrogen, which is supplied from a nitrogen tank (eg an Apollo tank) via a nitrogen line 11, where the nitrogen line 11 does not open directly into the cooling chamber 3, to the formation of a nitrogen lake on the ground of
  • a nitrogen tank eg an Apollo tank
  • a nitrogen line 11 where the nitrogen line 11 does not open directly into the cooling chamber 3, to the formation of a nitrogen lake on the ground of
  • the nitrogen line 11 opens via an electrically controllable coolant valve 12 into a coolant supply line 13, wherein thehariffen ⁇ supply line 13 along the peripheral edge of the trough-shaped Refrigerator 3 extends and releases the liquid nitrogen distributed over the length.
  • the refrigerator 3 is in this case of a metal,. lattice-shaped inner wall 14 bounded, which is enclosed by an outer wall 15, wherein the inner wall 14 and the outer wall 15 include a gap in which a buffer material 16 is arranged.
  • the coolant supply line 13 is arranged in the lateral direction between the inner wall 14 and the outer wall 15 above the buffer material 16 and has downwardly directed outlet openings through which liquid nitrogen is discharged from the interior of the coolant supply line 13 into the buffer material 16.
  • the buffer material 16 absorbs the liquid nitrogen and discharges it continuously through the grid-shaped inner wall 14 into the cooling space 3.
  • the coolant valve 12 operates in this case discontinuously by the coolant valve 12 either closes or opens.
  • the control of the coolant valve 12 takes place here by a clock generator 17, wherein the opening time T auF and the closing time T zü for the coolant valve 12 are set by a controller 18 to meter the coolant.
  • the regulation takes place as a function of the temperature in the cooling space 3, which is measured by a temperature sensor 19, wherein the temperature sensor 19 is arranged at the machining position of the cooling space 3.
  • the temperature sensor 19 therefore measures a temperature Ti ST and forwards it to a subtractor 20 which, as a further input variable, sets a nominal value T SOLL for the temperature in the cooling space 3 and calculates a target-actual deviation .DELTA.T calculated be ⁇ .
  • the controller 18 sets the opening time T aüF and the closing time T zü for Kuhlstoffventxl 12 so that the ge desired temperature (eg -130 0 C) prevails in the cooling chamber 3, without that at the bottom of the cooling chamber 3 a nitrogen lake forms.
  • the ge desired temperature eg -130 0 C

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif de refroidissement (1) pour refroidir un produit à refroidir, en particulier dans le cadre de l'analyse, de la manipulation et du traitement d'échantillons cryogéniques. Ce dispositif comprend une chambre de refroidissement (3) dans laquelle le produit à refroidir peut être placé, une paroi intérieure délimitant cette chambre de refroidissement (3), une paroi extérieure, un espace entre la paroi extérieure et la paroi intérieure, ainsi qu'une conduite d'agent de refroidissement (11) servant à acheminer un agent de refroidissement. Selon l'invention, la conduite d'agent de refroidissement (11) débouche dans l'espace entre la paroi intérieure et la paroi extérieure et achemine l'agent de refroidissement dans ledit espace, la paroi intérieure étant perméable à l'agent de refroidissement.
PCT/EP2005/003162 2004-07-19 2005-03-24 Dispositif de refroidissement pour echantillons biologiques WO2006007884A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/598,436 US20070169488A1 (en) 2004-07-19 2005-03-24 Cooling device for biological samples
DK05716364T DK1768782T3 (da) 2004-07-19 2005-03-24 Köleindretning til biologiske pröver
DE502005004582T DE502005004582D1 (de) 2004-07-19 2005-03-24 Kuhleinrichtung für biologische proben
EP05716364A EP1768782B1 (fr) 2004-07-19 2005-03-24 Dispositif de refroidissement pour echantillons biologiques
PL05716364T PL1768782T3 (pl) 2004-07-19 2005-03-24 Urządzenie chłodnicze dla próbek biologicznych

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004034827A DE102004034827A1 (de) 2004-07-19 2004-07-19 Kühleinrichtung für biologische Proben
DE102004034827.8 2004-07-19

Publications (1)

Publication Number Publication Date
WO2006007884A1 true WO2006007884A1 (fr) 2006-01-26

Family

ID=34962965

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/003162 WO2006007884A1 (fr) 2004-07-19 2005-03-24 Dispositif de refroidissement pour echantillons biologiques

Country Status (9)

Country Link
US (1) US20070169488A1 (fr)
EP (1) EP1768782B1 (fr)
AT (1) ATE399593T1 (fr)
DE (2) DE102004034827A1 (fr)
DK (1) DK1768782T3 (fr)
ES (1) ES2308455T3 (fr)
PL (1) PL1768782T3 (fr)
PT (1) PT1768782E (fr)
WO (1) WO2006007884A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120255313A1 (en) * 2011-04-06 2012-10-11 Igor Katkov Method And Scalable Devices For Hyper-Fast Cooling

