Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/04—Preserving or maintaining viable microorganisms
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/20—Bacteria; Culture media therefor
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
  • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Definitions

• the present invention relates to a new transport medium for microorganisms, in particular gram-negative, gram-positive and anaerobic bacteria.
• the known transport media have proven to be quite useful for a normal transport time of about 12-24 hours and for aerobic and less sensitive anaerobic bacteria. With longer transport times of 48-72 hours as they can occur in unfavorable traffic conditions or on weekends and for
• the task was therefore to find new transport media in which particularly sensitive pathogens can be kept in a reproductive state for a long time.
• composition of the agent according to the invention has strong similarities to the known transport medium from Amies, which also contains agar, sodium, potassium, calcium and magnesium chloride and sodium thioglucolate, but the sodium phosphate buffer from Amies is replaced by a sodium glycerol phosphate buffer and additionally the mixture enriched with meat extract.
• the new medium has a slightly reduced content due to the content of thioglucolate, so that in particular in connection with an oxygen-free protection containing nitrogen or carbon dioxide gas atmosphere, anaerobic bacteria are particularly protected.
• the addition of meat extract causes a weak supply of nutrients, which obviously favors the persistence of the pathogens, but without growth and initiate an increase.
• agar serves as a gel-forming agent in order to give the mixture the necessary strength. Quantities of 2-10 g / l are useful for this purpose, 5 g / 1 are preferred.
• Sodium thioglucolate and sodium glycerol phosphate in turn serve on the one hand to make the mixture weakly reducing, which, as stated above, favors the anaerobic pathogens in particular, and on the other hand the pH value of the mixture of approx. 7.3 + 0, which is important for the viability of the bacteria, 1 to hold or adjust.
• the mixture according to the invention is excellently suitable for the production of prefabricated transport containers which, if appropriate, are filled with sterile filling of the mixture into the usual transport tubes and vials and with a moisture-proof closure in a protective gas atmosphere and storage at normal temperatures between 6 and 20 ° C are still fully usable after 6-12 months.
• the invention can be any suitable material. Depending on the intended purpose, the invention can be any suitable material.
• Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen.
• Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen.
• antimicrobial additives such as vancomycin, kanamycin, nystatin or trimethoprim.
• compositions according to the invention were compared against a commercial transport medium according to "Amies of the following composition: Agar no. 1 7.5
• the preservative properties of the two transport media were compared after periods of 24, 48, 72 and 96 hours against a representative number of different gram-positive, gram-negative and anaerobic bacteria.
• the results are summarized in the following table, where the results represent the mean values from a number of n samples.
• cultures the pathogen in physiological saline solution are brought to a concentration of 5-10,000 germs / ml and 0.1 ml of this reading is sprayed onto the agar surface of the transport bottle.
• the vials are each sealed with a rubber membrane and filled with a protective gas atmosphere for anaerobic bacteria.
• 0.1 ml of the mixture is removed from agar plates which are suitable for the microorganism in question, spread out and incubated at 37 °.
• the shelf life of the pathogens in the transport medium is assessed semi-quantitatively by evaluating the number of colonies in the classes: abundant (+++), moderate (++), isolated (+) and no germ growth (-).

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Organic Chemistry (AREA)
• Zoology (AREA)
• Wood Science & Technology (AREA)
• Genetics & Genomics (AREA)
• Biotechnology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• General Health & Medical Sciences (AREA)
• Microbiology (AREA)
• General Engineering & Computer Science (AREA)
• Biochemistry (AREA)
• Virology (AREA)
• Biomedical Technology (AREA)
• Tropical Medicine & Parasitology (AREA)
• Medicinal Chemistry (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Biophysics (AREA)
• Analytical Chemistry (AREA)
• Physics & Mathematics (AREA)
• Toxicology (AREA)
• Immunology (AREA)
• Molecular Biology (AREA)
• Medicines Containing Material From Animals Or Micro-Organisms (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

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Publications (1)

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Cited By (1)

Citations (3)

• 1988
  • 1988-04-13 DE DE19883812194 patent/DE3812194A1/de active Granted
• 1989
  • 1989-08-25 WO PCT/EP1989/001003 patent/WO1991002786A1/de not_active Ceased

Patent Citations (3)

Non-Patent Citations (1)

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