CS196370B2 - Fodder admixture - Google Patents

Description

The invention relates to a stabilized vitamin additive, in particular for feeding animals. The properties of the product are particularly advantageous in terms of stability, absorption, accumulation, use and also the digestibility of vitamins, in particular vitamins A, D, E and K.

Use of vitamin solutions which contain fat-soluble vitamins and which are converted to "water-soluble". emulsification, increased in the area of animal feeding. This is due, among other things, to the automation of feeding, which is the result of enlarged stable units. Additional requirements for vitamins increased due to increased production levels (burden) and increased feed utilization. Supplementary vitamin A feeding has become bold and is in most cases easiest to administer separately in the form of liquid feed along with drinking water.

Orally administrable emulsions containing vitamins A, D, E (and sometimes K) or mixtures thereof are preferably prepared by incorporating the oily vitamin raw material together with the emulsifier under intensive stirring into a base or carrier, usually water. ;

When comparing the water-soluble products thus obtained with respect to their use, only the absorbent capacity of the emulsified vitamin (eg, vitamin A acetate / paimltarium) is usually considered; but not the possible effect of the base.

It has now surprisingly been found, according to the invention, that when an alcoholic sugar or a mixture of alcoholic sugars is incorporated into the vitamin E emulsion base, it is possible to increase the microbiological resistance, stability, absorption, you. use and digestibility of the vitamin emulsion. The preferred addition of the alcoholic sugar is 5 to 30% dry matter, based on the total weight of the vitamin emulsion, with the preferred addition being 15% w / w.

It was found that. it is particularly preferred to add xylite as well as molasses obtained as a byproduct in the production of xylite from plants. material such as wood into the emulsion base.

Further features of the invention are set forth in the definition of the subject matter of the invention.

The above product obtained from birch wood is a tan, sweet liquid with a specific gravity of about 1.22 as a 50% aqueous solution. Its caloric value and degree of sweetness are the same as for glucose.

The by-product contains several alcohols. sugar and usually has a composition based on dry matter as follows:

'' Table 'I xylit arabit manit sorblt dulcit rhamnit' other degradation 'products

1.5—25% by weight

20-35% by weight.

15-25 wt% 5'-15 wt. % 5-15 wt.%

5—10% by weight

2-5% by weight

2—5; % w / w

In the present processes, the xylite yield was increased and the dry weight composition was increased. may fluctuate in the following. borders:

Table II xylite 6-18 wt.% Arabite 9-21 wt.% Mannitol 13-19 wt.% Sorbit 8-12 wt.% Dulcite 5-11 wt.% Rhamnit 4-6 wt.% Reducing.

sugars 8-15% by weight, other polyols 6-14% by weight

It is known in the literature that, for example, xylite · 'stimulates liver function and increases cellular' activity. '' '' 'Also' known 'microbes' in the mouth are unable to 'utilize xylity as an energy source and that in rumen fluid' (in in vitro), sugar alcohols are stored without decomposition for a long time (Poutiainen E, et al., 1976, Proc. Nutr. Soc. 294th Scientific Assembly, London, pp. 140-141 and PV 1723-77).

The invention is. clarified with. The following examples contain additives. which are particularly preferred. ’

Example 1

Microbiological comparison,

In the example. 1 'was' examined. .microbiological applicability of various basic additives. . vitamin emulsions. Xylite and a by-product were used as additives. from the production of xylite. a. used in the comparative tests. . sucrose. and a clean water test was also performed.

In the tests, a solution containing 15% dry matter of xylite was used. by-product or sucrose as well as a solution containing pure water, mixed, with the suspension. microbes, containing the following microbes:

Escherichia coli (Gram-negative rod bacteria) ..............

Staphylococcus epidemis (Gram-positive coccus)

Saccharnomyces ..carlsbergensis:. (yeast).

January night 'old' precultures were mixed to form. a mixed suspension that was diluted 1: 1000. . 1 ml of this suspension was pipetted into. 100 ml of each test. solution. and the 'solutions' were transferred to an incubation chamber whose temperature was 35 ° C.

The concentration of microbes in the solutions was monitored for one month by performing basic cultures. on. bacterial count-agar and saboraud-agar. The test results are shown in Table III. The figures in the table show the concentration of microbes at each time,. determined as cell count / ml.

