WO1992001043A1 - Hydridomes produisant une immunoglobuline g aviaire specifique - Google Patents
Hydridomes produisant une immunoglobuline g aviaire specifique Download PDFInfo
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- WO1992001043A1 WO1992001043A1 PCT/JP1991/000923 JP9100923W WO9201043A1 WO 1992001043 A1 WO1992001043 A1 WO 1992001043A1 JP 9100923 W JP9100923 W JP 9100923W WO 9201043 A1 WO9201043 A1 WO 9201043A1
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- Prior art keywords
- cell
- cells
- igg
- fused
- avian
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Definitions
- the present invention relates to an established fusion cell having an Ig-expressing ability obtained from a nitrile, a method for producing the same, and an IgG-producing ability obtained by fusing a nitrile spleen cell immunized to the established fusion cell.
- the present invention relates to a fused cell having the above and a method for producing an antibody using the fused cell.
- nitrile immunoglobulin IgG has very low cross-reactivity with mammalian-derived IgG (Hadge, D., et al, Mol. Immunol., 21, 699-707, 1984). It is also known that nitrile IgG does not bind to protein A (Guss, E. etal EMBO J., 5, 1567-1575, 1986). In addition, nitrite antibodies have the advantage that they do not activate the complement system of mammalian sera and do not react with rheumatoid factors (Larsson, A., et al.). , J. Immunol, Methods, 108, 205-208, 1988).
- the present inventors have conducted intensive studies to establish a parental cell line for the production of a single monoclonal antibody, and found that the mouse B cell can be used as a mouse cell.
- all of these cells are resistant to HAT (hyposantin. (Lin, thymidine). Therefore, as a result of further research, a HAT-sensitive thymidine kinase-deficient cell line capable of stably growing was established. By fusing and culturing the nitric spleen cells immunized with this cell line, it was found that nitric monoclonal antibodies could be accumulated in the medium. (Japanese Patent Application No. 577-181, Int. Arch. Allergy Appl. Immunol., 88, 418-419 (1989)).
- a nitrile monoclonal antibody As mentioned above, if it is possible to supply a nitrile monoclonal antibody, it will be used as an effective means not only in the field of avian immunity but also in the field of mammalian immunity. Such a technique is limited to the one previously developed by the present inventor. However, the previously developed antibody-producing cells are inferior in stability, so that not only is it difficult to carry out subculture for a period of time, but also there is a problem that the antibody-producing ability is lost while the culture is continued for a period of time. . Since the antibody produced is IgM, the development of IgG-producing cells has been desired from the viewpoint of availability.
- the present invention has been made to solve such a problem, and it is possible to carry out subculture for a long period of time while retaining the antibody-producing ability, and to obtain a fused cell in which the produced antibody is an IgG class. It is intended for this purpose.
- the objective was to mutate the Nitri B-lymphoblastic cell line, isolate thymidine kinase-deficient cells using a medium containing a thymidine analog, and add the cells to the immune spleen cells of the Nitris. , And mutating the fused cells, and isolating a thymidine kinase-deficient cell line as a fusion cell using a medium containing a thymidine-like substance.
- Thymidin kinase-deficient cell lines with Ig-expressing ability which have been prepared by combining immunity spleen cells with nitrile cells in lymphoblasts Nitrile spleen cells immunized with the original are achieved by the fused IgG-producing cells that have been further fused, and have this IgG-producing ability.
- By culturing the fused cells in a medium it is possible to stably produce nitric IgG for a long period of time.
- FIG. 1 is a view showing a coloring pattern obtained by electrophoresis of a culture supernatant of an antibody-producing cell of the present invention and then examined by estam- blotting method.
- FIG. 2 is a diagram showing a color development pattern obtained by electrophoresis of a culture supernatant of the antibody-producing cells of the present invention and then examined by a ⁇ -stam blotting method.
- FIG. 3 is a diagram showing a color-developed state obtained by examining the above culture supernatant by the dot blot method.
- Thymidine kinase-deficient cell lines are obtained from nitrile periblasts. It does not matter what kind of nitrile, but for example, white leghorn, white rock, etc. can be used.
