Classifications

• • C—CHEMISTRY; METALLURGY
  • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
  • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
  • C08H99/00—Subject matter not provided for in other groups of this subclass, e.g. flours, kernels
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
  • A23B2/00—Preservation of foods or foodstuffs, in general
  • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
  • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
  • A23B2/729—Organic compounds; Microorganisms; Enzymes
  • A23B2/733—Compounds of undetermined constitution obtained from animals or plants
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F1/00—Treatment of water, waste water, or sewage
  • C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
  • C02F1/683—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water by addition of complex-forming compounds
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
  • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
  • C12H1/02—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material
  • C12H1/04—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material
  • C12H1/0416—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of organic added material
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2101/00—Nature of the contaminant
  • C02F2101/10—Inorganic compounds
  • C02F2101/20—Heavy metals or heavy metal compounds
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2101/00—Nature of the contaminant
  • C02F2101/30—Organic compounds
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2101/00—Nature of the contaminant
  • C02F2101/30—Organic compounds
  • C02F2101/38—Organic compounds containing nitrogen

Definitions

• the present invention is related to complexating systems, intermediates for their production as well as to method for obtaining and using said complexating systems.
• the present invention exhibits particularly advantageous applications in different fields of industry such as food industry, biotechnology or metallurgy, especially when removal of charged macromolecules or metal ions from solutions is sought.
• Complexating agents are widely used in industry to remove undesirable target molecules from solutions for purification, stabilisation, and de-pollution purposes .
• Said complexating agents correspond chemically to chelating molecules able to bind by non-covalent binding to one or more target molecules by non-covalent binding.
• Criteria for the selection of competitive complexating agents are selectivity, specificity, stability and ease of use .
• Tannins are naturally occurring substances derived from phenolic compounds such as gallic acid, which are present for example in plant material such as in gall nuts, tree barks such as oak and hemlock and leaves such as sumac. These plant materials have been used for centuries in their crude form for their ability to tan skins. Tannins are nowadays used in different industrial fields for their complexating properties towards both metals and proteins, for example in the textile industry as mordant and fixative, in galvanoplastics as gelatin precipitant, as clarifying agent in wine manufacture and brewing, in the pharmaceutical industry or in paper industry as sizing agent and mordant for colored papers .
• WO 97/20216 a method is described using silane modified inorganic oxide supports.
• a covalently bound polyphenol may be obtained.
• the synthesis of this product should be performed with dry silica, in an aprotic solvent, results in the release of hydrogen during ' the reaction, and may not lead to covalently bound material .
• An improved version of the immobilisation can be found in patent publication WO 98/00455.
• the tannin is cross-linked with the epoxide groups of a diglycidyl ether, and adsorbed onto silica. Again, this product lacks the covalent bonding and is synthesised at over 100°C for a long period which is an important disadvantage due to the instability of tannin at these temperatures .
• Document WO 00/37584 discloses a method for stabilising monomers, wherein said monomers are placed in contact with an effective amount of at least one radical polymerisation inhibitor or retarder such as catechol or hydroquinone .
• said inhibitor or retarder is immobilised on a solid support, such as silica or a silica-based mineral support, via a coupling agent, for example SiC14.
• Said document concerns also radical polymerisation inhibitors or retarders immobilised on a solid support .
• the present invention aims to provide an immobilised tannin system and a method using said system which do not present the drawbacks of the systems and methods of the state of the art mentioned hereabove .
• the present invention aims to provide a system and a method which allow the complexation of target molecules, preferably charged target molecules of high molecular weight such as polypeptides or proteins, or metal ions, contained in a medium.
• Another aim of the present invention is to provide a system and a method which allow such complexation with a high efficiency.
• Another aim of the present invention is to provide a system and a method, wherein said system is able to form with the target molecules complexes of sufficient stability for further processing.
• the present invention also aims to provide a system and a method which allow the easy removal of target molecules from said medium.
• Another aim of the present invention is to provide a system and a method offering the maximum of guarantees in terms of security, for both the manipulator and the future consumer, as there is a need for a complexating system as a technical aid in food processing.
• the present invention aims to provide a system and a method allowing the possible separate recovery of both the system and the target molecules after extraction from the medium.