• Table.III ':

Microbiological 'comparison of basic' activities for different 'vitamin' emulsions

Incubation period,. 'days. led product in the production of xy lithium. mixed suspension of bacteria. 'vylis. yeast cells. per cell count- to ' -garu. saboraud · / ml ml Basic ingredient sucrose mixed suspension rain, water mixed suspension . xylit mixed. suspension bakt. cells on count-agar -ml vylis. yeast. cells on saboraudr -gar  bul Wakt. cells on count-agar. -ml vylis. yeast; cells on saboraud-agar. ml bakt. cells 'on count-agar.'. ml vylis: yeast. cells '. . at, saboraud- -garu. 'ml. 1 21000 500 25 'QOO 3 000 22? 000 600 35 000 200 2 100 300 500 100 ALIGN! 300 200 4 '300 3 700 9 60 1260 000 170 110 90. 60 6.ООО '000 100 '000 14 -. 1 340 000 ' 180 120 ’ 120 200 54 '000 34 '000 41 . - - ' -, - - - - -

· '- - · 1 «β370 · s 6

From the test results it can be summarized that if. When comparing sucrose, xylite and distilled water, the sucrose solution 'proved to be' clearly. best substrate for microbial growth. Xylite and distilled: the water was equivalent to each other, which is a phenomenon that 'xylite' is a poor nutrient - microbes, but under test conditions - it did not seem. that xylit would hinder - microbial growth - to any greater extent. Apparently, the by-product obtained from the production of xylite contains components that inhibit the growth of bacteria, but not the growth of yeast. Thus, it can be summarized that the bacteria are benefiting from poor alcoholic sugars, i.e. 'at least as badly' as in pure water. . : '. '.

Example - l 'ad 2:

sugar - containing vitamin -. solution -with a therapeutic-formulation

In this test. - were compared - two vitamin. solutions - what they are - tend to become contaminated. The formulations used were an aqueous vitamin solution (ADE-Vimin / Astra) and a corresponding vitamin A, D, E solution containing an additional 15% solids of alcoholic sugars (byproduct from xylite production, Table 1). ), related. for the whole weight - of the solution. Contamination - a. expeimental methods were the same - as - in. ·, Example 1. The results are shown in the table. IV. '

Microbiological comparison of alcoholic

Table IV

A microscopic comparison of a vitamin solution containing an alcoholic sugar with a medicinal product

Vitamin solution.

baatf '·' ·· _A- D _-EV rMr _N > _{/ A t} ADE-roztdk, containing generally:

time. ,, - .5. ^{/ Asra} product at. 'production of xylite days. sm®iaá ^'' 8uspénsé mixed 'suspension

bacterial cells count-agar ml ·. ··. pressed yeast cells into. saboraud agar ml ' bacterial 'cells on count-agar' ml the squeezed 'yeast cells' on saboraud-agar mu 1 52,000,000 25 '6ÓŮŮ0Ů' 31000 700 2 750 000 •. - -40 000 - -. '' 0 10θ ^! . ·. ··. ·. : .- 9- · '. · - .190- -000- / · - - - 160 - 000 - ·.  '-XX. -— ' 14; • 'v' 240,000 ' '10' 000 --- \.  ·. - ·. > · 40 .. · '' '---------'; '. 82'000 ^; -

From the results, it is apparent that - - - - aqueous - ADE - Vlmin (R) is a much better substrate for microbial growth than the corresponding vitamin A, D, E-containing solution and alcoholic sugar. The vitamin solution containing alcoholic sugar does not grow microbes shortly after contamination. In one day, the bacterial content was reduced to less than 1/100 of the amount - after contamination, - that is, the vitamin 'solution. - Containing - - - Alcoholic sugar is very stable microbiologically.

The same kind of contamination test was also done using a periodic 'contamination process' with two other known vitamin products. The test results are shown in Table V.

The addition of alcoholic sugar makes the vitamin product very stable to microbes. The contamination disappeared in any case in one day. ·.

