WO2000053744A3 - Selection finale d'une evolution dirigee - Google Patents

Selection finale d'une evolution dirigee Download PDF

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Publication number
WO2000053744A3
WO2000053744A3 PCT/US2000/006497 US0006497W WO0053744A3 WO 2000053744 A3 WO2000053744 A3 WO 2000053744A3 US 0006497 W US0006497 W US 0006497W WO 0053744 A3 WO0053744 A3 WO 0053744A3
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WO
WIPO (PCT)
Prior art keywords
methods
molecules
obtaining
stochastic
provides methods
Prior art date
Application number
PCT/US2000/006497
Other languages
English (en)
Other versions
WO2000053744A2 (fr
WO2000053744A9 (fr
Inventor
Jay M Short
Gerhard Johann Frey
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/267,118 external-priority patent/US6238884B1/en
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Priority to AU38793/00A priority Critical patent/AU3879300A/en
Priority to JP2000603365A priority patent/JP2002537836A/ja
Priority to CA002361927A priority patent/CA2361927A1/fr
Priority to IL14516500A priority patent/IL145165A0/xx
Priority to EP00917887A priority patent/EP1161529A2/fr
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of WO2000053744A2 publication Critical patent/WO2000053744A2/fr
Publication of WO2000053744A3 publication Critical patent/WO2000053744A3/fr
Publication of WO2000053744A9 publication Critical patent/WO2000053744A9/fr
Priority to US10/151,469 priority patent/US20030219752A1/en
Priority to AU2005203719A priority patent/AU2005203719A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Cette invention concerne des techniques permettant d'obtenir des polynucléotides et des polynucléotides codés en utilisant des techniques non stochastiques d'évolution dirigées (DirectEvolution™). La possibilité de récupérer des polynucléotides de pleine longueur issus d'une bibliothèque de molécules d'une lignée générées par mutagenèse présente un avantage particulier dans les techniques fondées sur une sélection finale. Ces techniques comprennent des mutagenèses à saturation de site non stochastiques de polynucléotides (Gene Site Saturation Mutagenesis™) et un réassemblage de polynucléotides non stochastique (GeneReassembly™). Cette invention concerne des techniques permettant d'obtenir des enzymes aux propriétés physiques et/ou biologiques optimisées. Par l'utilisation de ces techniques de l'invention, on peut faire évoluer des vaccins génétiques, des enzymes, de petites molécules et d'autres molécules qui conviennent vers les propriétés souhaitées. On peut, par exemple, obtenir des vecteurs de vaccin qui font preuve d'une meilleure efficacité lorsqu'on les utilise comme vaccins génétiques. Les vecteurs ainsi obtenus peuvent avoir, par exemple, une expression antigénique renforcée, une absorption dans la cellule améliorée, une meilleure stabilité dans la cellule, une capacité à concevoir une réponse immune et d'autres propriétés de ce genre. Cette invention concerne en outre des techniques permettant d'obtenir diverses molécules biologiques actives, dans le domaine des antibiotiques, des pharmacothérapeutiques, et des caractères transgéniques.
PCT/US2000/006497 1995-12-07 2000-03-09 Selection finale d'une evolution dirigee WO2000053744A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU38793/00A AU3879300A (en) 1999-03-09 2000-03-09 End selection in directed evolution
JP2000603365A JP2002537836A (ja) 1999-03-09 2000-03-09 定方向進化におけるエンドセレクション
CA002361927A CA2361927A1 (fr) 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee
IL14516500A IL145165A0 (en) 1999-03-09 2000-03-09 End selection in directed evolution
EP00917887A EP1161529A2 (fr) 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee
US10/151,469 US20030219752A1 (en) 1995-12-07 2002-05-17 Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
AU2005203719A AU2005203719A1 (en) 1999-03-09 2005-08-18 End selection in directed evolution

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US09/267,118 US6238884B1 (en) 1995-12-07 1999-03-09 End selection in directed evolution
US09/267,118 1999-03-09
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09/276,860 1999-03-26
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
US09/332,835 1999-06-14

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1997/012239 Continuation-In-Part WO1998001581A1 (fr) 1996-07-09 1997-07-09 Methode de remaniement d'adn avec des polynucleotides obtenus par blocage ou interruption d'un processus de synthese ou d'amplification
US10/151,469 Continuation-In-Part US20030219752A1 (en) 1995-12-07 2002-05-17 Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof

Publications (3)

Publication Number Publication Date
WO2000053744A2 WO2000053744A2 (fr) 2000-09-14
WO2000053744A3 true WO2000053744A3 (fr) 2001-01-18
WO2000053744A9 WO2000053744A9 (fr) 2001-06-28

Family

ID=27401940

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/006497 WO2000053744A2 (fr) 1995-12-07 2000-03-09 Selection finale d'une evolution dirigee

Country Status (6)

