WO2002090554A1 - Vecteur de clonage - Google Patents

Vecteur de clonage Download PDF

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Publication number
WO2002090554A1
WO2002090554A1 PCT/JP2002/004463 JP0204463W WO02090554A1 WO 2002090554 A1 WO2002090554 A1 WO 2002090554A1 JP 0204463 W JP0204463 W JP 0204463W WO 02090554 A1 WO02090554 A1 WO 02090554A1
Authority
WO
WIPO (PCT)
Prior art keywords
gene
host
cloning
sequence
vector according
Prior art date
Application number
PCT/JP2002/004463
Other languages
English (en)
Japanese (ja)
Inventor
Naoto Nemoto
Yoichiro Ito
Original Assignee
Gencom Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gencom Corporation filed Critical Gencom Corporation
Priority to JP2002587614A priority Critical patent/JP4300031B2/ja
Publication of WO2002090554A1 publication Critical patent/WO2002090554A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

Definitions

  • DNA cloning involves inserting a DNA fragment into a vector, introducing it into E. coli, and forming an E. coli colony on a plate to obtain a colony having the target fragment.
  • a method of confirming the introduction of the vector of the inserted DNA sequence (insert) by the inactivation of the ⁇ -galactosidase ⁇ GF ⁇ gene has been reported (blue-white screening or green).
  • -mte screening Yanisch-Perron et al. Gene 33 103-119 (1985); Ito et al. Gene 245 59-63 (2000)).
  • the 64th amino acid is Leu
  • the 65th amino acid is Thr
  • the 9′9th amino acid is Thr.

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un vecteur de clonage, où la présence/l'absence de transfert d'une insertion de séquence ADN peut être déterminée de manière efficace. Selon l'invention, un vecteur de clonage est doté, dans le sens allant de l'extrémité 5' vers l'extrémité 3', d'un site de clonage comportant au moins une enzyme de restriction désignée, une séquence d'attachement du ribosome et un gène pour sélectionner un hôte.
PCT/JP2002/004463 2001-05-09 2002-05-08 Vecteur de clonage WO2002090554A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2002587614A JP4300031B2 (ja) 2001-05-09 2002-05-08 クローニングベクター

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001138214 2001-05-09
JP2001-138214 2001-05-09

Publications (1)

Publication Number Publication Date
WO2002090554A1 true WO2002090554A1 (fr) 2002-11-14

Family

ID=18985187

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/004463 WO2002090554A1 (fr) 2001-05-09 2002-05-08 Vecteur de clonage

Country Status (2)

Country Link
JP (1) JP4300031B2 (fr)
WO (1) WO2002090554A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018012570A1 (fr) * 2016-07-13 2018-01-18 国立大学法人鳥取大学 Procédé de fabrication de virus vaccinal exprimant un gène étranger
JP2020014392A (ja) * 2018-07-23 2020-01-30 国立大学法人山口大学 原核生物用発現ベクター
JP2020018287A (ja) * 2018-07-23 2020-02-06 国立大学法人山口大学 スクリーニング用ベクター

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0213381A (ja) * 1988-06-30 1990-01-17 Wakunaga Pharmaceut Co Ltd 複数発現ベクターおよびこれを用いたタンパク質の製造法
JPH08256776A (ja) * 1995-03-24 1996-10-08 Morinaga Milk Ind Co Ltd 高効率発現ベクター
JP2001204474A (ja) * 2000-01-27 2001-07-31 Gencom Co 緑色蛍光タンパク質を用いたクローニングベクター

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0213381A (ja) * 1988-06-30 1990-01-17 Wakunaga Pharmaceut Co Ltd 複数発現ベクターおよびこれを用いたタンパク質の製造法
JPH08256776A (ja) * 1995-03-24 1996-10-08 Morinaga Milk Ind Co Ltd 高効率発現ベクター
JP2001204474A (ja) * 2000-01-27 2001-07-31 Gencom Co 緑色蛍光タンパク質を用いたクローニングベクター

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ITO Y. ET AL.: "A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 264, no. 2, 1999, pages 556 - 560, XP002954198 *
ITO Y. ET AL.: "A T-extended vector using a green fluorescent protein as an indicator", GENE, vol. 245, no. 1, March 2000 (2000-03-01), pages 59 - 63, XP004202961 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018012570A1 (fr) * 2016-07-13 2018-01-18 国立大学法人鳥取大学 Procédé de fabrication de virus vaccinal exprimant un gène étranger
JPWO2018012570A1 (ja) * 2016-07-13 2019-02-28 国立大学法人鳥取大学 外来遺伝子発現ワクシニアウイルスの製造方法
JP2020014392A (ja) * 2018-07-23 2020-01-30 国立大学法人山口大学 原核生物用発現ベクター
JP2020018287A (ja) * 2018-07-23 2020-02-06 国立大学法人山口大学 スクリーニング用ベクター
JP7108300B2 (ja) 2018-07-23 2022-07-28 国立大学法人山口大学 スクリーニング用ベクター
JP7174990B2 (ja) 2018-07-23 2022-11-18 国立大学法人山口大学 原核生物用発現ベクター

Also Published As

Publication number Publication date
JP4300031B2 (ja) 2009-07-22
JPWO2002090554A1 (ja) 2004-11-04

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