WO1998035755A1 - Mikrokammerarray mit hoher kammerdichte und verfahren zu seiner herstellung - Google Patents
Mikrokammerarray mit hoher kammerdichte und verfahren zu seiner herstellung Download PDFInfo
- Publication number
- WO1998035755A1 WO1998035755A1 PCT/EP1998/000835 EP9800835W WO9835755A1 WO 1998035755 A1 WO1998035755 A1 WO 1998035755A1 EP 9800835 W EP9800835 W EP 9800835W WO 9835755 A1 WO9835755 A1 WO 9835755A1
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- WIPO (PCT)
- Prior art keywords
- chamber
- recesses
- micro
- functional surface
- areas
- Prior art date
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
- B01J2219/00315—Microtiter plates
- B01J2219/00317—Microwell devices, i.e. having large numbers of wells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00511—Walls of reactor vessels
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00617—Delimitation of the attachment areas by chemical means
- B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00614—Delimitation of the attachment areas
- B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
Definitions
- the invention relates to a Mikrosch ⁇ ay with a high chamber density.
- Arrays of MiloOhuntn with single-chamber voluinina in the submicron area are used in biotechnology, drug screening and combinatorial chemistry for parallel and automated handling of small amounts of liquid.
- Arrays with cavities and methods for their production are known which are based on synthetic resins (JP 2-95258; JP 2-35360) or on the basis of hydrophobic polymers which have been subjected to selective plasma treatment (US Pat. No. 5,041,266; US -PS 5,229,163; U.S. Patent 4,741,619).
- the chamber recesses are produced by simple mechanical drilling in a Teflon substrate.
- the main disadvantages of this group of chamber arrays are that, with regard to the present invention, only known recesses can be produced with relatively large distances using techniques known today, and that these chamber arrays are not autoclavable and are therefore disposable.
- micromechanical structures and cavities in semiconducting material or glass on the basis of wet chemical etching techniques (DE 39 15 920 AI) or dry etching techniques in conjunction with photohthographic slides (US Pat. No. 5,462,839) is also described.
- G. Mayer, JM Köhler in "Microchemical Compartments for Biotechnological Applications: Fabrication and Investigation of Liquid Evaporation, Proc. Of Eurosensors X, Vol 2, Leuven, Belgium, Sept. 1996” describe micro-chambers in the submicroliter range, which are made from silicon wafers, in a large number of recesses are made by means of suitable masking processes and anisotropic wet chemical etching techniques.
- these chambers are filled with liquids, in particular aqueous solutions, infested with micropipettes.
- liquids in particular aqueous solutions
- micropipettes With the last described group of microarrays there is a risk that the individual chamber contents can be crosstalk into neighboring chambers. Since, due to the small chamber volumes and a consequent noticeable loss of evaporation, efforts are made to work in a saturated vapor atmosphere, the probability of cross-talk increases, for example, through water vapor condensation in areas between the chambers. In order to largely suppress this risk of possible mixing of chamber contents or contaminations, according to the known prior art, the only option left is to design the chambers at a sufficient distance from one another, which reduces the number of chambers per given area.
- the invention has for its object to provide an M-Lkroscharray with the highest possible chamber density per area and a method for their production in which crosstalk of neighboring chamber contents is prevented even when chamber filling, without the type of chamber filling requiring a change.
- the essence of the invention is that in a carrier provided with a plurality of recesses, which is to be used as an M-micro-chamber array, the carrier surface regions which separate the individual chamber-like recesses on the open chamber side are provided with a functional group, the Effect is against the surface coverage of the chamber inner wall areas. If aqueous solutions are used, the carrier surface areas mentioned are given a hydrophobic property, whereas the
- Chamber inner wall areas should have a hydrophilic property.
- the awarding of the desired surface coverage of the named carrier surface areas is within the scope of the invention achieved in that these areas are contacted at least once by an unprofiled elastomeric body providing or causing a functional surface covering.
