WO2005032258A1 - 栽培植物中の硝酸塩含有量低減用組成物 - Google Patents
栽培植物中の硝酸塩含有量低減用組成物 Download PDFInfo
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- WO2005032258A1 WO2005032258A1 PCT/JP2004/014290 JP2004014290W WO2005032258A1 WO 2005032258 A1 WO2005032258 A1 WO 2005032258A1 JP 2004014290 W JP2004014290 W JP 2004014290W WO 2005032258 A1 WO2005032258 A1 WO 2005032258A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/002—Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/141—Feedstock
- Y02P20/145—Feedstock the feedstock being materials of biological origin
Definitions
- the present invention reduces the nitrate content in vegetables, feeds, and other agricultural products by using biologically active substances that do not substantially harm the environment in the agricultural field, particularly in the field of cultivated plant production. This relates to technology for improving the quality of agricultural products. Background art
- nitrate contained in foods has the potential to cause many metabolic disorders, such as harming human health and reducing blood hemoglobin content.
- This nitrate nitrogen is considered to be a substance that promotes the formation of mutagenic and carcinogenic ditorosamine in vivo.
- nitrate content is reduced in cultivated plant products, especially vegetables that are eaten unheated, and cultivated plants for livestock feed where there is a risk of nitrate contamination in milk and other livestock products.
- cultivated plant products especially vegetables that are eaten unheated, and cultivated plants for livestock feed where there is a risk of nitrate contamination in milk and other livestock products.
- nitrate is also the most important nitrogen source in most agricultural cultivated plants. Promotes plant growth and also acts as a nutrient and signal. Nitrate as a nutrient is reduced to ammonium salt, which is then converted to a component of amino acid, and then to protein, chlorophyll, hormones, and other components of plant nitrogenous compounds. Nitrate as a signal also modulates nitrogen and carbon metabolism by enhancing the expression of large groups of genes. Nitrate is reduced to ammonium salt in two stages. In the first stage, the reaction is promoted by nitrate reductase, and nitrate is reduced to nitrite. In the second stage, nitrite is reduced to ammonium salt by nitrite reductase. The incorporation of ammonium salts into amino acids is mainly catalyzed by glutamate synthase and glutamate synthase.
- compositions for reducing the nitrate nitrogen content in plants include those based on aqueous solutions of 5-aminolevulinic acid and its salts (Yoshida et al., 1991; US Pat. No. 489, 572) are known.
- the disadvantage of this composition is that it is expensive ($ 50,000 to $ 750,000 / kg) 5—Requires large amounts of aminolevulinic acid (from 80 g to 3 kg / ha). Yes, it raises the price of the composition itself and may not be economically viable.
- This composition has one more disadvantage. 5
- Monoaminolevulinic acid is a herbicide and, under certain conditions, may inhibit the growth of applied cultivated plants, even in small amounts.
- 5-aminolevulinic acid is to be used for treating soil and Z or plants.
- the growth-promoting effect extends to the weeds as well as the target plant.
- the prototype we adopted was a chitosan-based composition proposed to increase plant resistance to disease (Novodzilov et al., 2000, Russia Republic Patent No. 2185850).
- this composition does not substantially affect the nitrate nitrogen content in the cultivated plant product.
- Patent Document 1 Russian Patent No. 2, 158, 510
- Patent Document 2 U.S. Pat. No. 5,489,572 Disclosure of the invention
- An object of the present invention is to provide a drug capable of solving the above-mentioned disadvantages of the prior art.
- an object of the present invention is to develop a drug that reduces the amount of nitrate contained in a cultivated plant without requiring a transgenic plant or an expensive compound.
- Another object of the present invention is to provide a method for reducing the nitrate content in a cultivated plant by the agent.
- the present inventor has found that the use of a composition containing chitosan as an agent for reducing the nitrate content is extremely effective in solving the above problems, and has completed the present invention. . More specifically, the composition containing chitosan can efficiently reduce the nitrate content in cultivated plants by combining it with an organic acid, a nitrate reductase active substance and a chlorophyll synthesis promoter.
