WO2003026683A1

WO2003026683A1 - Health caring product for enhancing immunity and delaying aging

Info

Publication number: WO2003026683A1
Application number: PCT/CN2002/000616
Authority: WO
Inventors: Xiaoping Zhu; Yaohui Zeng
Original assignee: Xiaoping Zhu; Yaohui Zeng
Priority date: 2001-09-05
Filing date: 2002-09-04
Publication date: 2003-04-03
Also published as: CN1336235A; CN1121237C

Abstract

The present invention relates to a oral health caring product for enhancing immunity and delaying aging. It is composed of Ganoderma, Cordyceps and extract of Lumbricus by ratio of 1-4:0.5-2.5:0.5-3. Many tests had demonstrated that it has powerful resistance to oxidation, can enhance body's resistibility to disease and can delay aging. It is an useful diet for those who are in the condition of immunodeficiency. It is also suitable to be administrated to tumour patients after surgery or in combined with radiotherapy and chemotherapy. It can prevent postoperative recurrence and transference of tumour.

Description

Health products capable of regulating immune function and delaying aging

Technical field

The invention relates to a health care product capable of regulating immune function and delaying aging, in particular, it is a pure natural oral health care product which is made of Chinese herbal medicine with the same origin of medicine and food.

Background technique

At present, various diseases caused by immunodeficiency and immune disorders are increasing day by day. In particular, cancer has always been the number one enemy that seriously threatens human health. It is the greatest harm faced by more than 5 billion people worldwide. According to incomplete statistics, today, due to cancer, The number of deaths is 1/4 of the natural death of humans, that is, about 6 million people die from cancer every year in the world, of which more than 1.5 million are in China. In recent years, the incidence of cancer in China has continued to rise, and the incidence of Younger, until now, except for the treatment of cancer by surgery, radiotherapy, and chemotherapy, there have been no special breakthroughs. Although there are nearly one hundred tumor drug companies, there are seven major categories of drugs and more than one hundred varieties. Among them, there are more than ten kinds of antibiotics, 7-8 kinds of phytochemicals, more than ten kinds of deepening agents, nearly ten kinds of anti-metabolites, and more than ten kinds of enzymes, hormones and other classes. The effect is not ideal, and it has toxic and side effects.

In order to reduce and prevent the occurrence of various diseases (including cancer), health foods can regulate the body's immune function, so as to improve the body's resistance, enhance people's health, and reduce the incidence of diseases.

Summary of the Invention

The task of the present invention is to provide a health care product that can regulate immune function and delay aging, thereby improving people's resistance to disease, reducing the occurrence of diseases, enhancing people's health, and also supporting the coordination of postoperative and radiochemical processes for tumor patients. Use to prevent recurrence and metastasis after surgery. To achieve the above tasks, the following technical solutions are used:

A health-care product capable of regulating immune function and delaying aging, which is characterized in that it is an oral health-care product made by pulverizing and mixing the following weight ratio raw materials at a temperature of -15 ° C:

Ganoderma Lucidum 1-4 parts Cordyceps sinensis 0. 5-2. 5 parts Ground Dragon Extract 0. 5-3 parts.

The Ganoderma lucidum and Cordyceps sinensis powder is pulverized to a temperature of less than 800 mesh at a temperature of -15 ° C; the ground dragon extract is prepared by the following method, first the ground dragon is washed and added 1. 5-4. 5 times normal saline, autolysis at 25-50 ° C, centrifugation to remove residue, collect the supernatant for the first ultrafiltration, M is 70,000 to 100,000 columns, and the collected ultrafiltrate is subjected to a second time Ultrafiltration, M is 1.5-25,000 columns, the retentate was chromatographed on a G-75 column, washed with physiological saline, and the active peaks were collected and freeze-dried to obtain the Dilong extract; the said Health products are capsule preparations.

