WO1991002786A1 - Milieu de transport pour micro-organismes - Google Patents
Milieu de transport pour micro-organismes Download PDFInfo
- Publication number
- WO1991002786A1 WO1991002786A1 PCT/EP1989/001003 EP8901003W WO9102786A1 WO 1991002786 A1 WO1991002786 A1 WO 1991002786A1 EP 8901003 W EP8901003 W EP 8901003W WO 9102786 A1 WO9102786 A1 WO 9102786A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sodium
- chloride
- transport
- transport medium
- agar
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Definitions
- the present invention relates to a new transport medium for microorganisms, in particular gram-negative, gram-positive and anaerobic bacteria.
- the known transport media have proven to be quite useful for a normal transport time of about 12-24 hours and for aerobic and less sensitive anaerobic bacteria. With longer transport times of 48-72 hours as they can occur in unfavorable traffic conditions or on weekends and for
- the task was therefore to find new transport media in which particularly sensitive pathogens can be kept in a reproductive state for a long time.
- composition of the agent according to the invention has strong similarities to the known transport medium from Amies, which also contains agar, sodium, potassium, calcium and magnesium chloride and sodium thioglucolate, but the sodium phosphate buffer from Amies is replaced by a sodium glycerol phosphate buffer and additionally the mixture enriched with meat extract.
- the new medium has a slightly reduced content due to the content of thioglucolate, so that in particular in connection with an oxygen-free protection containing nitrogen or carbon dioxide gas atmosphere, anaerobic bacteria are particularly protected.
- the addition of meat extract causes a weak supply of nutrients, which obviously favors the persistence of the pathogens, but without growth and initiate an increase.
- agar serves as a gel-forming agent in order to give the mixture the necessary strength. Quantities of 2-10 g / l are useful for this purpose, 5 g / 1 are preferred.
- Sodium thioglucolate and sodium glycerol phosphate in turn serve on the one hand to make the mixture weakly reducing, which, as stated above, favors the anaerobic pathogens in particular, and on the other hand the pH value of the mixture of approx. 7.3 + 0, which is important for the viability of the bacteria, 1 to hold or adjust.
- the mixture according to the invention is excellently suitable for the production of prefabricated transport containers which, if appropriate, are filled with sterile filling of the mixture into the usual transport tubes and vials and with a moisture-proof closure in a protective gas atmosphere and storage at normal temperatures between 6 and 20 ° C are still fully usable after 6-12 months.
- the invention can be any suitable material. Depending on the intended purpose, the invention can be any suitable material.
- Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen.
- Redox indicator can be added, such as methylene blue or resazurin, which shows through its discoloration whether the protective gas atmosphere is optionally mixed with oxygen.
- antimicrobial additives such as vancomycin, kanamycin, nystatin or trimethoprim.
- compositions according to the invention were compared against a commercial transport medium according to "Amies of the following composition: Agar no. 1 7.5
- the preservative properties of the two transport media were compared after periods of 24, 48, 72 and 96 hours against a representative number of different gram-positive, gram-negative and anaerobic bacteria.
- the results are summarized in the following table, where the results represent the mean values from a number of n samples.
- cultures the pathogen in physiological saline solution are brought to a concentration of 5-10,000 germs / ml and 0.1 ml of this reading is sprayed onto the agar surface of the transport bottle.
- the vials are each sealed with a rubber membrane and filled with a protective gas atmosphere for anaerobic bacteria.
- 0.1 ml of the mixture is removed from agar plates which are suitable for the microorganism in question, spread out and incubated at 37 °.
- the shelf life of the pathogens in the transport medium is assessed semi-quantitatively by evaluating the number of colonies in the classes: abundant (+++), moderate (++), isolated (+) and no germ growth (-).
