WO1987004091A1 - Stimulation de l'activite enzymatique lors du nettoyage de lentilles de contact par l'utilisation de solutions hypotoniques - Google Patents

Stimulation de l'activite enzymatique lors du nettoyage de lentilles de contact par l'utilisation de solutions hypotoniques Download PDF

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Publication number
WO1987004091A1
WO1987004091A1 PCT/US1987/000045 US8700045W WO8704091A1 WO 1987004091 A1 WO1987004091 A1 WO 1987004091A1 US 8700045 W US8700045 W US 8700045W WO 8704091 A1 WO8704091 A1 WO 8704091A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
solution
lenses
contact lenses
cleaning
Prior art date
Application number
PCT/US1987/000045
Other languages
English (en)
Inventor
Stanley W. Huth
Sam W. Lam
Original Assignee
Allergan, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allergan, Inc. filed Critical Allergan, Inc.
Priority to AT87900942T priority Critical patent/ATE83179T1/de
Priority to DE8787900942T priority patent/DE3782981T2/de
Publication of WO1987004091A1 publication Critical patent/WO1987004091A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/0005—Other compounding ingredients characterised by their effect
    • C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only

Definitions

  • This invention relates to the potentiation of the enzymatic activity in cleaning contact lenses. More specifically, this invention is a method for potentiating the proteolytic activity of proteases in the cleaning of contact lenses by carrying out the cleaning in a hypotonic solution.
  • Contact lenses particularly those with a hydrophilic surface such as the hydrogel lenses and the hard, gas permeable lenses with a treated surface, encounter protein accretions during normal wear. It is beneficial, if not often times necessary, to remove these accretions in order to maintain visual acuity, prevent eye irritation and to prevent the development of giant papillary conjunctivitis.
  • This invention covers a method for enhancing the activity of an enzyme used in the cleaning of contact lenses, which method comprises carrying out the cleaning regime in a solution having an osmolality value up to about 275 milliosmoles/kilogram.
  • hypotonic solution to enhance enzyme activity for cleaning contact lenses is applicable to any proteolytic enzyme which may be used to remove protein from contact lenses.
  • Tonicity values may range from 0 up to about 275 mOsm/kg.
  • the enzyme itself will be active at tonicity values close to 0. but as a practical matter it is difficult to prepare such solutions because of the solutes normally present in diluents, including purified water. Tests have been carried out where the osmolality was as low as 6 mOsm/kg. A more preferred lower limit is about 50 mOsm/kg. which number allows for the addition of small amounts of salts, stabilizers, enzyme co-factors or other excipients which may be useful and beneficial to solution stability, enzyme activity or the like.
  • milliosmoles is approximately the top end of the range so far as enjoying substantially enhanced proteolytic activity is concerned. More preferably, the upper limit will be between 175 and 200 mOsm/kg.
  • tonicity value can be made with any number of excipients and constituents well known in the art. If an enzyme co-factor is critical or essential to the cleaning process, obviously its presence must be given primary consideration in adding materials to the formulation. Salts of any sort including salts which are necessary co-factors for enzymatic activity such as calcium should be accounted for in formulating these hypotonic solutions. The hypotonic value is determined, that is measured, after addition of the enzyme.
  • the ability of a given solution and enzyme to remove protein from a contact lens was determined by essentially the same procedure each time. Generically. the procedure was to take a contact lens and coat it with heat denatured lysozyme by placing the lens in a phosphate buffered saline solution to which was then added sufficient lysozyme to make a 0.1% solution by weight. The lysozyme was from egg white. These solutions were then heated for 30 minutes at about 95 C. The lenses were removed, cooled and rinsed with distilled water and viewed to determine what type of lysozyme accretion was on the lens at time zero.
  • Protein deposit classification as a means for quantifying the proteolytic activity of enzymes in removing absorbed protein from the lenses, a system was developed whereby the protein on the lenses was visually quantified before and after enzyme treatment.
  • the lens was wetted with distilled water, rubbed between the thumb and finger, then grasped by the edge with plastic tweezers and rinsed with distilled water again.
  • the convex side of the lens was viewed under a microscope at 100X magnif ication.
  • a film or deposit detected under these conditions was classified according to the percentage of the lens surface covered by the deposit.
  • the protein removal efficacy of an enzyme solution is expressed in terms of the percentage of the lens surface which has been cleaned. This number is derived by subtracting the percentage of the lens surface covered by the protein deposit from 100%.
  • a subtilisin enzyme containing solution was prepared as follows: cysteine hydrochloride monohydrate (1.001g), sodium borate dihydrate (1.911 g), sodium carbonate anhydrous (3.106g), polyethylene glycol 3350 (0.403g). tartaric acid (2.002g). S. carlsberg (0.041g).
  • Table I shows that greater cleaning was observed at 150 mOsm/kg than at either 326 or 420 mOsm/kg.
  • Hydrogel-type lenses (Hydrocurve II®. 55% water, sold by Barnes-Hind. Inc.) were treated with heat-denatured lysozyme as described above.
  • a subtilisin-A-containing solution was prepared (0.04 mg/ml of water without excipients. activity: 0.0012 Au/ml) in such a manner as to have pH values between approximately 5.0 and 10.0.
  • the osmolality value was then adjusted to 6. 133 and 264 mOsm/kg with NaCl for each of these solutions, each of the tonicity values being tested at pH 9.0.
  • Lenses were then soaked in these solutions (five in each) for 2 hours at room temperature, rinsed, and analyzed for percentage residual protein deposit as described above. Results are given in Table II.
  • subtilisin-A is an alkaline protease, having its greatest activity between pH 8 and 10, the greatest cleaning efficacy is observed in this pH range also.
  • Example 3 Lenses with lysozyme protein deposits covering at least 98% of the lens surface were cut in half, one half being soaked in one enzyme solution and the other in another enzyme solution in the first comparison (pancreatin data); whole lenses were used in the other studies.
  • the enzyme solutions were comprised of enzyme and excipients as indicated in Table III.
  • Lenses were bufilcon-A sold by Barnes-Hind, Inc. under the name Hydrocurve II®. Results from each of the several formulations are listed in the following table. The abbreviation DI is used for deionized water. Table I I I
  • a subtilisin enzyme tablet was dissolved in either 10ml of DI water or saline. Each enzyme tablet contained: 30mg N-acetylcysteine, 34mg sodiua carbonate, 7mg tartaric acid, 4mg polyethylene glycol 3350, 4mg subtillsin-A (subtilisin carlsberg from NOVO Industries of Denmark), and 50mg of lactose.
  • Hydrocurve II lenses (55% H 2 O) from Barnes-Hind were coated with denatured lysozyme as described in Example 1. Three lenses were soaked in the 95mOsm/kg solution and four lenses in each of the other two solutions. Soaking time was 3.5 hours. Total percent surface cleaned was determined as per Example 1. the results are given in Table IV.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Eyeglasses (AREA)

