WO2003066842A3 - Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen - Google Patents

Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen Download PDF

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Publication number
WO2003066842A3
WO2003066842A3 PCT/DE2003/000341 DE0300341W WO03066842A3 WO 2003066842 A3 WO2003066842 A3 WO 2003066842A3 DE 0300341 W DE0300341 W DE 0300341W WO 03066842 A3 WO03066842 A3 WO 03066842A3
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WO
WIPO (PCT)
Prior art keywords
plasminogen
nucleic acid
organisms
micro
signal peptide
Prior art date
Application number
PCT/DE2003/000341
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English (en)
French (fr)
Other versions
WO2003066842A2 (de
Inventor
Rudy Susilo
Hans Christian Korting
Hans Guenther Gassen
Martin Hils
Ralf Pasternack
Original Assignee
Trommsdorff Arzneimittel
Rudy Susilo
Hans Christian Korting
Hans Guenther Gassen
Martin Hils
Ralf Pasternack
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trommsdorff Arzneimittel, Rudy Susilo, Hans Christian Korting, Hans Guenther Gassen, Martin Hils, Ralf Pasternack filed Critical Trommsdorff Arzneimittel
Priority to JP2003566193A priority Critical patent/JP2005525798A/ja
Priority to AU2003210137A priority patent/AU2003210137A1/en
Priority to EP20030737248 priority patent/EP1472346A2/de
Priority to MXPA04007585A priority patent/MXPA04007585A/es
Priority to US10/503,910 priority patent/US20050124036A1/en
Priority to DE2003190418 priority patent/DE10390418D2/de
Priority to CA002475277A priority patent/CA2475277A1/en
Publication of WO2003066842A2 publication Critical patent/WO2003066842A2/de
Publication of WO2003066842A3 publication Critical patent/WO2003066842A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Ophthalmology & Optometry (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Dermatology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicinal Preparation (AREA)

Abstract

Beschrieben wird ein Verfahren zur Herstellung von rekombinantem, funktionellem Plasminogen in Mikroorganismen, und ein Verfahren zur Identifizierung von Plasminogenaktivatoren. Die für den funktionellen Teil des Plasminogen kodierende Nukleinsäuresequenz wird mit einem Nukleinsäuremolekül fusioniert, das für mindestens ein Signalpeptid kodiert, wobei das für das Plasminogen kodierende Nukleinsäuremolekül und das für das Signalpeptid kodierende Nukleinsäuremolekül mit Kodons für Schnittstellen von Proteasen verknüpft sind, welche die Abspaltung des Signalpeptids gewährleisten. Das rekombinante Plasminogen oder das entsprechende Plasmin ist geeignet, langsam oder nicht heilende Wunden durch Applikation des Enzyms in einer geeigneten Formulierung zu behandeln.
PCT/DE2003/000341 2002-02-06 2003-02-06 Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen WO2003066842A2 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2003566193A JP2005525798A (ja) 2002-02-06 2003-02-06 微生物内での組み換えタンパク質の生産方法
AU2003210137A AU2003210137A1 (en) 2002-02-06 2003-02-06 Method for producing recombinant proteins in micro-organisms
EP20030737248 EP1472346A2 (de) 2002-02-06 2003-02-06 Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen
MXPA04007585A MXPA04007585A (es) 2002-02-06 2003-02-06 Metodo para la produccion de proteinas recombinantes en microorganismos.
US10/503,910 US20050124036A1 (en) 2002-02-06 2003-02-06 Method for producing recombinant proteins in micro-organisms
DE2003190418 DE10390418D2 (de) 2002-02-06 2003-02-06 Verfahren zur Herstellung von rekombinanten Proteinen in Mikroorganismen
CA002475277A CA2475277A1 (en) 2002-02-06 2003-02-06 Method for producing recombinant proteins in micro-organisms

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP02002716.5 2002-02-06
EP02002716 2002-02-06
US35780902P 2002-02-21 2002-02-21
US60/357,809 2002-02-21

Publications (2)

Publication Number Publication Date
WO2003066842A2 WO2003066842A2 (de) 2003-08-14
WO2003066842A3 true WO2003066842A3 (de) 2004-06-10

