WO1995013090A1 - Improved interferon polymer conjugates - Google Patents

Improved interferon polymer conjugates Download PDF

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Publication number
WO1995013090A1
WO1995013090A1 PCT/US1994/013207 US9413207W WO9513090A1 WO 1995013090 A1 WO1995013090 A1 WO 1995013090A1 US 9413207 W US9413207 W US 9413207W WO 9513090 A1 WO9513090 A1 WO 9513090A1
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Prior art keywords
interferon
alpha
conjugate
polymer
surfactant
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PCT/US1994/013207
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English (en)
French (fr)
Inventor
Carl W. Gilbert
Myung-Ok Cho
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Enzon, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzon, Inc. filed Critical Enzon, Inc.
Priority to NZ276943A priority Critical patent/NZ276943A/en
Priority to DE69430251T priority patent/DE69430251T2/de
Priority to EP95902571A priority patent/EP0730470B1/en
Priority to AU11798/95A priority patent/AU691225B2/en
Priority to CA002176229A priority patent/CA2176229C/en
Priority to DK95902571T priority patent/DK0730470T3/da
Priority to JP7514074A priority patent/JPH09506087A/ja
Priority to KR1019960702425A priority patent/KR960705579A/ko
Priority to AT95902571T priority patent/ATE214940T1/de
Publication of WO1995013090A1 publication Critical patent/WO1995013090A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/08—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using activating agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention is directed to long-acting interferon-containing preparations.
  • Conjugating biologically-active proteins to polymers has been suggested to improve one or more of the properties of circulating life, water solubility or antigenicity in vivo.
  • PEG polyethylene glycol
  • Insulin and hemoglobin were among the first therapeutic agents conjugated. These relatively large polypeptides contain several free e-amino attachment sites. Several polymers could be attached without significant loss of biologic activity.
  • Interferons are a particular example of proteins which could benefit from improved polymer conjugation techniques. See, for example, U.S. Patent Nos. 4,766,106 and 4,917,888 which describe inter alia beta interferon conjugated with methoxypolyethylene glycol N-succinimidyl glutarate or methoxypolyethylene glycol N-succinimidyl succinate. The conjugation reactions were carried out using relatively high molar excesses (10, 20 and 50-fold) of the polymer. European Patent Application bearing publication No. 0
  • the present invention addresses these shortcomings.
  • One aspect of the invention provides a process for preparing long-acting alpha-interferon-containing compositions.
  • the process includes contacting alpha- interferon with a substantially non-antigenic polymer in the presence of a surfactant under conditions which are sufficient to effect conjugation of the protein and polymer.
  • Suitable alpha-interferons include recombinant and alpha-interferons isolated from mammals.
  • the polymer portion of the conjugate is preferably a polyalkylene oxide (PAO) , such as mono-alkyl terminated
  • PAO's like mono-methyl polyethylene glycol ( PEG) PAO's like mono-methyl polyethylene glycol ( PEG) .
  • the non-alkyl-terminated end of the polymer is functionalized with a reactive group capable of bonding with the alpha- interferon, preferably at the epsilon (e) amino acid lysines.
  • the polymers may have a molecular weight of from about 200 to about 35,000. Other substantially non- antigenic polymers can also be used.
  • the conditions for effecting conjugation include conducting the reaction with a relatively small molar excess of the polymer with respect to the alpha-interferon.
  • the range of molar excess for the polymer can be from about 1 to 8-fold; molar excesses of from about 1.5 to 7 are preferred and a range of from about 1.75 - 5-fold are particularly preferred.
  • the conditions further include contacting the reactants in the presence of a surfactant which is present in amounts ranging from about 0.1 to about 1% by weight.
  • a surfactant which is present in amounts ranging from about 0.1 to about 1% by weight.
  • One particularly preferred ionic surfactant is sodium dodecyl sulfate (SDS) .
  • SDS sodium dodecyl sulfate
  • Other ionic and non-ionic surfactants can also be used.