Abstract

Des solutions hypotoniques stimulent l'activité enzymatique lors du nettoyage protéolitique de lentilles de contact. La valeur osmolaire de la solution peut varier entre 0 et 275 mOsm/kg, mais se situera de préférence entre 100 et 200 mOsm/kg.
PCT/US1987/000045 1986-01-06 1987-01-05 Stimulation de l'activite enzymatique lors du nettoyage de lentilles de contact par l'utilisation de solutions hypotoniques WO1987004091A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AT87900942T ATE83179T1 (de) 1986-01-06 1987-01-05 Erhoehung von enzymatischer wirksamkeit bei der reinigung von kontaktlinsen durch verwendung von hypotonischen loesungen.
DE8787900942T DE3782981T2 (de) 1986-01-06 1987-01-05 Erhoehung von enzymatischer wirksamkeit bei der reinigung von kontaktlinsen durch verwendung von hypotonischen loesungen.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81651886A 1986-01-06 1986-01-06
US816,518 1986-01-06

Publications (1)

Publication Number Publication Date
WO1987004091A1 true WO1987004091A1 (fr) 1987-07-16

Family

ID=25220860

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1987/000045 WO1987004091A1 (fr) 1986-01-06 1987-01-05 Stimulation de l'activite enzymatique lors du nettoyage de lentilles de contact par l'utilisation de solutions hypotoniques

Country Status (6)

Country Link
EP (1) EP0252974B1 (fr)
AT (1) ATE83179T1 (fr)
AU (1) AU6898887A (fr)
DE (1) DE3782981T2 (fr)
HK (1) HK113995A (fr)
WO (1) WO1987004091A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0359574A2 (fr) * 1988-09-15 1990-03-21 Alcon Laboratories, Inc. Solutions aqueuses antimicrobiennes pour l'entretien des lentilles de contact
EP0384666A2 (fr) * 1989-02-21 1990-08-29 BAUSCH & LOMB INCORPORATED Méthode et composition pour le nettoyage et la désinfection de lentilles de contact