• the present invention is related to a complexating system comprising a tannin immobilised on a solid support by means of a bifunctional spacer which is covalently bound from one hand to said solid support by a first function and from the other hand to said tannin by a second function, wherein the tannin is a naturally occurring tannin and exhibits at least six hydroxyl groups .
• the tannin exhibits more hydroxyl groups, preferably more than 8 and advantageously more than 15 hydroxyl groups .
• covalently bound refers to a bond between two atoms, wherein electrons are shared between said atoms .
• Naturally occurring tannins are high molecular weight phenolic compounds occurring in plant material. They have molecular ' weights of over 300 up to 3000, and possess up to 30 and even more hydroxyl groups. They are divided into two categories: hydrolysable tannins and condensed tannins. They have the presence of a large amount of phenolic hydroxyl ' groups in common, leading to a high complexating ability.
• the tannin of the complexating system according to the present invention is selected from the group consisting of hydrolysable tannins and condensed tannins .
• hydrolysable and condensed tannins refer to compounds as disclosed in the standard work " Chemistry of vegetable tannins” by E. Haslam, Ed. Academic Press, London, 1966.
• examples of such tannins which may advantageously be used in the present invention are tannins obtained from plant extracts and commercialised under the trade names Brewtan ® , TANAL, TANEX, FLOCTA , TEXTAN and BIOGRADE .
• said tannin is an hydrolysable tannin comprising a central nucleus such as glucose or quinic acid esterified with a number of compounds selected from the group consisting of gallic acids, ellagic acids, digallic acid, trigallic acid, ellagic acid, cinnamic acid, and derivatives based on these compounds, such as alkylated gallic acid or esterified gallic acid.
• a central nucleus such as glucose or quinic acid esterified with a number of compounds selected from the group consisting of gallic acids, ellagic acids, digallic acid, trigallic acid, ellagic acid, cinnamic acid, and derivatives based on these compounds, such as alkylated gallic acid or esterified gallic acid.
• hydrolysable tannins comprising a glucose as central nucleus are the tannin found in Chinese gall nuts or in Aleppo nuts .
• hydrolysable tannins comprising quinic acid as central nucleus
• Tara tannin is Tara tannin.
• Other hydrolysable tannins extracted from plants are also convenient for the present invention, such as Bengal Kino tannins, Sumach tannins, Vietnamese tannins or Acer tannins or tannins of other plants, said tannins differing from each other in the origin of the central part, in the relative abundance of the composing parts of the product mixture, and in their molecular weight distribution.
• said tannin is a condensed tannin selected from the group consisting of flavanoids, flavanols and stilbene derivatives or a mixture thereof.
• Condensed tannins may be extracted from heartwood or bark of trees, such as Eucalyptus or Acacia tannins, Quebracho tannins or from other plant sources, such as Persimmon or Kaki tannins, often used for the clarification of sake.
• the solid support in the complexating system of the present invention is selected from the group of inorganic oxides.
• said solid support is a silica, a silica prepared in the reaction mixture from its monomer or a silicone polymer.
• inorganic oxides such as alumina, titanium oxides, zirconium oxides, silica aluminates, aluminosilicates, clays, acid-treated clays, or alkaline earth silicates, may also be used as solid support .
• the bifunctional spacer in the complexating system according to the present invention is SiCl .
• the bifunctional spacer has the following general formula II:
• R 1 , R 2 are identical or different and represent an alkyl group with 1-8 carbon atoms, preferably methyl, ethyl or isopropyl, an aryl group, an alkoxy group with 1-8 carbon atoms, preferably methoxy, ethoxy or isopropoxy, or an aryloxy group or an halogen;
• R 3 is a hydrogen, an alkyl group with 1-8 carbon atoms, preferably methyl, ethyl or isopropyl, an aryl group, an alkoxy • group with 1-8 carbon atoms alignment, preferably methoxy, ethoxy or isopropoxy, an aryloxy group or an halogen;
• R 4 is an alkyl chain or an alkylcycloalkyl chain containing 1-10 carbon atoms, or an ether chain containing 2-9 carbon atoms around the oxygen;
• the R!R 2 R3-si group of the bifunctional spacer in the formula II is a trimethoxysilyl, a triethoxysilyl group, a dimethyl-silylhydride or a dimethylsilylchloride .
