JP2002537836A - 定方向進化におけるエンドセレクション - Google Patents

定方向進化におけるエンドセレクション

Info

Publication number
JP2002537836A
JP2002537836A JP2000603365A JP2000603365A JP2002537836A JP 2002537836 A JP2002537836 A JP 2002537836A JP 2000603365 A JP2000603365 A JP 2000603365A JP 2000603365 A JP2000603365 A JP 2000603365A JP 2002537836 A JP2002537836 A JP 2002537836A
Authority
JP
Japan
Prior art keywords
polynucleotide
sequence
sequences
nucleic acid
mutagenesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000603365A
Other languages
English (en)
Japanese (ja)
Inventor
ジェイ エム. ショート
ゲルハード ジョハン フレイ
Original Assignee
ディベルサ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/267,118 external-priority patent/US6238884B1/en
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by ディベルサ コーポレーション filed Critical ディベルサ コーポレーション
Publication of JP2002537836A publication Critical patent/JP2002537836A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2000603365A 1999-03-09 2000-03-09 定方向進化におけるエンドセレクション Pending JP2002537836A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US09/267,118 US6238884B1 (en) 1995-12-07 1999-03-09 End selection in directed evolution
US09/267,118 1999-03-09
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09/276,860 1999-06-14
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
US09/332,835 1999-06-14
PCT/US2000/006497 WO2000053744A2 (fr) 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee

Publications (1)

Publication Number Publication Date
JP2002537836A true JP2002537836A (ja) 2002-11-12

Family

ID=27401940

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000603365A Pending JP2002537836A (ja) 1999-03-09 2000-03-09 定方向進化におけるエンドセレクション

Country Status (6)

Country Link
EP (1) EP1161529A2 (fr)
JP (1) JP2002537836A (fr)
AU (1) AU3879300A (fr)
CA (1) CA2361927A1 (fr)
IL (1) IL145165A0 (fr)
WO (1) WO2000053744A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010508023A (ja) * 2006-10-30 2010-03-18 プロメガ コーポレイション キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
JP2002526107A (ja) 1998-10-07 2002-08-20 マキシジェン, インコーポレイテッド マイコトキシンの解毒のための核酸を生成するためのdnaシャッフリング
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
IL138206A (en) * 1999-02-04 2011-06-30 Verenium Corp Methods for non-stochastic generation of progeny polypeptides and hybrid polynucleotides
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7094875B2 (en) 2000-06-23 2006-08-22 Maxygen, Inc. Co-stimulatory polypeptides
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP2002199890A (ja) * 2000-10-23 2002-07-16 Inst Of Physical & Chemical Res 生分解性ポリエステル合成酵素の改変方法
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
WO2002059357A2 (fr) * 2001-01-24 2002-08-01 Genomic Expression Aps Dosage et kit d'analyse d'expression genique
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DK1356037T3 (da) 2001-01-25 2011-06-27 Evolva Ltd Bibliotek med en samling af celler
WO2002092780A2 (fr) * 2001-05-17 2002-11-21 Diversa Corporation Nouvelles molecules de liaison a un antigene destinees a des applications therapeutiques, diagnostiques, prophylactiques, enzymatiques, industrielles et agricoles et procedes de generation et de criblage de telles molecules
WO2004073657A2 (fr) * 2003-02-19 2004-09-02 Protein Design Labs, Inc. Methode de diagnostic du cancer, composition et methodes de criblage destinees a identifier des modulateurs du cancer
HRP20181319T4 (hr) 2007-03-30 2024-02-16 The Research Foundation Of The State University Of New York Oslabljeni virusi koji su korisni kao cjepiva
EP2980217A1 (fr) * 2007-12-31 2016-02-03 XOMA Technology Ltd. Procédés et matériaux pour mutagenèse ciblée
CN110498822A (zh) * 2019-09-04 2019-11-26 上海药明康德新药开发有限公司 DNA编码化合物库构建中on-DNA芳基叠氮化合物的合成方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
EP0285123A3 (fr) * 1987-04-03 1989-02-01 Stabra AG Méthode de mutagénèse complète d'acides nucléiques
EP0527809B1 (fr) * 1990-04-05 1995-08-16 CREA, Roberto Mutagenese "traversante"
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1998013487A1 (fr) * 1996-09-27 1998-04-02 Maxygen, Inc. Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010508023A (ja) * 2006-10-30 2010-03-18 プロメガ コーポレイション キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質
JP2013078329A (ja) * 2006-10-30 2013-05-02 Promega Corp キネティクス及び機能発現が高められた突然変異形ヒドロラーゼ・タンパク質
US8748148B2 (en) 2006-10-30 2014-06-10 Promega Corporation Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression
US9593316B2 (en) 2006-10-30 2017-03-14 Promega Corporation Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression
US9873866B2 (en) 2006-10-30 2018-01-23 Promega Corporation Mutant dehalogenase proteins
US10246690B2 (en) 2006-10-30 2019-04-02 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression

Also Published As

Publication number Publication date
EP1161529A2 (fr) 2001-12-12
CA2361927A1 (fr) 2000-09-14
IL145165A0 (en) 2002-06-30
AU3879300A (en) 2000-09-28
WO2000053744A3 (fr) 2001-01-18
WO2000053744A2 (fr) 2000-09-14
WO2000053744A9 (fr) 2001-06-28

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