- hydrophilic surface coatings such as OH groups rich in sulfonic acid groups or surfaces rich in carboxyl groups
- the second functional surface coating which can be achieved with the aid of a elastomeric bodies are to be generated and to prevent crosstalk between the chambers to be achieved by alkoxy groups, fluoralkane groups, fluoraryl groups, oxyaryl groups, alkoxysilanes or alkyl- or arylsiloxanes.
- FIG. 1 shows, by way of example, a micro-chamber array
- FIG. 2 shows a partial sectional plane of the micro-chamber array according to FIG. 1 with a guide along a line AA
- FIG. 3 shows a special configuration of the surface of the micro-chamber array in preparation for producing a hydrophobic surface covering
- FIGS. 3a and 3b each have a variant of an embodiment according to FIG. 3,
- Fig. 4 indicated the generation of a hydrophobic
- FIG. 1 shows a micro-chamber array consisting of a carrier 2, which in the example is to be made of silicon and can be part of a conventional silicon wafer, which has a plurality of chamber-like recesses 1.
- the recesses have dimensions on the order of 800 ⁇ m • 800 ⁇ m with a volume of 140 nl and the micro-chamber array in this case has over one hundred such recesses per cm 2 .
- Micro-chamber arrays with chamber volumes of the order of 20 nl can also be produced, in which case 400 such recesses / cm 2 can be reached.
- FIG. 2 shows a partial sectional plane of the micro-chamber array according to FIG. 1 with a cut along a line AA.
- the micro-chamber array is first coated over the entire surface with a 50 to 100 nm thick gold layer 31 using conventional sputtering methods, only the gold coating of the surfaces 3 of the web regions being shown in FIG. 3.
- a further silicon wafer 40 is provided with an elastomeric coating 41, which is advantageously formed by spin-on polydimethylsiloxane, which is hardened after spinning.
- the non-profiled silicone stamp 4 activated in this way is placed on the surface of the micro-chamber array, as indicated in FIG. 4 by a double arrow.
- This causes the formation of a chemically very stable gold-thiol bond on the surface 3 of the webs spacing the open individual chambers 1, after which the stamp 4 is removed.
- the monomolecular layer 32 of a thioalkane formed on the regions 3 of the Mi-chamber chamber only modifies these regions, while the chamber inner walls 10 remain unaffected, in the present example as pure gold surfaces, not shown.
- the thin gold layer remaining on the inner walls of the chamber can be removed by selective etching without the surface regions 32 modified with the thioalkane layer being attacked (cf. FIG.
- a 0.1 M KCN solution with 0.001 MK 3 Fe (CN) oil as an oxidizing agent is suitable as an etchant for this.
- the etching time for a 50 nm thick gold layer is less than 1 min.
- the subsequent further step just described can, however, also be carried out in such a way that the on the chamber inner walls remaining gold layer 31 is not removed selectively, but is modified by a further selective reaction with a further miofunctional group, for example thioglycerol.
- the thioglycerin binds to the bare gold-coated inner chamber walls to form a layer 12, but not to the gold-coated surface areas 32 modified with thioalkane, which is indicated in a partial section in FIG. 3b. This also imparts hydrophilic properties to the inner chamber walls and improves the wetting behavior compared to the hydrophobic outer areas even further.
- the thioalkane layer formed has a highly hydrophobic effect, whereas the surfaces 10 of the inner walls of the chamber have a hydrophilic behavior.
- a drop-shaped overhang 5 that forms assumes an edge angle ⁇ of> 90 ° without the liquid crosstalking between the adjacent individual chambers .
- a contact angle ⁇ of> 30 ° is established in the example according to FIG. 5b.
