WO1991005255A1 - Diagnostic d'infections pulmonaires par analyse des constituants volatils et non volatils de l'air inhale - Google Patents

Diagnostic d'infections pulmonaires par analyse des constituants volatils et non volatils de l'air inhale Download PDF

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Publication number
WO1991005255A1
WO1991005255A1 PCT/EP1990/001638 EP9001638W WO9105255A1 WO 1991005255 A1 WO1991005255 A1 WO 1991005255A1 EP 9001638 W EP9001638 W EP 9001638W WO 9105255 A1 WO9105255 A1 WO 9105255A1
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WIPO (PCT)
Prior art keywords
substances
volatile
analysis
air
methods
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PCT/EP1990/001638
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German (de)
English (en)
Inventor
Lutz Scheideler
Hugo Hämmerle
Otto Inacker
Original Assignee
Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen
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Application filed by Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen filed Critical Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen
Publication of WO1991005255A1 publication Critical patent/WO1991005255A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Definitions

  • lung cell types e.g. of the various cell types of the lung epithelium (pneumocytes type I and II) or of the interstitial s.
  • the first indicators at the cellular level can be the impairment of specific metabolic performance of certain cells (e.g. surfactant release of pneumocytes type II) or the activation of the body's own defense mechanisms (release of mediators).
  • the object of the present application is therefore on the one hand to obtain sample material for the determination of diagnostically relevant constituents of the broncho-alveolar boundary fluid under conditions which are not stressful for the patient and possibly also reasonable within the framework of a preventive examination.
  • analytical methods should be provided for the qualitative and quantitative discontinuous and continuous determination of these components.
  • Breathing gas analyzes e.g. using mass spectrometric methods have so far only been used on volatile, low molecular weight, mostly organic compounds, such as Acetone, methyl ethyl ketone, propanol (Gordon 1985), pyridine (Kostelc 1980), dichloromethane, toluene, styrene (Wilson 1981), ammonia, acetaldehyde and ethanol (Lovett 1979).
  • organic compounds such as Acetone, methyl ethyl ketone, propanol (Gordon 1985), pyridine (Kostelc 1980), dichloromethane, toluene, styrene (Wilson 1981), ammonia, acetaldehyde and ethanol (Lovett 1979).
  • the invention thus relates to a method for diagnosing the
  • the non-volatile components from approx. 750 l i.e. from 250 breaths
  • the non-volatile components from approx. 750 l were enriched by condensation in a cold trap at -196 ° C and subsequent freeze-drying.
  • biochemical methods protein separation by two-dimensional SDS gel electrophoresis (sodium dodecyl sulfate) followed by silver staining
  • proteins up to a molecular weight of 60,000 D could be found (Dalton) can be detected.
  • non-volatile substances e.g. Macromolecules, in particular proteins
  • Macromolecules in particular proteins
  • test person's saliva sample (approx. 300 ⁇ l) was also first freeze-dried, then taken up in buffer and electrophoresed.
  • the separation of the proteins from the air and saliva contained in the applied substance was carried out by two-dimensional polyacrylamide gel electrophoresis (see Fig. 3).
  • Sheets I - IV are accompanied by illustrations that have the following meaning:
  • Fig. 1 Secondary ion mass spectra of breathed sample plates. About 1 cm 2 large, freshly vaporized silver indium plates were placed on a cooling table (-196 ° C) and a) breathed through a glass tube with 6 breaths or b) exposed to the ambient air for the same time. The samples were freeze-dried on the cryotable by applying an ultra-high vacuum and then measured in the secondary ion mass spectrometer.
  • Fig. 2 Freeze-dried substance f t of approximately 750 1 Atemlu.
  • the non-volatile components of the breathing air were first frozen out from 250 breaths of a subject in a cold trap at -196 ° C. ( ⁇ 15 ml of aqueous condensate) and then freeze-dried.
  • Fig. 3 Gel electrophoresis diagrams of breathing air and saliva samples of a subject after separation of the proteins
  • Fig. 4 Comparison of the protein pattern (2D-SDS gels, Fig. 3) of breath and saliva samples. literature

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

Le film de liquide garnissant les poumons et les voies respiratoires (liquide alvéolaire ou sécrétion bronchique, crachat) renferme des médiateurs et des produits métaboliques volatils et non volatils dont certains sont d'une importance diagnostique considérable. Des substances primaires non volatiles peuvent pénétrer sous forme d'aérosols dans l'air exhalé. La détermination de ces substances dans l'air exhalé humain peut être utilisée, éventuellement conjointement avec des substances volatiles, pour diagnostiquer des maladies.
PCT/EP1990/001638 1989-09-30 1990-09-28 Diagnostic d'infections pulmonaires par analyse des constituants volatils et non volatils de l'air inhale WO1991005255A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19893932784 DE3932784C3 (de) 1989-09-30 1989-09-30 Verfahren zur Gewinnung nichtflüchtiger Substanzen als analytisches Probenmaterial aus der menschlichen Atemluft
DEP3932784.1 1989-09-30

Publications (1)

Publication Number Publication Date
WO1991005255A1 true WO1991005255A1 (fr) 1991-04-18

Family

ID=6390625

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1990/001638 WO1991005255A1 (fr) 1989-09-30 1990-09-28 Diagnostic d'infections pulmonaires par analyse des constituants volatils et non volatils de l'air inhale

Country Status (2)