- the present invention includes, for example, the following items.
- a composition for reducing a nitrate content in a cultivated plant comprising a chitosan, an organic acid, a nitrate reductase active substance, and a chlorophyll synthesis promoting substance in the following mass ratio (%).
- composition for reducing a nitrate content according to any one of [1] to [4], wherein the organic acid is a combination of succinic acid, ascorbic acid and sorbic acid.
- the organic acid is a combination of succinic acid, ascorbic acid, and sorbic acid, and the ratio of succinic acid 2, ascorbic acid 0 to the total amount of organic acids contained is 0.
- Nitrate reductase active substances are derived from iron nitrate, ammonium molybdate, indole acetic acid, naphthyl acetic acid, ethylene diamine acetic acid, and N, N-dicarboxymethyl daltamic acid.
- the composition for reducing nitrate content according to any one of [1] to [6], wherein the composition is at least one member selected from the group consisting of:
- nitrate reductase active substance is a combination of iron nitrate, ammonium molybdate, indoleacetic acid, and ethylenediaminetetraacetic acid.
- the composition for reducing a nitrate content according to any one of the above.
- the nitrate reductase active substance is a combination of iron nitrate, ammonium molybdate, indole acetic acid, and ethylene diamine tetraacetic acid, and the total amount of the nitrate reductase active substance contained in the ratio Mass ratio to iron nitrate 20; ammonium molybdate 4 to 6, indoleacetic acid 0.5 to 1.5, ethylenediaminetetraacetic acid 0.5 to 1.5.
- a composition for reducing nitrate content is a combination of iron nitrate, ammonium molybdate, indole acetic acid, and ethylene diamine tetraacetic acid, and the total amount of the nitrate reductase active substance contained in the ratio Mass ratio to iron nitrate 20; ammonium molybdate 4 to 6, indoleacetic acid 0.5 to 1.5, ethylenediaminetetraacetic acid 0.5 to 1.5.
- the nitrate reductase active substance is a combination of iron nitrate, ammonium molybdate, naphthylacetic acid and N, N-dicarboxymethyldaltamic acid. 7] The composition for reducing a nitrate content according to any one of the above.
- the nitrate reductase active substance is a combination of iron nitrate, ammonium molybdate, naphthylacetic acid, and N, N-dicarboxymethyldanoletamate, and the nitrate reductase contained in the ratio is used.
- the composition for reducing a nitrate content according to [10] which is in a range of from 1.5 to 1.5.
- the chlorophyll synthesis promoting substance is at least one selected from the group consisting of 2-oxodaltalic acid and L-glutamic acid [1] to [11]. ]
- the composition for reducing a nitrate content according to any one of the above items.
- the chlorophyll synthesis-promoting substance is a combination of 2-otasoglutaric acid and L-glutamic acid, and the proportion thereof is 2-octanol in mass ratio to the entire chlorophyll synthesis-promoting substance contained.
- composition for reducing nitrate content which is characterized by further containing a surfactant in an amount of 1 to 3 parts by mass with respect to 100 parts by mass of [1] to [1]. 4] The composition for reducing a nitrate content according to any one of the above. [16] The composition for reducing a nitrate content according to [15], wherein the surfactant is polyoxyethylene sorbitan monostearate.
- the nitrate content-reducing composition according to any of [1] to [16] is sprayed on the leaves of the cultivated plant to reduce the nitrate content in the cultivated plant. How to reduce.
- the cultivated plant is at least one selected from the group consisting of spinach, lettuce, cabbage, indone, parsley, radish, onion, beet, carrot, and potato.
- Chitosan in the present invention is not particularly limited. Any form and property that is generally called chitosan can be used.