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
La présente invention concerne un nouveau milieu de transport pour les micro-organismes, en particulier les bactéries Gram négatives, Gram positives et anaréobies, qui est placé éventuellement dans des tubes ou des bouteilles fermés et stériles prêts à l'emploi et de petite dimension. Le milieu de transport se compose de 2 à 10 g d'agar, de 1 à 3 g d'extrait de viande, de 0,1 à 1 g de chlorure de sodium, de 0,1 à 0,5 g de chlorure de potassium, de 0,1 à 0,5 g de chlorure de calcium, de 0,1 à 0,5 g de chlorure de magnésium, de 0,5 à 2 g de thioglycolate de sodium, de 2 à 10 g de glycérophosphate de sodium et d'un complément d'eau à 1000 g, et le pH du mélange est de 7,3 ± 0,1 à 25 °C.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19883812194 DE3812194A1 (de) | 1988-04-13 | 1988-04-13 | Transportmedium fuer mikroorganismen |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1991002786A1 true WO1991002786A1 (fr) | 1991-03-07 |
Family
ID=6351840
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP1989/001003 WO1991002786A1 (fr) | 1988-04-13 | 1989-08-25 | Milieu de transport pour micro-organismes |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE3812194A1 (fr) |
WO (1) | WO1991002786A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0786663A1 (fr) * | 1996-01-26 | 1997-07-30 | Kyowa Medex Co., Ltd. | Procédé pour le stockage de réactifs liquides de diagnostic |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0184019A1 (fr) * | 1984-11-09 | 1986-06-11 | Hitachi, Ltd. | Bactérie anaérobe thermophile, productrice d'une alpha-amylase thermostable, alpha-amylase thermostable et leur procédé de préparation |
EP0255124A2 (fr) * | 1986-07-29 | 1988-02-03 | Hitachi, Ltd. | Glucoamylase thermostable, méthode pour la production de glucose en utilisant celle-ci, et installation de production |
EP0275901A2 (fr) * | 1987-01-17 | 1988-07-27 | Hoechst Aktiengesellschaft | Utilisation de la gamma-glutamyl-transpeptidase |
-
1988
- 1988-04-13 DE DE19883812194 patent/DE3812194A1/de active Granted
-
1989
- 1989-08-25 WO PCT/EP1989/001003 patent/WO1991002786A1/fr unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0184019A1 (fr) * | 1984-11-09 | 1986-06-11 | Hitachi, Ltd. | Bactérie anaérobe thermophile, productrice d'une alpha-amylase thermostable, alpha-amylase thermostable et leur procédé de préparation |
EP0255124A2 (fr) * | 1986-07-29 | 1988-02-03 | Hitachi, Ltd. | Glucoamylase thermostable, méthode pour la production de glucose en utilisant celle-ci, et installation de production |
EP0275901A2 (fr) * | 1987-01-17 | 1988-07-27 | Hoechst Aktiengesellschaft | Utilisation de la gamma-glutamyl-transpeptidase |
Non-Patent Citations (1)
Title |
---|
DIALOG INFORMATION SERVICES, File 351, WORLD PATENT INDEX 81-90, Dialog Accession No. 89-316683/44, KLIETMANN W., "Agar Medium to Transport Microorganisms, Esp. Anaerobic Bacteria - Contg. Meat Extract, Salts, Reducing Agent and Sodium Glycerine Phosphate, Providing Long Term Viability"; & DE,A,3 812 194, 26-10-89, (Basic). * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0786663A1 (fr) * | 1996-01-26 | 1997-07-30 | Kyowa Medex Co., Ltd. | Procédé pour le stockage de réactifs liquides de diagnostic |
US5783382A (en) * | 1996-01-26 | 1998-07-21 | Kyowa Medex Co., Ltd. | Method for storing liquid diagnostic reagents |
Also Published As
Publication number | Publication date |
---|---|
DE3812194A1 (de) | 1989-10-26 |
DE3812194C2 (fr) | 1990-02-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE19936428B4 (de) | Eisenionen enthaltendes Bakterizid | |
Podbielski et al. | Growth inhibitory activity of gutta-percha points containing root canal medications on common endodontic bacterial pathogens as determined by an optimized quantitative in vitro assay | |
Kinniment et al. | The effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system | |
Jones | The effect of temperature, solids content and pH on the survival of salmonellas in cattle slurry | |
DD268481A5 (de) | Pruefmittel und verfahren zur bestimmung von antibiotika in milch und dabei zu benutzender, neuer stamm von streptococcus thermophilus | |
EP2103308A1 (fr) | Procédé de fabrication d'un mélange de bactériophages et leur utilisation dans le traitement de staphylocoques résistants aux antibiotiques | |
DE2657150A1 (de) | Verfahren und vorrichtung zum nachweis von mikroorganismen | |
DE2615362A1 (de) | Verfahren und vorrichtung zum transportieren und aufbewahren von anaerobischen mikroorganismen | |
BRPI0721035A2 (pt) | Soluções de armazenamento de meios cromatográficos e de equipamento cromatográfico e uso das mesmas. | |
DE3205018A1 (de) | Bifidobakterien vermehrendes mittel | |
DE3812194C2 (fr) | ||
Marshall et al. | The bacteriological and clinical assessment of a new preparation for the treatment of otitis externa in dogs and cats | |
DE2829316A1 (de) | Verfahren zum nachweis und zur untersuchung von zellulaerer aktivitaet und mittel zur durchfuehrung des verfahrens | |
Kronman et al. | Microbiologic evaluation of poly-HEMA root canal filling material | |
EP0165905A1 (fr) | Méthode d'essai diagnostique pour la détection de mélanges de bactéries oraux pathogéniques | |
CN108186491A (zh) | 一种含竹醋液的漱口水及其制备方法 | |
Karlovic et al. | Antibacterial activity of halothane, eucalyptol and orange oil | |
AKSU et al. | The possibilities for the use of commercial starter cultures in pastırma production | |
Broisman et al. | Antimicrobial effects of N2 in vitro | |
EP0569623A1 (fr) | Lyophilisat d'un milieu de culture contenant des micro-organismes à germes propageables | |
DE2212573C3 (de) | Prüfmittel zur halbquantitativen Bestimmung der in einer Flüssigkeit enthaltenen Mikroorganismen | |
Setiarso et al. | Production standardization of hand sanitizer spray based on ethanol, isopropanol, and bioethanol to prevent the COVID-19 transmission | |
CN109673669A (zh) | 一种纳米微乳碘的制备方法及其应用 | |
EP1633857B1 (fr) | Securisation hygienique du procede de pulverisation de dosage a plusieurs chambres et utilisation probiotique pour l'application de micro-organismes viables | |
Eisenstark et al. | Electron Micrographs of X-Ray—treated Escherichia coli Cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AU DK FI HU JP SU US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE FR GB IT LU NL SE |