• the bifunctional spacer is glycidoxy-propyltrimethoxysilane or glycidoxypropyltriethoxysilane, such as those available from Sivento under the trade names Dynasylan GLYMO ® and Dynasylan GLYEO ® .
• the present invention is also related to an intermediate product useful for the preparation of the complexating system and having the following formula IV: SP* - Tan
• Another object of the present invention concerns an intermediate product useful for the preparation of the complexating system and having the following formula V: BB-SP*
• the present invention is also related to a method for obtaining the complexating system of the invention, said method comprising the steps of forming the intermediate products mentioned hereabove and then either immobilising the intermediate product SP* - Tan on the solid support or reacting the intermediate product BB- SP* with the tannin.
• Another object of the present invention concerns a method for removing charged target molecules contained in a medium, preferably an aqueous medium, said method comprising the following steps: - providing the complexating system of the invention and the medium comprising the target molecules;
• aqueous medium a solution comprising water (acidic, neutral, or basic) or other solvents, especially solvents compatible with food applications, such as methanol, ethanol, acetone or the like or mixtures of these solvents.
• the target molecules to be removed from said medium are metals.
• the target molecules are preferably biopolymers .
• the present invention is also related to the use of the complexating system or the intermediate product or the method according to the invention, for applications in food industry, in biotechnology, in pharmaceutical industry, in metallurgy, in electronic industry, in textile industry, in waste water treatment or in paper industry.
• the invention concerns the use of the products or methods of the invention for the clarification and stabilisation of beverages, the treatment of textiles, or the separation and/or purification of proteins .
• Example of the use of the complexating systems according to the invention is the treatment of textile fibers in order to avoid discoloration or staining.
• Another object of the invention concerns the use of the complexating system of the invention as a radical scavenger, an antibacterial or an antioxidant .
• Combinations of tannins with different properties may be used for obtaining the complexating systems in order to improve the performance of the final products i.e. of the complexating systems.
• the naturally occurring tannins described above may be chemically modified with simple molecules such as acids or alcohols in order to enhance or change the properties of the final product, depending on the application.
• esters of tannin with cinnamic acid and similar aromatic acids find application as UV- protectors, antioxidants or radical scavengers.
• UV- protectors for example, esters of tannin with cinnamic acid and similar aromatic acids find application as UV- protectors, antioxidants or radical scavengers.
• the immobilisation of these tannin esters will lead to a new class of UV-absorbers with all the advantages insoluble product offers.
• the spacer is chosen so as to create a distance between the active tannins and the carrier, said distance being sufficient for not negatively altering the properties of the tannin.
• the spacer if containing a stereocenter, may be used as a racemate or in its enantiomeric form.
• the use of inorganic oxides as solid support offers at least two advantages.
• the first advantage is their mechanical stability, useful when the product is recycled or intensively manipulated.
• the second advantage is their relatively high specific surface, which will lead to highly active porous products.
• the inorganic oxide is preferably silica.
• Said silica may be prepared as a hydrogel, a xerogel or another precipitated or crystallised form of silica or regular silica such as MCM-41 or MCM-48 or the like. Other forms may also be envisaged.
• the inorganicoxide should have a minimum particle size, as extremely small particles may lead to slow filtrations.
• the particles may have an average particle size of 1-1000 ⁇ m, depending on the requirements for the specific application or may be clusters of smaller particles with the cluster size within the same range.
• particle size preferably is 4- 20 ⁇ .
• the use of larger particles may be necessary when a quick filtration is a requisite, such as in the removal of metals and proteins by complexation and filtration.
• the specific area of silica preferably varies between 100 and 1500 m 2 /g. The physical characteristics are measured with the state of the art techniques.
• the particle size distribution is measured with laser diffraction techniques, the specific area is measured with N 2 -BET, the pore size distribution is measured with Hg-porosimetry, SEM microscopy is used to view the external form and the homogeneity of the material .
• the chemical behaviour of silica largely depends on the amount of free silanols in the material.