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012024105A1 (de) 2012-12-10 2014-06-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Kryo-Lagereinrichtung und Verfahren zu deren Betrieb
FR3073277B1 (fr) * 2017-11-07 2020-06-12 Universite De Lorraine Systeme d'echangeur de chaleur a paroi anti-depot
KR102009505B1 (ko) * 2019-01-17 2019-08-12 주식회사 엘지화학 유전자 증폭 모듈
EP3863039A1 (fr) * 2020-02-04 2021-08-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Station de travail, station de préparation et procédé pour la manipulation d'un ensemble de grille de microscopie électronique
TWI795935B (zh) * 2021-10-04 2023-03-11 沃司科技股份有限公司 具有溫度控制功能的保冷箱及其溫度控制方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4455842A (en) * 1981-07-15 1984-06-26 Biotech Research Laboratories, Inc. Device and method for controlled freezing of cell cultures
US4680945A (en) * 1985-05-15 1987-07-21 Carl-Zeiss-Stiftung Cooling chamber for processing specimens for microscopic and electron-microscopic investigations
EP0301168A1 (fr) * 1987-07-27 1989-02-01 Cryogenics International, Inc. Dispositif et procédé pour le traitement cryogénique de matériaux
US5601143A (en) * 1994-02-25 1997-02-11 Binder; Peter M. Laboratory regrigerator, in particular a refrigerated incubator
US20030066639A1 (en) * 2001-10-09 2003-04-10 Jeng-Yau Wang Flexible and mobile high transition rate of temperature test devices
US20030140648A1 (en) * 2001-11-29 2003-07-31 Wataru Hattori Method and apparatus for adjusting device used at low temperature without deterioration thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3595030A (en) * 1969-08-12 1971-07-27 Donald J Roslonski Device for cooling bottled liquids
US3618336A (en) * 1970-05-21 1971-11-09 James R Palma Cooled coffin structure
CH660910A5 (de) * 1983-03-10 1987-05-29 Ernst Hartmann Verfahren zum durchfluten eines von einer wandung umgebenen raumes mit einem gas.
US4481779A (en) * 1983-06-22 1984-11-13 Union Carbide Corporation Cryogenic storage container
US5587228A (en) * 1985-02-05 1996-12-24 The Boeing Company Microparticle enhanced fibrous ceramics
US4566293A (en) * 1984-12-03 1986-01-28 The B. F. Goodrich Company Method of sample preparation and apparatus therefor
DE4012600C2 (de) * 1989-05-26 1998-05-14 Leica Ag Mikrotom
DE4116500A1 (de) * 1991-05-21 1992-11-26 Binder Wtb Labortech Gmbh Labor-waermeschrank
US5546756A (en) * 1995-02-08 1996-08-20 Eaton Corporation Controlling an electrically actuated refrigerant expansion valve
DE19600896A1 (de) * 1996-01-12 1997-07-17 Gerd Dr Schneider Regeleinrichtung mit stufenlos einstellbarem Dosierventil für kryogene verflüssigte Gase zur genauen Temperaturstabilisierung von gefrorenen Proben
US5976871A (en) * 1997-07-02 1999-11-02 Venturedyne, Ltd. Cytogenetic chamber
US6044648A (en) * 1997-09-19 2000-04-04 Forma Scientific, Inc. Cooling device having liquid refrigerant injection ring
US6658875B2 (en) * 2001-04-25 2003-12-09 Gsle Development Corporation Method and apparatus for temperature control in a refrigeration device
US6688132B2 (en) * 2001-06-06 2004-02-10 Nanopore, Inc. Cooling device and temperature-controlled shipping container using same
DE10332799B4 (de) * 2003-07-18 2007-03-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung und Verfahren zur Handhabung einer Probe

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4455842A (en) * 1981-07-15 1984-06-26 Biotech Research Laboratories, Inc. Device and method for controlled freezing of cell cultures
US4680945A (en) * 1985-05-15 1987-07-21 Carl-Zeiss-Stiftung Cooling chamber for processing specimens for microscopic and electron-microscopic investigations
EP0301168A1 (fr) * 1987-07-27 1989-02-01 Cryogenics International, Inc. Dispositif et procédé pour le traitement cryogénique de matériaux
US5601143A (en) * 1994-02-25 1997-02-11 Binder; Peter M. Laboratory regrigerator, in particular a refrigerated incubator
US20030066639A1 (en) * 2001-10-09 2003-04-10 Jeng-Yau Wang Flexible and mobile high transition rate of temperature test devices
US20030140648A1 (en) * 2001-11-29 2003-07-31 Wataru Hattori Method and apparatus for adjusting device used at low temperature without deterioration thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120255313A1 (en) * 2011-04-06 2012-10-11 Igor Katkov Method And Scalable Devices For Hyper-Fast Cooling
US9557090B2 (en) * 2011-04-06 2017-01-31 Celltronix Method and scalable devices for hyper-fast cooling

Also Published As

Publication number Publication date
DE102004034827A1 (de) 2006-03-16
US20070169488A1 (en) 2007-07-26
EP1768782A1 (fr) 2007-04-04
EP1768782B1 (fr) 2008-07-02
PL1768782T3 (pl) 2008-12-31
DE502005004582D1 (de) 2008-08-14
PT1768782E (pt) 2008-09-19
ATE399593T1 (de) 2008-07-15
DK1768782T3 (da) 2008-09-29
ES2308455T3 (es) 2008-12-01

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