WO2004031745A2 - Controle de qualite de champs de detection dans des jeux ordonnes de micro-echantillons - Google Patents

Controle de qualite de champs de detection dans des jeux ordonnes de micro-echantillons Download PDF

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Publication number
WO2004031745A2
WO2004031745A2 PCT/EP2003/010795 EP0310795W WO2004031745A2 WO 2004031745 A2 WO2004031745 A2 WO 2004031745A2 EP 0310795 W EP0310795 W EP 0310795W WO 2004031745 A2 WO2004031745 A2 WO 2004031745A2
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
microarray
sensor
control
detection
Prior art date
Application number
PCT/EP2003/010795
Other languages
German (de)
English (en)
Other versions
WO2004031745A3 (fr
Inventor
Holger Ottleben
Harald Rau
Robert Schnepf
Dirk Vetter
Original Assignee
Graffinity Pharmaceuticals Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Graffinity Pharmaceuticals Ag filed Critical Graffinity Pharmaceuticals Ag
Priority to AU2003280344A priority Critical patent/AU2003280344A1/en
Publication of WO2004031745A2 publication Critical patent/WO2004031745A2/fr
Publication of WO2004031745A3 publication Critical patent/WO2004031745A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus

Definitions

  • the samples are not immobilized directly on the support, but instead an organic, in this case polymeric, layer is chosen as the binding matrix on which the samples are immobilized.
  • Self-assembling monolayers which are used for masking carrier surfaces are to be mentioned as further organic layers for use as a binding matrix which are described very advantageously in the prior art (see Langmuir 1987, 3, 932-950). These are particularly suitable because the self-assembling Molecules result in a tightly packed film that, for example, avoids the undesired interaction of a target molecule with the mostly inorganic carrier.
  • the entire sensor array-side microarray surface can be blocked between the immobilization of the control ligand and the application of its interaction partner. Blocking may be required to perform the detection.
  • the blocking reagent can be chemical in nature, for example mercaptoethanol with a thiol-sensitive binding matrix. Furthermore, blocking by reagents such as BSA, Tween, Triton or SDS. Such blocking reagents are known to the person skilled in the art.
  • structured microarrays are preferred.
  • the structuring can be carried out, for example, chemically by photostructuring treatment of a binding matrix applied to a planar carrier.
  • the sensor fields are thus defined by the nature or modification of the binding matrix.
  • the sensor fields differ of the sensor field intermediate areas only chemically. However, this distinction is recognizable before the ligands are immobilized and the sensor field position is thus determined before they are applied.
  • An example of such an array is given in WO 00/67028 A1.
  • X is sulfur, for example in the form of the disulfide function (-SS-), the thiol function (-SH) or the sulfide function (-S-).
  • the elements used are characterized by the fact that they either have a high affinity for metals, in particular noble metals (gold, silver etc.), and thus allow the anchor molecules to be immobilized, for example on a gold, silver or platinum surface, or if so is an ionic group, can bind to a metal oxide surface such as Al 2 O 3 . If X has two free valences, both can be connected to the same or different residues - RY.
  • the mobile interaction partner is preferably applied over the entire surface to the surface of the microarray on the sensor field in a suitable solution (e.g. buffer solution).
  • a suitable solution e.g. buffer solution.
  • the concentration of the mobile interaction partner should be chosen so that sufficient interaction with the control ligand is possible.
  • a concentration of the mobile interaction partner of 200nM (avidin tetramer) was sufficient for biotin / avidin.
  • any protective groups that may be present are split off and the product is then split off from the resin with 48/48/4 (v / v / v) trifluoroacetic acid / dichloromethane / triethylsilane, which is subsequently purified by RP-HPLC and analyzed by LC / MS.

Landscapes

  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)

Abstract

L'invention concerne des procédés pour le contrôle de la qualité de champs de détection dans des jeux ordonnés de micro-échantillons.
PCT/EP2003/010795 2002-09-30 2003-09-29 Controle de qualite de champs de detection dans des jeux ordonnes de micro-echantillons WO2004031745A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003280344A AU2003280344A1 (en) 2002-09-30 2003-09-29 Quality control of microarray sensor fields

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10245651.8 2002-09-30
DE2002145651 DE10245651A1 (de) 2002-09-30 2002-09-30 Qualitätskontrolle von Mikroarray Sensorfeldern

Publications (2)

Publication Number Publication Date
WO2004031745A2 true WO2004031745A2 (fr) 2004-04-15
WO2004031745A3 WO2004031745A3 (fr) 2005-01-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/010795 WO2004031745A2 (fr) 2002-09-30 2003-09-29 Controle de qualite de champs de detection dans des jeux ordonnes de micro-echantillons

Country Status (3)

Country Link
AU (1) AU2003280344A1 (fr)
DE (1) DE10245651A1 (fr)
WO (1) WO2004031745A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1811301A1 (fr) * 2004-10-20 2007-07-25 Riken Procede d'observation d'interaction

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10308894A1 (de) * 2003-02-28 2004-09-09 Graffinity Pharmaceuticals Ag Qualitätskontrolle von Bindungsmessungen auf Mikroarrays

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001092883A2 (fr) * 2000-06-02 2001-12-06 Graffinity Pharmaceuticals Ag Surface permettant l'immobilisation de ligants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001092883A2 (fr) * 2000-06-02 2001-12-06 Graffinity Pharmaceuticals Ag Surface permettant l'immobilisation de ligants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FREUNDLIEB S ET AL: "Chemical Microarrays: A Novel Approach to Drug Discovery" NEW DRUGS, AMERICAN MEDICAL ASSOCIATION, CHICAGO, IL, US, Nr. 3, 2001, Seiten 54-60, XP002259718 ISSN: 0545-0985 *
SHEARSTONE J R ET AL: "NONDESTRUCTIVE QUALITY CONTROL FOR MICROARRAY PRODUCTION" BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, Bd. 32, Nr. 5, Mai 2002 (2002-05), Seiten 1051-1052,1054,, XP008011101 ISSN: 0736-6205 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1811301A1 (fr) * 2004-10-20 2007-07-25 Riken Procede d'observation d'interaction
EP1811301A4 (fr) * 2004-10-20 2008-06-18 Riken Procede d'observation d'interaction

Also Published As

Publication number Publication date
WO2004031745A3 (fr) 2005-01-27
AU2003280344A8 (en) 2004-04-23
DE10245651A1 (de) 2004-04-08
AU2003280344A1 (en) 2004-04-23

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