WO2005019406A1 - インキュベータ用収容容器及びインキュベータ - Google Patents
インキュベータ用収容容器及びインキュベータ Download PDFInfo
- Publication number
- WO2005019406A1 WO2005019406A1 PCT/JP2004/012005 JP2004012005W WO2005019406A1 WO 2005019406 A1 WO2005019406 A1 WO 2005019406A1 JP 2004012005 W JP2004012005 W JP 2004012005W WO 2005019406 A1 WO2005019406 A1 WO 2005019406A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- incubator
- container
- storage container
- photographing
- cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
Definitions
- the present invention relates to an incubator container and an incubator.
- incubators that can maintain a constant environment such as internal temperature and humidity have been widely used for culturing cells.
- a rectangular plate-like opening / closing door is attached to the front opening of a rectangular box-shaped incubator body so as to be openable and closable, and a heat exchanger or a humidifier for keeping the internal environment constant in the incubator body.
- a conditioner such as a vessel was provided.
- the present invention relates to a storage container for an incubator arranged inside an incubator, wherein a ventilation device for ventilating the inside is connected and connected.
- the container for the incubator contains a device for photographing the culture state of cells therein.
- the incubator container contains a device for stimulating cells therein.
- a hollow support tube for supporting the incubator housing container is provided inside the incubator, and an air supply pipe and an exhaust air that connect and connect the incubator housing container and the ventilation device.
- the tube is housed inside the support tube.
- the support tube has flexibility and can change and fix the posture of the incubator container.
- the present invention provides an incubator provided with an opening / closing door in the incubator body, a photographing means for photographing a culture state of cells inside the incubator body, and an image processing means for processing an image photographed by the photographing means.
- the display means for displaying the culture state of the cells photographed by the photographing means is provided in front of the opening / closing door, and the container for the incubator provided inside the incubator is provided. It houses a photographing means inside and a ventilation device for ventilating the inside of the incubator container.
- the display means is configured to display a cell culture state on an operation panel provided on a front surface of the opening and closing door.
- FIG. 1 is a schematic diagram showing a configuration of an incubator.
- FIG. 2 is a front view of an incubator.
- FIG. 3 is a perspective view of a storage container for an incubator.
- a rectangular plate-shaped opening / closing door is openably and closably attached to a front opening of a rectangular box-shaped incubator body having a cell culture chamber formed therein. It has a built-in conditioner such as a heat exchanger and humidifier to keep the environment constant.
- the incubator body is provided with a photographing means for photographing a cell culture state. And an image processing means for processing an image photographed by the photographing means.
- the image processing means can be installed outside the incubator.
- the photographing means attaches the base end of a hollow cylindrical support tube to the cell culture chamber of the incubator main body, and attaches a substantially cylindrical closed container to the tip end of the support tube.
- the photographing device is housed inside the housing container.
- various devices such as a vibrating device, an irradiating device, or a stirrer for giving a stimulus such as vibration or light to the cells may be stored in the container.
- the photographing means communicates and connects the ventilation device disposed inside the incubator main body to the housing container, and also connects and connects the cord connecting the photographing means and the image processing means and the ventilation device to the housing container.
- the supply pipe and the exhaust pipe are housed inside the support pipe.
- the support tube has flexibility so that the posture of the imaging device can be changed and fixed.
- the opening / closing door is provided with a display means for displaying the culture state of the cells photographed by the photographing means on the front surface, and this display means is provided by an operation panel attached to the front surface of the opening / closing door. Display the cell culture status.
- the incubator is provided with the photographing means for photographing the culture state of the cells and the image processing means for processing the image photographed by the photographing means inside the incubator body,
- the photographing means for photographing the culture state of the cells
- the image processing means for processing the image photographed by the photographing means inside the incubator body
- the incubator displays the culture state of the cells on the operation panel arranged in front of the opening / closing door
- the operation panel can be used as both a display member and an operation member. It is possible to prevent an increase in the number of components that make up the system and an increase in manufacturing costs.
- the incubator houses various devices such as a photographing device, a vibration device, and an irradiation device. Since the container is housed inside the container and a ventilator is connected to this container, the ventilator keeps the temperature and humidity inside the container almost constant, so that the inside of the incubator body can be used for various devices. Even when the temperature and humidity exceed the limits, the inside of the container can be kept at the temperature and humidity within the specifications of the various devices, preventing the failure of various devices before they occur.
- the culture can be performed while taking pictures of the culture conditions and stimulating the cells.