Table V

Microbial growth in. different vitamin products JDoba vitamin product days 'A-D-Vitamin Orion'. А- ^ Е-уКашЬ! Aster mixed suspension . mixed. suspense. bakt. cells . Extract cells bakt. cells vylis. cells on count-agar yeast. ' on on 'count-agar yeast. on : ml saboraud-agar, ml · .- saboraud-agar ml. mi, '. . . . 1 ' 9 <100  . '<100 900 90 000 ..  ·. ‘’ Ώ (konto- '' ' minace). 600 600 90 000 . . '2 000 000 ^: ... 3 .. . 150 . 550 20 '000: 2 000 000 9 <100 <100 200 000 200 000 10 3 000 2 000 250 000 250 000. 11 3 500 2 000 100 000 1 000 17 <100 <100 100 0.Q0. .. .... ·;,. 300 000. 18 (konto- minace) 3 000 000 700 000 2 000 00 600 000 19 Dec 1 500 000 25 '000 1 000 000 250 000 24 ' ^: - -' - . . .. _; '. ..,. 500'Ό0Ό ^? . 40 O00 25 (konto- minace) 3 000 000 90Ó 000 1000 000 .... 4 000 000 26 4 000 000 600 000 2 000 000 1,000,000 32 200 '' 000 20 000 ·· - 800,000 500 000. 49. <100 ' . <100. 400 '000 2 000 000 Table V .......... pokráčování ........... Time Vitamin product idní To-ό-νϋβιηίη Rohto .. 'A-D-E-Vitamin 15% sugar alcohols mixed suspension *, a laughable suspension. bakt. cells vylis. . kvass, cells bakt. cells . vylis. cells on cout-agar on saboraud-agar on cunt-agar on saboraud-agar ml ml ml . ml. . i _: 9 5 0.00 40 000 <100 '. < ¹⁰⁰  . , · . (konto-. minace) 800 2 000 <100 <100 3 <100 5 000 <100 <100 9 <100 . <100 <100 <100.  10 (konto- '' -! . minace) 2,000 2 000 2 000 . 2 0Ó0 11 <100 <100 <100 <100 17 <100 <100 <100. <100 ' 18 2 000 000 800 000 200 000 . 70 000 19 Dec • <100 300 <100 <100 24 <100 <100. <100 . <100 25 (konto- minace) 4,000'000 4 000 400 000 > 90,000 26 5 000 700 000 <100 <100 32 <100 <100 <100 <100 49 <100 <100 <100 <100

ижвжтго ŘPrXk ha rd. '. в

Stability of vitamins

In this test, the protection of vitamins was investigated at various temperatures when alcoholic sugar (a byproduct of xylite production, tensile I) was added to the vitamin emulsion base in an amount of 15% solids, calculated from the total weight of the emulsion. contained an emulsion of 30 Q00 MJ of vitamin A, 3,000 MJ of vitamin D3 and 30 mg of vitamine Έ n ^: a'ml. Base water was used, to which some alcohols were added and other necessary means were added. ··. The preparation was frozen (- <20 ° C) and kept at this temperature for 10 days, after which the preparation was thawed and kept at room temperature [- 420 ° te] for 53 days, after which the product was kept in a refrigerator at 4 ° C. Low: 4 ° C.

Samples were taken from the preparation during the freezing period (3 days), after storage at room temperature (-] - 20 ^° C) for 53 days, blanket, the product being stored for a total of 9 months. The samples were tested ^; women in their vitamins at every stage. The results are shown in Table VI.

Table VI ^ Preservation of vitamin emulsion at various temperatures .During time ° C Vitamin.E Vitamin A. Shadows · —20 = 36 (732Ό00 '53 days -4-20 34.7 31300

-'97. Network & Networks 4- 4 33,931460

From the test results it can be concluded that the vitamin content of the preparation is preserved for a long time under different temperature conditions.

Example 4,

Movement of radiolabeled vitamin A in the digestive tract

In these tests, the movement of radioactivity in the digestive tract was observed in Ά, D, E solutions.

A -A1. ( 'All trans) .1- ³ H (N), · means of three groups of five mice nonpathogenic. The results of the test are shown in Table VII, which shows the radioactivity detected in the gastrointestinal tract two hours after oral administration, calculated as a% of the amount administered. The basic additive of the vitamin emulsion was xylite and a byproduct from the production of xylite (Table I), and a test series was used in which pure water was used as a throw. Alcoholic sugar accounted for 15% of the dry matter, mass emidae.

Table VII

- Movement of radioactivity in the digestive tract (

part alive, organ xylit .radioactivity pure water led, product

stomach 3,404-0,24 * -4,644-0,12- * 5,884-0,20 small intestine - front part 6,504-0,26 6,264-0, ‘24 7, = 564-1.00 small intestine - back 2,864-0,08 3,124-0,16 2,624-0,16 • large intestine 2,764-0,02 3,164-0,12 3,68-40,36 • Total content 15.52 217.16 1’9,7-4

The xylity and by-product levels of the standard mean error.

