WO1995011441A1 - Luminometre - Google Patents

Luminometre Download PDF

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Publication number
WO1995011441A1
WO1995011441A1 PCT/AT1994/000149 AT9400149W WO9511441A1 WO 1995011441 A1 WO1995011441 A1 WO 1995011441A1 AT 9400149 W AT9400149 W AT 9400149W WO 9511441 A1 WO9511441 A1 WO 9511441A1
Authority
WO
WIPO (PCT)
Prior art keywords
samples
luminometer
radiation
sample
measurement
Prior art date
Application number
PCT/AT1994/000149
Other languages
German (de)
English (en)
Other versions
WO1995011441B1 (fr
Inventor
Richard Landlinger
Original Assignee
Anthos Labtec Instruments Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anthos Labtec Instruments Gmbh filed Critical Anthos Labtec Instruments Gmbh
Priority to AU78025/94A priority Critical patent/AU7802594A/en
Publication of WO1995011441A1 publication Critical patent/WO1995011441A1/fr
Publication of WO1995011441B1 publication Critical patent/WO1995011441B1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus

Definitions

  • the invention relates to a luminometer which, for successive measurement of the radiation emanating from different samples, has a bidirectionally working program-controlled transport device for plate-shaped sample containers which are provided with a plurality of recesses for receiving the samples, with which transport device the samples can be positioned successively at a measuring point provided in the luminometer, at which the luminescence radiation is detected with a radiation detector, characterized in that in the luminometer there is a measuring point provided for photometric measurement on the samples and one for the supply of light to the light source provided at each measuring point and that a bidirectional transport device is provided in the luminometer, which is used both for the positioning of the samples for luminescence measurement and for the positioning of the samples f is provided for a photometric measurement.
  • Luminometers of the aforementioned type are used for many examinations in which larger numbers of samples have to be examined for the presence of certain characteristics, luminescent radiation occurring as an indicator for these characteristics.
  • Such examination methods generally have a high detection sensitivity for the presence of certain characteristics in the samples, and such examination methods are widely used especially in the field of medicine and in the field of biology.
  • ELISA examination methods in which chemiluminescence radiation occurs as an indicator for the presence of certain characteristics in the samples to be examined, are widely used, and there are also examination systems designed for bioluminescence radiation as an indicator Example of applications in the field of genetic testing.
  • plate-shaped sample containers which are provided with a plurality of grid-like recesses for receiving the samples.
  • Such plate-like sample containers are often referred to as microplates or microtiter plates and are provided, for example, with 60, 72 or 96 recesses arranged in the manner of a rectangular grid, into which the samples to be examined are filled.
  • Such depressions can be cylindrical, for example, or can also taper downward, for example in the form of a cone stocking.
  • the program-controlled transport device provided in the luminometer makes it possible, in rapid succession, to position the individual wells of such a plate-shaped sample container precisely at a measuring point of the luminometer at which the luminescence radiation emanating from the individual samples is detected with a radiation detector, and in this way the desired ones Conduct examinations in an automatic manner.
  • examinations of the aforementioned type there is often the need or the need to confirm or supplement the examination result obtained by the luminescence radiation measurement, to carry out a further examination of the samples in question by means of an examination process in which photometrically determined measured values are used as a criterion for the presence of the same characteristics. which have been recorded in the luminescence radiation measurement, or which serve other, additional characteristics.
  • samples of the substances in question have so far been examined in a separate photometric measuring device, and for this purpose either the samples provided for the luminescence radiation examination before the luminescence radiation measurement or after this have been transferred to a separate device for the photometric measurement or, in parallel, a number of identical samples of the substances to be examined are subjected to a luminescence radiation examination on the one hand and a photometric examination on the other.
  • both methods of operation also require a not insignificant amount of manipulation, since in one case the plate-like sample containers containing the samples successively enter one another two different devices must be used, and in the other case, pairs of sample containers filled in the same way must be produced and subjected to the intended examinations in parallel coordination in two different measuring devices.
  • the invention provides a special design of the luminometer of the type mentioned at the outset, which is characterized in that in the luminometer there is a measuring point provided for photometric measurement on the samples and one for the supply of light to the one located at this measuring point
  • the light source provided for the sample is provided and that a bidirectional transport device is provided in the luminometer, which is provided both for the positioning of the samples for luminescence measurement and for the positioning of the samples for a photometric measurement.
  • the luminescence radiation measurement and the photometric measurement can, if desired, to the extent that this is advantageous for the measurements due to the preparation of the samples, in rapid succession in the automatic process, which is particularly advantageous if the examination in question only has relatively short-term radiation emissions occur, and / or the properties of the sample to be determined in the photometric measurement change rapidly with the passage of time.
