CA2361927A1 - Selection finale d'une evolution dirigee - Google Patents

Selection finale d'une evolution dirigee Download PDF

Info

Publication number
CA2361927A1
CA2361927A1 CA002361927A CA2361927A CA2361927A1 CA 2361927 A1 CA2361927 A1 CA 2361927A1 CA 002361927 A CA002361927 A CA 002361927A CA 2361927 A CA2361927 A CA 2361927A CA 2361927 A1 CA2361927 A1 CA 2361927A1
Authority
CA
Canada
Prior art keywords
yes
sequence
polynucleotide
progeny
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002361927A
Other languages
English (en)
Inventor
Jay M. Short
Gerhard Johann Frey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/267,118 external-priority patent/US6238884B1/en
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Individual filed Critical Individual
Publication of CA2361927A1 publication Critical patent/CA2361927A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Cette invention concerne des techniques permettant d'obtenir des polynucléotides et des polynucléotides codés en utilisant des techniques non stochastiques d'évolution dirigées (DirectEvolution™). La possibilité de récupérer des polynucléotides de pleine longueur issus d'une bibliothèque de molécules d'une lignée générées par mutagenèse présente un avantage particulier dans les techniques fondées sur une sélection finale. Ces techniques comprennent des mutagenèses à saturation de site non stochastiques de polynucléotides (Gene Site Saturation Mutagenesis™) et un réassemblage de polynucléotides non stochastique (GeneReassembly™). Cette invention concerne des techniques permettant d'obtenir des enzymes aux propriétés physiques et/ou biologiques optimisées. Par l'utilisation de ces techniques de l'invention, on peut faire évoluer des vaccins génétiques, des enzymes, de petites molécules et d'autres molécules qui conviennent vers les propriétés souhaitées. On peut, par exemple, obtenir des vecteurs de vaccin qui font preuve d'une meilleure efficacité lorsqu'on les utilise comme vaccins génétiques. Les vecteurs ainsi obtenus peuvent avoir, par exemple, une expression antigénique renforcée, une absorption dans la cellule améliorée, une meilleure stabilité dans la cellule, une capacité à concevoir une réponse immune et d'autres propriétés de ce genre. Cette invention concerne en outre des techniques permettant d'obtenir diverses molécules biologiques actives, dans le domaine des antibiotiques, des pharmacothérapeutiques, et des caractères transgéniques.
CA002361927A 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee Abandoned CA2361927A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US09/267,118 US6238884B1 (en) 1995-12-07 1999-03-09 End selection in directed evolution
US09/267,118 1999-03-09
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09/276,860 1999-06-14
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
US09/332,835 1999-06-14
PCT/US2000/006497 WO2000053744A2 (fr) 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee

Publications (1)

Publication Number Publication Date
CA2361927A1 true CA2361927A1 (fr) 2000-09-14

Family

ID=27401940

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002361927A Abandoned CA2361927A1 (fr) 1999-03-09 2000-03-09 Selection finale d'une evolution dirigee

Country Status (6)

Country Link
EP (1) EP1161529A2 (fr)
JP (1) JP2002537836A (fr)
AU (1) AU3879300A (fr)
CA (1) CA2361927A1 (fr)
IL (1) IL145165A0 (fr)
WO (1) WO2000053744A2 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
JP2002526107A (ja) 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド マイコトキシンの解毒のための核酸を生成するためのｄｎａシャッフリング
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
IL138206A (en) * 1999-02-04 2011-06-30 Verenium Corp Methods for non-stochastic generation of progeny polypeptides and hybrid polynucleotides
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7094875B2 (en) 2000-06-23 2006-08-22 Maxygen, Inc. Co-stimulatory polypeptides
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP2002199890A (ja) * 2000-10-23 2002-07-16 Inst Of Physical & Chemical Res 生分解性ポリエステル合成酵素の改変方法
US6958217B2 (en) 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
WO2002059357A2 (fr) * 2001-01-24 2002-08-01 Genomic Expression Aps Dosage et kit d'analyse d'expression genique
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DK1356037T3 (da) 2001-01-25 2011-06-27 Evolva Ltd Bibliotek med en samling af celler
WO2002092780A2 (fr) * 2001-05-17 2002-11-21 Diversa Corporation Nouvelles molecules de liaison a un antigene destinees a des applications therapeutiques, diagnostiques, prophylactiques, enzymatiques, industrielles et agricoles et procedes de generation et de criblage de telles molecules
WO2004073657A2 (fr) * 2003-02-19 2004-09-02 Protein Design Labs, Inc. Methode de diagnostic du cancer, composition et methodes de criblage destinees a identifier des modulateurs du cancer
EP2374875B1 (fr) 2006-10-30 2015-09-02 Promega Corporation Protéines d'hydrolase mutante avec expressions cinétique et fonctionnelle améliorées
HRP20181319T4 (hr) 2007-03-30 2024-02-16 The Research Foundation Of The State University Of New York Oslabljeni virusi koji su korisni kao cjepiva
EP2980217A1 (fr) * 2007-12-31 2016-02-03 XOMA Technology Ltd. Procédés et matériaux pour mutagenèse ciblée
CN110498822A (zh) * 2019-09-04 2019-11-26 上海药明康德新药开发有限公司 DNA编码化合物库构建中on-DNA芳基叠氮化合物的合成方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
EP0285123A3 (fr) * 1987-04-03 1989-02-01 Stabra AG Méthode de mutagénèse complète d'acides nucléiques
EP0527809B1 (fr) * 1990-04-05 1995-08-16 CREA, Roberto Mutagenese "traversante"
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1998013487A1 (fr) * 1996-09-27 1998-04-02 Maxygen, Inc. Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function

Also Published As

Publication number Publication date
EP1161529A2 (fr) 2001-12-12
IL145165A0 (en) 2002-06-30
AU3879300A (en) 2000-09-28
WO2000053744A3 (fr) 2001-01-18
WO2000053744A2 (fr) 2000-09-14
JP2002537836A (ja) 2002-11-12
WO2000053744A9 (fr) 2001-06-28

Similar Documents

Publication Publication Date Title
CA2374667C (fr) Reassemblage synthetique par ligature a evolution dirigee
US6773900B2 (en) End selection in directed evolution
US6713282B2 (en) End selection in directed evolution
US6635449B2 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
US6352842B1 (en) Exonucease-mediated gene assembly in directed evolution
US6696275B2 (en) End selection in directed evolution
AU4039400A (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
CA2361927A1 (fr) Selection finale d'une evolution dirigee
US6939689B2 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
AU2009212959B2 (en) Synthetic ligation reassembly in directed evolution
US20040023327A1 (en) End selection in directed evolution
AU2005203018A1 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
Class et al. Patent application title: SYNTHETIC LIGATION REASSEMBLY IN DIRECTED EVOLUTION Inventors: Jay M. Short (Del Mar, CA, US)
AU2005203719A1 (en) End selection in directed evolution

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued