WO1991002786A1 - Transport medium for microorganisms - Google Patents

Transport medium for microorganisms Download PDF

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Publication number
WO1991002786A1
WO1991002786A1 PCT/EP1989/001003 EP8901003W WO9102786A1 WO 1991002786 A1 WO1991002786 A1 WO 1991002786A1 EP 8901003 W EP8901003 W EP 8901003W WO 9102786 A1 WO9102786 A1 WO 9102786A1
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sodium
chloride
transport
transport medium
agar
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PCT/EP1989/001003
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German (de)
French (fr)
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Wolfgang Klietmann
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Wolfgang Klietmann
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Publication of WO1991002786A1 publication Critical patent/WO1991002786A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Definitions

  • the present invention relates to a new transport medium for microorganisms, in particular gram-negative, gram-positive and anaerobic bacteria.
  • the known transport media have proven to be quite useful for a normal transport time of about 12-24 hours and for aerobic and less sensitive anaerobic bacteria. With longer transport times of 48-72 hours as they can occur in unfavorable traffic conditions or on weekends and for
  • the task was therefore to find new transport media in which particularly sensitive pathogens can be kept in a reproductive state for a long time.
  • composition of the agent according to the invention has strong similarities to the known transport medium from Amies, which also contains agar, sodium, potassium, calcium and magnesium chloride and sodium thioglucolate, but the sodium phosphate buffer from Amies is replaced by a sodium glycerol phosphate buffer and additionally the mixture enriched with meat extract.
  • the new medium has a slightly reduced content due to the content of thioglucolate, so that in particular in connection with an oxygen-free protection containing nitrogen or carbon dioxide gas atmosphere, anaerobic bacteria are particularly protected.
  • the addition of meat extract causes a weak supply of nutrients, which obviously favors the persistence of the pathogens, but without growth and initiate an increase.
  • agar serves as a gel-forming agent in order to give the mixture the necessary strength. Quantities of 2-10 g / l are useful for this purpose, 5 g / 1 are preferred.
  • Sodium thioglucolate and sodium glycerol phosphate in turn serve on the one hand to make the mixture weakly reducing, which, as stated above, favors the anaerobic pathogens in particular, and on the other hand the pH value of the mixture of approx. 7.3 + 0, which is important for the viability of the bacteria, 1 to hold or adjust.
  • the mixture according to the invention is excellently suitable for the production of prefabricated transport containers which, if appropriate, are filled with sterile filling of the mixture into the usual transport tubes and vials and with a moisture-proof closure in a protective gas atmosphere and storage at normal temperatures between 6 and 20 ° C are still fully usable after 6-12 months.
  • the invention can be any suitable material. Depending on the intended purpose, the invention can be any suitable material.
  • Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen.
  • Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen.
  • antimicrobial additives such as vancomycin, kanamycin, nystatin or trimethoprim.
  • compositions according to the invention were compared against a commercial transport medium according to "Amies of the following composition: Agar no. 1 7.5
  • the preservative properties of the two transport media were compared after periods of 24, 48, 72 and 96 hours against a representative number of different gram-positive, gram-negative and anaerobic bacteria.
  • the results are summarized in the following table, where the results represent the mean values from a number of n samples.
  • cultures the pathogen in physiological saline solution are brought to a concentration of 5-10,000 germs / ml and 0.1 ml of this reading is sprayed onto the agar surface of the transport bottle.
  • the vials are each sealed with a rubber membrane and filled with a protective gas atmosphere for anaerobic bacteria.
  • 0.1 ml of the mixture is removed from agar plates which are suitable for the microorganism in question, spread out and incubated at 37 °.
  • the shelf life of the pathogens in the transport medium is assessed semi-quantitatively by evaluating the number of colonies in the classes: abundant (+++), moderate (++), isolated (+) and no germ growth (-).