  • the resulting conjugates can have from about 0 to about 6 polymeric strands attached to each alpha-interferon molecule. After conjugation, those species containing about 1-4 polymer strands per IFN and most preferably those conjugates containing about 2 polymer strands per IFN molecule are fractionated away from the other species.
  • the invention also includes methods of treating alpha- interferon susceptible conditions in mammals. In this aspect, treatment includes administering an effective amount of the conjugates described herein to mammals requiring such therapy.
  • highly active, long lasting alpha-interferon-containing conjugates are provided.
  • the isolated species provide predictable, uniform activity.
  • the alpha-interferon ( ⁇ .-IFN) portion of the polymer conjugate can be prepared or obtained from a variety of sources including recombinant techniques such as those using synthetic genes expressed in E. coli. See also Pestka, "Interferon ⁇ .” in Human Cytokines. Blackwell Scientific Publications 1-16 (1992) , the disclosure of which is incorporated herein by reference.
  • the Q.IFN can also be a mammalian extract such as human, ruminant or bovine ⁇ lNF.
  • ⁇ _IFN is INFof-2b, a recombinantly-made product of the Schering Corp., Kenilworth, NJ. Alternate embodiments, where the foreign O.INF is not completely autologous, may be used since the polymeric modification sufficiently reduces antigenic responses.
  • ⁇ -IFN effect in mammals means any substance which demonstrates in vivo activity corresponding to that observed with c-IFN's. These substances are prepared by using techniques known to those of ordinary skill in the art such as tissue culture, extraction from animal sources or by recombinant DNA methodologies. Transgenic sources of O.IFN and related moieties are also contemplated. Such materials are obtained from transgenic animals, i.e. mice, pigs, cows, etc.
  • ⁇ _IFN has certain advantages over other interferon species such as ⁇ and ⁇ IFNs.
  • the ⁇ .IFN's are preferred because of their biochemical and serological properties.
  • c_IFN has documented antiviral properties and diffuses more effectively into the bloodstream than other interferons.
  • ⁇ lFN has three lysines in active site area of the polypeptide. It has been surprisingly determined that the attachment techniques described herein sufficiently protect these lysines from polymeric attachment (and inactivation) during conjugation. As will be discussed below, most polymers attach at ⁇ lFN lysines which are not associated with bioactivity.
  • one of the polymer hydroxyl end- groups is converted into a reactive functional group which allows conjugation. This process is frequently referred to as “activation” and the product is called an "activated poly(alkylene oxide)".
  • Other substantially non-antigenic polymers are similarly "activated” or functionalized.
  • the activated polymers are reacted with ⁇ _IFN so that attachment preferably occurs at e-amino groups of lysines. Free carboxylic acid groups, suitably activated carbonyl groups, oxidized carbohydrate moieties and mercapto groups if available on the IFN can also be used as attachment sites.
  • urethane linkages are formed between with the ⁇ _IFN e amino groups and the activated polyalkylene oxides.
  • the urethane linkage is formed as described in commonly owned U.S. Patent No. 5,122,614, the disclosure of which is hereby incorporated by reference. This patent discloses the formation of N-succinimide carbonate derivatives of polyalkylene oxides. Polymers activated with amide-forming linkers or the like are also contemplated. Other functional groups which facilitate attachment of the polymer to the IFN via e amino or other groups are also contemplated.
  • PAO's mono- activated, alkyl-terminated polyalkylene oxides
  • mPEG's monomethyl-terminated polyethylene glycols
  • bis-activated polyethylene oxides are also contemplated for purposes of cross-linking ⁇ lFN's or providing a means for attaching other moieties such as targeting agents for localizing the polymer-c_IFN conjugate in a particular area such as, for example, the liver.
  • Suitable polymers will vary substantially by weight. Polymers having molecular weights ranging from about 200 to about 35,000 are usually selected for the purposes of the present invention. Molecular weights of from about 1,000 to about 15,500 are preferred and 2000 to about 12,500 are particularly preferred.
  • the polymeric substances included are also preferably water-soluble at room temperature.