- the invention is not restricted to the exemplary embodiment shown. Particularly when using non-aqueous solutions, such as in ethanol, toluene or acetonitrile, it is important within the scope of the invention to design the wetting behavior of said surface areas 3, significantly different from the wetting behavior of the chamber inner wall areas, which is done by a suitable selection of different first and second functional groups, where non-wetting substances are suitable for the first functional groups, such as alkanes and silanes, and wetting substances are suitable for the second functional groups, such as glycerol, alcohols, carboxylic acids or esters, and the application of the second functional group by means of mentioned elastomeric stamp 4 succeeds.
- first functional groups such as alkanes and silanes
- wetting substances are suitable for the second functional groups, such as glycerol, alcohols, carboxylic acids or esters
- the exemplary design of the elastomeric stamp as a flat, Unprofiled plate was not a limitation of the invention only to this. It would also be possible to use a plate curved in one dimension or a roller-shaped body which carries the second functional group and detects the desired surface areas 3 of the micro-chamber array by unrolling.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98910664A EP1089821B1 (de) | 1997-02-15 | 1998-02-13 | Mikrokammerarray mit hoher kammerdichte und verfahren zu seiner herstellung |
JP53536398A JP2001513695A (ja) | 1997-02-15 | 1998-02-13 | 高密度の微小コンパートメントアレイおよびその製造方法 |
AT98910664T ATE339876T1 (de) | 1997-02-15 | 1998-02-13 | Mikrokammerarray mit hoher kammerdichte und verfahren zu seiner herstellung |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19705910A DE19705910C1 (de) | 1997-02-15 | 1997-02-15 | Mikrokammerarray mit hoher Kammerdichte |
DE19705910.4 | 1997-02-15 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09355442 A-371-Of-International | 1999-07-28 | ||
US10/631,445 Division US20040022692A1 (en) | 1997-02-15 | 2003-07-31 | Microcompartment array with high compartment density and process for producing the same |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998035755A1 true WO1998035755A1 (de) | 1998-08-20 |
Family
ID=7820405
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP1998/000835 WO1998035755A1 (de) | 1997-02-15 | 1998-02-13 | Mikrokammerarray mit hoher kammerdichte und verfahren zu seiner herstellung |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1089821B1 (de) |
JP (1) | JP2001513695A (de) |
AT (1) | ATE339876T1 (de) |
DE (1) | DE19705910C1 (de) |
WO (1) | WO1998035755A1 (de) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6764652B2 (en) | 2001-01-24 | 2004-07-20 | The Regents Of The University Of Michigan | Micromachined device for receiving and retaining at least one liquid droplet, method of making the device and method of using the device |
AU2002361220A1 (en) * | 2001-12-31 | 2003-07-15 | Institut Fur Physikalische Hochtechnologie E.V. | Micro-recess array for size-dependent sorting or separation of cells suspended in a flowing medium and method for analysing the functional activity of individual cells |
WO2003056330A2 (de) * | 2001-12-31 | 2003-07-10 | Institut für Physikalische Hochtechnologie e.V. | Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen |
FR2836072B1 (fr) * | 2002-02-21 | 2004-11-12 | Commissariat Energie Atomique | Composant utilisant un materiau composite et destine a un microsysteme d'analyse biologique ou biochimique |
DE10207616A1 (de) * | 2002-02-22 | 2003-09-04 | Sunyx Surface Nanotechnologies | Ultraphober Probenträger mit funktionalen hydrophilen und/oder oleophilen Bereichen |