- chitosan is, for example, a natural polysaccharide of chitin represented by the following formula (1), also known as] 3-poly-N-acetyl-D-darcosamine; Is a product obtained by deacetylating a hydroxyl group by hydrolysis to form an amino group, and examples thereof include / 3-poly-D-dalcosamine represented by the following formula (2).
- acetamido groups When deacetylating chitin to produce chitosan, it is not essential that strictly all acetamido groups be hydrolyzed to amino groups. Some acetamido groups may remain as long as there is no problem in using them as the chitosan of the present invention. Of course, it may be one in which the acetamido group is not detected or substantially absent. In the present invention, it is defined as "chitosan" including those in which some acetamido groups remain.
- the ratio of amino groups to acetamido groups present in a chitosan molecule is generally called "degree of deacetylation" and is defined by the following equation. The measurement method will be described later.
- Deacetylation degree (number of amino groups / (several dozens of amino groups)) X I 0 0 (%)
- composition of the present invention does not depend on the degree of deacetylation, it is generally preferable to use chitosan having a degree of deacetylation of 70% or more.
- the degree of deacetylation is more preferably in the range of 75% to 95%, most preferably in the range of 75% to 90%.
- the molecular weight of chitosan discussed in the present invention refers to the mass average molecular weight, and the measuring method thereof will be described later.
- the present invention proposes the use of chitosan having a molecular weight as defined above of 20 to 180 kDa for adjusting the composition. This corresponds to a degree of polymerization of from 120 to 120, but it is preferable to use chitosan having a molecular weight of from 20 to 120 kDa if possible.
- Such a composition containing chitosan having a molecular weight is preferable because it is easily dissolved in water and permeates into plants quickly.
- the molecular weight of chitosan is more preferably in the range of 20 to 100 kDa, most preferably in the range of 20 to 70 kDa.
- the origin of chitosan usable in the present invention is not particularly limited. That is, not only those obtained by deacetylating naturally-derived chitin using a chemical or biochemical technique, but also those extracted from the cell wall of fungi can be used.
- the ratio of chitosan in the composition for reducing nitrate content is in the range of 15% to 25% by mass. This chitosan ratio is preferably in the range of 15% to 20%, more preferably in the range of 18% to 20%.
- the basis of the ratio (mass ratio) of each component such as chitosan is the total mass of the composition for reducing a nitrate content including the component itself.
- the mass of chitosan is A gram and the total mass of components other than chitosan is B gram, the total mass of the composition for reducing nitrate content is (A + B) gram, and the mass of chitosan is The ratio is expressed as ⁇ AZ (A + B) ⁇ XI 00 (%) The
- composition for reducing a nitrate content of the present invention is characterized by containing, in addition to chitosan, an organic acid, a nitrate reductase active substance and a chlorophyll synthesis promoting substance.
- chitosan an organic acid
- a nitrate reductase active substance an organic acid
- chlorophyll synthesis promoting substance a chlorophyll synthesis promoting substance
- the nitrate reductase active substance that can be used in the present invention is not particularly limited as long as it can be used for agriculture or plant cultivation. here,
- Nirate reductase active substance refers to a substance having an action of increasing the activity of nitrate reductase in a plant.
- the mechanism of action of the nitrate reductase is not particularly limited as long as the action of enhancing the activity of the nitrate reductase can be exerted as a result.
- this nitrate reductase active substance is composed of iron nitrate, ammonium molybdate, indole acetic acid, naphthyl acetic acid, and ethylene diamine vinegar. At least one selected from the group consisting of acids and N, N-dicarboxymethylglutamic acid can be suitably used.
- nitrate reductase active substances From the viewpoint of the activity on the plant when the present composition is dissolved in water and applied to a cultivated plant, a combination of a plurality of nitrate reductase active substances is preferred.
- a combination of iron nitrate, ammonium molybdate, indoleacetic acid (or naphthylacetic acid), and ethylenediaminetetraacetic acid (or N, N-dicarboxymethylglutamic acid) is preferred.