  • a non-limiting list of such polymers include polyalkylene oxide homopolymers such as polyethylene glycol (PEG) or polypropylene glycols, polyoxyethylenated polyols, copolymers thereof and block copolymers thereof, provided that the water solubility of the block copolymers maintained.
  • PEG polyethylene glycol
  • C__ 4 alkyl-terminated polymers are also useful.
  • PAO-based polymers effectively non-antigenic materials such as dextran, polyvinyl pyrrolidones, polyacrylamides, polyvinyl alcohols, carbohydrate-based polymers and the like can be used.
  • the surfactants used in the processes of the present invention are ionic-type agents.
  • One particularly preferred agent is sodium dodecyl sulfate,
  • SDS sodium dodecyl sulfate
  • quaternary ammonium compounds taurocholic acid
  • caprylic acid caprylic acid
  • decane sulfonic acid etc.
  • Non-ionic surfactants can also be used.
  • materials such as polyoxyethylene sorbitans (Tweens) , polyoxyethylene ethers (Tritons) can be used. See also Neugebauer, A Guide to the Properties and Uses of Detergents in Biology and Biochemistry (1992) Calbiochem Corp.
  • the only limitations on the type of surfactant used in the processes of the invention are that they do not cause substantial denaturation of the IFN and do not completely inhibit polymer conjugation.
  • the surfactants are present in the reaction mixtures in amounts from about 0.01-0.5 ; preferably from 0.05-0.5 %,- and most preferably from about 0.075-0.25 %. Mixtures of the surfactants are also contemplated.
  • O.IFN contains three lysines in the active site region. This relatively positive-charged area of the polypeptide has been found to undergo substantial polymer conjugation during solution-based processes without some kind of protection. This results in substantial or complete loss of bioactivity.
  • the surfactants have been found to be surprisingly effective in selectively discouraging polymer conjugation in this area while allowing lysine-based conjugation to proceed on other areas of the polypeptide.
  • Conjugation reactions sometimes referred to as PEGylation reactions, are often carried out in solution without regard to where the polymer will attach to the protein. Such techniques however have proven to be inadequate for conjugating o_IFN. As described above, it has been determined that a key to maintaining bioactivity is to substantially avoid including those lysines in the active site in the polymer coupling process.
  • the processes of the present invention achieves this goal by carrying out the conjugation reaction in the presence of a surfactant with relatively small molar excesses of the polymer with respect to the ⁇ lFN.
  • the process is carried out with about 1-8-fold molar excesses; preferably about 1.5-7-fold molar excesses and most preferably about 1.75-5-fold molar excesses.
  • conjugation is effected using small molar excesses of activated polymer without a surf ctant.
  • the process preferably includes combining the ⁇ lFN in solution with the surfactant prior to introducing the activated polymer.
  • the complete conjugation reaction can be carried out at about room temperature, 20-25°C. It is also preferred that the coupling reaction be allowed to proceed for rather short periods of time, i.e. 1-2 hours, before quenching.
  • the inventive process produces conjugates having varying degrees of polyalkylene oxide substitution. Residual unconjugated PAO's and C.IFN can also be present. This mixture is typically in a reaction buffer containing one or more of phosphate, chloride and bicarbonate anions. The PAO, O.IFN and conjugate mixture is preferably fractionated in a buffer solution containing from about 1- 10 mg/ml PAO—c_IFN conjugates. Suitable solutions have a pH of from about 7.0 to about 9.0 and preferably from about 7.5 to about 8.5.
  • the solutions preferably contain one or more buffer salts selected from KC1, NaCl, K 2 HP0 4 , KH 2 P0 4 , Na 2 HP0 4 , NaH 2 P0 4 , NaHC0 3 , NaB0 4 , (NH 4 ) 2 C0 3 and glycine NaOH.
  • buffer salts selected from KC1, NaCl, K 2 HP0 4 , KH 2 P0 4 , Na 2 HP0 4 , NaH 2 P0 4 , NaHC0 3 , NaB0 4 , (NH 4 ) 2 C0 3 and glycine NaOH.
  • Sodium phosphate buffers are preferred.
  • the PAO-O.IFN conjugate solution may first have to undergo buffer exchange/ultrafiltration.