- a hollow support tube is provided in the incubator main body, a storage container is continuously provided at the tip of the support tube, and various devices in the storage container are connected to devices outside the storage container.
- the supply and exhaust pipes that connect and connect the cords and the container to the ventilation system are housed inside the support pipes, so that the inside of the incubator can be organized and these cords and supply and exhaust pipes can be used. The ability to prevent accidental contact with cells in culture.
- the incubator allows the posture of various devices to be changed and fixed by using a flexible support tube, by changing the posture of each device using the support tube and then fixing it, Various devices can be set at any angle.
- a rectangular plate-shaped opening / closing door 3 is attached to a rectangular box-shaped incubator body 2 via hinges 4 and 4 so as to be freely opened and closed.
- the incubator main body 2 has a rectangular box-shaped space-like cell culture room 5 formed therein, and a device accommodation room 6 for accommodating various devices around the cell culture room 5. I have.
- the cell culture chamber 5 has a rectangular plate-shaped stationary table 7 mounted inside, so that a petri dish 8 for culturing cells can be left standing on the stationary table 7, and a heat insulating material is provided around the cell. 9 is attached so that the front opening can be sealed with the door 3.
- the cell culture room 5 is connected to a heat exchanger and a humidifier (conditioner) housed and disposed in the device housing room 6 so that the environment such as the temperature and humidity inside can always be kept constant. .
- the cell culture chamber 5 is provided with an imaging means 10 for imaging the culture state of the cells inside.
- the imaging means 10 is configured such that base ends of hollow cylindrical support tubes 11, 11 are attached to the upper wall and the lower wall of the cell culture chamber 5, and a substantially cylindrical support tube 11, 11 is attached to a distal end of the support tubes 11, 11.
- the accommodation container 12 is attached, and a photographing device (CCD camera) 13 is attached to the inside of the accommodation container 12 via a support 14 as shown in FIG.
- the support 14 may have a structure that can move in three axes (XYZ axes). Further, various devices such as a vibration device, an irradiation device, and a stirring device for stimulating cells together with the imaging device 13 or alone can be stored inside the storage container 12.
- a transparent glass 16 is attached to a front opening of a hollow cylindrical container body 15, and a disc-shaped rear wall 17 is attached to a rear opening of the container body 15.
- the cord 18 of the photographing device 13 passes through the wall 17, and the distal end of the support tube 11 is attached to the back of the rear wall 17 at substantially the center.
- a ventilation device 19 provided in the device storage chamber 6 is connected to the storage container 12 in communication therewith. That is, the storage container 12 is provided with an air supply port 20 and an exhaust port 21 substantially at the center of the rear wall 17, and an air supply pipe for connecting the air supply port 20 and the exhaust port 21 to the ventilation device 19. 22 and the end of exhaust pipe 23 are attached.
- the air supply pipe 22 and the exhaust pipe 23 are housed inside the support pipe 11 together with the cord 18.
- the support tube 11 is made of a flexible pipe that has flexibility, is deformable, and can maintain the shape after deformation. Thus, by deforming the support tube 11, the posture of the photographing device 13 inside the accommodation container 12 attached to the tip can be changed, and the posture after the change can be fixed. I have.
- the support tube 11 is connected at its base end to through holes 24, 24 formed in the upper and lower walls of the cell culture chamber 5.
- an image processing means 25 for processing an image photographed by the photographing means 10 and a control means 26 are provided above the cell culture chamber 5, and the rear of the cell culture chamber 5 is provided. Ventilation system 19 is installed.
- the image processing means 25 connects the code 18 of the photographing device 13 to the input terminals 28 and 28 of the image processing device 27, while connecting the control means 26 to the control terminal 29 of the image processing device 27.
- the cord 18 is covered with a flexible pipe 30 interposed between the through hole 24 of the cell culture chamber 5 and the input terminal 28.
- the control means 26 connects the control terminal 32 of the image processing device 27 to the control terminal 32 of the control device 31 via a control cord 33, and connects the display means 35 described later to the input / output terminal 34 of the control device 31. Connected.
- the base end of the air supply pipe 22 is attached to the air supply port 36, and the base end of the exhaust pipe 23 is attached to the exhaust port 37. Cover the end with a flexible pipe 30.
- the ventilation device 19 circulates the air inside the storage container 12 using the air supply pipe 22 and the exhaust pipe 23 to supply air at a predetermined temperature and a predetermined humidity into the storage container 12.
- the inside of the storage container 12 is set to a predetermined temperature and a predetermined humidity.