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5783532A (en) * 1993-06-17 1998-07-21 Allergan Enzyme compositions and methods for contact lens cleaning
IL109705A (en) * 1993-06-17 1998-07-15 Allergan Inc Enzyme compositions and methods for contact lens cleaning

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3240709A (en) * 1962-05-16 1966-03-15 Burton Parsons Chemicals Inc Method of cleansing contact lenses
US3910296A (en) * 1973-04-20 1975-10-07 Allergan Pharma Method of removing proteinaceous deposits from contact lenses
US4048122A (en) * 1976-01-23 1977-09-13 Barnes-Hind Pharmaceuticals, Inc. Cleaning agents for contact lenses
US4263054A (en) * 1979-02-23 1981-04-21 George D. Weaver Contact lens cleaning and rinsing method
US4285738A (en) * 1978-04-24 1981-08-25 Senju Pharmaceutical Co., Ltd. Cleaning composition for contact lenses
US4521254A (en) * 1981-02-09 1985-06-04 Anderson Ronald L Cleaning contact lenses with solution of bromelain and carboxypeptidase
US4613380A (en) * 1985-04-01 1986-09-23 Dow Corning Corporation Method for removing lipid deposits from contact lenses
US4626292A (en) * 1982-06-01 1986-12-02 Sherman Laboratories, Inc. Soft contact lens wetting and preservation method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2088581A (en) * 1980-10-02 1982-06-09 Smith & Nephew Associated Cie Papain and lactose containing tablet for cleaning contact lenses
GB2117534B (en) * 1982-03-31 1986-02-19 Smith & Nephew Ass Papain containing tablet for cleaning contact lenses
US4670178A (en) * 1985-09-09 1987-06-02 Allergan Pharmaceuticals, Inc. Method for the simultaneous cleaning and disinfecting of contact lenses

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3240709A (en) * 1962-05-16 1966-03-15 Burton Parsons Chemicals Inc Method of cleansing contact lenses
US3910296A (en) * 1973-04-20 1975-10-07 Allergan Pharma Method of removing proteinaceous deposits from contact lenses
US3910296B1 (fr) * 1973-04-20 1987-04-14
US4048122A (en) * 1976-01-23 1977-09-13 Barnes-Hind Pharmaceuticals, Inc. Cleaning agents for contact lenses
US4285738A (en) * 1978-04-24 1981-08-25 Senju Pharmaceutical Co., Ltd. Cleaning composition for contact lenses
US4263054A (en) * 1979-02-23 1981-04-21 George D. Weaver Contact lens cleaning and rinsing method
US4521254A (en) * 1981-02-09 1985-06-04 Anderson Ronald L Cleaning contact lenses with solution of bromelain and carboxypeptidase
US4626292A (en) * 1982-06-01 1986-12-02 Sherman Laboratories, Inc. Soft contact lens wetting and preservation method
US4613380A (en) * 1985-04-01 1986-09-23 Dow Corning Corporation Method for removing lipid deposits from contact lenses

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0252974A4 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0359574A2 (fr) * 1988-09-15 1990-03-21 Alcon Laboratories, Inc. Solutions aqueuses antimicrobiennes pour l'entretien des lentilles de contact
EP0359574A3 (fr) * 1988-09-15 1990-08-29 Alcon Laboratories, Inc. Solutions aqueuses antimicrobiennes pour l'entretien des lentilles de contact
EP0384666A2 (fr) * 1989-02-21 1990-08-29 BAUSCH & LOMB INCORPORATED Méthode et composition pour le nettoyage et la désinfection de lentilles de contact
EP0384666A3 (fr) * 1989-02-21 1991-01-23 BAUSCH & LOMB INCORPORATED Méthode et composition pour le nettoyage et la désinfection de lentilles de contact
US5096607A (en) * 1989-02-21 1992-03-17 Bausch & Lomb Incorporated Method for cleaning and disinfecting contact lenses

Also Published As

Publication number Publication date
AU6898887A (en) 1987-07-28
DE3782981T2 (de) 1993-04-08
ATE83179T1 (de) 1992-12-15
DE3782981D1 (de) 1993-01-21
EP0252974A4 (fr) 1988-05-03
HK113995A (en) 1995-07-21
EP0252974B1 (fr) 1992-12-09
EP0252974A1 (fr) 1988-01-20

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