WO2000065904A1 - A process for producing hybrid rice seeds by use of seed colors - Google Patents

A process for producing hybrid rice seeds by use of seed colors Download PDF

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Publication number
WO2000065904A1
WO2000065904A1 PCT/CN2000/000076 CN0000076W WO0065904A1 WO 2000065904 A1 WO2000065904 A1 WO 2000065904A1 CN 0000076 W CN0000076 W CN 0000076W WO 0065904 A1 WO0065904 A1 WO 0065904A1
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Prior art keywords
seed
seeds
color
trait
line
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PCT/CN2000/000076
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French (fr)
Chinese (zh)
Inventor
Xianping Zeng
Qinxiu Li
Yuanwen Yue
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Crop Institute, Sichuan Academy Of Agricultural Sciences
Sichuan Superior Crops Utilization Co.
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Application filed by Crop Institute, Sichuan Academy Of Agricultural Sciences, Sichuan Superior Crops Utilization Co. filed Critical Crop Institute, Sichuan Academy Of Agricultural Sciences
Priority to AU36540/00A priority Critical patent/AU3654000A/en
Publication of WO2000065904A1 publication Critical patent/WO2000065904A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022—Genic fertility modification, e.g. apomixis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

Definitions

  • the invention belongs to the technical field of rice heterosis utilization, and particularly relates to the selection and breeding of a golden-shell sterile line or a restorer line and a method for producing the hybrid.
  • the purpose of the present invention is to provide a method for making hybrid rice seed production simple and easy; and to use existing agricultural tools for sowing, harvesting, and effectively separating hybrid rice seeds from their parents (restoration lines) to produce national seed Standard commercial hybrid seeds.
  • a method for producing hybrid rice seeds by using seed color-marking traits transferring the seed color-marking traits into an existing sterile line or a restorer line, and culturing seed color-marking trait sterile lines or restorer lines at different maturity stages; Seeds with similar color sowing dates for sterile lines and restorer lines without markers, or sterile lines with restorer lines for seedless marks and restorers with color-marked traits, are mixed and sown at a ratio of 5: 1—6: 1.
  • Use the photoelectric separator of the polished rice processor to sort according to the color of the seeds, and separate the seeds with marked traits and those without marked traits. The seeds are sorted to obtain hybrid rice seeds.
  • the seed is marked with a golden shell.
  • the seed color-marker sterility line was Jin GA.
  • the seed color-marking trait restoration line was Jin 422.
  • Hybrid seeding is seeding with a seeder or direct seeding in the field.
  • the seeds are harvested in the field with a harvester or mixed by hand in the field.
  • the seed sorting is to sort the seeds collected in the production field with a photoelectric separator of a refined rice processor to obtain hybrid rice seeds that meet national seed standards.
  • the invention mainly includes two aspects of breeding of golden-shell sterile lines or restorer lines and hybrid rice seed production.
  • Seed production technology The golden-shell sterile line and the grass-colored shell restorer line or the grass-colored-shell sterile line and the golden-shell restorer line with similar sowing dates are formulated.
  • the specific measures are as follows: 1 Seeding: Sterile and restorer seeds Mixed sowing according to the ratio of 5: 1—6: 1, both seeder can be used for seeding or artificial field broadcasting; 2Harvest: You can use the harvester with mixed harvesting in the field, or you can manually harvest in the field according to the seed color; 3 Sorting: The photoelectric separator of the existing polished rice processing machine is used to sort the seeds harvested from the mixed farming field.
  • the photoelectric separation machine (ESM) of the polished rice processing machine uses the color of the seeds as the sorting standard, it is easy to sort the golden shell.
  • the seeds are separated from the grass-colored shell seeds.
  • the test result of the applicant of the present invention is that the sorting rate is 99%, which is higher than the national seed standard. Best Mode of the Invention
  • golden-shell sterile line is Jin GA
  • golden-shell restoring line is Golden 422.
  • the mixed seeds are sorted with a photoelectric separator of a polished rice processor to obtain hybrid rice seeds.
  • the present invention can use the existing agricultural machinery to realize the seed mechanization of the field, sowing, harvesting, labor saving, and reducing production costs.
  • Stable and high yield Firstly, because the present invention adopts the parental seed production of the same maturity period, it is not necessary to adjust the flowering period, thereby greatly reducing the chance of yield reduction due to weather and other factors, which do not meet the flowering period. Random distribution is uniform, pollen is distributed reasonably, and high yield is easy to obtain.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A process for producing hybrid rice (Oryza sativa) seeds by use of seed colors comprises the following steps: introducing the mark character of the seed color into existent male-sterile lines or restorer lines which have different seed maturation period; cultivating them into seeds which can express the mark character of the seed color; mixedly sowing seeds of male-sterile line and restorer line in a ratio 5-6:1, either having the marking character and both having the same sowing period; harvesting seeds of mixedly planted parent plants; selecting hybrid rice seeds according to the seed color by photoelectric separator. The process for producing hybrid rice seeds is simple, and the production of hybrid rice seeds is stable and high.

