WO1991019814A3 - Sondes d'adn et amorces pour la detection de b. burgdorferi au moyen de l'amplification enzymatique du genome - Google Patents

Sondes d'adn et amorces pour la detection de b. burgdorferi au moyen de l'amplification enzymatique du genome Download PDF

Info

Publication number
WO1991019814A3
WO1991019814A3 PCT/US1991/004190 US9104190W WO9119814A3 WO 1991019814 A3 WO1991019814 A3 WO 1991019814A3 US 9104190 W US9104190 W US 9104190W WO 9119814 A3 WO9119814 A3 WO 9119814A3
Authority
WO
WIPO (PCT)
Prior art keywords
burgdorferi
primers
detection
chain reaction
polymerase chain
Prior art date
Application number
PCT/US1991/004190
Other languages
English (en)
Other versions
WO1991019814A2 (fr
Inventor
Roger N Picken
Harryl C Ammons
Original Assignee
Baxter Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Diagnostics Inc filed Critical Baxter Diagnostics Inc
Priority to DE69117885T priority Critical patent/DE69117885T2/de
Priority to EP91912625A priority patent/EP0487709B1/fr
Priority to JP3511770A priority patent/JPH074276B2/ja
Publication of WO1991019814A2 publication Critical patent/WO1991019814A2/fr
Priority to NO92920606A priority patent/NO920606L/no
Priority to FI920636A priority patent/FI920636A0/fi
Publication of WO1991019814A3 publication Critical patent/WO1991019814A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Measuring Magnetic Variables (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
  • Lubricants (AREA)
  • Compositions Of Oxide Ceramics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

On utilise des amorces d'acides nucléiques de séquence complémentaire de parties du gène codant une région semiconservée de flagelline de B. burgdorferi afin d'amplifier des séquences cibles d'acides nucléiques extraites de sites infectieux de Lyme borreliosis, lesquelles sont ensuite sondées à l'aide d'un traceur d'acide nucléique conjugé à des molécules génératrices de signaux permettant la détection hautement spécifique de B. burgdorferi.
PCT/US1991/004190 1990-06-15 1991-06-12 Sondes d'adn et amorces pour la detection de b. burgdorferi au moyen de l'amplification enzymatique du genome WO1991019814A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE69117885T DE69117885T2 (de) 1990-06-15 1991-06-12 Dns-sonden und primer zur erkennung von b. burgdorferi unter verwendung der polymerase-kettenreaktion
EP91912625A EP0487709B1 (fr) 1990-06-15 1991-06-12 Sondes d'adn et amorces pour la detection de b. burgdorferi au moyen de l'amplification enzymatique du genome
JP3511770A JPH074276B2 (ja) 1990-06-15 1991-06-12 複製連鎖反応を利用してｂ．ブルグドルフエリを検出するためのｄｎａプローブおよびプライマー
NO92920606A NO920606L (no) 1990-06-15 1992-02-14 Dna-prober og -primere for paavisning av b. burgdorferi ved hjelp av polymerasekjedereaksjonen
FI920636A FI920636A0 (fi) 1990-06-15 1992-02-14 Dna-sonder och primers foer detektion av b. burgdorferi medelst foer detektion av b.burgdorferi medelst polymeraskedjereaktionen.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US53895790A 1990-06-15 1990-06-15
US69118891A 1991-04-25 1991-04-25
US691,188 1991-04-25
US538,957 1991-04-25

Publications (2)

Publication Number Publication Date
WO1991019814A2 WO1991019814A2 (fr) 1991-12-26
WO1991019814A3 true WO1991019814A3 (fr) 1992-04-02

Family

ID=27065973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/004190 WO1991019814A2 (fr) 1990-06-15 1991-06-12 Sondes d'adn et amorces pour la detection de b. burgdorferi au moyen de l'amplification enzymatique du genome

Country Status (16)

Country Link
EP (1) EP0487709B1 (fr)
JP (2) JPH074276B2 (fr)
CN (1) CN1061245A (fr)
AT (1) ATE135413T1 (fr)
AU (1) AU638348B2 (fr)
CA (1) CA2064582A1 (fr)
CS (1) CS183591A3 (fr)
DE (1) DE69117885T2 (fr)
DK (1) DK0487709T3 (fr)
ES (1) ES2087298T3 (fr)
FI (1) FI920636A0 (fr)
HU (2) HU216204B (fr)
IE (1) IE62797B1 (fr)
NO (1) NO920606L (fr)
PL (1) PL293741A1 (fr)
WO (1) WO1991019814A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05506987A (ja) * 1990-03-07 1993-10-14 エフ．ホフマン　―　ラ　ロシュ　アーゲー ライム病の診断方法
WO1993003184A1 (fr) * 1991-08-09 1993-02-18 Mccann Associates, Inc. Procede et composition permettant de detecter la maladie de lyme
US5932220A (en) * 1995-05-08 1999-08-03 Board Of Regents University Of Texas System Diagnostic tests for a new spirochete, Borrelia lonestari sp. nov.
WO2003029450A1 (fr) * 2001-09-28 2003-04-10 Københavns Universitet Sequences d'acide nucleique codant lysk a partir de differentes souches de borrelia et leurs utilisations
CN101886113B (zh) * 2009-05-13 2012-10-17 中国农业科学院兰州兽医研究所 蜱体内检测莱姆病病原的检测试剂盒
FR3056991B1 (fr) * 2016-10-03 2020-11-27 C A L Laboratoire De Biologie Veterinaire Methode et kit de detection, de discrimination et identification d'especes de borrelies presentes dans un echantillon d'origine humaine ou animale