The health product of the present invention uses Cordyceps sinensis powder, Ganoderma lucidum powder, and Dilong powder extract, and is thoroughly mixed to prepare an oral health product. The formula is based on:

The raw materials of the health products of the present invention are all precious Chinese herbal medicines with the same origin of medicine and food, and have been summarized through a large number of experimental studies. Among them:

Ganoderma:

"Compendium of Materia Medica" contains "Yinxinqi, Buzhong, wisdom enhancement, do not forget", "long-term food", light-weight, long-lasting fairy "and so on. Ganoderma is the fruiting body of Basidiomycete polymycete Ganoderma lucidum, often Tissue separation method was used to isolate and cultivate Ganoderma lucidum fruit body.

The chemical composition of Ganoderma lucidum is very complicated. So far, more than 150 compounds have been isolated from Ganoderma lucidum. Many experiments have shown that Ganoderma lucidum acid, Ganoderma lucidum polysaccharide, Ganoderma adenosine and organic germanium are the main active ingredients of Ganoderma. Antitumor activity. Ganoderma has the functions of analgesia, sedation, inhibition of histamine release, detoxification, liver protection, and toxin killing of tumor cells. Ganoderma adenosine derivative It has a strong effect of lowering blood viscosity, inhibiting the accumulation of small plates in the body, improving blood oxygen supply capacity and accelerating blood circulation.

Experiments have shown that Ganoderma lucidum can increase the activity of superoxide dismutase (SOD), have antithrombotic formation and improve the ability of red blood cells to deform, can promote the proliferation of lymphocytes, and enhance the phagocytosis and lethality of macrophages, cells and T cells.

Cordyceps sinensis:

The Compendium of Materia Medica contains "Sweetness and peace of mind, entry into the lungs and kidneys of the two meridians, tonifying the lungs and kidneys, invigorating the essence, relieving cough, phlegm, treating cough, and treating dysentery." Artificial Cordyceps sinensis is a silk body produced by fermentation of Cordyceps fungi isolated from fresh samples of Cordyceps sinensis. The experimental analysis showed that the components of cultured Cordyceps sinensis were equivalent to those of natural Cordyceps sinensis. The effects are basically the same, providing a basis for the clinical of the two.

The composition of Cordyceps sinensis contains mainly polysaccharide, cordycepin, cordycepin, more than 20 kinds of acid and 17 kinds of inorganic elements.

A large number of experiments have proved that: natural Cordyceps sinensis and its artificial cultured mycelium preparation can accelerate the rate of spleen nucleic acid and protein renewal, promote DNA synthesis, and help accelerate the division and proliferation of spleen cells, thereby enhancing immunity, giving mice 4-8g / kg , Can significantly promote the proliferation of mouse bone marrow granule-monoline progenitor cells and enhance bone marrow hematopoietic function.

It was observed that the activation of cordyceps sinensis and artificial mycelium extracts on mouse peritoneal macrophages may be a non-specific immune enhancer through phagocytosis experiments in mice, cross-matching tests in vitro, and acid phosphatase activity measurement.

By observing the immunoregulatory effects of Cordyceps sinensis polysaccharides and the tonic effect on the body, it has been initially proved that it is an effective ingredient of Cordyceps sinensis's tonic and nourishing effects. Cordyceps polysaccharide has obvious activation effect on phagocytic function of reticuloendothelial system and peritoneal macrophages, and has certain effects on lymphocyte transformation. enhancement.

Cordyceps sinensis and Cordyceps mycelium 3g / kg can increase the content of superoxide dismutase (SOD) in liver tissue of mice. In vitro experiments have similar results and can inhibit the formation of lipid shield peroxides. Cordyceps mycelium can inhibit the activity of monoamine oxidase in the brain of rats, and its effect on immune function suggests that Cordyceps sinensis and Cordyceps mycelium have anti-aging effects.

Earthworm:

It mainly contains hypoxanthine, fatty acids, succinic acid, cholesterol, amino acids, nucleic acid derivatives, dilodin, fibrinolytic enzyme, dilotoxin, and a large number of trace elements.

Through autolysis, the isolated small molecule is extracted. The results of animal experiments show that it can significantly increase the number of thymic and spleen nucleated cells in mice, increase the phagocytic function of mouse macrophages, and make white blood cells in peripheral blood. The increase in number reveals that this component has the effect of improving the body's immunity.

