CN100365011C - Process for producing asparagus saponin - Google Patents
Process for producing asparagus saponin Download PDFInfo
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- CN100365011C CN100365011C CNB200510048265XA CN200510048265A CN100365011C CN 100365011 C CN100365011 C CN 100365011C CN B200510048265X A CNB200510048265X A CN B200510048265XA CN 200510048265 A CN200510048265 A CN 200510048265A CN 100365011 C CN100365011 C CN 100365011C
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- asparagus
- saponin
- asparagus saponin
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Abstract
The present invention discloses a method for producing asparagus saponin, which comprises the steps of pulverization of raw materials, the addition of 2 to 10 times of ethanol, heating from 50 to 100DEG. C, and extraction for 1 to 10 hours. A crude product of fine powder is obtained by filtration, condensation, addition of precipitating agents, and centrifugal cooling and drying, and the crude product is added with macroporous adsorptive resin for separating and purifying. Accordingly, the refined product of the asparagus saponin is obtained. The present invention has wide sources of raw materials and simple and convenient production process. The asparagus saponin has the efficiency of preventing cancers, resisting cancers, reducing blood lipid, reducing cholesterol, etc. The present invention can be used for preparing anti-cancer health care medicine and health care food.
Description
Technical field
The present invention relates to the extracted form natural plant production technique.Relate to a kind of production method of asparagus saponin specifically.
Background technology
Asparagus saponin has great medical treatment, health care is worth.Modern medicine study proves that one of main effect link of existing antitumor drug is to disturb or block the synthetic of intracellular nucleic acid and protein and other.Bibliographical information is arranged, and asparagus saponin has the effect that promotes the fracture of enzyme mediated dna, so one of its antitumor action link may be to suppress to realize by the biomacromolecule synthetic selectivity such as DNA, RNA of performance asparagus saponin to cancer cells.Asparagus saponin contains nine kinds of steroid matter saponin materials, and multiple cancer is all had better curative effect.U.S.'s " cancer Newsweek " is also to this detailed textual criticism.It is to introduce asparagus saponin like this in " beat cancer " (Chinese well-known doctor talks one of all kinds of diseases and ailments book series) book that academician of the Chinese Academy of Sciences, molecular weight tumor are learned the director Professor Zhang of National Key Laboratory: asparagus saponin all has prevention and therapeutic efficiency to various cancers, especially bladder cancer, lung cancer, skin carcinoma is had special efficacy.
From the seventies, along with developing rapidly of isolation technique and structural research means, more and more cause people's attention for the chemical research of asparagus saponin, especially bigger to polarity, sugar chain is longer, and the research of the more complicated saponin of structure has had breakthrough progress.Up to the present, China has been the important country of mass production diosgenin (diosgenin), Tigogenin (tigogenin) and extra large Ke sapogenin (hecogenjn).
In recent years, along with the development of separating and purifying technology and structural analysis measuring method, the chemical research of saponin has been obtained great breakthrough, and its structure and biochemical function have also obtained continuous announcement.Asparagus saponin can be anticancer, become the common recognition of world the world of medicine in today, is used to protective foods or the health care medicine producing a new generation's anti-cancer, control cancer with being widely used.
At present, China also is in the starting stage to the extraction of asparagus saponin, the research of purifying, in this respect, still finds no the document that closes open asparagus saponin production technology.
Therefore, this area presses for the production method of a complete asparagus saponin technology.
Summary of the invention
The production method that the purpose of this invention is to provide a kind of asparagus saponin makes its asparagus saponin that obtains be used to give protection against cancer, control the protective foods or the health care medicine of cancer.
The present inventor finds that through going deep into extensive studies the asparagus raw material is added certain density alcohol extraction, separation in production technique, purifying can directly make asparagus saponin.Finished the present invention on this basis.
At this on the one hand, the invention provides the production method of asparagus saponin, its method steps is:
(a) choose the asparagus raw material, put into container and pulverize, extraordinarily go into the ethanol of 50%～95% concentration then by the 2-10 of raw material volume, be heated to 50 ℃-100 ℃, extract and formed mixing solutions in 1-10 hour;
(b) poly-to going on foot (a) forms mixing solutions and filters, and concentrates, adds precipitation agent, centrifugal cooling drying obtains asparagus saponin powder crude product;
(c) with step (b) asparagus saponin powder crude product, any of adding macroporous adsorbent resin such as AB-8, X-5, NAK-9 carries out separation and purification, thereby obtains asparagus saponin powder highly finished product.