  • the PAO- ⁇ lFN conjugate solution can be ultrafiltered across a low molecular weight cut-off (10,000 to 30,000 dalton) membrane which will also remove most surfactants as well.
  • the fractionation of the conjugates into desired species is preferably carried out using an anion exchange medium.
  • Such media are capable of selectively binding those PAO-O.IFN conjugates having 1-4 PAO strands, excess PAO and unmodified o_IFN.
  • This fractionation occurs since the ⁇ lFN molecules of various degrees of substitution will have isoelectric points which vary in a somewhat predictable fashion.
  • the isoelectric point of O.IFN is determined by the number of available lysine residues available on the surface of the protein. These lysine residues also serve as the point of attachment of polyalkylene oxide conjugates. Therefore, as the degree of substitution of polyalkylene oxide increases, the isoelectric point decreases, and the ability of the conjugate to bind to an anion exchange resin weakens.
  • the use of strongly polar anion exchange resins are especially preferred for the method of the present invention. For this reason, quaternary amine coated anion exchange resins are utilized.
  • the quaternary amine resin may be coated onto either a polymeric or silica matrix; however, polymeric matrices are preferred.
  • a number of tetramethylamine, or quaternary methylamine, anion exchange resins are commercially available, coated onto the support matrices.
  • quaternary anion exchange resins suitable for use with the present invention are Q-HD, QA TRISACRYL ® and QMA—SPHEROSIL ® , quaternary amine resins coated onto a polymer matrix, manufactured by IBF of Garenne, France, for Sepracor of Marlborough, Massachusetts; TMAE650M ® , a tetramethylamino ethyl resin coated onto a polymer matrix, manufactured by EM—Separators of Gibbstown, New Jersey; QAE550C ® , and SUPER C ® , each a quaternary amine resin coated onto a polymer matrix and manufactured by TosoHaas of Montgomeryville, PA.
  • QMA Accell manufactured by Millipore of Millford, MA and PEI resins manufactured by JT Baker of Phillipsburg, NJ, may also be used.
  • the anion exchange resin is packed in the column and equilibrated by conventional means.
  • a buffer having the same pH and osmolality as the conjugated ⁇ _IFN solution is used.
  • the conjugate-containing solution is then adsorbed onto the column.
  • a gradient flow of an elution buffer with increasing salt concentrations is applied to the column to elute the desired fractions of polyalkylene oxide-conjugated CUIFN.
  • the fractions are of essentially uniform molecular weight and degree of substitution.
  • Preferred polyalkylene oxide conjugate fractions have 1-4 polyalkylene oxide strands per O.IFN molecule. Preferably the fraction contains about 1-3 and most preferable about 2 PAO strands per c-IFN molecule.
  • the elution buffer preferably contains one or more salts selected from KC1, NaCl, K 2 HP0 4 , KH 2 P0 4 , Na 2 HP0 4 , NaH 2 P0 4 , NaHC0 3 , NaB0 4 and (NH 4 ) 2 C0 3 . These fractions are substantially free of other conjugates. Any unconjugated species can then be backwashed from the column by conventional techniques.
  • the sample is washed with 10 mM NaP0 4 to remove any unreacted PAO and thereafter a step gradient elution with NaCl is used. Elution with 10 mM NaCl recovers fractions containing conjugates with greater than 3 polymer strands PAO per IFN; elution with 50 mM NaCl recovers conjugates containing 1- 2 strands; elution with 150 mM NaCl recovers unmodified IFN.
  • Another aspect of the present invention provides methods of treatment for various medical conditions in mammals.
  • the methods include administering an effective amount of Q_IFN-polymer conjugate which has been prepared as described herein to a mammal in need of such treatment.
  • the conjugates are useful for, among other things, treating interferon-susceptible conditions or conditions which would respond positively or favorably as these terms are known in the medical arts to interferon-based therapy.
  • the interferon conjugates can be used to treat conditions which would benefit from the inhibiting replication of interferon-sensitive viruses.