Description

利用种子颜色生产杂交水稻种子的方法  Method for producing hybrid rice seed by using seed color
技术领域 Technical field
本发明属于水稻杂种优势利用技术领域， 具体涉及一种金色壳不育 系或恢复系的选育及其杂交种的生产方法。  The invention belongs to the technical field of rice heterosis utilization, and particularly relates to the selection and breeding of a golden-shell sterile line or a restorer line and a method for producing the hybrid.
背景技术 Background technique
杂交水稻在中国能得以大规模的生产利用， 主要是靠劳动密集型的 杂交稻种子生产技术。但这种技术存在很多问题， 无法适应机械化制种， 随着社会经济的发展， 农村劳动力的转移， 种子工程产业化的进程加快， 实现机械化制种技术是降低生产成本和种子工程产业化的必然趋势。  The large-scale production and utilization of hybrid rice in China is mainly based on labor-intensive hybrid rice seed production technology. However, this technology has many problems and cannot adapt to mechanized seed production. With the development of society and economy, the transfer of rural labor forces, the process of industrialization of seed engineering is accelerating. The realization of mechanized seed production technology is inevitable to reduce production costs and industrialization of seed engineering. trend.
现有杂交稻种子生产的具体技术缺陷是不育系种子和恢复系种子颜 色均为草色 (淡黄色)， 二者无法区分。 因此， 在种子生产时无法实现机械 化混播、 混收； 无法将杂交种和亲本 (恢复系)分离出来。 发明内容  The specific technical defect of the existing hybrid rice seed production is that the color of the sterile line seed and the restorer line seed are both grass (light yellow), and the two cannot be distinguished. Therefore, mechanized mixed sowing and mixing cannot be achieved during seed production; hybrids and parents (restorative lines) cannot be separated. Summary of the Invention
本发明的目的是提供一项能使杂交稻种子生产简便易行； 并能使用 现有农业机具进行播种、收获和有效地将杂交稻种子与亲本 (恢复系)分离 出来， 生产出符合国家种子标准的商品化杂交种子。  The purpose of the present invention is to provide a method for making hybrid rice seed production simple and easy; and to use existing agricultural tools for sowing, harvesting, and effectively separating hybrid rice seeds from their parents (restoration lines) to produce national seed Standard commercial hybrid seeds.
本发明的内容是这样实现的：  The content of the present invention is realized as follows:
一种利用种子颜色标记性状生产杂交稻种子的方法，将种子颜色标记 性状转育到现有不育系或者恢复系之中， 育成不同熟期的种子颜色标记 性状不育系或者恢复系； 将播抽期相近的种子颜色标记性状不育系与无 标记性状恢复系或者无标记性状不育系与种子颜色标记性状恢复系种子 按 5 ： 1— 6 ： 1的比例混合播种， 收获的种子， 用精米加工机的光电 分离机根据种子颜色迸行分选， 将具有标记性状的种子与没有标记性状 的种子分选而得到杂交稻种子。 A method for producing hybrid rice seeds by using seed color-marking traits, transferring the seed color-marking traits into an existing sterile line or a restorer line, and culturing seed color-marking trait sterile lines or restorer lines at different maturity stages; Seeds with similar color sowing dates for sterile lines and restorer lines without markers, or sterile lines with restorer lines for seedless marks and restorers with color-marked traits, are mixed and sown at a ratio of 5: 1—6: 1. Use the photoelectric separator of the polished rice processor to sort according to the color of the seeds, and separate the seeds with marked traits and those without marked traits. The seeds are sorted to obtain hybrid rice seeds.
