WO1990014090A1 - SHORT THERAPEUTIC dsRNA OF DEFINED STRUCTURE - Google Patents

SHORT THERAPEUTIC dsRNA OF DEFINED STRUCTURE Download PDF

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Publication number
WO1990014090A1
WO1990014090A1 PCT/US1989/002172 US8902172W WO9014090A1 WO 1990014090 A1 WO1990014090 A1 WO 1990014090A1 US 8902172 W US8902172 W US 8902172W WO 9014090 A1 WO9014090 A1 WO 9014090A1
Authority
WO
WIPO (PCT)
Prior art keywords
dsrna
human
therapeutically
short
therapy
Prior art date
Application number
PCT/US1989/002172
Other languages
English (en)
French (fr)
Inventor
David H. Gillespie
William A. Carter
Original Assignee
Hem Research, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hem Research, Inc. filed Critical Hem Research, Inc.
Priority to JP1503637A priority Critical patent/JPH04507083A/ja
Priority to EP19890906635 priority patent/EP0473576A4/en
Priority to PCT/US1989/002172 priority patent/WO1990014090A1/en
Publication of WO1990014090A1 publication Critical patent/WO1990014090A1/en
Priority to DK911889A priority patent/DK188991D0/da
Priority to NO91914529A priority patent/NO914529L/no

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
PCT/US1989/002172 1989-05-19 1989-05-19 SHORT THERAPEUTIC dsRNA OF DEFINED STRUCTURE WO1990014090A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP1503637A JPH04507083A (ja) 1989-05-19 1989-05-19 規定された構造の短い治療用ｄｓＲＮＡ
EP19890906635 EP0473576A4 (en) 1989-05-19 1989-05-19 Short therapeutic dsrna of defined structure
PCT/US1989/002172 WO1990014090A1 (en) 1989-05-19 1989-05-19 SHORT THERAPEUTIC dsRNA OF DEFINED STRUCTURE
DK911889A DK188991D0 (da) 1989-05-19 1991-11-19 Terapeutisk virksom kort, dobbeltstrenget rna
NO91914529A NO914529L (no) 1989-05-19 1991-11-19 Kort terapeutisk dsrna med definert struktur

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1989/002172 WO1990014090A1 (en) 1989-05-19 1989-05-19 SHORT THERAPEUTIC dsRNA OF DEFINED STRUCTURE

Publications (1)

Publication Number Publication Date
WO1990014090A1 true WO1990014090A1 (en) 1990-11-29

Family

ID=22215026

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/002172 WO1990014090A1 (en) 1989-05-19 1989-05-19 SHORT THERAPEUTIC dsRNA OF DEFINED STRUCTURE

Country Status (5)

Country Link
EP (1) EP0473576A4 (no)
JP (1) JPH04507083A (no)
DK (1) DK188991D0 (no)
NO (1) NO914529L (no)
WO (1) WO1990014090A1 (no)

Cited By (65)

