WO2000033880A2 - Conjugue pour l'administration dans des cellules neuronales - Google Patents

Conjugue pour l'administration dans des cellules neuronales Download PDF

Info

Publication number
WO2000033880A2
WO2000033880A2 PCT/DE1999/003924 DE9903924W WO0033880A2 WO 2000033880 A2 WO2000033880 A2 WO 2000033880A2 DE 9903924 W DE9903924 W DE 9903924W WO 0033880 A2 WO0033880 A2 WO 0033880A2
Authority
WO
WIPO (PCT)
Prior art keywords
toxin
heavy chain
conjugate according
protein
conjugate
Prior art date
Application number
PCT/DE1999/003924
Other languages
German (de)
English (en)
Other versions
WO2000033880A3 (fr
Inventor
Achim LÖHR
Manfred Schwab
Original Assignee
Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts filed Critical Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts
Publication of WO2000033880A2 publication Critical patent/WO2000033880A2/fr
Publication of WO2000033880A3 publication Critical patent/WO2000033880A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/6415—Toxins or lectins, e.g. clostridial toxins or Pseudomonas exotoxins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Organic Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un conjugué utilisé pour introduire des principes actifs dans des cellules neuronales. Ce conjugué comprend la chaîne lourde d'une toxine de l'espèce Clostridium et au moins un principe actif, la chaîne légère de la toxine étant absente. L'invention concerne en outre un procédé de production d'un tel conjugué, une trousse associée et l'utilisation dudit conjugué.
PCT/DE1999/003924 1998-12-04 1999-12-03 Conjugue pour l'administration dans des cellules neuronales WO2000033880A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19856052.4 1998-12-04
DE19856052A DE19856052A1 (de) 1998-12-04 1998-12-04 Konjugat zur Anreicherung in neuronalen Zellen

Publications (2)

Publication Number Publication Date
WO2000033880A2 true WO2000033880A2 (fr) 2000-06-15
WO2000033880A3 WO2000033880A3 (fr) 2000-10-12

Family

ID=7890019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/003924 WO2000033880A2 (fr) 1998-12-04 1999-12-03 Conjugue pour l'administration dans des cellules neuronales

Country Status (2)

Country Link
DE (1) DE19856052A1 (fr)
WO (1) WO2000033880A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004071525A1 (fr) * 2002-02-08 2004-08-26 Allergan, Inc. Methodes de traitement de troubles des glandes mammaires
WO2006010360A2 (fr) * 2004-07-22 2006-02-02 Biotecon Therapeutics Gmbh Vehicule permettant d'obtenir des medicaments a biodisponibilite orale
WO2009083738A2 (fr) * 2007-12-31 2009-07-09 Syntaxin Limited Véhicules de délivrance d'arn
US7628992B1 (en) 1994-05-31 2009-12-08 Invent Dcu Limited Modification of clostridial toxins for use as transport proteins
US7838008B2 (en) 1999-12-07 2010-11-23 Allergan, Inc. Methods for treating diverse cancers

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
EP2418281B1 (fr) * 2003-10-24 2016-06-01 Gencia Corporation Procédés et compositions pour l'administration de polynucléotides
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4594336A (en) * 1982-09-28 1986-06-10 Institut Pasteur Thiolated polypeptide compound derived from a tetanus toxin fragment, the process for obtaining and its application
WO1989006974A2 (fr) * 1988-02-01 1989-08-10 Praxis Biologics, Inc. Epitopes de cellules t a titre de molecules porteuses pour vaccins conjugues
WO1995032738A1 (fr) * 1994-05-31 1995-12-07 Allergan, Inc. Modification de toxines de clostridium utilisees comme proteines de transport
WO1997020579A2 (fr) * 1995-12-05 1997-06-12 Smithkline Beecham Plc Nouveaux composes et leur utilisation
WO2000028041A1 (fr) * 1998-11-05 2000-05-18 Microbiological Research Authority Apport de superoxyde-dismutase aux cellules neuronales

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4594336A (en) * 1982-09-28 1986-06-10 Institut Pasteur Thiolated polypeptide compound derived from a tetanus toxin fragment, the process for obtaining and its application
WO1989006974A2 (fr) * 1988-02-01 1989-08-10 Praxis Biologics, Inc. Epitopes de cellules t a titre de molecules porteuses pour vaccins conjugues
WO1995032738A1 (fr) * 1994-05-31 1995-12-07 Allergan, Inc. Modification de toxines de clostridium utilisees comme proteines de transport
WO1997020579A2 (fr) * 1995-12-05 1997-06-12 Smithkline Beecham Plc Nouveaux composes et leur utilisation
WO2000028041A1 (fr) * 1998-11-05 2000-05-18 Microbiological Research Authority Apport de superoxyde-dismutase aux cellules neuronales