- the proportion of the nitrate reductase active substance in the composition for reducing a nitrate content is in the range of 25% to 30% by mass. It is preferably in the range of 27% to 30%, more preferably in the range of 27% to 28%.
- iron nitrate, ammonium molybdate, indole acetic acid (or naphthyl acetic acid), and ethylenediamintetra acetic acid (or N, N-dicarboxymethylglutamic acid) there is no particular limitation on each ratio.
- iron nitrate, ammonium molybdate, indoleacetic acid (or naphthylacetic acid), and ethylenediaminetetraacetic acid (or N , N-dicarboxymethylglutamic acid is preferably in the range of 20: 4 to 6: 0.5 to: L.5: 0.5 to 1.5, more preferably 20 to 4.5. : 5: 1: 1.
- the nitrate reductase active substance has the function of simultaneously activating nitrite reductase in plants.
- the chlorophyll synthesis promoter that can be used in the present invention is not particularly limited as long as it can be used for agricultural or plant cultivation.
- the mechanism of action is not particularly limited as long as the action of promoting chlorophyll synthesis can be expressed as a result.
- Formula details of such a chlorophyll synthesis promoting substance if necessary, refer to “Safety and sustainable Aiming for Agriculture: Theory and Practice of Chitosan's Agricultural Use ”published on January 6, 2000 (1st edition, 1st print) (especially the description on page 39) can be referred to.
- the chlorophyll synthesis promoting substance is at least selected from the group consisting of 2-oxodaltalic acid and L-glutamic acid. More than one type It can be used for
- the proportion of the chlorophyll synthesis promoter in the composition for reducing nitrate content is in the range of 29% to 35% by mass. It is preferably in the range of 31% to 34%, more preferably in the range of 31% to 32%.
- the ratio of each is not particularly limited.
- the mass ratio of 2-oxodaltalic acid and L-daltamic acid is preferably in the range of 30: 0.5 to 1.5, and more preferably. Or 30: 1.
- Organic acids that can be used in the present invention are not particularly limited as long as they can be used for agricultural or plant cultivation.
- at least one organic acid selected from the group consisting of succinic acid, ascorbic acid and sorbic acid is preferably used. It is possible.
- a combination of a plurality of organic acids is preferred from the viewpoint of the activity on the plant when the composition is dissolved in water and applied to a cultivated plant.
- a combination of succinic, ascorbic, and sorbic acids is preferred.
- the proportion of the organic acid in the composition for reducing nitrate content is in the range of 15% to 25% by mass. It is preferably in the range of 18% to 25%, more preferably in the range of 20% to 25%.
- the mass ratio of succinic acid, ascorbic acid, and sorbic acid is 2: 0.8 to: 1.2: 0.8 to 1.8.
- a range of 2 is preferred, and a more preferred range is 2: 1: 1.
- the composition of the present invention may optionally include a surfactant to increase the retention of the composition on the leaf surface of the plant.
- a surfactant to increase the retention of the composition on the leaf surface of the plant.
- the chlorophyll synthesis promoting substance that can be used in the present invention is not particularly limited as long as it can be used for agriculture or plant cultivation.
- surfactants please refer to the JA Agricultural Fertilizer Agricultural Chemicals Department, Agrochemical Technology Promotion Division, “Kumi Eye Agrochemicals General Directory” (2001), as needed. It is possible to refer to the description issued on January 31 (especially, spreading agent, description on pages 1592-1601).
- the surfactant is preferably polyoxyethylene sorbitan monostearate.
- Kao Corporation (trade name: Leo Doll Super TW-S120) can be purchased and used.
- the ratio of the surfactant in the nitrate content reducing composition is in the range of 1% to 3% by mass. Preferably, it is in the range of 1.5% to 2.5%, more preferably in the range of 1% to 2%.
- the present invention also provides a method for using the above composition as an aqueous solution.