- Trilimpa blast cell lines can be obtained by denaturing nitrile or limpacells with Triletrovirus.
- the obtained autologous By-amba cell line is mutated to select thymidine kinase-deficient cells. Mutation may be performed by physical means such as ultraviolet irradiation, or by using a drug such as ethylmethansulfonate di-sodium diazine or ICR-191. You can do it. Isolation of thymidine kinase-deficient cells from mutant cells can be performed, for example, by culturing in a medium containing a thymidine-like substance such as trifluorothymidine or promodexidine.
- the thymidine kinase-deficient cell line thus obtained has self-propagating properties, but dies when cultured in HAT medium. Neither does it synthesize this Ig, as measured by the indirect fluorescent antibody method using an anti-Nitrile Ig antibody and a fluorescein-labeled secondary antibody. .
- This cell was designated as HU3R cell.
- Nitrite splenocytes immunized with HU3R cells are subjected to cell fusion.
- preparation of the splenocytes which were bred by injecting antigen, for example inactivation Men u mosquito Ssu Le disease c A di ⁇ van preparative I pulse and a monitor several surface two Wa Application Benefits may be out hysterectomy .
- Elaboration is performed by removing the spleen and using a known cell fusion method such as polyethylene glycol, electrofusion, or HVJ virus.
- the obtained fused cells are further mutated and a thymidine kinase-deficient strain is selected. These treatments are preferably performed after subculture until the growth of the fused cells is stabilized. Mutation treatment and selection of a thymidine kinase-deficient strain may be performed in the same manner as described above. Further, if necessary, the mutation means, conditions and the like may be changed. Expression of Ig can be carried out by detecting r ⁇ , “sin and L-sin, which are a part of Ig, and these ⁇ can be detected according to known methods.
- an indirect fluorescent antibody method using an anti-Nitrile Ig anti-rII antibody and a fluorescein-labeled second antibody, or a flow cytometry can be used.
- an indirect fluorescent antibody method using an anti-Nitrile Ig anti-rII antibody and a fluorescein-labeled second antibody, or a flow cytometry can be used.
- the expression of Ig has been confirmed.
- the nitrile: immune spleen cells are further fused to the thus obtained cell line having the Ig expression ability. Immunization and fusion methods may be as described above.
- the antigen used to develop immunity This antigen is considered to confer IgG-producing ability to the cells after fusion, and this antigen is selected according to the desired IgG.
- the culture can be carried out in the same manner as described above, and by culturing for about 1 to 30 minutes, IgG can be produced and stored in the culture supernatant.
- Known methods can be used for the method for separating IgG, including affinity chromatography, gel filtration, and ion-exchange chromatography. , Ethanol fractionation, rivanol fractionation, PEG fractionation and the like can be applied.
- avian reticuloendotheliosis virus a non-virus-producing B lymphoma blast cell line, RECC, was established in vitro by the transformation method from a nitrile in vitro.
- the HU3 strain is abbreviated as HU3.
- the L-producing hybridoma obtained by fusing the spleen cells of Nitori to a primary cell line. Using.
- the hybridoma was transformed into a thymidine kinase (TK) -deficient HU3R27 cell line (FERM P 10484, BP-3473), which was obtained by fusing spleen cells of a immunized chick with the inactively treated Newcastle disease virus (NDV). Indeed, production of L-protein was no longer observed over a long period of culture.
- TK thymidine kinase
- test cells were incubated in RPMI 1640 medium 25 supplemented with 5% FBS and placed in a 100 mm culture arc. This is a 38.5-(:., And incubated for 6 hours at 5% C 0 z ⁇ Nki Interview in Beta, was continued for 24 hours culture 3 ⁇ 4 in La Is the addition to the jar by that 600 g / and Do the EMS 24 After a lapse of time, the cells were washed with RPMI 1640 medium, incubated in RPMI 1640 medium 25 containing 10% FBS, and cultured for 3 days.3 days later, trifluoro thymidine (Cho FT; And the medium was further added to be 1 /. The grown cells are gradually grown in the same medium, and when the growth is stabilized, the TFT concentration in the medium is gradually increased, and the cells that grow stably at 10 g / are referred to as TK-deficient cells. Used.