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention concerns a new transport medium for microorganisms, in particular Gram-negative, Gram-positive and anaerobic bacteria, the medium optionally being contained, in ready-to-use form, in sterile sealed transport tubes or bottles. The transport medium consists of 2-10 g of agar, 1-3 of meat extract, 0.1-1 g of sodium chloride, 0.1-0.5 g of potassium chloride, 0.1-0.5 g of calcium chloride, 0.1-0.5 g of magnesium chloride, 0.5-2 g of sodium thioglucolate and 2-10 g of sodium glycerine phosphate, made up to 1,000 g with water, and the mixture has a pH of 7.3 ± 0.1 at 25 °C

Description

Transportmedium für Mikroorganismen  Transport medium for microorganisms
Die vorliegende Erfindung betrifft ein neues Transportmedium für Mikroorganismen, insbesondere gramnegative, grampositive und anaerobe Bakterien. The present invention relates to a new transport medium for microorganisms, in particular gram-negative, gram-positive and anaerobic bacteria.
Die exakte Diagnose einer Infektionskrankheit und die Auswahl dre richtigen therapeutischen Mittel hängt in vielen Fällen von eine Bestimmung der Erreger und dem Ermitteln der gegen sie wirksamen Antibiotica ab. Der gezielte Einsatz eines gegen den jeweiligen Erreger besonders wirksamen Heilmittels wird heute von der medizinischen Wissenschaft angestrebt, da so der Patient am wenigstens belastet und der Heilerfolg am besten sichergestellt wird. Die zunehmende Resistenzentwicklung von Bakterien gegen vor allem häufig eingesetzte Breitbandantibiotica zwingt in vielen Fällen sogar zu dieser Maßnahme. The exact diagnosis of an infectious disease and the selection of the right therapeutic agent in many cases depends on the determination of the pathogen and the determination of the antibiotics effective against it. The targeted use of a particularly effective remedy against the respective pathogen is sought by medical science today, since this is the least stressful for the patient and the best way to ensure the healing success. The increasing development of resistance of bacteria to broadly used broad-spectrum antibiotics, in many cases, even forces this measure.
Da die meisten ärztlichen Praxen für bakteriologische Untersuchungen entweder nicht ausgerüstet sind oder aus Rationalitätsgründen die Untersuchungen in Speziallabors durchführen lassen, ist es notwendig, die vom Patienten entnommenen Proben in das Labor zu überführen. Die Qualität der bakteriologischen Untersuchung wird dabei ganz entscheidend von der Qualität des Materials beeinflußt, das dem Labor zur Untersuchung bereitgestellt wird. Neben Fehlern bei der Probeentnahme hat sich insbesondere der Transport des Untersuchungsmaterials als eine erhebliche Since most medical practices are either not equipped for bacteriological examinations or, for reasons of rationality, have the examinations carried out in special laboratories, it is necessary to transfer the samples taken from the patient to the laboratory. The quality of the bacteriological examination is decisively influenced by the quality of the material that is made available to the laboratory for the examination. In addition to errors in sampling, the transport of the test material in particular has proven to be significant
Schwachstelle erwiesen. Außer bei sehr kurzen Transportzeiten müssen die Erreger daher durch ein geeignetes Transportmedium gegen eine Austrocknung und Oxidationsschädigung geschützt werden. Üblicherweise wird die Austrocknung durch Einbetten in ein geeignetes halbfestes Agarmedium und die Oxidationsschädigung durch ein Stickstoff- oder Kohlendioxid-haltiges Schutzgas erreicht. Bei spielhaft sei auf die Basisarbeiten von Stuart, Can. J. Pub. Vulnerability proven. Except for very short transport times, the pathogens must therefore be protected against drying out and oxidation damage using a suitable transport medium. Drying out is usually achieved by embedding in a suitable semi-solid agar medium and oxidation damage by means of a protective gas containing nitrogen or carbon dioxide. at be playful on the basics of Stuart, Can. J. Pub.