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WO1993012229A1 (fr) * 1991-12-18 1993-06-24 Cis Bio International Procede de preparation d'arn double-brin, et ses applications
WO2000001846A2 (en) * 1998-07-03 2000-01-13 Devgen N.V. Characterisation of gene function using double stranded rna inhibition
WO2000068374A1 (en) * 1999-05-10 2000-11-16 Syngenta Participations Ag Regulation of viral gene expression
WO2003070918A3 (en) * 2002-02-20 2004-07-08 Ribozyme Pharm Inc Rna interference by modified short interfering nucleic acid
GB2397818A (en) * 2002-02-20 2004-08-04 Sirna Therapeutics Inc Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
NL1026335C2 (nl) * 2004-06-04 2005-12-06 Univ Delft Tech Werkwijze voor het maken van een dubbelstrengs polyribonucleotidenvolgorde met overhangend uiteinde, alsmede een werkwijze voor het vormen van een dubbelstrengs polynucleotidenconstruct en een toepassing.
US7005423B1 (en) 1999-07-02 2006-02-28 Devgen Nv Characterization of gene function using double stranded RNA inhibition
US7022828B2 (en) 2001-04-05 2006-04-04 Sirna Theraputics, Inc. siRNA treatment of diseases or conditions related to levels of IKK-gamma
US7176304B2 (en) 2002-02-20 2007-02-13 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7491805B2 (en) 2001-05-18 2009-02-17 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7560438B2 (en) 1997-12-23 2009-07-14 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US7659389B2 (en) 2001-05-18 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MYC and/or MYB gene expression using short interfering nucleic acid (siNA)
US7659390B2 (en) 2002-02-20 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
US7662951B2 (en) 2000-08-30 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
US7662952B2 (en) 2002-02-20 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US7667029B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7667030B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
US7678897B2 (en) 2002-02-20 2010-03-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
US7691999B2 (en) 2002-02-20 2010-04-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US7700760B2 (en) 2002-02-20 2010-04-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US7744900B2 (en) 1999-04-08 2010-06-29 Novartis Vaccines And Diagnostics, Inc. Enhancement of the immune response for vaccine and gene therapy applications
US7795422B2 (en) 2002-02-20 2010-09-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA)
US7833992B2 (en) 2001-05-18 2010-11-16 Merck Sharpe & Dohme Conjugates and compositions for cellular delivery
US7858769B2 (en) 2004-02-10 2010-12-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using multifunctional short interfering nucleic acid (multifunctional siNA)
US7858625B2 (en) 2001-05-18 2010-12-28 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7888325B2 (en) 1999-01-28 2011-02-15 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US7893248B2 (en) 2002-02-20 2011-02-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
US7897752B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of telomerase gene expression using short interfering nucleic acid (siNA)
US7897753B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of XIAP gene expression using short interfering nucleic acid (siNA)
US7897757B2 (en) 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of protein tyrosine phosphatase-1B (PTP-1B) gene expression using short interfering nucleic acid (siNA)
US7910725B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7910724B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA)
US7915400B2 (en) 2002-02-20 2011-03-29 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) gene expression using short interfering nucleic acid (siNA)
US7923547B2 (en) 2002-09-05 2011-04-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923549B2 (en) 2002-02-20 2011-04-12 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7928220B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of stromal cell-derived factor-1 (SDF-1) gene expression using short interfering nucleic acid (siNA)
US7928218B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US7928219B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (SINA)
US7935812B2 (en) 2002-02-20 2011-05-03 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA)
US7943757B2 (en) 2002-02-20 2011-05-17 Mcswiggen James RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA)
US7977472B2 (en) 2002-02-20 2011-07-12 Leonid Beigelman RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA)
US7985853B2 (en) 2002-02-20 2011-07-26 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) gene expression using short interfering nucleic acid (siNA)
US7989612B2 (en) 2002-02-20 2011-08-02 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8008472B2 (en) 2001-05-29 2011-08-30 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of human immunodeficiency virus (HIV) gene expression using short interfering nucleic acid (siNA)
US8008473B2 (en) 2002-02-20 2011-08-30 Mcswiggen James RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA)
US8013143B2 (en) 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of CXCR4 gene expression using short interfering nucleic acid (siNA)
US8017761B2 (en) 2001-05-18 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of Stearoyl-CoA desaturase (SCD) gene expression using short interfering nucelic acid (siNA)
US8053419B2 (en) 1998-03-20 2011-11-08 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US8067575B2 (en) 2002-02-20 2011-11-29 Merck, Sharp & Dohme Corp. RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA)
US8148604B2 (en) 2004-10-21 2012-04-03 Venganza Inc. Methods and materials for conferring resistance to pests and pathogens of plants
US8168774B2 (en) 1998-03-20 2012-05-01 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
US8183217B2 (en) 1999-08-13 2012-05-22 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US8258288B2 (en) 2002-02-20 2012-09-04 Sirna Therapeutics, Inc. RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA)
US8273866B2 (en) 2002-02-20 2012-09-25 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US8329890B2 (en) 2002-08-05 2012-12-11 University Of Iowa Research Foundation SiRNA-mediated gene silencing
US8481710B2 (en) 2002-08-05 2013-07-09 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use thereof
US8524879B2 (en) 2002-08-05 2013-09-03 University Of Iowa Research Foundation RNA interference suppresion of neurodegenerative diseases and methods of use thereof
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
US9051566B2 (en) 2001-01-31 2015-06-09 Alnylam Pharmaceuticals, Inc. Post-transcriptional gene silencing using expressed double stranded RNA
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9260471B2 (en) 2010-10-29 2016-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US9441239B2 (en) 1998-04-08 2016-09-13 Commonwealth Scientific & Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference

Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3444043A (en) * 1966-09-30 1969-05-13 Univ Illinois Interference with biologically active rna formation
US3660564A (en) * 1968-12-24 1972-05-02 Takeda Chemical Industries Ltd Interferon induction
US3679654A (en) * 1968-08-20 1972-07-25 Roland Maes Interferon or viral inhibitor protein induction
SU425940A1 (ru) * 1968-09-17 1974-04-30 Н. С. Сидорова, С. Е. Бреслер, Н. А. Зейтленок , Л. М. Вильнер Способ получения синтетическогодвухнитчатого полирибонуклеотидногокомплекса
US3819482A (en) * 1972-05-08 1974-06-25 J Semancik Method of preparing high yields of double-stranded ribonucleic acid
US4018916A (en) * 1975-01-27 1977-04-19 Beecham Group Limited Antiviral ionic complexes of double-standard RNA
US4024241A (en) * 1974-09-27 1977-05-17 The United States Of America As Represented By The Secretary Of Health, Education And Welfare Nuclease-resistant hydrophilic complex of polyriboinosinic-polyribocytidylic acid
US4024222A (en) * 1973-10-30 1977-05-17 The Johns Hopkins University Nucleic acid complexes
US4124702A (en) * 1971-07-06 1978-11-07 Merck & Co., Inc. Polynucleotides active as inducers of interferon production in living animal cells
US4262090A (en) * 1979-06-04 1981-04-14 Cetus Corporation Interferon production
US4313938A (en) * 1978-07-28 1982-02-02 The Green Cross Corporation Interferon inducer and method of preparing same
US4349538A (en) * 1979-12-07 1982-09-14 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Nuclease-resistant hydrophilic complex of polyriboinosinic-polyribocytidylic acid
US4388306A (en) * 1980-06-04 1983-06-14 Merck & Co., Inc. Pharmaceutical composition comprising modified polyriboinosinic-polyribocytidylic acid, for induction of interferon in primates
US4400375A (en) * 1981-11-23 1983-08-23 Eli Lilly And Company Tobramycin-double stranded RNA complex suitable for inducing interferon
US4767701A (en) * 1985-08-29 1988-08-30 Berol Kemi Ab Poly I:C covalently bonded to polymer for diagnostic purposes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1820588A (en) * 1987-07-17 1989-01-19 Hem Research, Inc. Double-stranded rna correction of abnormalities in circulating immune complexes and monocyte function
IE66830B1 (en) * 1987-08-12 1996-02-07 Hem Res Inc Topically active compositions of double-stranded RNAs
IE72103B1 (en) * 1987-08-12 1997-03-12 Hem Res Inc Promotion of host defense by systemic dsRNA treatment
DE3853755T2 (de) * 1987-09-04 1995-12-14 Hem Pharma Corp Diagnose von Mangelzuständen doppelsträngiger RNS.
US4963532A (en) * 1987-11-25 1990-10-16 Hem Research, Inc. dsRNA-based prevention of viral escape