- the TK-deficient cells were cloned by the soft agar method to obtain the following four clones.
- the medium used was IMDM medium containing 0.35% noble agar / 10% FBS-40% HU3R culture supernatant.
- Clones R27HI (FERM P-11543, FBRM BP-3475), R27H2 (FEMP-11544, FERM BP-3476), R27H3 (FERM P-11545, FERM BP- 3477) and R27H4 (FBRM P-11546, FERM BP-3478) and the parent cell line HU3R27 (FERM P-10484, FERM BP-3473) are shown in Table 1.
- R27H1 is 45.3 29.1. 51.7 17.29
- Ig secretion was determined by Dusten blot method, and cell surface Ig was determined by flow cytometry. The doubling time was determined by measuring the number of cells. As shown in Table 1, the four cell lines secreted Ig M, ⁇ , or L, respectively. Expression of L, ⁇ , and r proteins was observed on the cell surface.
- the horseshoe crab hemochanin (KLH) was administered to fuse the immunized spleen cells.
- Cell fusion was performed as follows. First, each cloned cell was collected in a polypropylene centrifuge tube, and washed three times with a serum-free RPMI 1640 medium. The clone cells and the spleen cells of the immunized with KLH were mixed at a ratio of 1: 5, respectively, and centrifuged at 267 G for 5 minutes. After centrifugation, the supernatant was completely aspirated and removed, and the bottom of the centrifuge tube was gently tapped to loosen fine aggregates, followed by warming in a 38'C water bath. PEG solution 1 which had been pre-warmed in step 38 was added to the cells over 1 minute.
- the solution was sometimes stirred at the tip of the bit while shaking the centrifuge tube back and forth and right and left.
- the mixture was allowed to stand for 1 minute.
- the centrifuge was performed. After centrifugation, the supernatant was gently aspirated off, and IMDM medium supplemented with 10% FBS was added to gently loosen cell pellets. Dispensed into 96 ⁇ et Rupure preparative Kumi ⁇ culture above cell suspension by 0.1 «e min, 38.5 Te, and cultured at 5% ⁇ 0 2 fin queue beta within one row Tsu name.
- the selection of the fused cells was performed as follows. First, 24 hours after the fusion, HAT (double-concentration) medium was added 100 ⁇ l to each plate of a 96-well plate for tissue culture. From the second day after the fusion, the medium was exchanged every 2 to 3 days with the HAT medium at a normal concentration, and the cells were cultured in the HAT medium for 10 to 10 days. After culturing in HAT medium for 10 to 14 days, HT medium was replaced and culturing was performed for 1 week.
- HAT double-concentration
- the cells were grown according to the cell growth using IMDM medium supplemented with 10% FBS.R27H1 (FERM P-11543, FERM BP-3475) and R27H2 (FERM P- 11544, FERM BP-3476), R27H3 (FERM P-11545, FERM BP-3477) .R27H4 (FERM P-11546, FERM BP-3478) clones were fused with immune splenocytes. At first, we had a hybrid dorma ⁇ However, it was possible to obtain hybridomas as each clone was subcultured.
- the culture supernatant obtained by culturing the hybridoma with R27H4 as the parent strain in IMDM medium supplemented with 10% FBS was subjected to polyacrylamide gel electrophoresis (SDS-PAGE), and western blotting was performed. The method was performed. In other words, after the electrophoresis, the DNA was transferred to nitrose cellulose paper, reacted with anti-nitrile, r and L antibodies labeled with S. vivosa monoxidase, and developed with dimethylaminobenzidine (DAB). At this time, the pattern shown in Fig. 1 was obtained.
- SDS-PAGE polyacrylamide gel electrophoresis
- the culture supernatant of a hybridoma (HU / Ch22-4, FERM-BP-3474) using R27H1 as a parent strain can be obtained by SDS-PAGE as described above, followed by ⁇ stamp opening.
- Fig. 2 shows the developed color patterns.
