SU668573A3 - Emulsifier of fodder vitamin preparations - Google Patents

Description

(54) EMULGATOR FODDER VITAMIN PREPARATIONS

Mannit15-25

Sorbitol5-15

Dulcit5-15

Ramnit5-15

Other components 2-5

Decomposition products 2-5.

In later methods, xylitol regeneration was improved, and the composition, calculated on the basis of dry weight, may vary within the following limits, by weight. %:

Xylitol 6-18

lrabit9-21

Mannit13-19

Sorbitol. 8-12

Ramnite

Dulcit5-11

Restoring

sugar8-15

Other polyols 6-14.

Example 1. In example 1, the microbiological applicability of various basic additives in vitamin emulsion was investigated. Xylitol and by-products of xylitol production were used as additives, sucrose was used in comparative experiments, and experiments with pure water were also performed.

Microbiological effect of various vitamin emulsion base supplements

In these experiments, a microbial suspension containing the following microbes was injected into a solution containing 15% xylitol, a byproduct of the product or sucrose as dry matter, as well as a solution containing distilled water: SschericJiia Coii

(gram negative wand)

(gram-positive piQfthjjtoCoCtv epitJenu tel kok) Sciccbaromyces sagas in Run; 5 (yeast).

The overcultures grown overnight were mixed by forming a mixed suspension which was diluted 1: 100. 1 ml of this suspension was transferred with a pipette into 10 ml of each test solution and the resulting solutions were transferred to an incubation chamber whose temperature was maintained.

The concentration of microbes was determined after one month and was determined on a bacterial counting

25 agar and aborartrf agar. Data obtained results are shown in table 1.

Table 1

From these test results, it can be concluded that when comparing the microbiological action of sucrose, xylitol, a by-product of xylitol production and distilled water, the sucrose solution turned out to be the best substrate for microbial growth. Xylitol and distilled water are equivalent in effect, which proves that xylitol is a poor nutrient for microbes, but under the conditions of experience it appears that xylitol does not inhibit microbial growth to any degree. The by-product obtained in the production of xylitol contains components that inhibit the growth of bacteria, but not yeast. From this microbiological comparison

it follows that bacteria also reproduce poorly in sugar alcohols, as in pure water.

Example 2. In this experiment, two vitamin solutions were compared in terms of their ability to be exposed to microbes. The preparations used were aqueous solutions of vitamin A-D-E (Vimm.®. Astra) and the corresponding solution of vitamins A-D-E, containing an additional 15% sugar alcohols (a by-product of xylitol production) in the form of dry matter on the total weight of the solution. The method of infection and the experimental conditions were the same as in example 1. The data obtained

5 results are shown in Table 2.

Table 2 of vitamin solution containing alcoholic sugar, with medication

It follows from the above data that the A-D-E Vfmin® aqueous solution is a significantly better plant substrate for microbes than the corresponding solutions of vitamins A, D, and E containing sugar alcohol. In a sugar alcohol-containing vitamin solution, microbial growth ceases quickly after infection. After one day, the bacterial content decreased by less than 0.01 after infection, i.e. The vitamin solution containing sugar alcohol is sufficiently microbially stable.

The same infection experiment was performed using a periodic infection technique with two additional known comparative vitamin products.

Results are shown in Table 3. As follows from the above data, the addition of alcoholic sugar makes the vitamin product sufficiently resistant to germs.

Microbial growth in various vitamin products

DOE

100

100

Example Z. in this experiment investigated the stability of vitamins at different temperatures with the addition of 15% sugar alcohol (a by-product of xylitol production) to vigamin emulsion in the form of a dry process calculated on the total weight of the emulsions. U and 30 mg of vitamin E Per 1 ml. Distilled water was used as a base, to which sugar alcohol and other necessary agents were added.

5000

40000 100

100

90000

The drug was frozen to –20 ° C and held at this temperature for 10 days, after which it was thawed and kept at room temperature.

for 53 days; Then the product was placed in a refrigerator and kept there at. Samples were taken from the preparation 3 days after freezing. 53 days after storage

at room temperature and after being stored in a refrigerator for 9 months, they were tested for their vitamin content.

These test results are given in Table 4.

Vitamin emulsion stability at different temperatures

On the basis of these test results, it can be concluded that the vitamin content in the preparation is maintained for a long time under various temperature CONDITIONS.

Example 4. In these experiments, the change labeled with radioactive and the change of radioactivity in the alimentary tract

The values shown in Table 5 are the average SEM values

The radioactivity of xylitol and by-product in the stomach is significantly different from the values obtained using pure water. The difference between xylitol and water is, 05, and the corresponding value for the by-product, 01, i.e. this difference is statically significant. The values of radioactivity in other parts of the alimentary tract are not noticeably different from those obtained using water, i.e. significantly less radioactivity was detected in the stomach of the tested LIVES when the base of the labeled vitamin emulsion contained sugar alcohol, i.e. radioactivity disappears either due to absorption in the body, or with excrement.

Example 5. In example 4, the transition is observed isotope-labeled vitamin B in the excrement of the test animal. The vitamin preparation was administered orally to groups consisting of five ZRG mice and samples of excrement were collected daily for 30 days.

substances in the alimentary tract were investigated in solutions of vitamins A, D, E using tritiated labeled yitamin L, A (weight-trans / 1 -H / N), using three groups of five S F F special non-pathogenic . The results of this test are given in c. table.5.

Table5

The results of the determination of radioactivity in the obtained excrement samples are given in Table 6.

Table of Radioactivity of Excrement Samples in Test Animals

As shown in Example 4, the radioactivity of the emulsions, whose bases contain xylitol and a by-product of xylitol production, disappears from the intestines (absorption area) more quickly than with pure water emulsions as the basis. However, the corresponding increase was not found in feces, and even the opposite result is observed, as shown in Example 5. Less than 1% of the applied amount is excreted in urine. No significant statistical differences were found between the groups.

eleven

Example 6. Based on the previous examples, one would expect that the radioactivity should penetrate faster into the blood serum, liver and other internal organs from solutions containing xylitol and a by-product of its production, from solutions based on water.

To establish this fact, the serum single activity was orally administered to SPF mice.

In this table, the values for xylitol after 2 hours are statistically very different (, 001) from the corresponding value for water. Corresponding values for the by-product are inconsistent. As for other values, no significant differences were found.

Radioactivity found in the kidneys, brain and heart was less than 1%. There was no significant statistical difference between the groups.

The IDs of Examples 4-6 can be concluded that sugar alcohols, especially xylitol, as well as the by-product obtained in the production of xylitol from plant material, stimulate the absorption of vitamins from the bale, as well as their accumulation and consumption by the body.

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A single dose of 4503 (7 vitamin A (labeled with tritium) in the form of a solution of vitamin A, D, E and the radioactivity of blood serum in the liver was determined as a percentage of the dosages, after 2 and bch, and also through 1, 2, 3 and 7 days after 5 administration.

Data obtained results are shown in table.7.

Table7

Claims (2)

1. Tkachev I., Preparation and rational use of feed., Krasnodar, Krasnodar book of publishing, 1962, pp. 171-173. 2. USSR patent application No. 2463649 / 30-15, cl. A 23 K 1/00, 1976.