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3444043A (en) * 1966-09-30 1969-05-13 Univ Illinois Interference with biologically active rna formation
US3679654A (en) * 1968-08-20 1972-07-25 Roland Maes Interferon or viral inhibitor protein induction
SU425940A1 (ru) * 1968-09-17 1974-04-30 Н. С. Сидорова, С. Е. Бреслер, Н. А. Зейтленок , Л. М. Вильнер Способ получения синтетическогодвухнитчатого полирибонуклеотидногокомплекса
US3660564A (en) * 1968-12-24 1972-05-02 Takeda Chemical Industries Ltd Interferon induction
US4124702A (en) * 1971-07-06 1978-11-07 Merck & Co., Inc. Polynucleotides active as inducers of interferon production in living animal cells
US3819482A (en) * 1972-05-08 1974-06-25 J Semancik Method of preparing high yields of double-stranded ribonucleic acid
US4024222A (en) * 1973-10-30 1977-05-17 The Johns Hopkins University Nucleic acid complexes
US4130641A (en) * 1973-10-30 1978-12-19 Ts O Paul O P Induction of interferon production by modified nucleic acid complexes
US4024241A (en) * 1974-09-27 1977-05-17 The United States Of America As Represented By The Secretary Of Health, Education And Welfare Nuclease-resistant hydrophilic complex of polyriboinosinic-polyribocytidylic acid
US4018916A (en) * 1975-01-27 1977-04-19 Beecham Group Limited Antiviral ionic complexes of double-standard RNA
US4313938A (en) * 1978-07-28 1982-02-02 The Green Cross Corporation Interferon inducer and method of preparing same
US4262090A (en) * 1979-06-04 1981-04-14 Cetus Corporation Interferon production
US4349538A (en) * 1979-12-07 1982-09-14 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Nuclease-resistant hydrophilic complex of polyriboinosinic-polyribocytidylic acid
US4388306A (en) * 1980-06-04 1983-06-14 Merck & Co., Inc. Pharmaceutical composition comprising modified polyriboinosinic-polyribocytidylic acid, for induction of interferon in primates
US4400375A (en) * 1981-11-23 1983-08-23 Eli Lilly And Company Tobramycin-double stranded RNA complex suitable for inducing interferon
US4767701A (en) * 1985-08-29 1988-08-30 Berol Kemi Ab Poly I:C covalently bonded to polymer for diagnostic purposes

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
"Augmenting Agents in Cancer Therapy", Issued 1981, Raven Press, (New York, USA), W.A. CARTER et al. "Poly IC with Mismatched Bases, Prospects for Cancer Therapy", see pp. 177-183. *
Acta Virology, Vol. 14, Issued 1970, I. ROSZTOCY et al., "Enhancement of Interferon Synthesis by Polyinosinic-Polycytidylic Acid in L Cells Pretreated with Interferon", see pp. 398-400. *
Cancer Research, Vol. 46, Issued April 1986 (Bethesda, Maryland, USA), M.S. CHAPEKAR et al., "Potentiation of the Cytocidal Effect of Human Immune Interferon by Different Synthetic Double-Stranded RNAs in the Refractory Human Colon Carcinoma Cell Line BE", see pp. 1698-1702. *
CHEMICAL ABSTRACTS, Vol. 107, Issued 1987 (Columbus, Ohio, USA), D.C. MONTEFIORI et al., "Antiviral activity of mismatched double-stranded RNA against human immunodeficiency virus in vitro", see Abstract Number 51430a on p.21. *
CHEMICAL ABSTRACTS, Vol. 108, Issued 1988 (Columbus, Ohio, USA), H.R. HUBBELL et al., "Augmented antitumor effect of combined human natural interferon-alpha and mismatched double-stranded RNA treatment against human malignant melanoma xenograft", see Abstract Number 4453w on p 434. *
Journal of Biological Chemistry, Vol. 254, Issued 25 October 1979 (Easton, Pennsylvania, USA), M.A. MINKS et al., "Structural requirements of double-stranded RNA for the activation of 2',5'-oligo(A) polymerase and protein kinase of interferon-treated HeLa cells.", see pp. 10180-10183. *
Molecular Pharmacology, Vol. 15, Issued 1979 (New York, New York, USA), J.A. O'MALLEY et al., "Polyinosinic Acid-Polycytidylic Acid and Its Mismatched Analogues: Differential Effects on Human Cell Function", see pp. 165-173. *
Nature, Vol. 297, Issued 03 June 1982, (London, England), S.L. LIN et al. "Sensitivity and resistance of human tumour cells to interferon and rIn.rCn", see pp. 417-419. *
Proceedings of the National Academy of Science USA, Issued 1967 (Washington, D.C. USA), A.K. FIELD et al., "Inducers of Interferon and Host Resistance, II. Multistranded Synthetic Polynucleotide Complexes", see pp. 1004-1010. *
See also references of EP0473576A4 *