- 7 was also detected in addition to the protein, and the L protein was also detected, confirming that both IgG and IgM were produced.
- R 27 H series The production of Ig was observed in most of the fused cells obtained from the parental cells of B.
- IgM is derived from parent cells
- IgG is considered to be derived from spleen cells
- IgG is considered to have specificity for antibodies, and examined by ELISA and dot blot methods.
- 50 lig / mi KL ⁇ 50 / ⁇ was immobilized on the microplate, and the culture supernatant (50) was added.
- IgG production ability was determined by hybridomas (HU / Ch22-4, FERM P-11542, FBRM BP-3474) obtained using the R27H1 strain as a parent strain and the above-mentioned cloning.
- the obtained cells were cultured in 10% FBS-supplemented IMDM medium, and the culture supernatant collected every day after the fusion was measured by the above-mentioned EUSA method. The results are shown below.
- Table 2 shows the changes in absorbance of the other 9 clones similarly measured by ELISA.
- HD antibody is a heterophilic antibody reported by Hanganutziu and Deicher.This antibody is considered to be a causative antibody for serum sickness because it is elevated in the serum of patients injected with animal sera. Was. In recent years, however, it has been clarified that these are detected in various diseases such as irrespective of the injection of animal serum, and the HD antigen can be expected to be one of the most important diseases in these diseases.
- These antigens are glycolipids and glycoproteins that have N-glycolylneuraminic acid (NeuGc) as a non-reducing terminal sugar chain, and are effective against humans and nitros. Has been shown to be immunogenic.
- Several types of HD antigens are known. Some examples are shown below.
- H D 5 NeuGc (or2-3) Gal ( ⁇ l-4) GlcNAc () 91-3) Ga 1 ( ⁇ 1-4)
- H D 7 NeuGc (or2-3) Gal ( ⁇ 1-4) GlcNAc (1-3) Ga 1 (1-4)
- GlcNAc (1— 3) Gal (1-4) Glc-ceramide 4-0-acetyl-HD3 : 4-0- acety Neu NeuGc (or 2-3) Gal (1-4) Glc-ceram i de
- spleen cells immunized with the above-mentioned HD3 antigen and administered to the striatum and the R27H4 cell line (FERM P-11546, FERM BP-3478).
- the cell fusion method and the selection of cells were performed in the same manner as in Example 2.
- RPMI 1640 and IMDM medium were used as the medium for the culture after the consolidation of the cells.
- Table 3 a total of 443 hybridomas (HD antigen-specific antibody-producing hybridomas) were obtained, and the specificity of the culture supernatant for HD3 was determined. The specificity was confirmed in 73 of them, as revealed by the ELISA method.
- RPMI 1640 102/912 19/102 After cultivation for a long period of the hybridomas with specificity, especially after immobilization, immunoglobulin against HD3 is stable.
- the type of the immunoglobulin, the titer of the obtained culture supernatant, and its specificity were examined.
- the type of immunoglobulin produced from a hybridoma is determined by SDS-PAGE of the culture supernatant of the hybridoma. Determined by the Western blotting method of Example 2.
- Table 4 it became clear that the globulin type that also produced the two hybridomas examined was an IgG type.
- the titer of the hybridoma culture was measured by the same ELISA method as described above.
- the serially diluted hybridoma culture supernatant was reacted, and the HRP-labeled anti-nitridium immunoglobulin antibody was reacted. Thereafter, OPD was added and reacted.
- the titers were 1:80 and 1: 360, respectively, as shown in Table 4.
- the antigen specificity of HU / Ch6 1 360 IgG immunoglobulin was determined by TLC immunostaining.
- the TLC immobilizing method is used to transfer HD3, HD5, HD7 and 4-oAc-HD3 to TLC thin-layer chromatographic gels. After developing with a solvent, the mixture was dried and reacted with a hybridoma culture supernatant. After reaction with an HRP-labeled anti-nitriiminoglobulin antibody, color was developed with DAB.
- the two hybridoma cultures tested as shown in Table 5 react with HD.3HD5 * HD7 and 4-oAc-HD3 and HD3 and HD7. Became apparent.