- the composition for reducing the content of nitrate in the cultivated plant for example, the composition for reducing the content of nitrate is dissolved in water having a pH of 5 to 9, and the pH of the aqueous solution is adjusted to 4.5 to 5.5. Then, this aqueous solution is sprayed on the seeds of agricultural products, or a method of immersing the seeds in the aqueous solution and a method of spraying the aqueous solution on the leaves of the growing plants. (Comparison with existing technology level)
- the composition proposed in the present invention can be applied to any kind of plant.
- the effect of the composition of the present invention is particularly remarkable in a plant having a high nitrate content level (the nitrate nitrogen in the total nitrogen contained in the commercialized portion of the plant is 0.5% or more).
- Similar plants include, for example, a series of vegetables (spinach, various lettuce, cabbage, indian, parsley, radish, onion) and various root vegetables (beet, carrots, potatoes). and so on.
- composition of the present invention are based on a previously unexplained portion of chitosan, a bioactive substance that is substantially non-toxic and does not harm the environment.
- this composition In combination with other components contained in this composition, it has the advantage of activating nitrate reductase and nitrite reductase, increasing chlorophyll in plants, and further increasing osmotic activity, This will increase the assimilation of inorganic nitrogen and reduce nitric acid in agricultural products. It is possible to reduce the salt content.
- Chitosan can exert the above effects only by specific combinations, including only a limited number of organic acids, and only some of the compounds we have filed. The reason for this is presumed to be that the effect of this composition that reduces the nitrate content is due to the following three effects.
- the precursor of chlorophyll synthesis is 2-oxodaltalic acid
- L-glutamic acid enhances chlorophyll in plant leaves, thereby enhancing photosynthesis and sugar formation. At this time, sugars lead to nitrogen assimilation in plants.
- the osmotic activity of chitosan and the other components of the composition results in the prevention of nitrate reduction to the end product, which is the reduced form of nitrogen, and synchronizes carbohydrate and nitrogen metabolism.
- the composition of the present invention is water-soluble, has excellent film-forming ability and adhesion ability, and has high adhesiveness to plant substances.
- the composition has activity and stability, for example, in a nearly neutral aqueous solution having a pH of 4.5 to 5.5.
- the compositions of the present invention have no phytotoxicity at the concentrations used.
- Chitosan, 2-oxoglutaric acid, L-glutamic acid, succinic acid, sorbic acid, ascorbic acid, and indoleacetic acid that can be used in the present invention are the active substances in the present application.
- this chitosan-based composition is used to reduce the content of non-assimilated nitrates, the amount and frequency of use may vary depending on environmental conditions. Normally, a method of treating seeds with the present composition and spraying an aqueous solution on plants in a growing stage can be adopted. For example, spraying of an aqueous solution on growing plants may be performed once a week for three weeks until harvesting. Under conditions of lack of light or other stress, the frequency of spraying may be increased as appropriate.
- the seed-stage treatment of vegetables can be performed, for example, by immersing in a 0.5% aqueous solution using the present composition in a dry state.
- the amount of the solution used is preferably, for example, about 1 liter per 1 kg of seed.
- a 0.5% aqueous solution using a dry powder can be used in an amount of 200 to 300 liters per lha.
- composition (part 1) Chitosan with a molecular weight of 50 kDa 120, nitrate reductase active substance 27, chlorophyll synthesis promoter 31, organic acid 120, surface activity Agent 1
- composition (part 2) (wt%): chitosan-15 with a molecular weight of 170 kDa, nitrate reductase active substance 25, chlorophyll synthesis promoter 34, organic acid 25, interface Activator 1
- Table 1 shows the data for use example 1. As shown in this data, higher nitrate reductase activity than control persisted for 7 days after application to lettuce. For the purpose of activating nitrate reductase in leaves of lettuce (cultivar name “Azart”)
- Example 1 shows the effect of the present composition to improve the chlorophyll content of lettuce.
- the composition of the composition is as shown in Use Example 1.
- Table 2 the pending composition was confirmed to increase the chlorophyll content over three days after the application to the lettuce.