Cited By (120)

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FR2685346A1 (fr) * 1991-12-18 1993-06-25 Cis Bio Int Procede de preparation d'arn double-brin, et ses applications.
US5795715A (en) * 1991-12-18 1998-08-18 Cis Bio International Process for preparing double-stranded RNA, and its applications
WO1993012229A1 (fr) * 1991-12-18 1993-06-24 Cis Bio International Procede de preparation d'arn double-brin, et ses applications
US7622633B2 (en) 1997-12-23 2009-11-24 Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US7560438B2 (en) 1997-12-23 2009-07-14 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US9102939B2 (en) 1997-12-23 2015-08-11 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US8053419B2 (en) 1998-03-20 2011-11-08 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US8168774B2 (en) 1998-03-20 2012-05-01 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
US9441239B2 (en) 1998-04-08 2016-09-13 Commonwealth Scientific & Industrial Research Organisation Methods and means for obtaining modified phenotypes
GB2362885A (en) * 1998-07-03 2001-12-05 Devgen Nv Characterisation of gene function using double stranded (ds) RNA inhibition
GB2362885B (en) * 1998-07-03 2002-07-31 Devgen Nv Expression vector capable of producing double stranded RNA inhibition
GB2349885B (en) * 1998-07-03 2003-01-29 Devgen Nv Characterisation of gene function using double stranded RNA inhibition
US8114980B2 (en) 1998-07-03 2012-02-14 Devgen Nv Characterisation of gene function using double stranded RNA inhibition
EP1197567A3 (en) * 1998-07-03 2003-01-02 Devgen NV Characterisation of gene function using double stranded RNA inhibition
WO2000001846A2 (en) * 1998-07-03 2000-01-13 Devgen N.V. Characterisation of gene function using double stranded rna inhibition
CZ304897B6 (cs) * 1998-07-03 2015-01-07 Devgen N. V. Způsob vnesení dvouřetězcové RNA nebo DNA schopné produkovat dvouřetězcovou RNA a neterapeutické použití bakteriální nebo kvasinkové buňky
GB2349885A (en) * 1998-07-03 2000-11-15 Devgen Nv Characterisation of gene function using double stranded RNA inhibition
WO2000001846A3 (en) * 1998-07-03 2000-06-15 Devgen Nv Characterisation of gene function using double stranded rna inhibition
EP1197567A2 (en) 1998-07-03 2002-04-17 Devgen NV Characterisation of gene function using double stranded RNA inhibition
EP2045336A3 (en) * 1998-07-03 2009-06-10 Devgen NV Characterisation of gene function using double stranded RNA inhibition
JP2009112311A (ja) * 1998-07-03 2009-05-28 Devgen Nv 二本鎖ｒｎａ阻害を用いる遺伝子機能の評価
US8148345B2 (en) 1999-01-28 2012-04-03 Georgia Health Sciences University Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US7888325B2 (en) 1999-01-28 2011-02-15 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US7744900B2 (en) 1999-04-08 2010-06-29 Novartis Vaccines And Diagnostics, Inc. Enhancement of the immune response for vaccine and gene therapy applications
EP1801215A3 (en) * 1999-05-10 2008-12-17 Syngeta Participations AG Regulation of viral gene expression
WO2000068374A1 (en) * 1999-05-10 2000-11-16 Syngenta Participations Ag Regulation of viral gene expression
EP1801215A2 (en) * 1999-05-10 2007-06-27 Syngeta Participations AG Regulation of viral gene expression
US7005423B1 (en) 1999-07-02 2006-02-28 Devgen Nv Characterization of gene function using double stranded RNA inhibition
US8183217B2 (en) 1999-08-13 2012-05-22 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US10190127B2 (en) 1999-08-13 2019-01-29 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US8017765B2 (en) 2000-08-30 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated treatment of alzheimer's disease using short interfering nucleic acid (siNA)
US7662951B2 (en) 2000-08-30 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
US9051566B2 (en) 2001-01-31 2015-06-09 Alnylam Pharmaceuticals, Inc. Post-transcriptional gene silencing using expressed double stranded RNA
US7022828B2 (en) 2001-04-05 2006-04-04 Sirna Theraputics, Inc. siRNA treatment of diseases or conditions related to levels of IKK-gamma