• a silica with a large number of silanol groups is used. This is checked by IR and solid state NMR. The water content of the silica is controlled.
• a pre-treatment of silica may be useful.
• the particle size or particle size distribution may be adjusted by sieving or milling.
• the amount of silanol groups may be increased by an acidic or basic aqueous treatment of the silica, or decreased by a thermal treatment to obtain the desired amount of silanol groups.
• a dehydration step without affecting the silanol groups may also be necessary for good clean reactions.
• inorganic oxides such as alumina, titanium oxides, zirconium oxides, silica aluminates, aluminosilicates, clays, acid-treated clays, alkaline earth silicates, may also be used as carrier material.
• the inorganic oxide may also be functionalised, containing a reactive group for the coupling with tannin. More specifically, the functionalised inorganic oxide is silica that may be silica functionalised for use as a scavenger reagent in combinatorial synthesis, with an alkyl group ending in an epoxide, an isocyanate, a halogenide, an alkylsulphonate, an acid chloride, a sulfonyl chloride or a silylhydride group or a vinyl group.
• the reactive group may be separated from the silica by an alkyl or ether chain composed of 1 to 8 carbons.
• the functionalised silica must be considered to be BB-SP** in formula 1, which is reacted with tannin.
• the inorganic oxide may also be used as an extrudate to facilitate the treatment or the filtration characteristics.
• the spacer molecule may also be reacted with the monomer of the inorganic oxide. More specific, alkoxysilanes, alkoxytitanates, aluminium alkoxides, silicium chlorides, aluminium chlorides and titanium chlorides or mixtures of these monomers, used for the synthesis of the inorganic oxides defined above may be applied. More specific, tetraethoxysilane is most commonly used for the synthesis of silica.
• Cyclic siloxanes, titanates or aluminates such as octamethyltetrasiloxane may also be used as a monomer.
• Oligomers of the inorganic oxides may also be used , e.g. silica colloid suspensions or sols or gels.
• the spacer molecule may also react with silicium-containing polymers, or may also be part of commercially available polymers.
• the polymers may be silicone polymers containing reactive silicium groups that can react with the spacer molecules described above or with SP*-Tan.
• the preparation may either start with the coupling of the solid support, preferably silica, with a spacer molecule, or with the coupling of the tannin with a spacer molecule, or may be a one pot system.
• the amount of tannin used must be at least 1 weight/weight percent, preferably 3 to 50 weight percent with respect to the final material, and in case no polymeric material is used, up to 90 w/w % tannin.
• the amount of the spacer molecule used may vary between approximately 0.1 and 6.0 mole equivalents calculated from the average molecular weight of the tannin.
• the immobilised tannin will be prepared in aqueous medium.
• a first synthetic way comprises the production of an intermediate by the reaction of tannin with spacer.
• This reaction is preferably executed in aqueous medium, in alcohols, ketones or mixtures thereof, at a pH varying from 7 to 12, preferably 8-9.5, and at a temperature of 0-100°C, preferably 20-60°C.
• An inorganic or organic base is used to set the pH of the mixture, preferably a metal hydroxide such as NaOH, NaHC0 3 , or Na 2 C0 3 , ammonia, or triethylamine and the like.
• the reaction is controlled via HPLC-analysis, and may take from 15 minutes to 24 hours depending on temperature and pH.
• the solution of the tannin-spacer obtained by the process may be used as such or purified. It may also be spray dried or freeze dried to a solid for further use.
• Purification of SP*-Tan thus produced may be achieved by membrane separation of low molecular weight compounds, by distillation of volatiles, by extraction or precipitation of the material, by slurrying in organic solvent.
• the SP*-Tan may then be coupled with an inorganic oxide by mixing e.g. silica with tannin-spacer in water, aqueous medium, alcohols or ketones, or mixtures thereof.
• the mixture is subsequently acidified to pH 1-7, preferably to pH 2-5, and reacted at 0-100°C, preferably 20-40°C.
• Acids used include mineral acids such as hydrochloric acid or organic acids such as formic or acetic acid.
• the SP*-Tan may also be combined with monomer of an inorganic oxide, such as tetraethyl orthosilicate, aluminium triethoxide or titanium (IV) isopropoxide .