Among the present invention, what be used as raw materials for production is meant fresh asparagus, dry product asparagus or asparagus tankage.The asparagus raw material is tackled it and is washed, dries before use.
In the present invention, term " mixing solutions " refers to form through extraction after asparagus production technique raw material through pulverizing and the ethanol mixing.
In the present invention, asparagus raw material and alcoholic acid mixed volume are very important for the method for invention.General ethanol is by 2-10 times of asparagus raw material volume; It preferably is 3-8 times.
In the present invention, described precipitation agent is to be selected from any of ether, sherwood oil, or their mixture.
In the present invention, the control concentration of ethanol is to implement another crux of the present invention, is the 50%-95% except that adding concentration, and preferably concentration is 50%-90% (by weight percentage).
In the present invention, employed macroporous adsorbent resin is to be selected from AB-8, the material of easily separated purifying such as X-5, NAK-9.
In the methods of the invention, adopt the asparagus raw material, do the extraction agent extraction, add precipitation agent and produce precipitation by asparagus saponin through filtering with ethanol, centrifugal, add precipitation agent, cooling drying obtains crude product; Crude product is promptly made the asparagus saponin highly finished product through the macroporous adsorbent resin separation and purification.Have the starting material wide material sources, processing method is easy.Can be used for producing cancer therapy drug and fall three high class healthcare products.
Each index detects and sees the following form in the asparagus saponin of the present invention:
| The project indicator | Detected result |
| Proterties | Buff powder |
| Asparagus saponin content | 81.1％ |
| Moisture content | 4.1％ |
| Ash | 3.6％ |
| Arsenic | 1.2mg/kg |
| Heavy metal | 3mg/kg |
| Total plate count | 200/gram |
| Flavacin | 0.1ppb |
| Intestinal bacteria | 0 |
| Salmonellas | 0 |
| Mould+yeast | 40/gram |
Another characteristics of the present invention:
1, the present invention is that to select natural edible-plant for use be raw material, produces asparagus saponin and is used to prevent and treat cancer, and is nontoxic to human body.
2, can improve the immunizing power of tumour patient, suppress to dwindle local tumor.
3, be used to strengthen tumour patient physique before the art, ensureing performs the operation carries out smoothly, promotes wound healing, reduces postoperative infection.
4, strengthen the sensitization of chemicotherapy, remove that chemicotherapy causes feel sick, toxic side effecties such as vomiting apocleisis, sleep are poor, alopecia, pain, the rising red white corpuscle.
5, prevent the transfer and the recurrence of chemicotherapy and postoperative tumour.
6, blood fat reducing, the prevention cardiovascular and cerebrovascular diseases.
Asparagus saponin composition of the present invention: every 100g contains total saponin of 80g; Proterties: light yellow powder, the gas mildly bitter flavor has water absorbability, is not easy crystallization, and is soluble in water and pure, is insoluble in organic solvent.
Usage and dosage: add by being grown up 120mg/ days.
Embodiment
EXAMPLE l
Its production stage is:
1, the first-selected fresh asparagus raw material is cleaned dried, and takes by weighing 250g and pulverizes standby;
2, the above-mentioned asparagus of pulverizing of weighing is put into container, add the ethanol of the mass concentration 55% of 3 times of volumes, be heated to 50 ℃, extracted 1 hour, carry out subcooling;
3, above-mentioned refrigerative compound filters, solution after the filtration adds 50% petroleum ether precipitation agent 10ml, the lipid-soluble substance precipitation is descended, carry out the filtrate after the sedimentation centrifugal, make water soluble saccharide separating substances in its supernatant liquor, its residue throw out is carried out lyophilize, and the crude product that obtains light yellow powder is standby;
4, the crude product of above-mentioned yellow powder being added macroporous adsorbent resin X-5 adsorbs to take off and washes;
5, the ethanolic soln that the above-mentioned compounding substances that takes off after washing is added mass concentration 30% takes off and washes, and makes to take off the rate of washing and reach 95.3%, separates after purified again, and cooling drying promptly obtains the buff powder highly finished product.
In above-mentioned, macroporous adsorbent resin is select that Tianjin resin processing plant of Nankai University produces for use adsorbs to take off as X-5, AB-8, three kinds of resins of NAK-9 and wash.
In above-mentioned, related separating and filtering equipment can select for use the medical separating device of selling in the market to get final product.