Health. 47, 114 (1956) und Amies, Can. J. Pub. Health 58, 296 (1967) verwiesen. Um dem Anwender die Handhabung zu vereinfachen, sind entsprechende mit einem Transportmedium und gegebenenfalls Schutzgas gefüllte Röhrchen oder Fläschchen von einer größeren Zahl von Firmen in den Handel gebracht worden. Health. 47, 114 (1956) and Amies, Can. J. Pub. Health 58, 296 (1967). In order to simplify handling for the user, corresponding tubes or vials filled with a transport medium and, if appropriate, protective gas have been brought onto the market by a large number of companies.
Die bekannten Transportmedien haben sich bei normaler Transportzeit von etwa 12-24 Std. und für aerobe und weniger empfindliche anaerobe Bakterien als durchaus brauchbar erwiesen. Bei längeren Transportzeiten von 48-72 Std. wie sie bei ungünstigen Verkehrsverhältnissen oder an Wochenenden vorkommen können und für The known transport media have proven to be quite useful for a normal transport time of about 12-24 hours and for aerobic and less sensitive anaerobic bacteria. With longer transport times of 48-72 hours as they can occur in unfavorable traffic conditions or on weekends and for
empfindliche anaerobe Bakterien lassen diese Transportmedien jedoch noch zu wünschen übrig, da die Keime unter diesen Bedingunge in unterschiedlichem Maße geschädigt werden, so daß beim anschließenden Anzüchten die Bakterienkultur für die ursprüngliche Erregerpopulation nicht mehr repräsentativ ist. However, sensitive anaerobic bacteria leave much to be desired in these transport media, since the germs are damaged to different degrees under these conditions, so that subsequent culture, the bacterial culture is no longer representative of the original pathogen population.
Es bestand daher die Aufgabe, neue Transportmedien zu finden, in denen besonders empfindliche Erreger längere Zeit in einem vermehrungsfähigen Zustand gehalten werden können. The task was therefore to find new transport media in which particularly sensitive pathogens can be kept in a reproductive state for a long time.
Diese Aufgabe wird durch die in den Ansprüchen näher gekennzeichneten Maßnahmen gelöst. This object is achieved by the measures characterized in more detail in the claims.
Das erfindungsgemäße Mittel weist in der Zusammensetzung starke Ähnlichkeiten mit dem bekannten Transportmedium von Amies auf, welches ebenfalls Agar, Natrium-, Kalium-, Calcium- und Magnesiumchlorid sowie Natriumthioglucolat enthält, jedoch ist der- Natriumphosphatpuffer von Amies durch einen Natriumglycerinphosphatpuffer ersetzt und zusätzlich die Mischung mit Fleischextrakt angereichert. The composition of the agent according to the invention has strong similarities to the known transport medium from Amies, which also contains agar, sodium, potassium, calcium and magnesium chloride and sodium thioglucolate, but the sodium phosphate buffer from Amies is replaced by a sodium glycerol phosphate buffer and additionally the mixture enriched with meat extract.
Das neue Medium wirkt aufgrund- des Gehaltes an Thioglucolat schwach reduziered, so daß insbesondere im Zusammenhang mit einer Sauerstoff-freien Stickstoff- oder Kohlendioxid-haltigen Schutz gasatmosphäre anaerobe Bakterien besonders geschützt werden. Im Gegensatz zu den bisherigen Transportmedien, bei denen das Medium selbst keine Nährstoffe enthielt, und diese nur gegebenenfalls in geringer Menge mit der Probe eingebracht wurden, bewirkt der Zusatz von Fleischextrakt ein schwaches Nährstoffangebot, welches offensichtlich das Persistieren der Erreger begünstigt, ohne jedoch ein Wachstum und damit eine Vermehrung zu initiieren. The new medium has a slightly reduced content due to the content of thioglucolate, so that in particular in connection with an oxygen-free protection containing nitrogen or carbon dioxide gas atmosphere, anaerobic bacteria are particularly protected. In contrast to the previous transport media, in which the medium itself did not contain any nutrients and these were only introduced in small quantities with the sample, the addition of meat extract causes a weak supply of nutrients, which obviously favors the persistence of the pathogens, but without growth and initiate an increase.
