IL145165A0 - End selection in directed evolution - Google Patents
End selection in directed evolutionInfo
- Publication number
- IL145165A0 IL145165A0 IL14516500A IL14516500A IL145165A0 IL 145165 A0 IL145165 A0 IL 145165A0 IL 14516500 A IL14516500 A IL 14516500A IL 14516500 A IL14516500 A IL 14516500A IL 145165 A0 IL145165 A0 IL 145165A0
- Authority
- IL
- Israel
- Prior art keywords
- directed evolution
- end selection
- selection
- evolution
- directed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/267,118 US6238884B1 (en) | 1995-12-07 | 1999-03-09 | End selection in directed evolution |
US09/276,860 US6352842B1 (en) | 1995-12-07 | 1999-03-26 | Exonucease-mediated gene assembly in directed evolution |
US09/332,835 US6537776B1 (en) | 1999-06-14 | 1999-06-14 | Synthetic ligation reassembly in directed evolution |
PCT/US2000/006497 WO2000053744A2 (fr) | 1999-03-09 | 2000-03-09 | Selection finale d'une evolution dirigee |
Publications (1)
Publication Number | Publication Date |
---|---|
IL145165A0 true IL145165A0 (en) | 2002-06-30 |
Family
ID=27401940
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL14516500A IL145165A0 (en) | 1999-03-09 | 2000-03-09 | End selection in directed evolution |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1161529A2 (fr) |
JP (1) | JP2002537836A (fr) |
AU (1) | AU3879300A (fr) |
CA (1) | CA2361927A1 (fr) |
IL (1) | IL145165A0 (fr) |
WO (1) | WO2000053744A2 (fr) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
EP1119616A2 (fr) | 1998-10-07 | 2001-08-01 | Maxygen, Inc. | Rearrangement d'adn pour produire des acides nucleiques de detoxication contre les mycotoxines |
US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
EP1073710A4 (fr) * | 1999-02-04 | 2007-12-19 | Verenium Corp | Elaboration non stochastique de vaccins genetiques et d'enzymes |
US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
WO2002000717A2 (fr) | 2000-06-23 | 2002-01-03 | Maxygen, Inc. | Nouvelles molecules costimulatrices |
US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
JP2002199890A (ja) * | 2000-10-23 | 2002-07-16 | Inst Of Physical & Chemical Res | 生分解性ポリエステル合成酵素の改変方法 |
US6958217B2 (en) | 2001-01-24 | 2005-10-25 | Genomic Expression Aps | Single-stranded polynucleotide tags |
WO2002059357A2 (fr) * | 2001-01-24 | 2002-08-01 | Genomic Expression Aps | Dosage et kit d'analyse d'expression genique |
US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
WO2002059297A2 (fr) | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | Échantillothèque pour collection de cellules |
EP1421203A4 (fr) * | 2001-05-17 | 2005-06-01 | Diversa Corp | Nouvelles molecules de liaison a un antigene destinees a des applications therapeutiques, diagnostiques, prophylactiques, enzymatiques, industrielles et agricoles et procedes de generation et de criblage de telles molecules |
WO2004073657A2 (fr) * | 2003-02-19 | 2004-09-02 | Protein Design Labs, Inc. | Methode de diagnostic du cancer, composition et methodes de criblage destinees a identifier des modulateurs du cancer |
EP2492342B1 (fr) | 2006-10-30 | 2015-06-03 | Promega Corporation | Protéines d'hydrolase mutante avec expressions cinétique et fonctionnelle améliorées |
US9476032B2 (en) | 2007-03-30 | 2016-10-25 | The Research Foundation For The State University Of New York | Attenuated viruses useful for vaccines |
DK2242843T3 (en) * | 2007-12-31 | 2015-07-20 | Xoma Technology Ltd | Methods and materials for targeted mutagenesis |
CN110498822A (zh) * | 2019-09-04 | 2019-11-26 | 上海药明康德新药开发有限公司 | DNA编码化合物库构建中on-DNA芳基叠氮化合物的合成方法 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5824469A (en) * | 1986-07-17 | 1998-10-20 | University Of Washington | Method for producing novel DNA sequences with biological activity |
EP0285123A3 (fr) * | 1987-04-03 | 1989-02-01 | Stabra AG | Méthode de mutagénèse complète d'acides nucléiques |
DE69112207T2 (de) * | 1990-04-05 | 1996-03-28 | Roberto Chestnut Hill Mass. Crea | "walk-through"-mutagenese. |
US5512463A (en) * | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
US5223408A (en) * | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5965408A (en) * | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
EP0964922A4 (fr) * | 1996-09-27 | 2000-10-25 | Maxygen Inc | Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences |
GB9701425D0 (en) * | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
GB9712512D0 (en) * | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
-
2000
- 2000-03-09 EP EP00917887A patent/EP1161529A2/fr not_active Withdrawn
- 2000-03-09 CA CA002361927A patent/CA2361927A1/fr not_active Abandoned
- 2000-03-09 IL IL14516500A patent/IL145165A0/xx unknown
- 2000-03-09 WO PCT/US2000/006497 patent/WO2000053744A2/fr not_active Application Discontinuation
- 2000-03-09 JP JP2000603365A patent/JP2002537836A/ja active Pending
- 2000-03-09 AU AU38793/00A patent/AU3879300A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2000053744A2 (fr) | 2000-09-14 |
JP2002537836A (ja) | 2002-11-12 |
CA2361927A1 (fr) | 2000-09-14 |
WO2000053744A3 (fr) | 2001-01-18 |
EP1161529A2 (fr) | 2001-12-12 |
AU3879300A (en) | 2000-09-28 |
WO2000053744A9 (fr) | 2001-06-28 |
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