WO2000033880A3 - Conjugue pour l'administration dans des cellules neuronales - Google Patents

Conjugue pour l'administration dans des cellules neuronales Download PDF

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Publication number
WO2000033880A3
WO2000033880A3 PCT/DE1999/003924 DE9903924W WO0033880A3 WO 2000033880 A3 WO2000033880 A3 WO 2000033880A3 DE 9903924 W DE9903924 W DE 9903924W WO 0033880 A3 WO0033880 A3 WO 0033880A3
Authority
WO
WIPO (PCT)
Prior art keywords
conjugate
neuronal cells
enriching
conjugate used
toxin
Prior art date
Application number
PCT/DE1999/003924
Other languages
German (de)
English (en)
Other versions
WO2000033880A2 (fr
Inventor
Achim Loehr
Manfred Schwab
Original Assignee
Deutsches Krebsforsch
Achim Loehr
Manfred Schwab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforsch, Achim Loehr, Manfred Schwab filed Critical Deutsches Krebsforsch
Publication of WO2000033880A2 publication Critical patent/WO2000033880A2/fr
Publication of WO2000033880A3 publication Critical patent/WO2000033880A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/6415—Toxins or lectins, e.g. clostridial toxins or Pseudomonas exotoxins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un conjugué utilisé pour introduire des principes actifs dans des cellules neuronales. Ce conjugué comprend la chaîne lourde d'une toxine de l'espèce Clostridium et au moins un principe actif, la chaîne légère de la toxine étant absente. L'invention concerne en outre un procédé de production d'un tel conjugué, une trousse associée et l'utilisation dudit conjugué.
PCT/DE1999/003924 1998-12-04 1999-12-03 Conjugue pour l'administration dans des cellules neuronales WO2000033880A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19856052.4 1998-12-04
DE19856052A DE19856052A1 (de) 1998-12-04 1998-12-04 Konjugat zur Anreicherung in neuronalen Zellen

Publications (2)

Publication Number Publication Date
WO2000033880A2 WO2000033880A2 (fr) 2000-06-15
WO2000033880A3 true WO2000033880A3 (fr) 2000-10-12

Family

ID=7890019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/003924 WO2000033880A2 (fr) 1998-12-04 1999-12-03 Conjugue pour l'administration dans des cellules neuronales

Country Status (2)

Country Link
DE (1) DE19856052A1 (fr)
WO (1) WO2000033880A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9410871D0 (en) 1994-05-31 1994-07-20 Imperial College Modification of tetanus toxin for use as a transport protein
US7838007B2 (en) * 1999-12-07 2010-11-23 Allergan, Inc. Methods for treating mammary gland disorders
US7838008B2 (en) 1999-12-07 2010-11-23 Allergan, Inc. Methods for treating diverse cancers
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US8039587B2 (en) 2003-10-24 2011-10-18 Gencia Corporation Methods and compositions for delivering polynucleotides
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
DE102004035606A1 (de) * 2004-07-22 2006-03-30 Biotecon Therapeutics Gmbh Carrier für Arzneimittel zur Gewinnung der oralen Bioverfügbarkeit
GB0725321D0 (en) * 2007-12-31 2008-02-06 Syntaxin Ltd Delivery vehicles

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4594336A (en) * 1982-09-28 1986-06-10 Institut Pasteur Thiolated polypeptide compound derived from a tetanus toxin fragment, the process for obtaining and its application
WO1989006974A2 (fr) * 1988-02-01 1989-08-10 Praxis Biologics, Inc. Epitopes de cellules t a titre de molecules porteuses pour vaccins conjugues
WO1995032738A1 (fr) * 1994-05-31 1995-12-07 Allergan, Inc. Modification de toxines de clostridium utilisees comme proteines de transport
WO1997020579A2 (fr) * 1995-12-05 1997-06-12 Smithkline Beecham Plc Nouveaux composes et leur utilisation
WO2000028041A1 (fr) * 1998-11-05 2000-05-18 Microbiological Research Authority Apport de superoxyde-dismutase aux cellules neuronales

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4594336A (en) * 1982-09-28 1986-06-10 Institut Pasteur Thiolated polypeptide compound derived from a tetanus toxin fragment, the process for obtaining and its application
WO1989006974A2 (fr) * 1988-02-01 1989-08-10 Praxis Biologics, Inc. Epitopes de cellules t a titre de molecules porteuses pour vaccins conjugues
WO1995032738A1 (fr) * 1994-05-31 1995-12-07 Allergan, Inc. Modification de toxines de clostridium utilisees comme proteines de transport
WO1997020579A2 (fr) * 1995-12-05 1997-06-12 Smithkline Beecham Plc Nouveaux composes et leur utilisation
WO2000028041A1 (fr) * 1998-11-05 2000-05-18 Microbiological Research Authority Apport de superoxyde-dismutase aux cellules neuronales

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FRANCIS JW ET AL: "CuZn superoxide dismutase (SOD-1): tetanus toxin fragment C hybrid protein for targeted delivery of SOD-1 to neuronal cells", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 270, no. 25, 23 June 1995 (1995-06-23), pages 15434 - 15442-15442, XP002131795, ISSN: 0021-9258 *
JOHNSTONE, STEPHEN R. ET AL: "The heavy chain of tetanus toxin can mediat the entry of cytotoxic gelonin into intact cells", FEBS LETT. (1990), 265(1-2), 101-3, XP002140049 *

Also Published As

Publication number Publication date
DE19856052A1 (de) 2000-06-08
WO2000033880A2 (fr) 2000-06-15

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