The levels of xylity and byproduct in the stomach differ statistically significantly from those obtained with pure water. A significant difference between xylite and water is (Studt's t-test) P <0.05 and the corresponding value for the by-product is P <0.01, which is a statistically significant difference.

Values for other parts of the gastrointestinal tract do not differ / significantly from those obtained with water, i.e. significantly lower radioactivity was found in the stomach of the test when the base of the labeled vitamin emulsion contained an alcohol sugar, meaning radioactivity ceased either by absorption into organism, or with excrement. .

Example 1 a d 5

Excretion of radiolabelled vitamin A with excrement

In the test of Example 4, the transfer of isotopically labeled vitamin A to the excrements of the test animals was monitored.

Vitamin A was administered orally to a group of non-pathogenic mice as in Example 4 and excrement samples were collected daily for 30 days. The values of the rams are shown in the following table. samples of xxre- VIII.

Table VIII

Radioactivity of animal excrement samples time, days xylite by-product pure water

17.62 · 0.04 -

8,8613,20

0,06.0,12

Thus, the radioactivity of the xylite-containing emulsions and the byproduct from xylite production disappeared from the intestines (absorption area) more rapidly than from the pure water-based emulsions as shown in Example 4. However, no corresponding increase in excrement was found, rather the opposite as shown in Example 5.

Less than 1% of the administered dose was excreted in the urine. There were no significant statistical differences between groups.

Example 6

Absorption of tritium-labeled vitamin A into the body

From the foregoing examples, it can be expected that radioactivity could move more rapidly into the blood serum of the liver and other internal organs from xylite-based and by-product solutions. '

To confirm this, a single dose of 450 IU of Vitamin A (labeled tritium) in the form of a vitamin A, D, E solution was administered orally to non-pathogenic mice and the liver radioactivity was determined as% of the dose, 2 and 6 hours and 1, 2 , 3 and 7 days after administration. The results are shown in Table IX and Figure 1.

Table IX Blood serum radioactivity

time xylit side product clean water 2 hours 0.38 + 0.02 *** 0.29 + 0.01 0.29 + 0.02 6 hours 0.30 + 0.03 0.28 + 0.01 0.26 + 0.01 1 day 0.16 + 0.01 0.16 <+0.01 0.16 + 0.01 2 days 0.12 + 0.01 0.13 <+0.01 ' 0.13 + 0.01 3 days 0.08 <+0.01 0.09 <+0.01 0.08 <+0.01 7 days 0.06 <± 0.01 0.05 <+0.01 0.07 + 0.01

The value for xylite in the table after 2 hours differs statistically very significantly (P <0.001) from the corresponding value for water. The corresponding value for the by-product is dissent. For the other values, no significant differences could be detected.

Figure 1 shows the corresponding liver radioactivity results. The results in the figure are mean values + standard mean error. The results obtained with xylite after 2 and 6 hours differ statistically to a large extent from those obtained with pure water. The axis shows the time in days, the proper axis shows the% of the indicated dose.

(·) Means values for xylit.

Δ means values for by-product,

I · I means values for * “ ^J pure water.

The amount of radioactivity read for kidneys, brain and heart was less than 1%. There were no statistically significant differences between groups.

It can be concluded from Examples 4 to 6 that sugar alcohols, in particular xylite, as well as a by-product formed during the production of xylite from plant material, stimulate the absorption of vitamins as well as their intestines by accumulation into the body and their use in the body.

SUBJECT OF THE INVENTION

Claims (4)

A feed additive comprising fat-soluble and water-soluble vitamins emulsified in water, in particular an emulsion comprising vitamins A, D, E and K, characterized in that it additionally contains an alcohol sugar or a mixture of alcohol sugars in an amount of 5 to 30 wt. %, preferably 15 wt. %, Calculated as the dry weight of the total weight of the product, to increase stability, absorption, accumulation, recovery and digestibility. emulsified vitamins. 2. The additive according to claim 1, wherein the alcoholic sugar is xylite. 3. The additive according to claim 1, characterized in that the mixture of alcoholic sugars is a by-product obtained in the manufacture of xylite from a vegetable material. such as wood, and contains, in particular, the alcoholic sugars xylite, arabite, sorbitol, mannitol, dulcite and rhamnit. 4. The additive according to claim 1, characterized in that it is in liquid or dry form. Severograafia, n. P.,. Race 7, Most