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FRANCIS JW ET AL: "CuZn superoxide dismutase (SOD-1): tetanus toxin fragment C hybrid protein for targeted delivery of SOD-1 to neuronal cells" JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, Bd. 270, Nr. 25, 23. Juni 1995 (1995-06-23), Seiten 15434-15442-15442, XP002131795 ISSN: 0021-9258 *
JOHNSTONE, STEPHEN R. ET AL: "The heavy chain of tetanus toxin can mediat the entry of cytotoxic gelonin into intact cells" FEBS LETT. (1990), 265(1-2), 101-3 , XP002140049 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7628992B1 (en) 1994-05-31 2009-12-08 Invent Dcu Limited Modification of clostridial toxins for use as transport proteins
US7838008B2 (en) 1999-12-07 2010-11-23 Allergan, Inc. Methods for treating diverse cancers
US7838007B2 (en) 1999-12-07 2010-11-23 Allergan, Inc. Methods for treating mammary gland disorders
US7846456B2 (en) 1999-12-07 2010-12-07 Allergan, Inc. Methods for breast treatment
WO2004071525A1 (fr) * 2002-02-08 2004-08-26 Allergan, Inc. Methodes de traitement de troubles des glandes mammaires
JP2006510723A (ja) * 2002-02-08 2006-03-30 アラーガン、インコーポレイテッド 乳腺障害の処置方法
EP2204183A1 (fr) * 2002-02-08 2010-07-07 Allergan, Inc. Traitement du neuroblastome
WO2006010360A2 (fr) * 2004-07-22 2006-02-02 Biotecon Therapeutics Gmbh Vehicule permettant d'obtenir des medicaments a biodisponibilite orale
WO2006010360A3 (fr) * 2004-07-22 2007-12-27 Biotecon Therapeutics Gmbh Vehicule permettant d'obtenir des medicaments a biodisponibilite orale
WO2009083738A2 (fr) * 2007-12-31 2009-07-09 Syntaxin Limited Véhicules de délivrance d'arn
WO2009083738A3 (fr) * 2007-12-31 2009-08-27 Syntaxin Limited Véhicules de délivrance d'arn

Also Published As

Publication number Publication date
WO2000033880A3 (fr) 2000-10-12
DE19856052A1 (de) 2000-06-08

Similar Documents

Publication Publication Date Title
DE69827507T2 (de) Trimerisierendes modul
DE69635653T2 (de) Peptide als vektoren zur intrazellulären adressierung von aktiven molekülen
DE69924870T2 (de) Peptidvektoren von Stoffen, die durch die Blut-Hirn-Schranke dringen
EP1379546B1 (fr) Peptide servant au diagnostic et a la therapie de la maladie d'alzheimer
CH688195A5 (de) Peptide mit PTH-aehnlicher Aktivitaet.
EP0571414B1 (fr) Nouveaux complexes contenant de l'acide nucleique absorbables par endocytose par des cellules eucaryotes superieures
WO1994023751A1 (fr) Peptides de tranfert d'acides nucleiques et leur utilisation pour injecter des acides nucleiques dans des cellules eucaryotes
JPH04506664A (ja) 治療用ペプチド
DE60130324T2 (de) Von humanen proteinen, welche mit heparin reagieren, abgeleitete aminosäuresequenzen, die den transfer von substanzen von interesse in das innere von zellen und/oder zellkernen erleichtern
DE60008915T2 (de) Konstrukte zur verabreichung von therapeutischen wirkstoffen an die neuronzellen
EP2167139A2 (fr) Composé diagnostique et thérapeutique activable
WO2000033880A2 (fr) Conjugue pour l'administration dans des cellules neuronales
EP1630171B1 (fr) Peptides qui lient streptavidine
DE60019948T2 (de) Humanin, ein Polypeptid, welches neuronales Absterben unterdrückt
JP2004517039A (ja) 両親媒性線状ペプチド及びこれらを含有する化合物
DE10131145B4 (de) Zusammensetzung zum zellspezifischen Transfer von Wirkstoffen
WO2000042176A1 (fr) Selection d'anticorps monoclonaux
DE19850718C1 (de) Zellpermeabilität-vermittelndes Polypeptid
KR102274876B1 (ko) 신규한 세포 투과성 펩타이드 및 이의 용도
CN109562144A (zh) 胰高血糖素类似物及其使用方法
WO1999040109A2 (fr) Lectines de gui recombinees
JP2002534996A (ja) アルファ−コノトキシン・ペプチド
DE112018006444T5 (de) pH-low-Insertionspeptid und Zusammensetzung davon
KR102274877B1 (ko) 신규한 세포 투과성 펩타이드 및 이의 용도
WO2017087879A2 (fr) Compositions et procédé de traitement et de dépistage du cancer du côlon

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

122 Ep: pct application non-entry in european phase