  • PEG S000 fraction The PEG- ⁇ lFN in sodium phosphate buffer was loaded onto a QHD anion exchange column. The 2-PEG fraction was eluted with a gradient from 0 to 400 mm sodium chloride in 10 Mm phosphate Ph 8. The 2-PEG fraction was verified with using size exclusion chromatography and SDS-
  • Example 3 the product of Example 3, (SDS-2-PEG- 5000 r ⁇ _IFN) , 2-PEG 5000 r ⁇ .IFN made in the absence of a surfactant and unconjugated r ⁇ .IFN were tested.
  • Activity was determined using a CPE assay with EMC virus challenging A549 human lung carcinoma cells. Circulating life was determined using an average value obtained from the blood of 3 rats in a group receiving 1 million units, with time points taken over 7 days.

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PCT/US1994/013207 1993-11-10 1994-11-10 Improved interferon polymer conjugates WO1995013090A1 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
NZ276943A NZ276943A (en) 1993-11-10 1994-11-10 Alpha-interferon conjugated to a non-antigenic polymer (preferably a polyalkylene oxide) and its preparation
DE69430251T DE69430251T2 (de) 1993-11-10 1994-11-10 Verbesserte interferon-polymerkonjugate
EP95902571A EP0730470B1 (en) 1993-11-10 1994-11-10 Improved interferon polymer conjugates
AU11798/95A AU691225B2 (en) 1993-11-10 1994-11-10 Improved interferon polymer conjugates
CA002176229A CA2176229C (en) 1993-11-10 1994-11-10 Improved interferon polymer conjugates
DK95902571T DK0730470T3 (da) 1993-11-10 1994-11-10 Forbedrede interferonpolymerkonjugater
JP7514074A JPH09506087A (ja) 1993-11-10 1994-11-10 改良されたインターフェロン−ポリマー複合体
KR1019960702425A KR960705579A (ko) 1993-11-10 1994-11-10 개선된 인터페론 중합체 결합체(Improved interferon polymer conjugates)
AT95902571T ATE214940T1 (de) 1993-11-10 1994-11-10 Verbesserte interferon-polymerkonjugate

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US15064393A 1993-11-10 1993-11-10
US08/150,643 1993-11-10

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JP (1) JPH09506087A (pt)
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AT (1) ATE214940T1 (pt)
AU (1) AU691225B2 (pt)
CA (1) CA2176229C (pt)
DE (1) DE69430251T2 (pt)
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ES (1) ES2174915T3 (pt)
HU (1) HUT75533A (pt)
NZ (1) NZ276943A (pt)
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Cited By (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996007420A1 (en) * 1994-09-03 1996-03-14 The University Of Nottingham Macrophage stimulating composition comprising a non-ionic surfactant
WO1997016204A1 (en) 1995-11-02 1997-05-09 Schering Corporation Continuous low-dose cytokine infusion therapy
EP0809996A2 (en) * 1996-05-31 1997-12-03 F. Hoffmann-La Roche Ag Interferon conjugates
EP0862455A1 (en) * 1995-11-21 1998-09-09 Enzon, Inc. Interferon-polymer conjugates and process for preparing the same
WO1998048840A1 (en) * 1997-04-29 1998-11-05 Schering Corporation Polyethylene glycol-interferon alpha conjugates for therapy of infection
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DK0730470T3 (da) 2002-06-03
EP0730470A4 (en) 1999-11-24
DE69430251T2 (de) 2002-11-07
AU691225B2 (en) 1998-05-14
US5711944A (en) 1998-01-27
AU1179895A (en) 1995-05-29
HUT75533A (en) 1997-05-28
DE69430251D1 (de) 2002-05-02
EP0730470A1 (en) 1996-09-11
ATE214940T1 (de) 2002-04-15
EP0730470B1 (en) 2002-03-27
PT730470E (pt) 2002-08-30
JPH09506087A (ja) 1997-06-17
KR960705579A (ko) 1996-11-08
CA2176229C (en) 2003-05-27
NZ276943A (en) 1998-02-26
CA2176229A1 (en) 1995-05-18
HU9601247D0 (en) 1996-07-29
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