Agar dient in der erfindungsgemäßen Rezeptur als gelbildendes Mittel, um der Mischung die nötige Festigkeit zu verleihen. Mengen von 2-10 g/l sind für diesen Zweck sinnvoll, 5 g/1 werden bevorzugt. In the formulation according to the invention, agar serves as a gel-forming agent in order to give the mixture the necessary strength. Quantities of 2-10 g / l are useful for this purpose, 5 g / 1 are preferred.
Für die Zugabe des Fleischextraktes hat sich eine Menge von 2 g/l bewährt, Mengen von 1-3 g scheinen ebenfalls brauchbar zu sein. For the addition of the meat extract, a quantity of 2 g / l has proven itself, quantities of 1-3 g also seem to be useful.
Natrium-, Kalium-, Calcium- und Magnesiumchlorid werden der Sodium, potassium, calcium and magnesium chloride become the
Mischung zugesetzt, um den Ionenbedarf der zu transportiereridén Erreger einerseits zu decken und andererseits zusammen mit den. übrigen Inhaltsstoffen eine isotonische Zusammensetzung der Mixture added to meet the ion requirements of the pathogens to be transported on the one hand and on the other hand together with the. other ingredients an isotonic composition of the
Mischung zu gewährleisten. Mengen von 0,1-1,0 g/l haben sich für diesen Zweck bewährt, 0,1-0,5 g/l wurden bevorzugt. To ensure mixing. Quantities of 0.1-1.0 g / l have proven themselves for this purpose, 0.1-0.5 g / l were preferred.
Natriumthioglucolat und Natriumglycerinphosphat wiederum dienen einerseits dazu die Mischung schwach reduzierend zu machen, was wie oben gesagt insbesondere die anaeroben Erreger begünstigt, und andererseits dazu den für die Lebensfähigkeit der Bakterien wichtigen pH-Wert der Mischung im Optimum von ca. 7,3 + 0,1 zu halten bzw. einzustellen. Sodium thioglucolate and sodium glycerol phosphate in turn serve on the one hand to make the mixture weakly reducing, which, as stated above, favors the anaerobic pathogens in particular, and on the other hand the pH value of the mixture of approx. 7.3 + 0, which is important for the viability of the bacteria, 1 to hold or adjust.
Die erfindungsgemäße Mischung eignet sich hervorragend zur Herstellung von vorgefertigten Transportbehältern, die bei steriler Abfüllung der Mischung in die üblichen Transportröhren und Fläschchen und einem feuchtigkeitsdichten Verschluß, gegebenenfalls unter Schutzgasatmosphäre, und einer Lagerung bei üblichen Temperaturen zwischen 6 und 20°C ohne weiteres nach 6-12 Monaten noch voll gebrauchsfähig sind. The mixture according to the invention is excellently suitable for the production of prefabricated transport containers which, if appropriate, are filled with sterile filling of the mixture into the usual transport tubes and vials and with a moisture-proof closure in a protective gas atmosphere and storage at normal temperatures between 6 and 20 ° C are still fully usable after 6-12 months.
Je nach dem beabsichtigten Zweck kann der erfindungsgemäßen Depending on the intended purpose, the invention can
Mischung noch ein. Redoxindikator zugesetzt werden, wie Methylenblau oder Resazurin, der durch seine Verfärbung anzeigt, ob die Schutzgasatmosphäre, gegebenenfalls mit Sauerstoff vermischt ist. Soweit nur bestimmte Erreger untersucht werden sollen, kann durch einen Zusatz von selektiv wirkenden antimikrobiellen Zusätzen, wie Vancomycin, Kanamycin, Nystatin oder Trimethoprim das Wachstum der nicht-interessierenden Erreger, welche gegebenenfalls durch eine falsche Probeentnahme als Kontamination eingeschleppt werden, verhindert werden. Mix one more. Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen. To the extent that only certain pathogens are to be investigated, the growth of non-interested pathogens, which may be introduced as contamination by incorrect sampling, can be prevented by adding selectively acting antimicrobial additives, such as vancomycin, kanamycin, nystatin or trimethoprim.