  • a preferred embodiment of the luminometer designed according to the invention is characterized in that the luminometer has a radiation detector which is alternatively provided on the one hand for the detection of the luminescent radiation and on the other hand for the detection of the radiation emitted by the respective sample during photometric measurement.
  • a radiation detector which is alternatively provided on the one hand for the detection of the luminescent radiation and on the other hand for the detection of the radiation emitted by the respective sample during photometric measurement.
  • the radiation detector is sensitive to intensity and / or spectrally sensitive to the radiation Operation as a luminescence radiation detector on the one hand and for operation as a photometry detector on the other hand is switchable.
  • a structurally simple and easily adjustable embodiment can be achieved, in which the undesired influence of extraneous light can also be kept low by providing a by for the supply of light to the sample that is subject to the photometric measurement the light source is provided on the underside of the light guide which carries the samples in recesses, is formed in a translucent or transparent plate-like sample container. It is further advantageous both structurally and with regard to the adjustment to be made and the minimization of extraneous light influences if one provides that a spectral band filter is inserted between the light source and the light guide.
  • the spectral filter of a relatively easily accessible point in the device can be arranged and at the same time the measuring point can be kept compact, which is advantageous both in terms of avoiding the influence of extraneous light and in terms of an exactly adjusted light supply to the samples to be examined.
  • a related embodiment of the luminometer which has the advantage that the filter required for the respective examination can be used in a simple manner, this insertion also being brought about automatically by a control program running for the relevant examination, is characterized in that several in the light path between light source and sample alternatively spectral filters to be inserted are provided, which are arranged on a support provided with a motor-driven adjusting device.
  • a spectral filter is provided in the radiation path between the sample and the detector.
  • This measure allows undesired influences of extraneous light, accompanying radiation, background radiation and similar interference effects to be kept low or suppressed, so that a higher measuring sensitivity and a higher measuring accuracy can be achieved.
  • spectral filters can be easily introduced into the radiation path and also exchanged.
  • an embodiment of the device is favorable, which is characterized in that a plurality of spectral filters which are alternatively to be inserted into the radiation path between the sample and the detector are provided, which are arranged on a support provided with a motor-driven adjusting device.
  • the filters can be so-called edge filters or band filters.
  • the filters are mutually coordinated, which are indicated in the path of the light. are arranged, and filters, which are provided in the radiation path between the sample and the detector, are carried out.
  • filters which are provided in the radiation path between the sample and the detector.
  • fluorescence measurements in which the intensity of a fluorescence radiation of another wavelength range produced by the samples under the influence of a radiation supplied from one wavelength range is measured.
  • a filter effect matched to these examinations can also be achieved by appropriate selection of the material from which the sample containers are made.
  • FIG. 1 shows a first embodiment of a luminometer designed according to the invention in plan view
  • FIG. 3 shows another embodiment of such a luminometer in a sectional view corresponding to FIG. 2, and
  • the luminometer 1 shown in FIGS. 1 and 2 is intended for successive measurement of the radiation 3 emanating from different samples 2.
  • the samples 2 are located in a plate-shaped sample container 4 which is provided with a multiplicity of depressions 5 for receiving the samples 2.
  • the depressions 5 can, as shown with solid lines, be cylindrical or have another shape, such as a frustoconical shape, as shown in broken lines in FIG.
  • sample containers are often called microplates or microtitration plates.
  • the sample container 4 is inserted into a carriage 6, which, along with the guides 11, is connected to a controllable motor-driven displacement device 7, which is connected to the carriage 6 via a cable connection 8 with a deflection roller 9, in the x-direction indicated by the double arrow 10 movable back and forth.
  • the carriage 6 and the guides 11, the Shifting device 7 and the deflecting roller 9 are arranged on a carriage 12 which, in turn, by means of a shifting device 13 fixed in the device via a cable connection 14, which leads over a stationary deflecting roller 15, in the y-direction along guide rails 17, indicated by the double arrow 16, on which the carriage 12 is movably mounted, can be moved back and forth.
  • the carriage 6, together with the guides 11, the carriage 12, the guide rails 17 and the displacement devices 7 and 13, forms a bidirectional transport device with which the plate-like sample container 4 can be moved back and forth in the xy direction that each of the recesses 5, which are provided in the sample container 4, can optionally be brought to the measuring point 18 or positioned there.
  • a radiation detector 19 is arranged at the measuring point 18, which is preferably designed in the form of a photomultiplier.
  • the radiation detector 19 is accommodated in a shield 20, which has an entrance window 21 for the radiation coming from the sample 2.
  • the measuring point 18 is provided not only for the luminescence measurement but also for a photometric measurement on the samples 2.
