EP0252974A4 - Enhancement of enzymatic activity in cleaning contact lenses by the use of hypotonic solutions. - Google Patents
Enhancement of enzymatic activity in cleaning contact lenses by the use of hypotonic solutions.Info
- Publication number
- EP0252974A4 EP0252974A4 EP19870900942 EP87900942A EP0252974A4 EP 0252974 A4 EP0252974 A4 EP 0252974A4 EP 19870900942 EP19870900942 EP 19870900942 EP 87900942 A EP87900942 A EP 87900942A EP 0252974 A4 EP0252974 A4 EP 0252974A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- enzyme
- solution
- lenses
- contact lenses
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
Definitions
- This invention relates to the potentiation of the enzymatic activity in cleaning contact lenses. More specifically, this invention is a method for potentiating the proteolytic activity of proteases in the cleaning of contact lenses by carrying out the cleaning in a hypotonic solution.
- Contact lenses particularly those with a hydrophilic surface such as the hydrogel lenses and the hard, gas permeable lenses with a treated surface, encounter protein accretions during normal wear. It is beneficial, if not often times necessary, to remove these accretions in order to maintain visual acuity, prevent eye irritation and to prevent the development of giant papillary conjunctivitis.
- This invention covers a method for enhancing the activity of an enzyme used in the cleaning of contact lenses, which method comprises carrying out the cleaning regime in a solution having an osmolality value up to about 275 milliosmoles/kilogram.
- hypotonic solution to enhance enzyme activity for cleaning contact lenses is applicable to any proteolytic enzyme which may be used to remove protein from contact lenses.
- Tonicity values may range from 0 up to about 275 mOsm/kg.
- the enzyme itself will be active at tonicity values close to 0. but as a practical matter it is difficult to prepare such solutions because of the solutes normally present in diluents, including purified water. Tests have been carried out where the osmolality was as low as 6 mOsm/kg. A more preferred lower limit is about 50 mOsm/kg. which number allows for the addition of small amounts of salts, stabilizers, enzyme co-factors or other excipients which may be useful and beneficial to solution stability, enzyme activity or the like.
- milliosmoles is approximately the top end of the range so far as enjoying substantially enhanced proteolytic activity is concerned. More preferably, the upper limit will be between 175 and 200 mOsm/kg.
- tonicity value can be made with any number of excipients and constituents well known in the art. If an enzyme co-factor is critical or essential to the cleaning process, obviously its presence must be given primary consideration in adding materials to the formulation. Salts of any sort including salts which are necessary co-factors for enzymatic activity such as calcium should be accounted for in formulating these hypotonic solutions. The hypotonic value is determined, that is measured, after addition of the enzyme.
- the ability of a given solution and enzyme to remove protein from a contact lens was determined by essentially the same procedure each time. Generically. the procedure was to take a contact lens and coat it with heat denatured lysozyme by placing the lens in a phosphate buffered saline solution to which was then added sufficient lysozyme to make a 0.1% solution by weight. The lysozyme was from egg white. These solutions were then heated for 30 minutes at about 95 C. The lenses were removed, cooled and rinsed with distilled water and viewed to determine what type of lysozyme accretion was on the lens at time zero.
- Protein deposit classification as a means for quantifying the proteolytic activity of enzymes in removing absorbed protein from the lenses, a system was developed whereby the protein on the lenses was visually quantified before and after enzyme treatment.
- the lens was wetted with distilled water, rubbed between the thumb and finger, then grasped by the edge with plastic tweezers and rinsed with distilled water again.
- the convex side of the lens was viewed under a microscope at 100X magnif ication.
- a film or deposit detected under these conditions was classified according to the percentage of the lens surface covered by the deposit.
- the protein removal efficacy of an enzyme solution is expressed in terms of the percentage of the lens surface which has been cleaned. This number is derived by subtracting the percentage of the lens surface covered by the protein deposit from 100%.
- a subtilisin enzyme containing solution was prepared as follows: cysteine hydrochloride monohydrate (1.001g), sodium borate dihydrate (1.911 g), sodium carbonate anhydrous (3.106g), polyethylene glycol 3350 (0.403g). tartaric acid (2.002g). S. carlsberg (0.041g).
- Table I shows that greater cleaning was observed at 150 mOsm/kg than at either 326 or 420 mOsm/kg.
- Hydrogel-type lenses (Hydrocurve II®. 55% water, sold by Barnes-Hind. Inc.) were treated with heat-denatured lysozyme as described above.
- a subtilisin-A-containing solution was prepared (0.04 mg/ml of water without excipients. activity: 0.0012 Au/ml) in such a manner as to have pH values between approximately 5.0 and 10.0.
- the osmolality value was then adjusted to 6. 133 and 264 mOsm/kg with NaCl for each of these solutions, each of the tonicity values being tested at pH 9.0.
- Lenses were then soaked in these solutions (five in each) for 2 hours at room temperature, rinsed, and analyzed for percentage residual protein deposit as described above. Results are given in Table II.
- subtilisin-A is an alkaline protease, having its greatest activity between pH 8 and 10, the greatest cleaning efficacy is observed in this pH range also.
- Example 3 Lenses with lysozyme protein deposits covering at least 98% of the lens surface were cut in half, one half being soaked in one enzyme solution and the other in another enzyme solution in the first comparison (pancreatin data); whole lenses were used in the other studies.
- the enzyme solutions were comprised of enzyme and excipients as indicated in Table III.
- Lenses were bufilcon-A sold by Barnes-Hind, Inc. under the name Hydrocurve II®. Results from each of the several formulations are listed in the following table. The abbreviation DI is used for deionized water. Table I I I
- a subtilisin enzyme tablet was dissolved in either 10ml of DI water or saline. Each enzyme tablet contained: 30mg N-acetylcysteine, 34mg sodiua carbonate, 7mg tartaric acid, 4mg polyethylene glycol 3350, 4mg subtillsin-A (subtilisin carlsberg from NOVO Industries of Denmark), and 50mg of lactose.