种子颜色标记性状为金色壳。  The seed is marked with a golden shell.
种子颜色标记性状不育系为金 GA。  The seed color-marker sterility line was Jin GA.
种子颜色标记性状恢复系为金 422 。  The seed color-marking trait restoration line was Jin 422.
种子混合播种为用播种机播种或者人工田间直播。  Hybrid seeding is seeding with a seeder or direct seeding in the field.
种子收获为用收割机具田间混合收割或者采用人工在田间按种子颜 色单独收割。  The seeds are harvested in the field with a harvester or mixed by hand in the field.
种子分选为将制种田中混收的种子用精米加工机的光电分离机进行 分选， 获得符合国家种子标准的杂交稻种子。  The seed sorting is to sort the seeds collected in the production field with a photoelectric separator of a refined rice processor to obtain hybrid rice seeds that meet national seed standards.
下面进一步叙述本发明的内容：  The content of the present invention is further described below:
本发明主要包括金色壳不育系或恢复系的选育和杂交稻制种两方 面。  The invention mainly includes two aspects of breeding of golden-shell sterile lines or restorer lines and hybrid rice seed production.
1 、 金色壳不育系或恢复系的选育： 水稻谷壳颜色一般分为草色 (淡 黄色：)、 金色 (深黄色：)、 紫色、 黑色。 而现有生产上所用的不育系、 恢复 系和 F ÷子均为草色。 因此将金色壳性状基因输入现有不育系或恢复系 之中， 育成不同熟期的金色壳不育系或恢复系。 现已育成金色壳的不育 系金 GA、 金色壳恢复系金 422等。  1. Breeding of sterility or restorer lines with golden hulls: The color of rice husks is generally divided into grass (light yellow :), golden (dark yellow :), purple and black. The sterile lines, restorer lines and F ÷ seeds used in the current production are grassy. Therefore, the golden-shell trait genes were introduced into the existing sterile lines or restorer lines, and golden-shell sterility or restorer lines of different maturity stages were bred. The golden sterility line Jin GA and golden hull recovery line Jin 422 have been bred.
2 、 制种技术： 将播抽期相近的金色壳不育系与草色壳恢复系或草 色壳不育系与金色壳恢复系进行配制， 具体措施如下： ①播种： 不育系 与恢复系种子按 5 ： 1— 6 ： 1的比例进行混合播种， 既可采用播种机 播种， 也可人工田间直播； ②收获： 可使用收割机具田间混合收割， 也 可采用人工在田间按种子颜色单独收割； ③分选： 采用现有精米加工机 的光电分离机将制种田混合收获的种子进行分选， 因精米加工机的光电 分离机 (ESM)是以籽粒颜色为分选标准，很容易将金色壳种子和草色壳种 子分离开来， 本发明申请人试验结果为分选率 99%, 高于国家种子标准。 本发明的最佳实施方式 2. Seed production technology: The golden-shell sterile line and the grass-colored shell restorer line or the grass-colored-shell sterile line and the golden-shell restorer line with similar sowing dates are formulated. The specific measures are as follows: ① Seeding: Sterile and restorer seeds Mixed sowing according to the ratio of 5: 1—6: 1, both seeder can be used for seeding or artificial field broadcasting; ②Harvest: You can use the harvester with mixed harvesting in the field, or you can manually harvest in the field according to the seed color; ③ Sorting: The photoelectric