- composition reduces the nitrate content in the leaves of various lettuce (variety name “Azart”, “Laura 'Rossa”, “fringed leaves of Odessa”) and spinach (variety name “matador”) Show action.
- the components of composition (part 1) are the same as those in use examples 1 and 2.
- the components (wt%) of composition (part 3) are: chitosan 18 with a molecular weight of 100 kDa, and a nitrate reductase active substance 13 0, chlorophyll synthesis promoter-31, organic acid-18, surfactant-13.
- the composition proposed in the present invention significantly reduces the nitrate content in leaves and spinach leaves grown to the commodity level. This was supported.
- the composition of the present invention can reduce the nitrate content in vegetables and root vegetables by adding a small amount of a bioactive substance to chitosan.
- the use of this composition improves the efficiency of use of nitrogen fertilizers, and as a result, reduces environmental pollution (particularly pollution in water bodies) due to nitrate, which is highly toxic to the fauna, and reduces environmental impact. It is possible to harvest agricultural products with low nitrate content that do not cause harm.
- Og is accurately collected in a titration vessel, 50 ml of water and 0.2 ml of toluidine blue (indicator) sample solution are added, mixed well, and then the polyvinyl chloride solution is added. Titrate with. The end point is the point at which blue changes to magenta.
- the titer is V ml.
- the free amino group mass (X) (corresponding to the mass of dalcosamine residues) and the bound amino group mass (Y) (corresponding to the mass of N-acetyl dalcosamine residues) in chitosan are as follows:
- Deacetylation degree (%) (X / 16 1) / (X / 16 1 + Y / 203) X 100
- 161 is the equivalent molecular weight of the dalcosamine residue
- 203 is the equivalent molecular weight of the ⁇ -acetyl dalcosamine residue.
- the specific viscosity is measured using an Ostwald viscometer and determined using the conversion table in Table 6 below.
- To measure the viscosity add 50 ml of a 4% acetic acid aqueous solution and 50 ml of a 0.6 M saline solution to 50 mg of the chitosan sample, and dissolve. Disassemble and prepare. Using a capillary tube with an inner diameter of 0.5 mm of Shiwata's Ostwald viscometer, measure the transit time from engraved line a to engraved line b, and let this time be t.
- the molecular weight of chitosan can also be determined by gel permeation chromatography (GPC).
- Analytical instruments used for the GPC measurement method include a differential refractive index detector (Shodex RI —71), a pump (ShodexDS—4), and a data processing unit GPC software (Waters Maxima)
- 0.1 M acetate buffer (0.1 M acetic acid, 750 ml, 0.1 M sodium acetate, 100 ml) as eluent, and further add 0.1 M sodium acetate.
- the solution whose pH has been adjusted to 4 is filtered through a filter with a pore size of 0.45 ⁇ ) and passed at a flow rate of 1.
- Oml Zmin Dissolve the sample in the eluent 48 hours before the GPC measurement so that the sample concentration is 0.1% by mass. Do not vigorously shake the solution or use ultrasound when dissolving. Inject 200 ⁇ 1 of the sample solution prepared in this way.
- chitosan having a known molecular weight determined by the above “viscosity measurement method” is used.
- Figure 1 shows the GPC chromatogram measured under the above conditions.
- a composition for reducing a nitrate content in a cultivated plant comprising chitosan, an organic acid, a nitrate reductase active substance and a chlorophyll synthesis promoting substance at a predetermined mass ratio.
- the amount of nitrate contained in a cultivated plant can be reduced without requiring a transgenic plant or an expensive compound.