• SP*-Tan and monomer are mixed in a solvent such as water, aqueous mixtures, a ketone or an alcohol, or mixtures thereof.
• the polymerisation is effected either by acidic or by basic catalysis.
• Acid catalysts can be any protic acid, such as HC1.
• Basic catalysis can be effected by ammonia or by ammonia buffered with ammonium salts or by organic amines such as triethylamine.
• sol gel polymerisation affords to influence the structure of the material obtained by changing the reaction conditions. In this way, modifications in physical and chemical properties of the material can be achieved. Additionally, surfactants may ' be added to influence the physical structure of the final material.
• the SP*-Tan in water may also serve as the monomer.
• the mixture is acidified to pH 1-7, at 0-100°C, preferably 20-40°C, and stirred for 1-24 hours until completion of the polymerisation.
• the R 1R2R3-silicium group reacts into a polymeric backbone on which the tannin is attached.
• Second synthetic approach [0078]
• the spacer is reacted first with the inorganic oxide.
• the condensation can be performed in any solvent, but preferably uses a solvent that is suitable for food grade applications such as water, alcohol, ketones or mixtures thereof.
• the reaction of the inorganic oxide with the spacer in aqueous medium or in polar solvents such as alcohols, ketones or mixtures thereof is carried out at pH 1-7, at 0-100°C, preferably 20-60 °C for 1 to 24 hours.
• the spacer may be added to an acidic or basic suspension of silica, or acid or base is added to a suspension containing silica and spacer.
• the material is collected either by filtration and washings or by spray drying. It may be used as such in the following step, or it may be dried in a vacuum oven at 20-100°C.
• Said inorganic oxide-spacer reaction product is then suspended in aqueous medium or in a polar solvent such as an alcohol or a ketone or a combination of the above. Tannin is subsequently added, and the pH is adjusted to 7-12, preferably to 8-10, ' and the reaction mixture is heated to 20-100°C, preferably to 20-60°C. The reaction mixture is stirred until a satisfying amount of tannin is immobilised.
• a polar solvent such as an alcohol or a ketone or a combination of the above.
• the pH can be ' adjusted to 7-12 using a range of bases, such as ammonia, metal hydroxide, carbonates, organic bases either dissolved in water, or in a pure form.
• bases such as ammonia, metal hydroxide, carbonates, organic bases either dissolved in water, or in a pure form.
• Preferred is sodium hydroxide.
• the spacer molecule is polymerised with the inorganic oxide monomer, and then tannin is coupled onto the formed polymer. In this way, a variant of a functionalised silica gel may be obtained.
• the spacer molecules do not necessarily end with a leaving group, but may end with an unsaturated group, such as an isocyanate or an alkene or conjugated alkene, that can serve as acceptor for a phenolate anion.
• an unsaturated group such as an isocyanate or an alkene or conjugated alkene
• the reaction mixture has to be low in water content, and should be driven in aprotic solvents and without excess of base. All sequences of reacting tannin, spacer and polymeric backbone (carrier) that are described above are applicable to spacers containing any group as defined above.
• the addition of phenolates to unsaturated double bonds may be effected both by acidic and basic catalysis. Acid catalysis may be effected using Lewis acids, mineral acids and sulfonic acids. Basic catalysis may only lead to reaction when activated alkenes are used, e.g. alkenes conjugated with carbonyl derivatives.
• the reaction may be performed under inert nitrogen atmosphere. Prior to isolation, the structure of the immobilised tannin may be modified by adding an inorganic or organic acid or base, to a pH suitable for the specific application. The product is then collected by spray drying or by filtration or centrifugation and may be washed.
• An alternative synthetic pathway is to react carrier with spacer or spacer with tannin in the spray drier. This technique is called reactive spray drying. The reaction can also take place in a rotavapor or in a rotating dryer.
• the water used to mix the reactives evaporates, and the substances react with each other.
• the inlet temperature of the spray dryer may vary from just above 100°C up to 250°C, with an outlet temperature of 60-200°C.
• Filtration of the final material should be relatively fast, as this parameter is essential for the future use of the product. Indeed, the materials prepared show a good filtration speed.