- the antibody-producing cells of the present invention are capable of stably producing nitrile IgG over a long period of time, thereby opening the way to mass-produce nitrile IgG. is there.
- IgG is the largest and most versatile of immunoglobulins, and therefore, it is of great significance to be able to mass-produce low-density IgG cross-reactive with human IgG.
- Currently, a method for producing monoclonal antibodies using "I dairy animals such as mice and rats" is being implemented, but when antigens are derived from mammals such as humans, stimulation with human antigens is performed.
- the monoclonal antibody is not sensitized and a monoclonal antibody is not generated, and the present invention can provide a method for producing a monoclonal antibody even in such a system. Therefore, the present invention has the effect of further expanding the range of use of antibodies in a method for assaying biological trace components as a medicine and a K-bed test agent.
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Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE69130702T DE69130702T2 (de) | 1990-07-10 | 1991-07-10 | Geflügelspezifischen immunglobulin g produzierende hybridomas |
CA002066433A CA2066433A1 (en) | 1990-07-10 | 1991-07-10 | Chicken-specific immunoglobulin g-producing hybridoma |
EP91912328A EP0491057B1 (en) | 1990-07-10 | 1991-07-10 | Hybridoma which produces avian specific immunoglobulin g |
US07/838,775 US5411881A (en) | 1990-07-10 | 1991-07-10 | Chicken-specific immunoglobulin G-producing hybridoma |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18217490 | 1990-07-10 | ||
JP2/182174 | 1990-07-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1992001043A1 true WO1992001043A1 (fr) | 1992-01-23 |
Family
ID=16113640
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP1991/000923 WO1992001043A1 (fr) | 1990-07-10 | 1991-07-10 | Hydridomes produisant une immunoglobuline g aviaire specifique |
Country Status (7)
Country | Link |
---|---|
US (1) | US5411881A (ja) |
EP (1) | EP0491057B1 (ja) |
AT (1) | ATE175234T1 (ja) |
AU (1) | AU8212291A (ja) |
CA (1) | CA2066433A1 (ja) |
DE (1) | DE69130702T2 (ja) |
WO (1) | WO1992001043A1 (ja) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0539970B1 (en) * | 1991-10-30 | 1999-05-26 | Idemitsu Kosan Company Limited | Methods for producing human lymphocytes and human monoclonal antibodies, and human monoclonal antibodies produced thereby |
CA2146816A1 (en) | 1994-04-22 | 1995-10-23 | Joachim E. Klee | Process and composition for preparing a dental polymer product |
EP0737743A1 (en) * | 1995-04-14 | 1996-10-16 | Nkk Corporation | Thymidine kinase-lacking ouabain-resistant chicken hybridoma |
US6451984B1 (en) * | 1995-09-08 | 2002-09-17 | The United States Of America As Represented By The Secretary Of Agriculture | Chicken monoclonal antibodies specific for coccidial antigens involved in invasion of host lymphocytes |
WO1997016537A1 (en) * | 1995-10-30 | 1997-05-09 | Spectral Diagnostics, Inc. | Stable chicken b-cell line and method of use thereof |
US6143559A (en) * | 1996-11-18 | 2000-11-07 | Arch Development Corporation | Methods for the production of chicken monoclonal antibodies |
CU22731A1 (es) * | 1998-02-05 | 2002-02-28 | Centro Inmunologia Molecular | Anticuerpo monoclonal que reconoce el oligosacárido ácido siálico n´glicolilado-galactosa-glucosa (ngcneu-gal-glu) en tumores malignos y composiciones farmacéuticas que los contienen |
