WO1999042827A3 - Vorrichtung zur detektion von oligo- und/oder polynukleotid-hybridisierungen - Google Patents

Vorrichtung zur detektion von oligo- und/oder polynukleotid-hybridisierungen Download PDF

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Publication number
WO1999042827A3
WO1999042827A3 PCT/DE1999/000460 DE9900460W WO9942827A3 WO 1999042827 A3 WO1999042827 A3 WO 1999042827A3 DE 9900460 W DE9900460 W DE 9900460W WO 9942827 A3 WO9942827 A3 WO 9942827A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide hybridization
detecting oligonucleotide
oligonucleotide
polynucleotide
detection
Prior art date
Application number
PCT/DE1999/000460
Other languages
English (en)
French (fr)
Other versions
WO1999042827A2 (de
Inventor
Vladimir Mirsky
Michael Riepl
Original Assignee
Wolfbeis Otto Samuel
Vladimir Mirsky
Michael Riepl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19807338A external-priority patent/DE19807338A1/de
Priority claimed from DE1998107339 external-priority patent/DE19807339A1/de
Application filed by Wolfbeis Otto Samuel, Vladimir Mirsky, Michael Riepl filed Critical Wolfbeis Otto Samuel
Priority to EP99915484A priority Critical patent/EP1055004A2/de
Publication of WO1999042827A2 publication Critical patent/WO1999042827A2/de
Publication of WO1999042827A3 publication Critical patent/WO1999042827A3/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/22—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
    • G01N27/221—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance by investigating the dielectric properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/02—Analysing fluids
    • G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/025—Change of phase or condition
    • G01N2291/0256—Adsorption, desorption, surface mass change, e.g. on biosensors
    • G01N2291/0257—Adsorption, desorption, surface mass change, e.g. on biosensors with a layer containing at least one organic compound

Abstract

Die vorliegende Erfindung betrifft eine Vorrichtung, mit der die Hybridisierung von Oligo- und/oder Polynukleotiden nachgewiesen werden kann. Darüber hinaus betrifft die Erfindung eine Anordnung, mit der verschiedene Oligo- und/oder Polynukleotide mittels elektrochemischer Messungen nachgewiesen werden können.
PCT/DE1999/000460 1998-02-20 1999-02-19 Vorrichtung zur detektion von oligo- und/oder polynukleotid-hybridisierungen WO1999042827A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99915484A EP1055004A2 (de) 1998-02-20 1999-02-19 Vorrichtung zur detektion von oligo- und/oder polynukleotid-hybridisierungen

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19807339.9 1998-02-20
DE19807338A DE19807338A1 (de) 1998-02-20 1998-02-20 Kapazitive Vorrichtung für die Detektion der Polynukleotid-Hybridisierung
DE19807338.0 1998-02-20
DE1998107339 DE19807339A1 (de) 1998-02-20 1998-02-20 Sensitive Oberfläche für Biosensor - Systeme

Publications (2)

Publication Number Publication Date
WO1999042827A2 WO1999042827A2 (de) 1999-08-26
WO1999042827A3 true WO1999042827A3 (de) 1999-10-28

Family

ID=26044047

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/000460 WO1999042827A2 (de) 1998-02-20 1999-02-19 Vorrichtung zur detektion von oligo- und/oder polynukleotid-hybridisierungen

Country Status (2)

Country Link
EP (1) EP1055004A2 (de)
WO (1) WO1999042827A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE335201T1 (de) 1999-05-14 2006-08-15 Iris Biotechnologies Inc Reversibel immobilisierung von liganden an metalloberflächen, deren herstellung und verwendung als biosensoren
WO2001001144A2 (en) * 1999-06-30 2001-01-04 Iris Bio Technologies Hybridization of target dna with immobilized nucleic acid analogs
DE10113355A1 (de) * 2000-04-28 2001-09-13 Vladimir M Mirsky Kalibrierung und Erhöhung der Selektivität der Chemosensoren
DE10048997A1 (de) * 2000-09-27 2002-04-11 Santron Ag Vorrichtung zum Nachweis geladener Makromoleküle
AT413214B (de) * 2003-01-24 2005-12-15 Sy Lab Vgmbh Messanordnung und verfahren zur detektion einer dna-sequenz

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993020230A1 (en) * 1992-03-27 1993-10-14 Environmental & Medical Products Ltd Electrochemical detection of dna hybridisation
US5653939A (en) * 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
WO1999014596A1 (en) * 1997-09-15 1999-03-25 Ab Sangtec Medical Capacity affinity sensor
WO1999027355A1 (de) * 1997-11-21 1999-06-03 Wolfbeis, Otto, Samuel Verfahren zur bildung lateral organisierter strukturen auf trägeroberflächen
WO1999028047A2 (de) * 1997-11-29 1999-06-10 Wolfbeis, Otto, Samuel Künstliche oberfläche mit vorbestimmter affinität
DE19901761A1 (de) * 1999-01-18 1999-07-01 Gerhard Dr Hartwich Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5653939A (en) * 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
WO1993020230A1 (en) * 1992-03-27 1993-10-14 Environmental & Medical Products Ltd Electrochemical detection of dna hybridisation
WO1999014596A1 (en) * 1997-09-15 1999-03-25 Ab Sangtec Medical Capacity affinity sensor
WO1999027355A1 (de) * 1997-11-21 1999-06-03 Wolfbeis, Otto, Samuel Verfahren zur bildung lateral organisierter strukturen auf trägeroberflächen
WO1999028047A2 (de) * 1997-11-29 1999-06-10 Wolfbeis, Otto, Samuel Künstliche oberfläche mit vorbestimmter affinität
DE19901761A1 (de) * 1999-01-18 1999-07-01 Gerhard Dr Hartwich Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
HASHIMOTO K ET AL.: "Novel DNA sensor for electrochemical gene detection", ANALYTICA CHIMICA ACTA, vol. 286, 1994, pages 219 - 224, XP002113742 *
MARRAZZA G ET AL.: "Disposable DNA electrochemical sensor for hybridization detection", BIOSENSORS & BIOELECTRONICS, vol. 14, 1999, pages 43 - 51, XP002113746 *
MIKKELSEN S R: "Electrochemical sensors for DNA sequence detection", ELECTROANALYSIS, vol. 8, no. 1, 1996, pages 15 - 19, XP002113745 *
SCHLENOFF J B ET AL.: "Stability and self-exchange in alkanethiol monolayers", THE JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 117, 1995, pages 12528 - 12536, XP002113743 *
TARLOV M J: "Electron-transfer reaction of cytochrome c adsorbed on carboxylic acid terminated alkanethiol monolayer electrodes", THE JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 113, 1991, pages 1847 - 1849, XP002113744 *
WANG J ET AL.: "Nucleic-acid immobilization, recognition and detection at chronopotentiometric DNA chips", BIOSENSORS & BIOELECTRONICS, vol. 12, no. 7, 1997, pages 587 - 599, XP002113741 *
WANG J ET AL: "DNA ELECTROCHEMICAL BIOSENSOR FOR THE DETECTION OF SHORT DNA SEQUENCES RELATED TO THE HUMAN IMMUNODEFICIENCY VIRUS", ANALYTICAL CHEMISTRY, vol. 68, no. 15, 1 August 1996 (1996-08-01), pages 2629 - 2634, XP000623884, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
EP1055004A2 (de) 2000-11-29
WO1999042827A2 (de) 1999-08-26

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