Embodiment 2
1, taking by weighing the 250g asparagus pulverizes standby;
2, the above-mentioned asparagus of pulverizing of weighing is put into container, add 95% ethanol of the mass concentration of 7 times of volumes, be heated to 95 ℃, extracted 5 hours, carry out subcooling;
3, above-mentioned cooling mixing material is filtered, sherwood oil (precipitation agent) 10ml of the solution adding 50% after the filtration descends the lipid-soluble substance precipitation, carry out the filtrate after the sedimentation centrifugal, make water soluble saccharide separating substances in its supernatant liquor, its residue throw out is carried out lyophilize, and the crude product that obtains yellow powder is standby;
4, above-mentioned yellow powder crude product being added macroporous adsorbent resin AB-8 adsorbs to take off and washes;
5, the ethanolic soln that the above-mentioned compounding substances that takes off after washing is added mass concentration 90% takes off and washes, and makes to take off the rate of washing and reach 95.3%, separates after purified, cooling drying again, promptly obtains the asparagus saponin highly finished product of buff powder.
Claims (4)
1. the production method of an asparagus saponin, its method steps is:
(a) choose the asparagus raw material, put in the container and pulverize, extraordinarily go into the ethanol of 50%-95% concentration, be heated to 50 ℃-100 ℃, extract and formed mixing solutions in 1-10 hour by the 2-10 of raw material volume;
(b) mixing solutions that step (a) is formed filters, concentrates, adds precipitation agent, centrifugal, cooling drying obtains asparagus saponin powder crude product;
(c) will go on foot poly-(b) asparagus saponin powder crude product, add macroporous adsorbent resin, wherein said macroporous adsorbent resin select AB-8, X-5, NAK-9 for use any carry out separation and purification, thereby obtain asparagus saponin powder highly finished product.
2. by the production method of the described asparagus saponin of claim 1, it is characterized in that described amount of alcohol added 3-8 times by asparagus raw material volume.
3. by the production method of the described asparagus saponin of claim 1, it is characterized in that described concentration of ethanol is 50%-90%.
4. by the production method of the described asparagus saponin of claim 1, it is characterized in that described precipitation agent is the mixture that is selected from any or they of ether, sherwood oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510048265XA CN100365011C (en) | 2005-12-31 | 2005-12-31 | Process for producing asparagus saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510048265XA CN100365011C (en) | 2005-12-31 | 2005-12-31 | Process for producing asparagus saponin |
Publications (2)
| Publication Number | Publication Date |
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| CN1793165A CN1793165A (en) | 2006-06-28 |
| CN100365011C true CN100365011C (en) | 2008-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB200510048265XA Expired - Fee Related CN100365011C (en) | 2005-12-31 | 2005-12-31 | Process for producing asparagus saponin |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559157B (en) * | 2009-05-18 | 2012-08-22 | 华东师范大学 | Method for fractional extraction of asparagus saponin and asparagus amylose using asparagus slag |
| CN103897012B (en) * | 2014-04-15 | 2015-11-18 | 山西大学 | A kind of method of separation and Extraction officinalis saponin(e in therefrom medicine officinalis |
| TWI617312B (en) * | 2016-11-14 | 2018-03-11 | 國立虎尾科技大學 | Extract of asparagus officinales, the extraction method and the use thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546506A (en) * | 2003-12-04 | 2004-11-17 | 国家海洋局第一海洋研究所 | Method for preparing asparagus rutin |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546506A (en) * | 2003-12-04 | 2004-11-17 | 国家海洋局第一海洋研究所 | Method for preparing asparagus rutin |
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| CN1793165A (en) | 2006-06-28 |
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Effective date of registration: 20190805 Address after: 066004 No. 12 Donghai Road, Qinhuangdao Economic and Technological Development Zone, Hebei Province Patentee after: QINHUANGDAO CHANGSHENG NUTRITION AND HEALTH TECHNOLOGY CO.,LTD. Address before: 066600 Military Housing Management Department, Chengguan Second Street, Changli County, Qinhuangdao City, Hebei Province Patentee before: Huang Yunxiang |
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Effective date of registration: 20210107 Address after: 066318 No. 12 Donghai Road, Qinhuangdao Economic and Technological Development Zone, Hebei Province Patentee after: Huang Yunxiang Address before: 066004 No.12, Donghaidao, Qinhuangdao Economic and Technological Development Zone, Hebei Province Patentee before: QINHUANGDAO CHANGSHENG NUTRITION AND HEALTH TECHNOLOGY Co.,Ltd. |
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