- Hydrocurve II lenses (55% H 2 O) from Barnes-Hind were coated with denatured lysozyme as described in Example 1. Three lenses were soaked in the 95mOsm/kg solution and four lenses in each of the other two solutions. Soaking time was 3.5 hours. Total percent surface cleaned was determined as per Example 1. the results are given in Table IV.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Detergent Compositions (AREA)
- Eyeglasses (AREA)
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT87900942T ATE83179T1 (en) | 1986-01-06 | 1987-01-05 | INCREASING ENZYMATIC ACTIVITY IN CLEANING CONTACT LENSES BY USING HYPOTONIC SOLUTIONS. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81651886A | 1986-01-06 | 1986-01-06 | |
US816518 | 1986-01-06 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP0252974A1 EP0252974A1 (en) | 1988-01-20 |
EP0252974A4 true EP0252974A4 (en) | 1988-05-03 |
EP0252974B1 EP0252974B1 (en) | 1992-12-09 |
Family
ID=25220860
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP87900942A Expired - Lifetime EP0252974B1 (en) | 1986-01-06 | 1987-01-05 | Enhancement of enzymatic activity in cleaning contact lenses by the use of hypotonic solutions |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0252974B1 (en) |
AT (1) | ATE83179T1 (en) |
AU (1) | AU6898887A (en) |
DE (1) | DE3782981T2 (en) |
HK (1) | HK113995A (en) |
WO (1) | WO1987004091A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5037647A (en) * | 1988-09-15 | 1991-08-06 | Alcon Laboratories, Inc. | Aqueous antimicrobial opthalmic solutions comprised of quaternary ammonium compound, citric acid, citrate and sodium chloride |
CA2009118C (en) * | 1989-02-21 | 1996-02-27 | Mary F. Mowrey-Mckee | Method and composition for cleaning and disinfecting contact lenses |
US5783532A (en) * | 1993-06-17 | 1998-07-21 | Allergan | Enzyme compositions and methods for contact lens cleaning |
IL109705A (en) * | 1993-06-17 | 1998-07-15 | Allergan Inc | Enzyme compositions and methods for contact lens cleaning |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2088581A (en) * | 1980-10-02 | 1982-06-09 | Smith & Nephew Associated Cie | Papain and lactose containing tablet for cleaning contact lenses |
GB2117534A (en) * | 1982-03-31 | 1983-10-12 | Smith & Nephew Ass | Papain containing tablet for cleaning contact lenses |
EP0219220A1 (en) * | 1985-09-09 | 1987-04-22 | Allergan, Inc | Method and composition for the simultaneous cleaning and disinfecting of contact lenses |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3240709A (en) * | 1962-05-16 | 1966-03-15 | Burton Parsons Chemicals Inc | Method of cleansing contact lenses |
US3910296A (en) * | 1973-04-20 | 1975-10-07 | Allergan Pharma | Method of removing proteinaceous deposits from contact lenses |
US4048122A (en) * | 1976-01-23 | 1977-09-13 | Barnes-Hind Pharmaceuticals, Inc. | Cleaning agents for contact lenses |
JPS54140553A (en) * | 1978-04-24 | 1979-10-31 | Senju Pharma Co | Contact lens washing liquid |
US4263054A (en) * | 1979-02-23 | 1981-04-21 | George D. Weaver | Contact lens cleaning and rinsing method |
US4521254A (en) * | 1981-02-09 | 1985-06-04 | Anderson Ronald L | Cleaning contact lenses with solution of bromelain and carboxypeptidase |
US4626292A (en) * | 1982-06-01 | 1986-12-02 | Sherman Laboratories, Inc. | Soft contact lens wetting and preservation method |
US4613380A (en) * | 1985-04-01 | 1986-09-23 | Dow Corning Corporation | Method for removing lipid deposits from contact lenses |
-
1987
- 1987-01-05 DE DE8787900942T patent/DE3782981T2/en not_active Expired - Fee Related
- 1987-01-05 AT AT87900942T patent/ATE83179T1/en active
- 1987-01-05 AU AU68988/87A patent/AU6898887A/en not_active Abandoned
- 1987-01-05 WO PCT/US1987/000045 patent/WO1987004091A1/en active IP Right Grant
- 1987-01-05 EP EP87900942A patent/EP0252974B1/en not_active Expired - Lifetime
-
1995
- 1995-07-13 HK HK113995A patent/HK113995A/en not_active IP Right Cessation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2088581A (en) * | 1980-10-02 | 1982-06-09 | Smith & Nephew Associated Cie | Papain and lactose containing tablet for cleaning contact lenses |
GB2117534A (en) * | 1982-03-31 | 1983-10-12 | Smith & Nephew Ass | Papain containing tablet for cleaning contact lenses |
EP0219220A1 (en) * | 1985-09-09 | 1987-04-22 | Allergan, Inc | Method and composition for the simultaneous cleaning and disinfecting of contact lenses |
Non-Patent Citations (1)
Title |
---|
See also references of WO8704091A1 * |
Also Published As
Publication number | Publication date |
---|---|
DE3782981T2 (en) | 1993-04-08 |
ATE83179T1 (en) | 1992-12-15 |
DE3782981D1 (en) | 1993-01-21 |
EP0252974B1 (en) | 1992-12-09 |
WO1987004091A1 (en) | 1987-07-16 |
AU6898887A (en) | 1987-07-28 |
EP0252974A1 (en) | 1988-01-20 |
HK113995A (en) | 1995-07-21 |
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