  • a light source 23 is provided for supplying light to the sample 2 located at the measuring point 18, from which a light guide 24 leads to the underside of the sample container 4 at the measuring point 18.
  • the light emerging from the exit end 25 of the light guide 24 penetrates the bottom of this sample container 4, which is made of translucent or transparent material, and reaches the sample 2 located at the measuring point 18, in which it is influenced. Attenuation may occur, which may also be spectrally selective in a characteristic manner, or spectrally shifted radiation may also arise due to fluorescence in the sample 2 in question.
  • a spectral filter 27 preferably a bandpass filter, is expediently provided between the light source 23 and the entry end 26 of the light guide 24.
  • the arrangement of this filter between the light source and the inlet end 26 of the light guide 24 makes it possible to easily accommodate this filter in the device without being subject to the spatial restrictions that are usually present at the measuring point 18.
  • a spectral filter 28 is further provided between the sample 2 located at the measuring point 18 and the radiation detector 19, which spectral filter 28 can be an edge filter or a bandpass filter depending on the measurement requirements in the particular case.
  • the bidirectional transport device of the luminometer which includes the carriage 6, the displacement unit 7, the carriage 12 and the displacement unit 13, is provided both for the positioning of the samples 2 for luminescence measurement and for the positioning of these samples for a photometric measurement.
  • the radiation detector 19 is also provided both for the detection of the luminescent radiation and for the detection of the radiation emanating from the respective sample 2 during the photometric measurement.
  • the light source 23 is only switched on for the photometric measurement.
  • the radiation detector 19 is sensitive to intensity and / or spectral sensitivity for operation as a luminescent radiation detector on the one hand and for operation as a photometry detector on the other hand is switchable.
  • the intensity sensitivity can be switched over by influencing the output signal of the radiation detector in a subsequent amplifier or a subsequent voltage divider, or else by changing the operating voltages, which is particularly advantageous if the radiation detector 19 is designed as a photomultiplier.
  • a switchover with regard to the spectral sensitivity can advantageously be carried out by changing upstream filters 28.
  • the function of the bidirectional transport device in that the samples provided for a luminescence measurement and also the samples provided for a photometric measurement are in correct coordination with the switching on of the light source 23 to be provided for the photometric measurement and the corresponding setting of the operating properties of the radiation detector 19 is carried out by a control unit 22, which handles the control of the displacement devices 7 and 13 following the measurement program in coordination with the switching on of the light source 23 and the switching on of the associated operating conditions for the radiation detector 19.
  • separate radiation detectors are provided for the detection of the luminescence radiation on the one hand and the radiation to be detected during the photometric measurement on the other hand, namely in addition to a radiation detector 19 in the form of a photomultiplier for detecting the luminescence radiation, another radiation detector 29 for photometric measurement.
  • the radiation detector 29 can be designed in the same way as the radiation detector 19 or in another way, for example as a semiconductor radiation detector. There are thus two measuring points in the embodiment of a luminometer shown in FIG.
  • the measuring point 18 for the luminescence measurement and the measuring point 30 for the photometric measurement namely the measuring point 18 for the luminescence measurement and the measuring point 30 for the photometric measurement, and in this case also the one and only bidirectional transport device provided in the luminometer both is provided for the positioning of the samples at the measuring point 18 for luminescence measurement and for the positioning of the samples at the measuring point 30 for a photometric measurement.
  • the shifting devices of the bidirectional transport device are programmed accordingly, and if the samples 2 are arranged appropriately in the recesses 5 of the sample container 4, the luminescent radiation emanating from one sample can be measured at the same time and a photometric measurement can be carried out on another sample.
  • both luminescence measurements and photometric measurements can of course also be carried out successively on individual samples, the fact that the samples are carried by a single transport device to the Measuring points are positioned, a very rapid transfer of the samples to the respectively intended measuring point can be carried out without the need for an additional time-consuming transfer. This is particularly important when detecting briefly occurring luminescence phenomena or also briefly occurring reactions which are manifested in photometrically detectable phenomena.
  • a liquid dispenser 31 can advantageously be provided, with which substances triggering the reaction can be added to the samples 2 immediately before the measurement.
  • spectral filters 28 which can or should alternatively be inserted into the radiation path between the sample and the detector.
  • These spectral filters 28 are arranged on a support 32 in the form of a circular plate, which is provided with an adjusting device 33 which can be driven by a motor. By correspondingly controlling this adjusting device 33, one of the filters 28 can be inserted into the radiation path between the respective sample to be examined and a radiation detector 19 provided for detecting the radiation.
  • a similar device can also be provided for the alternative insertion of radiation filters 27 in the radiation path leading from a light source provided for photometric measurements to the particular sample to be examined.