Country Link
EP (1) EP1161529A2 (fr)
JP (1) JP2002537836A (fr)
AU (1) AU3879300A (fr)
CA (1) CA2361927A1 (fr)
IL (1) IL145165A0 (fr)
WO (1) WO2000053744A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
CA2345203A1 (fr) 1998-10-07 2000-04-13 Maxygen Inc. Rearrangement d'adn pour produire des acides nucleiques de detoxication contre les mycotoxines
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
MXPA00009723A (es) * 1999-02-04 2003-09-22 Diversa Corp Generacion no estocastica de vacunas y enzimas geneticas.
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
CA2411828A1 (fr) 2000-06-23 2002-01-03 Maxygen, Inc. Nouvelles molecules costimulatrices
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP2002199890A (ja) * 2000-10-23 2002-07-16 Inst Of Physical & Chemical Res 生分解性ポリエステル合成酵素の改変方法
WO2002059357A2 (fr) * 2001-01-24 2002-08-01 Genomic Expression Aps Dosage et kit d'analyse d'expression genique
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
DK1356037T3 (da) 2001-01-25 2011-06-27 Evolva Ltd Bibliotek med en samling af celler
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1421203A4 (fr) * 2001-05-17 2005-06-01 Diversa Corp Nouvelles molecules de liaison a un antigene destinees a des applications therapeutiques, diagnostiques, prophylactiques, enzymatiques, industrielles et agricoles et procedes de generation et de criblage de telles molecules
WO2004073657A2 (fr) * 2003-02-19 2004-09-02 Protein Design Labs, Inc. Methode de diagnostic du cancer, composition et methodes de criblage destinees a identifier des modulateurs du cancer
WO2008054821A2 (fr) * 2006-10-30 2008-05-08 Promega Corporation Protéines hydrolase mutantes à cinétique et expression fonctionnelle améliorées
EP4368202A2 (fr) 2007-03-30 2024-05-15 The Research Foundation for The State University of New York Virus attenues utiles pour vaccins
WO2009088928A2 (fr) * 2007-12-31 2009-07-16 Xoma Technology Ltd. Librairies, réseaux et leurs utilisations pour une amélioration d'affinité ciblée
CN110498822A (zh) * 2019-09-04 2019-11-26 上海药明康德新药开发有限公司 DNA编码化合物库构建中on-DNA芳基叠氮化合物的合成方法

Citations (13)

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Publication number Priority date Publication date Assignee Title
EP0285123A2 (fr) * 1987-04-03 1988-10-05 Stabra AG Méthode de mutagénèse complète d'acides nucléiques
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
WO1995022625A1 (fr) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Mutagenese d'adn par fragmentation aleatoire et reassemblage
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO1998001581A1 (fr) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Methode de remaniement d'adn avec des polynucleotides obtenus par blocage ou interruption d'un processus de synthese ou d'amplification
WO1998013487A1 (fr) * 1996-09-27 1998-04-02 Maxygen, Inc. Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences
WO1998027230A1 (fr) * 1996-12-18 1998-06-25 Maxygen, Inc. Procedes et compositions pour l'ingenierie des polypeptides
WO1998032845A1 (fr) * 1997-01-24 1998-07-30 Bioinvent International Ab Procede d'evolution moleculaire in vitro de la fonction proteique
US5798208A (en) * 1990-04-05 1998-08-25 Roberto Crea Walk-through mutagenesis
WO1998042832A1 (fr) * 1997-03-25 1998-10-01 California Institute Of Technology Recombinaison de sequences de polynucleotides au moyen d'amorces aleatoires ou definies
US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
WO1998058080A1 (fr) * 1997-06-16 1998-12-23 Bioinvent International Ab Procede permettant l'evolution moleculaire in vitro d'une fonction proteique

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US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
EP0285123A2 (fr) * 1987-04-03 1988-10-05 Stabra AG Méthode de mutagénèse complète d'acides nucléiques
US5798208A (en) * 1990-04-05 1998-08-25 Roberto Crea Walk-through mutagenesis
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
WO1995022625A1 (fr) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Mutagenese d'adn par fragmentation aleatoire et reassemblage
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO1998001581A1 (fr) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Methode de remaniement d'adn avec des polynucleotides obtenus par blocage ou interruption d'un processus de synthese ou d'amplification
WO1998013487A1 (fr) * 1996-09-27 1998-04-02 Maxygen, Inc. Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences
WO1998027230A1 (fr) * 1996-12-18 1998-06-25 Maxygen, Inc. Procedes et compositions pour l'ingenierie des polypeptides
WO1998032845A1 (fr) * 1997-01-24 1998-07-30 Bioinvent International Ab Procede d'evolution moleculaire in vitro de la fonction proteique
WO1998042832A1 (fr) * 1997-03-25 1998-10-01 California Institute Of Technology Recombinaison de sequences de polynucleotides au moyen d'amorces aleatoires ou definies
WO1998058080A1 (fr) * 1997-06-16 1998-12-23 Bioinvent International Ab Procede permettant l'evolution moleculaire in vitro d'une fonction proteique

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RIECHMANN L AND WEILL M: "Phage display and selection of a site-directed randomized single-chain antibody Fv fragment for its affinity improvement", BIOCHEMISTRY, vol. 32, no. 34, 31 August 1993 (1993-08-31), pages 8848 - 8855, XP002147197 *
SCHULTZ S ET AL: "Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 83, no. 6, March 1986 (1986-03-01), pages 1588 - 1592, XP002147196 *
ZHAO H ET AL: "MOLECULAR EVOLUTION BY STAGGERED EXTENSION PROCESS (STEP) IN VITRO RECOMBINATION", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 16, 1 March 1998 (1998-03-01), pages 258 - 261, XP000775867, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
AU3879300A (en) 2000-09-28
CA2361927A1 (fr) 2000-09-14
JP2002537836A (ja) 2002-11-12
IL145165A0 (en) 2002-06-30
WO2000053744A2 (fr) 2000-09-14
EP1161529A2 (fr) 2001-12-12
WO2000053744A9 (fr) 2001-06-28

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