- the opening / closing door 3 has a touch panel-type operation panel 38 mounted at the upper center on the front surface.
- the input / output terminals 39 of the operation panel 38 are connected to the input / output terminals 34 of the control device 31 via input / output cords 40. Connected.
- the operation panel 38 is switched to the force photographing mode in which operation buttons for setting the temperature, humidity, and the like inside the cell culture chamber 5 are displayed, and the photographing is performed by the photographing means 10. An image is displayed, and functions as display means 35.
- the incubator 1 controls the photographing unit 10, the image processing unit 25, and the display unit 35 by the control unit 26, and displays the culture state of the cells photographed by the photographing unit 10 by the image processing unit 25. After the processing, the display means 35 displays.
- various devices such as a photographing device for photographing the culture state of the cells without affecting the environment inside the incubator, a vibrating device for stimulating the cells, and an irradiation device are provided inside the incubator. Can be accommodated.
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003-298942 | 2003-08-22 | ||
JP2003298942A JP4478415B2 (ja) | 2003-08-22 | 2003-08-22 | インキュベータ |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2005019406A1 true WO2005019406A1 (ja) | 2005-03-03 |
Family
ID=34213737
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2004/012005 WO2005019406A1 (ja) | 2003-08-22 | 2004-08-20 | インキュベータ用収容容器及びインキュベータ |
Country Status (2)
Country | Link |
---|---|
JP (1) | JP4478415B2 (ja) |
WO (1) | WO2005019406A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7128030B2 (ja) * | 2018-05-21 | 2022-08-30 | エスペック株式会社 | 環境形成装置 |
CN109321430B (zh) * | 2018-11-14 | 2022-04-29 | 王奕童 | 一种多环境微生物培养箱 |
CN111518689B (zh) * | 2020-04-15 | 2023-01-24 | 山东科技大学 | 一种智能菌落计数培养箱及计数方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05184347A (ja) * | 1992-01-13 | 1993-07-27 | Dainippon Ink & Chem Inc | 藻類の培養装置及び培養方法 |
JPH08287261A (ja) * | 1995-11-27 | 1996-11-01 | Hitachi Ltd | 画像認識システム及び画像認識制御システム |
JP2001231542A (ja) * | 2000-02-22 | 2001-08-28 | Hideo Tanaka | 振盪培養容器及び振盪培養方法 |
JP2003235540A (ja) * | 2002-02-20 | 2003-08-26 | Masahito Taya | 剥離細胞選別装置、剥離細胞選別方法及びそのプログラム |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58155087A (ja) * | 1982-03-12 | 1983-09-14 | Olympus Optical Co Ltd | 細胞の自動培養装置 |
JPS59214423A (ja) * | 1983-05-19 | 1984-12-04 | オリンパス光学工業株式会社 | 内視鏡用乾燥装置 |
JPH03259078A (ja) * | 1990-03-06 | 1991-11-19 | Tokimec Inc | 最適培養条件自動検出装置及び培養装置 |
JP3653903B2 (ja) * | 1996-12-10 | 2005-06-02 | フジノン株式会社 | 内視鏡の観察装置 |
JP4402249B2 (ja) * | 2000-03-31 | 2010-01-20 | 正仁 田谷 | 細胞培養方法、細胞培養装置及び記録媒体 |
US7015031B2 (en) * | 2002-01-24 | 2006-03-21 | Genx International, Inc. | Biological specimen-culturing system and method with onboard specimen development sensors |
-
2003
- 2003-08-22 JP JP2003298942A patent/JP4478415B2/ja not_active Expired - Lifetime
-
2004
- 2004-08-20 WO PCT/JP2004/012005 patent/WO2005019406A1/ja active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05184347A (ja) * | 1992-01-13 | 1993-07-27 | Dainippon Ink & Chem Inc | 藻類の培養装置及び培養方法 |
JPH08287261A (ja) * | 1995-11-27 | 1996-11-01 | Hitachi Ltd | 画像認識システム及び画像認識制御システム |
JP2001231542A (ja) * | 2000-02-22 | 2001-08-28 | Hideo Tanaka | 振盪培養容器及び振盪培養方法 |
JP2003235540A (ja) * | 2002-02-20 | 2003-08-26 | Masahito Taya | 剥離細胞選別装置、剥離細胞選別方法及びそのプログラム |
Also Published As
Publication number | Publication date |
---|---|
JP2005065567A (ja) | 2005-03-17 |
JP4478415B2 (ja) | 2010-06-09 |
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