When mice were inoculated with S ₁₈ . And H ₂₂ and S ₂₉₄ , EMT-6 and other tumor cells, ^ B or gastric perfusion with Dilong extract, showing good antitumor effect. The tumor weight in the administration group was significantly different from that in the control group (P <0.01).

The experiment also proved that Dilong extract has a good effect of activating plasminogen and directly dissolving fibrin in the blood, reducing blood viscosity.

After the three components are compatible with each other, they have complementary advantages, and may form an attractant similar to a biological response modifier (BRM). It mainly mobilizes the BRM process in the body by enhancing the immune function of the body, improves M cell activity, enhances phagocytosis, and increases White blood cells can improve the symptoms of patients with tumors and improve the patient's tolerance to radiotherapy and chemotherapy. Because this product has strong antioxidant capacity, this product has the function of improving the body's resistance to diseases. The health product of the present invention is used as a test substance to carry out tests of regulating immune function and delaying aging effect, and the results are as follows: The test is based on the "functional evaluation procedure and detection method of health food",

1. Regulate immune function:

Kunming female secondary mice weighing 18-22 g were randomly divided into four groups according to body weight. The test amount was a human body recommended product of 1.8 g / day and 60 kg body weight. It was divided into three doses: low, medium and high. The drug was administered once a day, and the control group was perfused with water, and the results were measured for four consecutive weeks. Results:

(1) Effect on body weight of normal mice: See Table 1 Table 1: Effect on body weight of normal mice (X soil SD) Group dose Amount of animals Number of weight before experiment P value Weight of after experiment P value

(g / kgbw) (only) (g) (g) Control group 0 12 21.9 ± 1.4 1 32.7 ± 3.1 1 Low dose 0.15 12 21.7 ± 2.2 0.8021 33.9 ± 2.1 0.2803 Medium dose 0.30 12 22.1 ± 1.5 0.7634 34.7 ± 3.7 0.1700 High dose 0.60 12 21.8 ± 1.0 0.7946 33.5 ± 2.7 0.4654 As can be seen from the table above, there were no significant differences between the dose groups before and after 4 weeks of oral administration of different doses of test substance to the control group (P> 0.05). ).

(2) Effects on delayed-type allergies in normal mice: See Table 2 Table ² : Effects on delayed-type allergies (DTH) in normal mice (X soil SD) Group dose (g / kgbw) Number of animals (only ) Difference in stopping swelling (mm) P value

Control group 0 12 0.53 ± 0.15 1

Low dose 0.15 12 0.69 ± 0.15 * 0.0024

Medium dose 0.30 12 0.83 ± 0.24 ** 0.0034

High dose 0.60 12 0.77 ± 0.14 ** 0.0015

* Significantly different from the control group (P <0.05) ** Compared with the control group, there is a very significant difference (P <0.01).

As can be seen from the table above, after oral administration of different doses of test substance to mice for four weeks, compared with the control group, the toe swelling of the low and high dose groups increased by 30% (P <0.05) and 52% (P <0. 01), and% (P <0. 01)

(3) Effect on serum hemolysin of normal mice: see Table 3

Table 3: Effect on serum hemolysin of normal mice (X ± SD)

* Compared with the control group (P <0.05). As can be seen from the table above, four weeks after administration of different doses of test substance to oral mice, compared with the control group, the HC50 in the middle and high dose groups increased to 1.7% and 2.1 ° / ο (P <0. 05 ).

It can be seen that, after oral administration to mice of different doses of the test substance for four weeks, compared with the control group, toe swelling in the low-dose group (0.15 g / kgbw) was increased by 30% (P <0.05), and other indicators There were no significant differences (P> 0.05), the toe swelling degree in the middle dose group (0.3 g / kgbw) increased by 57% (P <0.01), and the half hemolysis value increased by 1.7% (P <0 05). In the high-dose group (0.6 g / kgbw), toe swelling increased by 45% (P <0.01), and half hemolysis value increased by 2.1% (P <0.05).