US8124423B2 (en) | 2003-09-30 | 2012-02-28 | Alcatel Lucent | Method and apparatus for controlling the flow resistance of a fluid on nanostructured or microstructured surfaces |
KR100544860B1 (ko) * | 2005-02-07 | 2006-01-24 | (주)프로테오니크 | 시료 플레이트 및 이의 제조방법 |
US7619215B2 (en) | 2005-02-07 | 2009-11-17 | Yangsun Kim | Sample plate for MALDI mass spectrometry and process for manufacture of the same |
US7666665B2 (en) | 2005-08-31 | 2010-02-23 | Alcatel-Lucent Usa Inc. | Low adsorption surface |
US8734003B2 (en) | 2005-09-15 | 2014-05-27 | Alcatel Lucent | Micro-chemical mixing |
US8721161B2 (en) | 2005-09-15 | 2014-05-13 | Alcatel Lucent | Fluid oscillations on structured surfaces |
US7412938B2 (en) | 2005-09-15 | 2008-08-19 | Lucent Technologies Inc. | Structured surfaces with controlled flow resistance |
JP7350632B2 (ja) * | 2019-11-18 | 2023-09-26 | 株式会社住化分析センター | 分析方法、および分析用キット |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH0235360A (ja) * | 1988-07-25 | 1990-02-05 | Toppan Printing Co Ltd | マイクロプレートの製造方法 |
JPH0295258A (ja) * | 1988-09-30 | 1990-04-06 | Toppan Printing Co Ltd | マイクロプレートの製造方法 |
WO1993009668A1 (en) * | 1991-11-22 | 1993-05-27 | Affymax Technology N.V. | Combinatorial strategies for polymer synthesis |
US5462839A (en) * | 1993-05-24 | 1995-10-31 | Universite De Neuchatel | Process for the manufacture of a micromachined device to contain or convey a fluid |
BE1009875A3 (fr) * | 1995-12-20 | 1997-10-07 | Yvo Boterdael | Lamelle destinee a des tests pour diagnostics et procedes pour sa fabrication. |
Family Cites Families (5)
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US4741619A (en) * | 1987-05-05 | 1988-05-03 | Molecular Devices Corporation | Hydrophilic microplates for vertical beam photometry |
DE3915920A1 (de) * | 1989-05-16 | 1990-11-22 | Messerschmitt Boelkow Blohm | Mikromechanische struktur |
US5229163A (en) * | 1989-12-21 | 1993-07-20 | Hoffmann-La Roche Inc. | Process for preparing a microtiter tray for immunometric determinations |
US5041266A (en) * | 1989-12-21 | 1991-08-20 | Hoffmann-La Roche Inc. | Tray for immunometric determinations |
DE4209064C2 (de) * | 1992-03-20 | 1995-07-27 | Gerhard Dr Noss | Verfahren zum Zuführen von Waschflüssigkeit zu kleinen, transparenten, mit Zellkulturen beschichteten Testfeldern und zum Absaugen der Waschflüssigkeit von den Testfeldern |
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1997
- 1997-02-15 DE DE19705910A patent/DE19705910C1/de not_active Expired - Fee Related
-
1998
- 1998-02-13 JP JP53536398A patent/JP2001513695A/ja active Pending
- 1998-02-13 AT AT98910664T patent/ATE339876T1/de not_active IP Right Cessation
- 1998-02-13 WO PCT/EP1998/000835 patent/WO1998035755A1/de active IP Right Grant
- 1998-02-13 EP EP98910664A patent/EP1089821B1/de not_active Expired - Lifetime
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0235360A (ja) * | 1988-07-25 | 1990-02-05 | Toppan Printing Co Ltd | マイクロプレートの製造方法 |
JPH0295258A (ja) * | 1988-09-30 | 1990-04-06 | Toppan Printing Co Ltd | マイクロプレートの製造方法 |
WO1993009668A1 (en) * | 1991-11-22 | 1993-05-27 | Affymax Technology N.V. | Combinatorial strategies for polymer synthesis |
US5462839A (en) * | 1993-05-24 | 1995-10-31 | Universite De Neuchatel | Process for the manufacture of a micromachined device to contain or convey a fluid |
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JP2001513695A (ja) | 2001-09-04 |
ATE339876T1 (de) | 2002-11-15 |
EP1089821B1 (de) | 2002-10-23 |
DE19705910C1 (de) | 1998-06-18 |
EP1089821A1 (de) | 2001-04-11 |
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