AU3879300A - End selection in directed evolution - Google Patents

End selection in directed evolution Download PDF

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Publication number
AU3879300A
AU3879300A AU38793/00A AU3879300A AU3879300A AU 3879300 A AU3879300 A AU 3879300A AU 38793/00 A AU38793/00 A AU 38793/00A AU 3879300 A AU3879300 A AU 3879300A AU 3879300 A AU3879300 A AU 3879300A
Authority
AU
Australia
Prior art keywords
yes
sequence
polynucleotide
sequences
neg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU38793/00A
Other languages
English (en)
Inventor
Gerhard Johann Frey
Jay M. Short
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/267,118 external-priority patent/US6238884B1/en
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of AU3879300A publication Critical patent/AU3879300A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU38793/00A 1999-03-09 2000-03-09 End selection in directed evolution Abandoned AU3879300A (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US09/267,118 US6238884B1 (en) 1995-12-07 1999-03-09 End selection in directed evolution
US09267118 1999-03-09
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09276860 1999-03-26
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
US09332835 1999-06-14
PCT/US2000/006497 WO2000053744A2 (fr) 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2005203719A Division AU2005203719A1 (en) 1999-03-09 2005-08-18 End selection in directed evolution

Publications (1)

Publication Number Publication Date
AU3879300A true AU3879300A (en) 2000-09-28

Family

ID=27401940

Family Applications (1)

Application Number Title Priority Date Filing Date
AU38793/00A Abandoned AU3879300A (en) 1999-03-09 2000-03-09 End selection in directed evolution

Country Status (6)

Country Link
EP (1) EP1161529A2 (fr)
JP (1) JP2002537836A (fr)
AU (1) AU3879300A (fr)
CA (1) CA2361927A1 (fr)
IL (1) IL145165A0 (fr)
WO (1) WO2000053744A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
CA2345203A1 (fr) 1998-10-07 2000-04-13 Maxygen Inc. Rearrangement d'adn pour produire des acides nucleiques de detoxication contre les mycotoxines
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
MXPA00009723A (es) * 1999-02-04 2003-09-22 Diversa Corp Generacion no estocastica de vacunas y enzimas geneticas.
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
JP2004513878A (ja) 2000-06-23 2004-05-13 マキシジェン，　インコーポレイテッド 新規同時刺激分子
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP2002199890A (ja) * 2000-10-23 2002-07-16 Inst Of Physical & Chemical Res 生分解性ポリエステル合成酵素の改変方法
CA2473308C (fr) * 2001-01-24 2013-12-03 Genomic Expression Aps Dosage et kit d'analyse d'expression genique
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
CA2474172C (fr) 2001-01-25 2012-07-10 Evolva Ltd. Genotheque cellulaire
JP2004532038A (ja) * 2001-05-17 2004-10-21 ディヴァーサ コーポレイション 新規抗原結合分子の治療、診断、予防、酵素、産業ならびに農業各分野への応用とそのための新規抗原結合分子の作製とスクリーニングの方法
WO2004073657A2 (fr) * 2003-02-19 2004-09-02 Protein Design Labs, Inc. Methode de diagnostic du cancer, composition et methodes de criblage destinees a identifier des modulateurs du cancer
US8420367B2 (en) * 2006-10-30 2013-04-16 Promega Corporation Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression
CA2682089C (fr) 2007-03-30 2019-03-12 The Research Foundation Of State University Of New York Virus attenues utiles pour des vaccins
EP2242843B1 (fr) * 2007-12-31 2015-05-27 XOMA Technology Ltd. Procédés et matériaux pour mutagenèse ciblée
CN110498822A (zh) * 2019-09-04 2019-11-26 上海药明康德新药开发有限公司 DNA编码化合物库构建中on-DNA芳基叠氮化合物的合成方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
EP0285123A3 (fr) * 1987-04-03 1989-02-01 Stabra AG Méthode de mutagénèse complète d'acides nucléiques
DE69112207T2 (de) * 1990-04-05 1996-03-28 Roberto Crea "walk-through"-mutagenese.
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
JP2001504325A (ja) * 1996-09-27 2001-04-03 マキシジェン，インコーポレイテッド 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function

Also Published As

Publication number Publication date
WO2000053744A3 (fr) 2001-01-18
IL145165A0 (en) 2002-06-30
CA2361927A1 (fr) 2000-09-14
WO2000053744A9 (fr) 2001-06-28
WO2000053744A2 (fr) 2000-09-14
JP2002537836A (ja) 2002-11-12
EP1161529A2 (fr) 2001-12-12

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