• Purification of the product may be achieved by reslurrying in aqueous solution or in polar solvents such as alcohols, ketones or mixtures thereof, or by distillation of volatiles. This purification may remove residual spacer, tannin, salts or solvents.
• the product is finally dried in a vacuum oven at 20-100°C if required.
• Further analyses include inorganic ash, KF, residual solvent, residual tannin measurement.
• the immobilised tannin prepared as described may be used as such, as a gel, as a spray-dried product or in formulations or as composite materials with other compounds depending on the properties required.
• the complexating system of the invention may be used for the known applications of tannins. Most applications are based on the capacity of tannin to complexate proteins or metals.
• the first group of applications for immobilised tannin is the uptake and removal of proteins.
• proteins and yeast residues are removed to obtain a clarified beer without using large quantities of filter aid. This is an advantage over the classical beer clarifications with tannin itself.
• immobilised tannins may be applied in a column or in another continuous mode or may be recovered. A major advantage is the absence of tannin residues in the beverage.
• Immobilised tannin may also be used for treatment of other beverages, such as wine, sake, port, fruit juices, lemonades.
• Immobilised tannin may also be used in the purification of enzymes or proteins, as there is selectivity depending on the composition and the molecular weight of the protein.
• the complex formation with proteins may lead to applications in ' the chromatographic field.
• Proteins may also be immobilised on immobilised tannin.
• the protein uptake is checked by treating a standard protein solution with immobilised tannin, followed by measurement of the residual amount of protein in the supernatant .
• Immobilised tannin can also be applied to complexate and remove metal ions from any solution, such as beverages, waste water, water containing precious metals, water containing heavy metals, chemical reaction mixtures, radioactive waste water, organic solvents or organic mixtures containing metals.
• the insoluble products and/or the particulate spacer-tannin may also be applied to burns, to improve crust formation and to avoid infections. These antibiotic properties of tannin may also be used to avoid unwanted bacterial growth onto solid material exposed to air.
• Application of SP*-Tan as such onto a target material is also possible. In this approach, a solution of SP*-Tan is contacted with a support that has to be treated with tannin. The mixture is then acidified, and the SP*-Tan polymerises around the material to form a polymeric coating.
• This material may be textile or textile fibres, which benefit from a tannin treatment to enhance the colour fastness, wash fastness or anti-staining properties of the textile.
• the system has the advantage over the classical tannin treatments that there is no leaching of the tannin when the textile is washed, thus the original activity remains fully present, also after repeated washing.
• Metallic surfaces may also be treated this way to avoid oxidation.
• a pre-treatment such as etching may be required to activate the surface.
• SP*-Tan may also be co plexated with dyestuffs.
• the resulting complex composed of SP*-Tan and the dye may then be used as an ink component for application onto textile, paper or any material to be dyed or printed.
• polymerisation of the adsorbed SP*-Tan-dye complex may be effected by acidification or by heating the material. In this way, the dye is immobilised both by complexation and by inclusion into the polymer matrix, leading to improved wash fastness and water fastness.
• SP*-Tan is a very strong antioxidant and an effective radical scavenger.
• Silica and titanium oxide are used as fillers in a variety of products such as paints, textiles and polymers.
• the antioxidant and radical scavenging properties of tannin may be applied by immobilising SP*-Tan onto these fillers, such as silica, titanium oxide, zirconium oxide or other inorganic oxides. These immobilised tannins may be used in paints to avoid colour shifting, and thus to increase their stability; in textiles as protection against ageing or against chlorine contact in swimming pools . Description of preferred embodiments of the invention
• a reactor is charged with 107 g Brewtan ® and 500 ml water.
• the solution is treated with NaOH 30% to a pH of 9.50.
• a total of 78.5 g of base solution is added.
• the solution is then heated to 60°C.
• 107 g glycidoxypropyltrimethoxysilane is added over a period of 25 minutes.
• the mixture is stirred at 60°C for 2 hours and slowly cooled to ambient temperature.
• the solution is spray dried yielding a nice, brown powder.
• a reactor is charged with 50.00 g. of silica Sipernat ® 50 and with 350 mL water.