2. Anti-aging effect:

The test animals and doses were the same as before, and the indicators of each group of mice were measured after 58 days of continuous drug administration. test results:

(1) Effect on the content of lipid peroxide in mouse serum:

(a) Serum lipid peroxide content is determined as shown in the table below. Table 4: Determination of serum lipid peroxide content

After mixing, dissolve at 95 ° C for 7 minutes (open the lid and boil) for 40 minutes. After taking out, centrifuge at 3500-20007min for 10 minutes, and adjust the color of distilled water to 532 legs.

(b) Effect on serum lipid content in mice, see Table 5

Table 5: Effect on serum lipid content in mice (X ± SD)

* Significantly different from the control group.

The above table shows that after 58 days of oral administration of different doses of test substance to aged mice, compared with the blank control group, the serum lipid peroxide content in the middle dose group was reduced by 13% (P <0.05).

(2) Measurement of glutathione peroxidase activity in mouse whole blood is performed according to the following table, see Table 6

(a) Measurement of glutathione peroxidase activity in mouse whole blood

Table 6: Measurement of glutathione peroxidase activity in mouse whole blood Test reagent Sample tube (ml) Non-enzymatic tube (ml) Empty white tube (ml)

G S H 0.4 0.4 1

Blood sample dilution 0.4 1 1

Double distilled water ml 1 0.4 I H ₂ 0 ₂ (ml) 37 ° C Pre-temperature 0. 2 0. 2 1

Metaphosphoric acid precipitation solution 4. 0 4. 0 1

3000r / min centrifugation min

Centrifugal supernatant (ml) 2. 0 2. 0

Double distilled water (ml) 1 1 0. 4

Partial acid precipitation solution 1 1 1. 6

Na ₂ HP0 ₄ (ml) 2. 5 2. 5 2. 5

DTNB coloring solution (ml) 0.5 5 0. 5 0. 5 color reaction 1 read D value at 420nm wavelength for 1 minute, the measurement results are shown in Table 7.

(b) Results of effects on blood glutathionase activity in aged mice

Table 7: Effect on glutathionase activity in whole blood of aged mice (

* Compared with control group (P <0. 05)

It can be seen that, compared with the blank control group (0. 15g / day / kg. Bw), the various doses of the test substance were orally administered to the aged mice for 58 days without significant difference in each index (P> 0. 05), the serum lipid shield peroxide content in the middle dose group was increased by 13% (P <0. 001), the number of glutathione peroxidase activity units in whole blood; and the high dose group was in whole blood glutathione Peroxidase activity units increased by 11 ° / ο (P <0.05).

3. For S ₁₈ . Tumor inhibition experiment: see Table 8

Table ⁸ : For S ₁₈ . Results of Tumor Suppression Experiment (X + SD) The other U dose (g / kg. Bw) in the group was the number of animals (only tumor weight (g) inhibition rate (%) P value control group 1 10 4. 46 ± 2. 54 0

Administration group 1. 05 / kg. Bw 10 2. 35 ± 1. 91 * 43. 78 0. 025 As a result of the test, the test substance was S ₁₈ . The tumor inhibition rate was 48.59% (P <0.05).

To sum up, different doses of the health product of the present invention were orally administered to aged mice, compared with the control group after 4 weeks: the toe swelling degree of the low, medium and high dose groups was increased by 30% (P <0.05) , 521 (P <0. 01) and 45% (P <0. 01). The median and high dose groups had significant differences in serum hemolysis values compared with the control group, which increased by 1.7% and 2.1%, respectively (P <0, 05). Inventing health products, compared with the blank control group at 58 days: the medium-dose group (0. 30g / kg. Bw), the serum lipid shield peroxide content was increased by 13% (P <0.001). In the high-dose group (0.60 g / kg. Bw), the number of glutathione peroxidase activity units in whole blood increased by 11% (P <0.05), compared with S ₁₈ . Sarcoma has an inhibitory effect, and its tumor inhibition rate is 48.59% (P <0.05), and there is no significant difference in body weight before and after dose experiment in each group (P> 0.05).

According to the "Functional Evaluation Procedures and Testing Methods of Health Food", the health product of the present invention has the functions of regulating immune function and delaying aging, and it can also inoculate sarcoma S ₁₈ . Has a suppressing effect.