US7491805B2 (en) 2001-05-18 2009-02-17 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US8017761B2 (en) 2001-05-18 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of Stearoyl-CoA desaturase (SCD) gene expression using short interfering nucelic acid (siNA)
US7833992B2 (en) 2001-05-18 2010-11-16 Merck Sharpe & Dohme Conjugates and compositions for cellular delivery
US7858625B2 (en) 2001-05-18 2010-12-28 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7659389B2 (en) 2001-05-18 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MYC and/or MYB gene expression using short interfering nucleic acid (siNA)
US7964578B2 (en) 2001-05-18 2011-06-21 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US8008472B2 (en) 2001-05-29 2011-08-30 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of human immunodeficiency virus (HIV) gene expression using short interfering nucleic acid (siNA)
US7956178B2 (en) 2002-02-20 2011-06-07 Mcswiggen James RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US8273866B2 (en) 2002-02-20 2012-09-25 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US7897756B2 (en) 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US7897757B2 (en) 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of protein tyrosine phosphatase-1B (PTP-1B) gene expression using short interfering nucleic acid (siNA)
US7910725B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7910724B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA)
US7915400B2 (en) 2002-02-20 2011-03-29 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) gene expression using short interfering nucleic acid (siNA)
US10889815B2 (en) 2002-02-20 2021-01-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923549B2 (en) 2002-02-20 2011-04-12 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7928220B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of stromal cell-derived factor-1 (SDF-1) gene expression using short interfering nucleic acid (siNA)
US7928218B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US7928219B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (SINA)
US7935812B2 (en) 2002-02-20 2011-05-03 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA)
US7943757B2 (en) 2002-02-20 2011-05-17 Mcswiggen James RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA)
US7897752B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of telomerase gene expression using short interfering nucleic acid (siNA)
US10662428B2 (en) 2002-02-20 2020-05-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7897755B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
US7977472B2 (en) 2002-02-20 2011-07-12 Leonid Beigelman RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA)
US7985853B2 (en) 2002-02-20 2011-07-26 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) gene expression using short interfering nucleic acid (siNA)
US7989612B2 (en) 2002-02-20 2011-08-02 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7893248B2 (en) 2002-02-20 2011-02-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
US8008473B2 (en) 2002-02-20 2011-08-30 Mcswiggen James RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA)
US8013143B2 (en) 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of CXCR4 gene expression using short interfering nucleic acid (siNA)
US8013146B2 (en) 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
US10351852B2 (en) 2002-02-20 2019-07-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7858771B2 (en) 2002-02-20 2010-12-28 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
US7855284B2 (en) 2002-02-20 2010-12-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US8067575B2 (en) 2002-02-20 2011-11-29 Merck, Sharp & Dohme Corp. RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA)
US8076472B2 (en) 2002-02-20 2011-12-13 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
US7795422B2 (en) 2002-02-20 2010-09-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA)
WO2003070918A3 (en) * 2002-02-20 2004-07-08 Ribozyme Pharm Inc Rna interference by modified short interfering nucleic acid
US7700760B2 (en) 2002-02-20 2010-04-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US8153778B2 (en) 2002-02-20 2012-04-10 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US7691999B2 (en) 2002-02-20 2010-04-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US7678897B2 (en) 2002-02-20 2010-03-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