Family

ID=36743321

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2003/000341 WO2003066842A2 (de) 2002-02-06 2003-02-06 Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen

Country Status (7)

Country Link
EP (1) EP1472346A2 (de)
JP (1) JP2005525798A (de)
CN (1) CN1768138A (de)
AU (1) AU2003210137A1 (de)
CA (1) CA2475277A1 (de)
MX (1) MXPA04007585A (de)
WO (1) WO2003066842A2 (de)

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CN101563100B (zh) * 2006-08-28 2013-08-07 李季男 用于预防和治疗牙周病、改善牙周创伤愈合以及促进口腔健康的新药物靶标
EP2056865B1 (de) 2006-08-28 2013-12-25 Omnio Healer AB Neues arzneimitteltarget zur verhinderung und behandlung von parodontalerkrankungen, verbesserung der heilung von parodontalwunden und förderung der mundgesundheit
CN102482338B (zh) 2009-07-10 2016-09-28 斯路姆基因公司 纤溶酶原和纤溶酶的变体
EP2494030A4 (de) * 2009-10-30 2013-03-27 Merck Sharp & Dohme Verfahren zur herstellung therapeutischer proteine in pichia pastoris ohne dipeptidyl-aminopeptidase-aktivität
KR20140015289A (ko) 2011-01-05 2014-02-06 쓰롬보제닉스 엔.브이. 플라스미노겐 및 플라스민 변이체
CN103764163A (zh) 2011-08-12 2014-04-30 斯路姆基因公司 纤溶酶原和纤溶酶变体
DK3395354T3 (da) 2015-12-18 2024-06-03 Talengen Int Ltd Plasminogen til anvendelse i behandling af diabetisk nefropati
US11400142B2 (en) 2015-12-18 2022-08-02 Talengen International Limited Treatment of diabetic nerve injury comprising administering plasminogen
CN106890320A (zh) * 2015-12-18 2017-06-27 深圳瑞健生命科学研究院有限公司 一种用于预防或治疗急性及慢性血栓的方法
DK3391902T3 (da) 2015-12-18 2023-11-20 Talengen Int Ltd Plasminogen til anvendelse i behandlingen af diabetisk angiokardiopati
EP3395359B1 (de) * 2015-12-18 2023-11-01 Talengen International Limited Plasminogen zur verhinderung oder behandlung von akuter und chronischer thrombose
US20190151421A1 (en) * 2015-12-18 2019-05-23 Talengen International Limited Method for preventing or treating liver tissue damage and associated diseases
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US10874721B2 (en) 2015-12-18 2020-12-29 Talengen International Limited Method for preventing and treating cervical erosion
CN108463236A (zh) 2015-12-18 2018-08-28 泰伦基国际有限公司 一种预防或治疗放射性和化学性损伤的方法
CN106890319A (zh) * 2015-12-18 2017-06-27 深圳瑞健生命科学研究院有限公司 一种预防或治疗糖尿病性视网膜病变的方法
CA3008691A1 (en) * 2015-12-18 2017-06-22 Talengen International Limited Method for preventing or treating diabetic retinopathy
CN110114079A (zh) 2016-12-15 2019-08-09 泰伦基国际有限公司 一种预防和治疗肥胖症的方法和药物
EP3556384B1 (de) 2016-12-15 2024-04-10 Talengen International Limited Plasminogen zur behandlung von diabetes
WO2018107687A1 (zh) 2016-12-15 2018-06-21 深圳瑞健生命科学研究院有限公司 一种预防和治疗高脂血症的方法
CN108210902A (zh) * 2016-12-15 2018-06-29 深圳瑞健生命科学研究院有限公司 预防和治疗脂肪肝的药物及其用途
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Also Published As

Publication number Publication date
WO2003066842A2 (de) 2003-08-14
AU2003210137A8 (en) 2003-09-02
MXPA04007585A (es) 2005-09-20
CN1768138A (zh) 2006-05-03
AU2003210137A1 (en) 2003-09-02
CA2475277A1 (en) 2003-08-14
EP1472346A2 (de) 2004-11-03
JP2005525798A (ja) 2005-09-02

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