In längeren Versuchsreihen hat sich folgende Zusammensetzung, als besonders vorteilhaft erwiesen: The following composition has proven to be particularly advantageous in longer test series:
Agar No . 1 5,00 g Agar no. 1 5.00 g
Fleischextrakt (LAB LEMCO) 2,00 g Meat extract (LAB LEMCO) 2.00 g
Natriumchlorid 0,50 g  Sodium chloride 0.50 g
Kaliumchlorid 0,20 g  Potassium chloride 0.20 g
Calciumchlorid 0,10 g  Calcium chloride 0.10 g
Magnesiumchlorid 0,10 g  Magnesium chloride 0.10 g
Natriumthioglycolat 1,00 g  Sodium thioglycolate 1.00 g
Natriumglycerinphosphat 10,00 g  Sodium glycerin phosphate 10.00 g
Wasser ad 1000, 00 g  Water ad 1000, 00 g
pH 7 , 3 ( 25 °C ) pH 7.3 (25 ° C)
Um die Vorteile der erfindungsgemäßen Zusammensetzungen zu zeigen wurde diese gegen ein handelsübliches Transportmedium nach "Amies der folgenden Zusammensetzung verglichen: Agar No . 1 7,5 In order to show the advantages of the compositions according to the invention, these were compared against a commercial transport medium according to "Amies of the following composition: Agar no. 1 7.5
Natriumchlorid 3,0  Sodium chloride 3.0
Kaliumchlorid 0,2  Potassium chloride 0.2
Calciumchlorid 0,1  Calcium chloride 0.1
Magnesiumchlorid 0,1  Magnesium chloride 0.1
Natriumthioglycolat 1,0  Sodium thioglycolate 1.0
NaH2PO4/Na2HPO4 1,4 NaH 2 PO 4 / Na 2 HPO 4 1.4
Wasser ad 1000  Water ad 1000
pH 7,3 (25°C) pH 7.3 (25 ° C)
Die konservierenden Eigenschaften der beiden Transportmedien wurden jeweils nach Zeiträumen von 24, 48, 72 und 96 Stunden gegenüber einer repräsentativen Zahl von verschiedensten grampositiven, gramnegativen und anaeroben Bakterien verglichen. Die Ergebnisse sind in der folgenden Tabelle zusammengefaßt, wobei di Ergebnisse jeweils die Mittelwerte aus einer Zahl von n Probjen darstellen. Für die Untersuchungszwecke werden jeweils Kulturen, der Erreger in physiologischher Kochsalzlösung auf eine Konzentration von 5-10.000 Keimen/ml gebracht und 0,1 ml dieser Lesung auf die Agaroberflache des Transportfläschchens aufgespritzt. Die Fläschchen sind jeweils mit einer Gummimembran verschlossen und für anaerobe Bakterien mit einer Schutzgasatmosphäre gefüllt. Zu den angegebenen Zeiten wird jeweils 0,1 ml der Mischung entnommen auf Agarplatten, welche für den betreffenden Mikroorganismus geeignet sind, ausgestrichen und bei 37° bebrütet. Die Haltbarkeit der Erreger im Transportmedium wird semiquantitativ durch Auswertung der Kolonienzahl in den Klassen: reichlich (+++), mäßig (++), vereinzelt (+) und kein Keimwachstum (-), beurteilt.
