KR920019935A - 쭈쭈가무시균의 종 특이 dna염기서열 및 이를 이용한 쭈쭈가무시병의 진단방법 - Google Patents

쭈쭈가무시균의 종 특이 dna염기서열 및 이를 이용한 쭈쭈가무시병의 진단방법 Download PDF

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KR920019935A
KR920019935A KR1019910006791A KR910006791A KR920019935A KR 920019935 A KR920019935 A KR 920019935A KR 1019910006791 A KR1019910006791 A KR 1019910006791A KR 910006791 A KR910006791 A KR 910006791A KR 920019935 A KR920019935 A KR 920019935A
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South Korea
Prior art keywords
species
specific dna
schizobacteria
base sequences
diagnostic methods
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KR1019910006791A
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English (en)
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KR930010768B1 (ko
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장우현
김익상
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허영섭
주식회사 녹십자
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Priority to KR1019910006791A priority Critical patent/KR930010768B1/ko
Publication of KR920019935A publication Critical patent/KR920019935A/ko
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Publication of KR930010768B1 publication Critical patent/KR930010768B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

내용 없음

Description

주쭈가무시균의 종 특이 DNA 염기서열 및 이를 이용한 쭈쭈가무시병의 진단방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1도, pKT12의 삽입 DNA에 있어서의 합성 오리고 뉴클레오티드 프라이머(Oligoucleotide primers)의 위치와 프라이머의 염기서열,
제 2도, DNA 중합효소 연쇄반응의 표적 DNA의 염기서열,
제 3도, pc4와 pc5 프라이머를 사용한 PCR 방법에 의하여 pKT12 프리스미드를 35회 증폭시켜 아가로스(1.4%)겔 정기영동한 결과.

Claims (3)

  1. DNA 중합효소 연쇄반응법을 이용하여 쭈쭈가무시병을 진단하는데 이용되는 하기의 388개 염기서열로 된 것을 특징으로 하는 쭈쭈가무시균의 종 특이 DNA 염기서열
  2. 제 1항의 표적 DNA의 증폭을 위하여 이용되는 각 20개로 된 하기의 염기서열을 가지는 것을 특징으로 하는 프라이머(pc4)와 리버스프라이머(pc5)
    pc4 ; ACTAT CTAGC ATCGG ATCTG
    pc5 ; TTGGT TATAA TGAGC AGTTA
  3. 제 2항의 프라이머와 리버스프라이머를 이용하여 DNA 중합효소 연쇄반응으로 쭈쭈가무시병을 진단하는 것을 특징으로 하는 진단방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910006791A 1991-04-26 1991-04-26 쭈쭈가무시균의 종 특이 dna 염기서열 KR930010768B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910006791A KR930010768B1 (ko) 1991-04-26 1991-04-26 쭈쭈가무시균의 종 특이 dna 염기서열

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910006791A KR930010768B1 (ko) 1991-04-26 1991-04-26 쭈쭈가무시균의 종 특이 dna 염기서열

Publications (2)

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KR920019935A true KR920019935A (ko) 1992-11-20
KR930010768B1 KR930010768B1 (ko) 1993-11-10

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KR1019910006791A KR930010768B1 (ko) 1991-04-26 1991-04-26 쭈쭈가무시균의 종 특이 dna 염기서열

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KR (1) KR930010768B1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100550463B1 (ko) * 2003-10-20 2006-02-13 학교법인 건국대학교 리케챠와 쭈쭈가무시 균의 동시 검출용 프라이머

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100528673B1 (ko) * 2003-04-09 2005-11-16 주식회사 퓨쳐시스템 비상시 데이터 소거 장치

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100550463B1 (ko) * 2003-10-20 2006-02-13 학교법인 건국대학교 리케챠와 쭈쭈가무시 균의 동시 검출용 프라이머

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KR930010768B1 (ko) 1993-11-10

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