• the pH of the resulting suspension is increased to 9.15 by the addition of 10 mL NaHC0 saturated and 29 mL Na 2 C0 3 saturated.
• the suspension is warmed to 26°C and 30.00 mL glycidoxy- propyltrimethoxysilane is added.
• the pH is further increased by the addition of 8,0 mL saturated Na 2 C0 3 solution.
• the suspension is reacted for 3 hours at 26°C, filtered, washed with 250 mL of water and 250 mL of MeOH and dried in a vacuum dryer at 60°C over night.
• Brewtan ® 14 g is dissolved in 250 ml demineralised water, and treated with NaOH 1 M to a pH of 9.5. Subsequently, 40 g of Daraclar ® 7500 based silica- spacer is then added, and the suspension is heated to 60°C for 3 hours. After cooling, the product is collected on a pressure filter and washed with 10 times 100 ml demi water and dried. This yields 39.7 g of slightly brown product. Brewtan is a Chinese gallotannin with between 12 and 30 hydroxyl groups .
• B ⁇ chi Mini Spray Dryer B-191 with an inlet temperature of 215°C and with an outlet temperature of around 140°C.
• a reactor is charged with 30. g Brewtan ® and
• Example 9 TEOS + SP*-Tan polymerisation [0117] SP*-tannin as prepared in Example 1 (Scale:
• Example 11 One pot system [0119] 30 g of Si0 2 for flash chromatography (Merck
• a solution of 20.0 g ⁇ Quebracho tannin was treated with NaOH 30% to a pH of 9.2. and 20.00 g glycidoxypropyltrimethoxysilane was added to this solution at 43°C over a period of 15 minutes. The solution was then stirred for 4 hours, and subsequently added onto a suspension of silica Daraclar 7500 in 240 ml water and 20 ml acetic acid 100 %. The suspension is then stirred over night, filtered and washed with 500 ml of water. After drying to constant weight, 10.65 g immobilised, condensed tannin is obtained. Quebracho tannin has > 4 hydroxyl groups, and a number of constituents possess > 10 hydroxyl groups) .
• albumine test can be drawn as : Immotan/albumine complex
• the immobilised tannin is weighed to obtain an estimated 10 mg tannin equivalent based on Thermogravimetric analysis. This polymer is swollen in buffer solution at pH 3.5 for 1 hour. 10 ml of 0.2 wt . % Bovine Serum Albumine (BSA) is then added, and the suspension is stirred for 1 hour at room temperature. The suspension is then centrifugated, and the supernatant is then quantified after combination with Coomassie blue.
• This test also known as cold alcohol test, is based on the precipitation of proteins with EtOH.
• beer is treated with tannin or immobilised tannin and filtered. 100 g filtrate is then mixed with 8.0 ml ethanol (96% v/v) and cooled for 60 min at -5°C. Measurement of the turbidity after 60 minutes affords a figure directly related to the protein content of the treated beer.
• a blank without any treatment and the classical in solutio treatment with 2 g/hL Brewtan were carried out as reference points.
• Immotan is weighed to obtain approximately 10 mg tannin equivalent and combined with 5 ml of buffer and a stirbar. The suspension is stirred for 1 hour. The suspension is then combined with 5 ml 50 ppm Fe(III) solution, stirred for exactly 30 minutes, and filtered over a 0.45 ⁇ m filter.
• the adsorption capacity is the number of mg Fe adsorbed by 1 gram of tannin immobilised.
• a first main advantage is the fact that the high amount of phenolic hydroxyl groups of the tannins used indeed leads to much higher complexation activity.
• Another advantage is that said complexating systems may be easily obtained and lead to a removal of target molecules with a high yield. It is particularly important that the preparations described are fully compatible with the requirements for products used in food processing. [0130] Moreover, they are sufficiently stable to resist to industrial processing, even when they chelate target molecules, but they may also be easily dissociated, when desired, for recovering separately the target molecules from one part and the complexating systems from the other part .
• complexating systems i . e . the tannin, the solid support and the spacer according to both the applications and the target molecules .
• Said complexating systems may be used in pure form but in particular cases, it may also be appropriate to use a mixture of the complexating systems of the invention.

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