Figure imgf000008_0001
The preservative properties of the two transport media were compared after periods of 24, 48, 72 and 96 hours against a representative number of different gram-positive, gram-negative and anaerobic bacteria. The results are summarized in the following table, where the results represent the mean values from a number of n samples. For the purposes of the investigation, cultures, the pathogen in physiological saline solution are brought to a concentration of 5-10,000 germs / ml and 0.1 ml of this reading is sprayed onto the agar surface of the transport bottle. The vials are each sealed with a rubber membrane and filled with a protective gas atmosphere for anaerobic bacteria. At the times indicated, 0.1 ml of the mixture is removed from agar plates which are suitable for the microorganism in question, spread out and incubated at 37 °. The shelf life of the pathogens in the transport medium is assessed semi-quantitatively by evaluating the number of colonies in the classes: abundant (+++), moderate (++), isolated (+) and no germ growth (-).
Figure imgf000008_0001

Claims

P a t e n t a n s p r ü c h e Patent claims
1. Transportmedium für Mikroorganismen, bestehend aus 1. Transport medium for microorganisms consisting of
2-10 g Agar  2-10 g agar
1-3 g Fleischextrakt  1-3 g meat extract
0,1-1 g Natriumchlorid  0.1-1 g sodium chloride
0,1-0,5 g Kaliumchlorid  0.1-0.5 g potassium chloride
0,1-0,5 g Calciumchlorid  0.1-0.5 g calcium chloride
0,1-0,5 g Magnesiumchlorid  0.1-0.5 g magnesium chloride
0,5-2 g Natriumthioglucolat  0.5-2 g sodium thioglucolate
2-10 g Natriumglycerinphospat  2-10 g sodium glycerol phosphate
Wasser ad 1.000 g  Water ad 1,000 g
pH der Mischung 7,3 + 0,1 bei 25°C.  pH of the mixture 7.3 + 0.1 at 25 ° C.
2. Transportmedium gemäß Anspruch 1, bestehend aus 2. Transport medium according to claim 1, consisting of
5,00 g Agar  5.00 g agar
2,00 g Fleischextrakt  2.00 g meat extract
0,50 g Natriumchlorid  0.50 g sodium chloride
0,20 g Kaliumchlorid  0.20 g potassium chloride
0,10 g Calciumchlorid  0.10 g calcium chloride
0,10 g Magnesiumchlorid  0.10 g magnesium chloride
1,00 g Natriumthioglucolat  1.00 g sodium thioglucolate
10,0 g Natriumglycerinphospat  10.0 g sodium glycerol phosphate
Wasser ad 1.000 g  Water ad 1,000 g
pH der Mischung 7,3.  pH of the mixture 7.3.
3. Transportmedium gemäß Anspruch 1 oder 2, dadurch gekennzeichnet, daß es in gebrauchsfertiger Form in steril verschlossenen Transportröhrchen oder Transportfläschchen abgefüllt ist. 3. Transport medium according to claim 1 or 2, characterized in that it is filled in ready-to-use form in sterile sealed transport tubes or transport bottles.
4. Transportmedium gemäß Anspruch 3, dadurch gekennzeichnet, daß der Behälter eine Schutzgasatmosphäre aus Stickstoff oder Kohlendioxid enthält. 4. Transport medium according to claim 3, characterized in that the container contains a protective gas atmosphere made of nitrogen or carbon dioxide.
5. Transportagar gemäß einem der Ansprüche 1-4, dadurch gekennzeichnet, daß zusätzlich ein Oxidationsindikator oder ein selektiv wirkendes Antibioticum enthalten ist. 5. Transport agar according to one of claims 1-4, characterized in that an oxidation indicator or a selectively acting antibiotic is additionally contained.
PCT/EP1989/001003 1988-04-13 1989-08-25 Transport medium for microorganisms WO1991002786A1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0786663A1 (en) * 1996-01-26 1997-07-30 Kyowa Medex Co., Ltd. Method for storing liquid diagnostic reagents
US5783382A (en) * 1996-01-26 1998-07-21 Kyowa Medex Co., Ltd. Method for storing liquid diagnostic reagents

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