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JP2005514441A JPWO2005032258A1 (ja) | 2003-10-06 | 2004-09-22 | 栽培植物中の硝酸塩含有量低減用組成物 |
EP04773467A EP1671543A1 (en) | 2003-10-06 | 2004-09-22 | Composition for lowering nitrate content in domesticated plant |
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RU2003129759/04A RU2257711C2 (ru) | 2003-10-06 | 2003-10-06 | Композиция на основе хитозана, снижающая содержание нитратов в растениеводческой продукции |
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ES2602356T3 (es) * | 2007-01-09 | 2017-02-20 | Novozymes Biologicals Holding A/S | Composiciones combinadas de lipo-quitooligosacáridos para un crecimiento y rendimiento mejorado de plantas |
EP2167652A1 (en) * | 2007-06-15 | 2010-03-31 | Pioneer Hi-Bred International Inc. | Nitrate reductases from porphyra, compositions and methods of use thereof |
CN103563642B (zh) * | 2013-11-22 | 2014-12-03 | 湖南省农业生物资源利用研究所 | 一种低硝酸盐含量茄子的栽培方法 |
CN104145801A (zh) * | 2014-09-10 | 2014-11-19 | 扬州大学 | 一种降低叶菜类体内硝酸盐含量的活体蔬菜栽培方法 |
CN105601429A (zh) * | 2016-01-12 | 2016-05-25 | 威海索尔生物科技有限公司 | 纯天然植物杀虫营养剂及其在蔬菜种植中的应用 |
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JP2001316204A (ja) * | 2000-04-28 | 2001-11-13 | Kao Corp | 植物活力剤 |
JP2001340025A (ja) * | 2000-03-29 | 2001-12-11 | ▲吉▼田 勲 | 植物成分調整剤およびこれを用いた植物用葉面散布剤 |
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JPS5634565B2 (ja) * | 1974-06-07 | 1981-08-11 | ||
US4069249A (en) * | 1975-11-11 | 1978-01-17 | W. R. Grace & Co. | N,N-di-(hydroxybenzyl)-trimethylene diaminediacetic acids |
US4340765A (en) * | 1980-08-14 | 1982-07-20 | Gray Gary M | 4-Phenoxy-2-butene derivatives as plant growth regulators |
JPH10218707A (ja) * | 1997-02-13 | 1998-08-18 | Lion Corp | 土壌・植物散布液組成物 |
JP2986753B2 (ja) * | 1997-02-25 | 1999-12-06 | 高松油脂株式会社 | 魚類棲息地域用植物有害生物防除剤 |
US20040035162A1 (en) * | 1999-02-05 | 2004-02-26 | Williams Richard Henry | Fertiliser |
-
2003
- 2003-10-06 RU RU2003129759/04A patent/RU2257711C2/ru active
-
2004
- 2004-09-22 JP JP2005514441A patent/JPWO2005032258A1/ja active Pending
- 2004-09-22 CN CNA2004800290352A patent/CN1863461A/zh active Pending
- 2004-09-22 EP EP04773467A patent/EP1671543A1/en not_active Withdrawn
- 2004-09-22 WO PCT/JP2004/014290 patent/WO2005032258A1/ja active Application Filing
- 2004-09-23 US US10/947,302 patent/US20050148472A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2001340025A (ja) * | 2000-03-29 | 2001-12-11 | ▲吉▼田 勲 | 植物成分調整剤およびこれを用いた植物用葉面散布剤 |
JP2001316204A (ja) * | 2000-04-28 | 2001-11-13 | Kao Corp | 植物活力剤 |
Non-Patent Citations (1)
Title |
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DOKLADY AKADEMII NAUK UKRAINSKOI SSR, SERYIA B: GEOLOGICHESKIE, KHIMICHESKIE I BIOLOGICHESKIE NAUKI, no. 2, 1990, pages 74 - 76, XP002987085 * |
Also Published As
Publication number | Publication date |
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JPWO2005032258A1 (ja) | 2006-12-14 |
CN1863461A (zh) | 2006-11-15 |
RU2003129759A (ru) | 2005-03-27 |
EP1671543A1 (en) | 2006-06-21 |
US20050148472A1 (en) | 2005-07-07 |
RU2257711C2 (ru) | 2005-08-10 |
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