CA2634294A1 (en) | 2000-08-03 | 2002-02-14 | Therapeutic Human Polyclonals, Inc. | Production of humanized antibodies in transgenic animals |
WO2005007696A2 (en) | 2003-07-15 | 2005-01-27 | Therapeutic Human Polyclonals, Inc. | Humanized immunoglobulin loci |
US20090053210A1 (en) | 2006-09-01 | 2009-02-26 | Roland Buelow | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals |
AU2008259939B2 (en) | 2007-06-01 | 2014-03-13 | Open Monoclonal Technology, Inc. | Compositions and methods for inhibiting endogenous immunoglobulin genes and producing transgenic human idiotype antibodies |
CN113150121A (zh) | 2010-08-02 | 2021-07-23 | 瑞泽恩制药公司 | 制造包含vl结构域的结合蛋白的小鼠 |
DK2931030T4 (da) | 2012-12-14 | 2024-04-22 | Omniab Inc | Polynukleotider, der koder for graver-antistoffer med humane idiotyper, og dyr, der omfatter disse |
CA2941514A1 (en) | 2014-03-21 | 2015-09-24 | Regeneron Pharmaceuticals, Inc. | Vl antigen binding proteins exhibiting distinct binding characteristics |
CA2942697A1 (en) | 2014-03-21 | 2015-09-24 | Lynn Macdonald | Non-human animals that make single domain binding proteins |
EP3271403A1 (en) | 2015-03-19 | 2018-01-24 | Regeneron Pharmaceuticals, Inc. | Non-human animals that select for light chain variable regions that bind antigen |
CN110662421B (zh) | 2017-01-19 | 2023-03-24 | 欧莫诺艾比公司 | 来自具有多个重链免疫球蛋白基因座的转基因啮齿类动物的人抗体 |
EP3801010A4 (en) | 2018-06-08 | 2022-02-23 | Crystal Bioscience Inc. | TRANSGENIC ANIMAL TO PRODUCE DIVERSIFIED ANTIBODIES WITH THE SAME LIGHT CHAIN I |
EP3897117A1 (en) | 2018-12-21 | 2021-10-27 | Compass Therapeutics LLC | Transgenic mouse expressing common human light chain |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63270699A (ja) * | 1987-04-28 | 1988-11-08 | Konica Corp | モノクロ−ナル抗体 |
JPS63304995A (ja) * | 1987-06-04 | 1988-12-13 | Konica Corp | モノクローナル抗体 |
JPH02186980A (ja) * | 1989-01-12 | 1990-07-23 | Nkk Corp | ニワトリチミジンキナーゼ欠損bリンパ芽細胞株 |
-
1991
- 1991-07-10 AT AT91912328T patent/ATE175234T1/de not_active IP Right Cessation
- 1991-07-10 US US07/838,775 patent/US5411881A/en not_active Expired - Lifetime
- 1991-07-10 EP EP91912328A patent/EP0491057B1/en not_active Expired - Lifetime
- 1991-07-10 DE DE69130702T patent/DE69130702T2/de not_active Expired - Fee Related
- 1991-07-10 WO PCT/JP1991/000923 patent/WO1992001043A1/ja active IP Right Grant
- 1991-07-10 AU AU82122/91A patent/AU8212291A/en not_active Abandoned
- 1991-07-10 CA CA002066433A patent/CA2066433A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63270699A (ja) * | 1987-04-28 | 1988-11-08 | Konica Corp | モノクロ−ナル抗体 |
JPS63304995A (ja) * | 1987-06-04 | 1988-12-13 | Konica Corp | モノクローナル抗体 |
JPH02186980A (ja) * | 1989-01-12 | 1990-07-23 | Nkk Corp | ニワトリチミジンキナーゼ欠損bリンパ芽細胞株 |
Non-Patent Citations (3)
Title |
---|
"Hybridoma" Vol. 9, No. 4 (1990) p. 331-350. * |
(BIKEN J.), Vol. 25, p. 47-50 (1982). * |
Journal of Biochemistry (Tokyo) Vol. 95, p. 785-794 (1984). * |
Also Published As
Publication number | Publication date |
---|---|
AU8212291A (en) | 1992-02-04 |
US5411881A (en) | 1995-05-02 |
ATE175234T1 (de) | 1999-01-15 |
EP0491057A4 (en) | 1993-05-26 |
EP0491057A1 (en) | 1992-06-24 |
CA2066433A1 (en) | 1992-01-11 |
EP0491057B1 (en) | 1998-12-30 |
DE69130702T2 (de) | 1999-05-27 |
DE69130702D1 (de) | 1999-02-11 |
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