As a result of adopting the above scheme, the technology of the present invention has the following advantages and effects:

a) The product of the present invention uses raw materials of the same origin as medicine and food. It is a health product that can regulate immunity and delay aging. It is suitable for patients with immune dysfunction, and it is also suitable for tumor patients after surgery and during chemotherapy and radiotherapy. Used together to prevent recurrence and metastasis after surgery.

b) The formula is scientific and reasonable. After the three components are compatible with each other, the advantages are complementary. It can form a kind of attractant similar to biological response modifier (BRM). By enhancing the body's immune function, it can mobilize the BRM process in the body, improve NK cell activity, and enhance phagocytic cells. Phagocytosis function, increase white blood cells to improve the symptoms of tumor patients, improve patients' tolerance to radiotherapy and chemotherapy, and docking Seed sarcoma S ₁₈ . Has a suppressing effect.

detailed description

Example 1:

First, the ground dragon was washed, and 1.5 times normal saline was added at 50 ° C to perform autolysis. After centrifugation to remove the residue, the collected supernatant was subjected to the first ultrafiltration. M was 70,000 columns. The filtrate was subjected to secondary ultrafiltration, with M of 25,000 columns, and the retentate was chromatographed on a G-75 column, washed with physiological saline, and the active peaks were collected, and freeze-dried to make ground dragon extract for future use. The Ganoderma lucidum and Cordyceps sinensis were pulverized to 800 mesh at a temperature of -15 ° C, and made into powder for use. Finally, the above-prepared Dilong extract 0.5 kg, Ganoderma lucidum 4 kg and Cordyceps sinensis 1.5 kg, at -15 ° C The granules are mixed into granules at the temperature, and the health product of the present invention is obtained.

Example 2:

First, the earthworm was washed, 3.0 times normal saline was added for autolysis at a temperature of 40 ° C, and the residue was collected by centrifugation, and the collected supernatant was subjected to the first ultrafiltration. The M was 80,000 columns. The filtrate was subjected to a second ultrafiltration, and the M was 20, 000 columns. The retentate was chromatographed on a G-75 column, washed with physiological saline, and the active peaks were collected, and the ground dragon extract was prepared by water-drying for use. 8kg, 在-It is mixed with granules at 15 ° C and filled with gum ft, which is the health product of the present invention.

Example 3:

First, the earthworm was washed, and 4.5 times normal saline was added at 25 ° C for autolysis. After centrifugation to remove the residue, the collected supernatant was subjected to the first ultrafiltration. The M was 100,000 columns. The filtrate was subjected to a second ultrafiltration, with M of 15 thousand columns, and the retentate was chromatographed on a G-75 column using normal saline. Rinse, collect the active peaks, freeze-dry to make ground dragon extract for future use, and then crush the ganoderma lucidum and cordyceps -15 ° C to 1250 mesh to make powder for future use. Finally, take the above-prepared ground dragon extract 3 Okg, Ganoderma lucidum 1. Okg and Cordyceps 0.5 kg, mixed and granulated at -15 ° C and loaded into the thigh, which is the health product of the present invention.

Claims

Rights request

1. A health care product for regulating immune function and delaying aging, which is characterized in that it is an oral health care product made by pulverizing and mixing raw materials with the following weight ratio at a temperature of -15 ° C:

2. The health care product according to claim 1, wherein the ganoderma lucidum and cordyceps are between -15. Powder crushed to below 800 mesh at C temperature.

3. The health product according to claim 1, characterized in that said Dilong extract is prepared by the following method, and the Dilong is washed by adding 1. 5-4. 5 times physiological saline at 25 Auto-dissolve at -50 ° C, remove the residue by centrifugation, collect the supernatant for the first ultrafiltration, M is 70,000-100,000 columns, and the collected ultrafiltrate is subjected to secondary ultrafiltration, M is 1. 5-2. 5 million columns. The retentate was chromatographed on a G-75 column, washed with physiological saline, and the active peaks were collected. The freeze-dried solution was the Dilong extract.

4. The health product according to claim 1, wherein the health product is a capsule preparation.