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Luminomètre destiné à effectuer des mesures successives de rayonnement par luminescence en provenance de divers échantillons (2) et comprenant un dispositif transporteur (6, 11, 12, 17, 7, 13) bidirectionnel, à commande programmée, pour un récipient d'échantillons (4) en forme générale de plaque présentant une pluralité de cavités (5) destinées à recevoir lesdits échantillons (2). Le luminomètre (1) présente un poste de mesure (18) pour le mesurage photométrique des échantillons, ainsi qu'une source lumineuse (23) envoyant un rayon lumineux à chaque échantillon (2) se trouvant à ce poste de mesure. Le dispositif transporteur agencé sur le luminomètre (1) est prévu pour le positionnement des échantillons d'une part pour des mesures de luminescence et d'autre part pour des mesures photométriques.
PCT/AT1994/000149 1993-10-18 1994-10-12 Luminometre WO1995011441A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU78025/94A AU7802594A (en) 1993-10-18 1994-10-12 Luminometer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA2090/93 1993-10-18
AT209093 1993-10-18

Publications (2)

Publication Number Publication Date
WO1995011441A1 true WO1995011441A1 (fr) 1995-04-27
WO1995011441B1 WO1995011441B1 (fr) 1995-06-15

Family

ID=3527540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT1994/000149 WO1995011441A1 (fr) 1993-10-18 1994-10-12 Luminometre

Country Status (2)

Country Link
AU (1) AU7802594A (fr)
WO (1) WO1995011441A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2738344A1 (fr) * 1995-09-04 1997-03-07 Rech Investissements Sfri Soc Luminometre notamment pour analyses medicales
EP2159567A1 (fr) 2004-09-10 2010-03-03 Wallac Oy Instrumentation améliorée et procédé de mesure optique d'échantillons

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3413065A1 (de) * 1983-04-06 1984-10-18 Baker Instruments Corp., Allentown, Pa. Zentrifugalanalysator
EP0194102A2 (fr) * 1985-03-01 1986-09-10 Wayne F. Lisenbee Installation pour la mesure de la luminescence
WO1991004482A1 (fr) * 1989-09-19 1991-04-04 Park Pharmaceuticals Inc. Luminometre
WO1993009440A1 (fr) * 1991-10-31 1993-05-13 Baxter Diagnostics Inc. Traitement de specimen et systemes et procedes d'analyse, par photometrie

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3413065A1 (de) * 1983-04-06 1984-10-18 Baker Instruments Corp., Allentown, Pa. Zentrifugalanalysator
EP0194102A2 (fr) * 1985-03-01 1986-09-10 Wayne F. Lisenbee Installation pour la mesure de la luminescence
WO1991004482A1 (fr) * 1989-09-19 1991-04-04 Park Pharmaceuticals Inc. Luminometre
WO1993009440A1 (fr) * 1991-10-31 1993-05-13 Baxter Diagnostics Inc. Traitement de specimen et systemes et procedes d'analyse, par photometrie

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2738344A1 (fr) * 1995-09-04 1997-03-07 Rech Investissements Sfri Soc Luminometre notamment pour analyses medicales
WO1997009610A1 (fr) * 1995-09-04 1997-03-13 Societe Française De Recherches Et D'investissements (S.F.R.I.) Luminometre notamment pour analyses medicales
US6377342B1 (en) 1995-09-04 2002-04-23 Societe Francaise De Recherches Et D'investissements (Sfri) Luminometer, particularly for medical assays
EP2159567A1 (fr) 2004-09-10 2010-03-03 Wallac Oy Instrumentation améliorée et procédé de mesure optique d'échantillons
US7843568B2 (en) 2004-09-10 2010-11-30 Wallac Oy Enhanced instrumentation and method for optical measurement of samples

Also Published As

Publication number Publication date
AU7802594A (en) 1995-05-08

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