separator of the existing polished rice processing machine is used to sort the seeds harvested from the mixed farming field. Because the photoelectric separation machine (ESM) of the polished rice processing machine uses the color of the seeds as the sorting standard, it is easy to sort the golden shell. The seeds are separated from the grass-colored shell seeds. The test result of the applicant of the present invention is that the sorting rate is 99%, which is higher than the national seed standard. Best Mode of the Invention
本发明的具体实施方法如下：  The specific implementation method of the present invention is as follows:
1 、 将金色壳性状基因转入不同熟期的不育系或恢复系中， 获得 稳定的、 可利用的金色壳不育系或恢复系。 该金色壳不育系为金 GA ， 该金色壳恢复系为金 422。  1. Transfer the golden shell trait genes into sterile or restorer lines of different maturity stages to obtain stable and available golden shell sterile or restorer lines. The golden-shell sterile line is Jin GA, and the golden-shell restoring line is Golden 422.
2 、 将金色壳不育系与草色壳恢复系或者草色壳不育系与金色壳恢 复系按 5 ： 1—6 ： 1比例进行混合播种， 可采用播种机播种， 也可人 工田间直播。 收获： 可使用收割机具田间混合收割， 也可采用人工在田 间按种子颜色单独收割。 其它田间管理措施 (如施肥、 浇水、 防虫、 防病 等)与一般大田水稻生产相同。  2. Mixed seeding of the golden-shell sterile line and the grass-colored shell restorer line or the grass-colored sterile line and the golden-shell restorer line is carried out at a ratio of 5: 1-6: 1, which can be planted by a seeder, or can be broadcast directly in the field. Harvesting: Combine harvesters can be used in the field to harvest, or they can be harvested individually in the field by seed color. Other field management measures (such as fertilization, watering, pest control, disease prevention, etc.) are the same as those for general field rice production.
3 、 将混收种子用精米加工机的光电分离机进行分选， 而得到杂交 水稻种子。  3. The mixed seeds are sorted with a photoelectric separator of a polished rice processor to obtain hybrid rice seeds.
工业应用性 Industrial applicability
本发明的优点和积极效果如下：  The advantages and positive effects of the present invention are as follows:
1 、 简单易行： 本发明可采用现有农机具实现制种技术的田间机械 化播种、 收获、 节约劳动力、 降低生产成本。  1. Simple and easy: The present invention can use the existing agricultural machinery to realize the seed mechanization of the field, sowing, harvesting, labor saving, and reducing production costs.
2 、 稳产高产： 首先是由于本发明采用同熟期的亲本制种， 不必进 行花期调节， 因而大大地降低因气候等因素造成花期不遇而减产的机 率； 其二是因恢复系在制种田中随机分布均匀， 花粉散布合理， 易于 获得高产。 2. Stable and high yield: Firstly, because the present invention adopts the parental seed production of the same maturity period, it is not necessary to adjust the flowering period, thereby greatly reducing the chance of yield reduction due to weather and other factors, which do not meet the flowering period. Random distribution is uniform, pollen is distributed reasonably, and high yield is easy to obtain.