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009870A1 (fr) * 1989-12-22 1991-07-11 Mikrogen Molekularbiologische Entwicklungs-Gmbh Proteines immunologiquement actives de borrelia burgdorferi, coffrets d'analyse connexes et vaccins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009870A1 (fr) * 1989-12-22 1991-07-11 Mikrogen Molekularbiologische Entwicklungs-Gmbh Proteines immunologiquement actives de borrelia burgdorferi, coffrets d'analyse connexes et vaccins

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Infection and Immunity, volume 58, no. 6, June 1990, American Society for Microbiology, R. Wallich et al.: "The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene", pages 1711-1719, see the whole article, especially last paragraph of page 1712 *
Journal of Clinical Microbiology, volume 28, no. 6, June 1990, American Society for Microbiology, D.C. Malloy et al.: "Detection of Borrelia burgdorferi using the polymerase chain reaction", pages 1089-1093, see the whole article *
Nucleic Acids Research, volume 17, no. 9, May 1989, IRL Press, G.S. Gassmann et al.: "Nucleotide sequence of a gene encoding the Borrelia burgdorferi flagellin", page 3590, see the whole article *
The Journal of Infectious Deseases, volume 160, no. 6, December 1989, P.A. Rosa et al.: "A specific and sensitive assay for the lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction", pages 1018-1029, see the whole article *

Also Published As

Publication number Publication date
CN1061245A (zh) 1992-05-20
HU216204B (hu) 1999-05-28
AU8182291A (en) 1992-01-07
DK0487709T3 (da) 1996-04-01
CA2064582A1 (fr) 1991-12-16
ATE135413T1 (de) 1996-03-15
DE69117885D1 (de) 1996-04-18
JPH05501652A (ja) 1993-04-02
HUT60529A (en) 1992-09-28
JPH074276B2 (ja) 1995-01-25
HU9200858D0 (en) 1992-05-28
JPH07163400A (ja) 1995-06-27
EP0487709A1 (fr) 1992-06-03
FI920636A0 (fi) 1992-02-14
HU910858D0 (en) 1991-09-30
IE912022A1 (en) 1991-12-18
ES2087298T3 (es) 1996-07-16
WO1991019814A2 (fr) 1991-12-26
DE69117885T2 (de) 1996-11-28
HUT63006A (en) 1993-06-28
JP2657628B2 (ja) 1997-09-24
PL293741A1 (en) 1993-01-25
IE62797B1 (en) 1995-03-08
NO920606D0 (no) 1992-02-14
NO920606L (no) 1992-02-14
AU638348B2 (en) 1993-06-24
EP0487709B1 (fr) 1996-03-13
CS183591A3 (en) 1992-01-15

Similar Documents

Publication Publication Date Title
JP2678597B2 (ja) 分離されたオリゴヌクレオチド類を電気泳動的に検出する方法
CA2036946A1 (fr) Molecules de liaison pour indexation
EP1961826A3 (fr) Procédé de séquençage direct d'acides nucléiques
NO934235D0 (no) Anvendelse av nukleinsyreanaloger for diagnostiske og analytiske fremgangsm}ter
ES2146215T3 (es) Ensayos rapidos para productos de amplificacion.
KR920701478A (ko) 핵산서열의 증폭방법
EP0450594A3 (en) Dna probe signal amplification
AU2485688A (en) Non-nucleotide linking reagents for nucleotide probes
ES2052607T3 (es) Metodos y kits para detectar secuencias de acidos nucleicos.
AU8127494A (en) Chimeric proteins comprising borrelia polypeptides: uses therefor
ES2151002T3 (es) Deteccion del treponema pallidum y haemophilus ducreyi.
WO1991019814A3 (fr) Sondes d'adn et amorces pour la detection de b. burgdorferi au moyen de l'amplification enzymatique du genome
WO2000061800A3 (fr) Procede d'analyse de melanges reactionnels d'aflp® a l'aide de techniques d'extension d'amorce
Ogawa et al. RNA-linked nascent DNA pieces in phage T7-infected Escherichia coli. III. Detection of intact primer RNA
EP0293079A3 (fr) Vaccin contre chlamydia
WO1994012669A3 (fr) Detection d'adn contaminants par polymerisation en chaine
EP1258731A3 (fr) Support solid réactif pour la détection de fragments d'ADN
ES2103312T3 (es) Secuencia de polinucleotidos de la salmonella.
AU6718894A (en) Hippuricase gene
KR920019935A (ko) 쭈쭈가무시균의 종 특이 dna염기서열 및 이를 이용한 쭈쭈가무시병의 진단방법
ES2021482A6 (es) Procedimiento de preparacion de oligonucleotidos sinteticos y de amplificacion genica de sondas moleculares de adn especifico del fenoma macho de rumiantes.
RU94024845A (ru) Усовершенствованный способ дифференциации вакцинных и патогенных штаммов бруцелл крупного рогатого скота

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA FI HU JP NO PL SU

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 1991912625

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2064582

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 920636

Country of ref document: FI

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA FI HU JP NO PL SU

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

WWP Wipo information: published in national office

Ref document number: 1991912625

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 1991912625

Country of ref document: EP