US8202979B2 (en) 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
US8258288B2 (en) 2002-02-20 2012-09-04 Sirna Therapeutics, Inc. RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA)
US7897753B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of XIAP gene expression using short interfering nucleic acid (siNA)
US10000754B2 (en) 2002-02-20 2018-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
GB2397818A (en) * 2002-02-20 2004-08-04 Sirna Therapeutics Inc Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
GB2397818B (en) * 2002-02-20 2005-03-09 Sirna Therapeutics Inc Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9957517B2 (en) 2002-02-20 2018-05-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9771588B2 (en) 2002-02-20 2017-09-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7667030B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
US9738899B2 (en) 2002-02-20 2017-08-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8846894B2 (en) 2002-02-20 2014-09-30 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7667029B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7662952B2 (en) 2002-02-20 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US7659390B2 (en) 2002-02-20 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
US7176304B2 (en) 2002-02-20 2007-02-13 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US9732344B2 (en) 2002-02-20 2017-08-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8481710B2 (en) 2002-08-05 2013-07-09 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use thereof
US9487779B2 (en) 2002-08-05 2016-11-08 University Of Iowa Research Foundation siRNA-mediated gene silencing
US9260716B2 (en) 2002-08-05 2016-02-16 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use thereof
US8779116B2 (en) 2002-08-05 2014-07-15 University Of Iowa Research Foundation SiRNA-mediated gene silencing
US8524879B2 (en) 2002-08-05 2013-09-03 University Of Iowa Research Foundation RNA interference suppresion of neurodegenerative diseases and methods of use thereof
US10072264B2 (en) 2002-08-05 2018-09-11 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use
US8329890B2 (en) 2002-08-05 2012-12-11 University Of Iowa Research Foundation SiRNA-mediated gene silencing
US7923547B2 (en) 2002-09-05 2011-04-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7956176B2 (en) 2002-09-05 2011-06-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7858769B2 (en) 2004-02-10 2010-12-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using multifunctional short interfering nucleic acid (multifunctional siNA)
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
WO2005118807A1 (en) * 2004-06-04 2005-12-15 Technische Universiteit Delft Process for creating a double-stranded polyribonucleotide sequence with terminal overhang, as well as a process for creating a double-stranded polynucleotide construct and an application
NL1026335C2 (nl) * 2004-06-04 2005-12-06 Univ Delft Tech Werkwijze voor het maken van een dubbelstrengs polyribonucleotidenvolgorde met overhangend uiteinde, alsmede een werkwijze voor het vormen van een dubbelstrengs polynucleotidenconstruct en een toepassing.
US9121034B2 (en) 2004-10-21 2015-09-01 Venganza Inc Methods and materials for conferring resistance to pests and pathogens of corn
US8148604B2 (en) 2004-10-21 2012-04-03 Venganza Inc. Methods and materials for conferring resistance to pests and pathogens of plants
US8461416B2 (en) 2004-10-21 2013-06-11 Venganza, Inc. Methods and materials for conferring resistance to pests and pathogens of plants
US8581039B2 (en) 2004-10-21 2013-11-12 Venganza, Inc. Methods and materials for conferring resistance to pests and pathogens of plants
US9970005B2 (en) 2010-10-29 2018-05-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US9260471B2 (en) 2010-10-29 2016-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US11193126B2 (en) 2010-10-29 2021-12-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)

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