Claims

权 利 要 求 Rights request
1 、 一种利用种子颜色标记性状生产杂交稻种子的方法， 其特征在 于： 将种子颜色标记性状转育到现有不育系或者恢复系之中， 育成不同 熟期的种子颜色标记性状不育系或者恢复系； 将播抽期相近的种子颜色 标记性状不育系与无标记性状恢复系或者无标记性状不育系与种子颜色 标记性状恢复系种子按 5 ： 1—6 ： 1的比例混合播种， 收获的种子， 用精米加工机的光电分离机根据种子颜色进行分选， 将具有标记性状的 种子与没有标记性状的种子分选而得到杂交稻种子。 1. A method for producing hybrid rice seeds by using seed color-marking traits, characterized in that: the seed color-marking traits are transferred to an existing sterile line or a restorer line, and seed color-marking traits of different maturity stages are sterilized Line or restorer line; mix seed-marked trait sterile lines with similar sowing dates with unmarked trait restorer lines or unmarked trait sterile lines with seed-colored trait restorer lines at a ratio of 5: 1-6: 1 The seeds sowed and harvested are sorted according to the color of the seeds using a photoelectric separator of a polished rice processor, and the seeds having marked traits and the seeds without marked traits are sorted to obtain hybrid rice seeds.
2、根据权利要求 1所述的方法， 其特征在于： 种子颜色标记性状为 金色壳。  2. The method according to claim 1, characterized in that: the seed color marking trait is a golden shell.
3、根据权利要求 1所述的方法， 其特征在于： 种子颜色标记性状不 育系为金 GA。  3. The method according to claim 1, characterized in that: the seed color-marking trait sterile line is golden GA.
4、根据权利要求 1所述的方法， 其特征在于： 种子颜色标记性状恢 复系为金 422。  The method according to claim 1, characterized in that: the seed color marker trait restoration line is Jin 422.
5、根据权利要求 1所述的方法， 其特征在于： 种子混合播种为用播 种机播种或者人工田间直播。  5. The method according to claim 1, characterized in that: the mixed seeding of seed is seeding with a seeder or manual field broadcasting.
6、根据权利要求 1所述的方法， 其特征在于： 种子收获为用收割机 具田间混合收割或者采用人工在田间按种子颜色单独收割。  6. The method according to claim 1, characterized in that: the seed is harvested by using a harvester with mixed harvesting in the field or by manual harvesting according to the seed color in the field.
7、 根据权利要求 1所述的方法， 其特征在于： 种子分选为将制种田 中混收的种子用精米加工机的光电分离机进行分选， 获得符合国家种 子标准的杂交稻种子。  7. The method according to claim 1, characterized in that: the seed sorting is to sort the seeds mixed in the production field with a photoelectric separator of a refined rice processor to obtain hybrid rice seeds that meet national seed standards.
PCT/CN2000/000076 1999-04-29 2000-04-05 A process for producing hybrid rice seeds by use of seed colors WO2000065904A1 (en)

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KR20220170784A (en) * 2021-06-23 2022-12-30 정구훈 Rice cultivation method using seedlings composed of amylose and amylopectin in a target weight ratio

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CN1118232C (en) * 1999-04-29 2003-08-20 四川省农业科学院作物研究所 Method for producing hybrid rice seeds by using seed colors
CA2436203A1 (en) * 2001-02-09 2002-08-22 Monsanto Technology Llc Identification of seeds or plants using phenotyic markers
CN101836580B (en) * 2009-03-19 2012-09-05 中国水稻研究所 Method for simplifying hybrid rice seed production process and improving production efficiency
CN103548630B (en) * 2013-10-18 2016-05-11 杨勋毅 A kind of method that breeding of hybrid rice entire mechanization is produced
CN105706911A (en) * 2016-02-19 2016-06-29 四川省农业科学院生物技术核技术研究所 Simple seed production method for three-line hybrid rice based on sterile line flower fragrance A
CN107173216A (en) * 2017-06-12 2017-09-19 甘肃农业大学 Seed selection and its application in Mixed plant that corn difference pericarp color CMS tri- is

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CN1031008A (en) * 1987-05-27 1989-02-15 围环物产公司 Producing F1 hybrid soybean and recovery is the substantially uniform seed group's of plant improvement method
CN1229576A (en) * 1999-04-29 1999-09-29 四川省农业科学院作物研究所 Method for producing hybrid rice seeds by using seed colors

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US3842538A (en) * 1972-03-13 1974-10-22 Gabonatermesztesi Kutato Intez Method of production of hybrid seed-grain
CN88100355A (en) * 1987-01-06 1988-09-14 围环物产公司 The improved route that hybrid rice produces
CN1031008A (en) * 1987-05-27 1989-02-15 围环物产公司 Producing F1 hybrid soybean and recovery is the substantially uniform seed group's of plant improvement method
CN1229576A (en) * 1999-04-29 1999-09-29 四川省农业科学院作物研究所 Method for producing hybrid rice seeds by using seed colors

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Publication number Priority date Publication date Assignee Title
KR20220170784A (en) * 2021-06-23 2022-12-30 정구훈 Rice cultivation method using seedlings composed of amylose and amylopectin in a target weight ratio
KR102613424B1 (en) 2021-06-23 2023-12-12 정구훈 Rice cultivation method using seedlings composed of amylose and amylopectin in a target weight ratio

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AU3654000A (en) 2000-11-17
CN1229576A (en) 1999-09-29

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