Country Link
DE (1) DE3932784C3 (fr)
WO (1) WO1991005255A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0669531A1 (fr) * 1994-02-24 1995-08-30 Kraft Foods, Inc. Méthode et appareil de mesure de produits volatils issus de produits alimentaires
WO1995031721A1 (fr) * 1994-05-13 1995-11-23 Filt Forschungsgesellschaft Für Lungen- Und Thoraxerkrankungen Mbh Procede et dispositif permettant de recueillir un condensat de souffle expire
WO1997004310A1 (fr) * 1995-07-24 1997-02-06 Medium - Sensor Gmbh Dispositif pour la recuperation de substances contenues dans l'air expire
WO1999028743A1 (fr) * 1997-12-03 1999-06-10 Ufz-Umweltforschungszentrum Leipzig-Halle Gmbh Dispositif pour etudier les maladies des voies respiratoires et agents diagnostiques
DE102005026933A1 (de) * 2005-06-06 2006-12-07 Filt Lungen- Und Thoraxdiagnostik Gmbh Verfahren und Vorrichtung zur Messung und Analyse von Bestandteilen von exhaliertem Atemgas
DE19717717B4 (de) * 1997-04-18 2007-08-02 InViTek Gesellschaft für Biotechnik & Biodesign mbH Verfahren zur nichtinvasiven Erkennung bösartiger Tumoren der Lunge

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19920233C2 (de) * 1999-05-03 2002-11-07 Siemens Ag Vorrichtung zur Optimierung der Behandlung von Nierenleiden
DE19951204C2 (de) * 1999-10-15 2002-08-08 Filt Forschungsgesellschaft Fu Verfahren zur Analyse der Inhaltsstoffe der Ausatemluft
DE10232849A1 (de) * 2002-07-19 2004-02-12 Abb Patent Gmbh Gasanalyseeinrichtung zur Qualitätsüberwachung eines gasförmigen Stoffes oder Stoffgemisches, insbesondere Luft
DE10232850A1 (de) * 2002-07-19 2004-02-12 Abb Patent Gmbh Mobile Analyseeinrichtung
DE102006039140B4 (de) * 2006-08-16 2013-02-21 Aerocrine Ab Vorrichtung, deren Verwendung und Verfahren zum Fraktionieren des Exspirationsvolumens
DE102008030158A1 (de) * 2008-06-27 2010-02-11 Dina Levina Vorrichtung zur Gewinnung von Atemluftkondensat

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649199A (en) * 1970-03-26 1972-03-14 Varian Associates Method for detecting trace quantities of an organic drug material in a living animal
GB2045930A (en) * 1979-03-19 1980-11-05 Draegerwerk Ag Breath test device
US4772559A (en) * 1985-10-10 1988-09-20 Monell Chemical Senses Center Method of detecting the presence of bronchogenic carcinoma by analysis of expired lung air

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2034468B (en) * 1978-11-03 1983-02-09 Jones T Gas sampling devices
JP2620787B2 (ja) * 1987-07-31 1997-06-18 株式会社ジャルコ 臭気測定器

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649199A (en) * 1970-03-26 1972-03-14 Varian Associates Method for detecting trace quantities of an organic drug material in a living animal
GB2045930A (en) * 1979-03-19 1980-11-05 Draegerwerk Ag Breath test device
US4772559A (en) * 1985-10-10 1988-09-20 Monell Chemical Senses Center Method of detecting the presence of bronchogenic carcinoma by analysis of expired lung air

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Analytical Chemistry, Band 55, Nr. 4, April 1983, American Chemical Society, F.M. Benoit et al.: "Breath analysis by atmospheric pressure ionization mass spectrometry", Seiten 805-807 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0669531A1 (fr) * 1994-02-24 1995-08-30 Kraft Foods, Inc. Méthode et appareil de mesure de produits volatils issus de produits alimentaires
WO1995031721A1 (fr) * 1994-05-13 1995-11-23 Filt Forschungsgesellschaft Für Lungen- Und Thoraxerkrankungen Mbh Procede et dispositif permettant de recueillir un condensat de souffle expire
WO1997004310A1 (fr) * 1995-07-24 1997-02-06 Medium - Sensor Gmbh Dispositif pour la recuperation de substances contenues dans l'air expire
DE19717717B4 (de) * 1997-04-18 2007-08-02 InViTek Gesellschaft für Biotechnik & Biodesign mbH Verfahren zur nichtinvasiven Erkennung bösartiger Tumoren der Lunge
WO1999028743A1 (fr) * 1997-12-03 1999-06-10 Ufz-Umweltforschungszentrum Leipzig-Halle Gmbh Dispositif pour etudier les maladies des voies respiratoires et agents diagnostiques
DE102005026933A1 (de) * 2005-06-06 2006-12-07 Filt Lungen- Und Thoraxdiagnostik Gmbh Verfahren und Vorrichtung zur Messung und Analyse von Bestandteilen von exhaliertem Atemgas
EP1731095A2 (fr) 2005-06-06 2006-12-13 FILT Lungen- und Thoraxdiagnostik GmbH Mesure et analyse de composants du gaz respiratoire exhalé
DE102005026933B4 (de) * 2005-06-06 2010-09-23 Filt Lungen- Und Thoraxdiagnostik Gmbh Verfahren und Verwendung einer Vorrichtung zur Durchführung des Verfahrens zur Messung und Analyse von Bestandteilen von exhaliertem Atemgas

Also Published As

Publication number Publication date
DE3932784C3 (de) 2002-08-01
DE3932784A1 (de) 1991-04-11
DE3932784C2 (de) 1997-05-15

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