Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
  • A61K35/66—Microorganisms or materials therefrom
  • A61K35/74—Bacteria
  • A61K35/741—Probiotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
  • A61K36/06—Fungi, e.g. yeasts
  • A61K36/062—Ascomycota
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
  • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
  • A61K8/9728—Fungi, e.g. yeasts
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor
  • C12N1/145—Fungal isolates
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor
  • C12N1/16—Yeasts; Culture media therefor
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
  • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/08—Anti-ageing preparations
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
  • C12R2001/00—Microorganisms ; Processes using microorganisms
  • C12R2001/645—Fungi ; Processes using fungi
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S435/00—Chemistry: molecular biology and microbiology
  • Y10S435/8215—Microorganisms
  • Y10S435/911—Microorganisms using fungi

Definitions

• the present invention relates to a biological tricholon (Trich0sp0r0n).
• a culture solution of a strain of Ricshama (Seiken Bacteria No. ⁇ 2 /) is sterilized, and a sterile supernatant obtained by super-elimination, or the supernatant is concentrated by a conventional method.
• Dermatological medicament consisting of the concentrated concentrate or the concentrated or dried product itself, or a carrier containing such a medicament as an active ingredient, such as a conventional cream or ointment base for skin, etc.
• the present invention relates to a dermatological medical agent contained in
• the microorganism Trichosporum dynamatum strain used in the present invention is considered to belong to yeast, but may be classified as an incomplete bacterium. It can be identified as a bacterium belonging to the genus or the genus Endomy copsis, based on the academic views of technical researchers in the field. As shown in the attached figure (photograph), the fermented product, that is, the culture broth, as well as being different in that it has segmented spores and pseudohyphae which are different in the shape of a zigzag as shown in the attached figure (picture) The supernatant is unique in that it has a remarkable medical effect on human skin by itself.
• the substance of the active substance exhibiting a medical effect is not known, it is not possible to use a sterile supernatant of a culture solution and a concentrated or dried product of the substance alone, preferably in combination with an appropriate carrier.
• the fermented liquid has a weak acidity (pH..r.) And is harmless to the skin. 1) It promotes the protective action of the skin, especially the control of bacteria inherent in fat film.
• Trichosbo porphyrinum sclera strain (Seiken Bacteria No. ⁇ 2 /) used in the present invention are as follows! ) Is:
• Soluble starch So lub le Star ch
• This bacterium has remarkable properties in producing neutral odor components.
• the neutral odor components are ethanol, ethyl acetate, and isoprohy.
• inorganic nitrogen sources ammonium sulfate, ammonium nitrate, and salted ammonium; as organic nitrogen sources, yeast extract, casamino acid, Suitable for peptonka] 9
• aminoacids such as rosin, isocyanin isocyanin, and phenylalanine is remarkable;
• carbon source glucose, galactose, and carbon
• FIG. 9 is a photomicrograph showing the epiphytic state of segmental saturate mycelium cells under aerobic conditions (X7S). The segmented spores do not settle on the conidiophores and are derived from the septum of the hyphal cells into lateral laterals, and are chained out.
• Fig. 2 is a magnified micrograph of the sample.
• Figure J is a micrograph showing the cells and their budding morphology under anaerobic conditions (X
• a conventional yeast culture medium can be used to produce a dermatological medicament using the Trichos von borax spore strain.
• a medium having the following composition that is, gnorecose 0.3 ° h (/ V)-, skim milk.w / V), yeast ex. w Z v) in a solid or liquid medium at pH 0.0 to 0 and shaking or static at 2 to J ° C for about o2 hours.
• the inoculated culture is used as the inoculum, and is allowed to stand or shake cultured in the same medium for 2 to J days.
• the culture solution is removed in a conventional manner, for example, by filtration through a sample filter, and the sterile supernatant is concentrated, for example, under reduced pressure. .
• the concentrate is subjected to a sterilization step by a conventional method, and stored at room temperature or refrigerated.
• the aseptically filtered product of the culture supernatant of this strain contains small amounts of amino acids, carbohydrates, and fatty acids.
• the total nitrogen content is 0.00-0.0 ° h
• the total phosphorus content is 0.00-0.0 and 01.
• One example had an analysis value as shown in Table 2).
• Arsenide (As AS20 3) containing (as Pb) detected (detection limit 0. / ppm) Heavy metals Not detected (detection limit / ppm) without total mercury detection (detection limit / ppm.) - Power de Mi ⁇ No detection (detection limit./ ppm)
• the sterile supernatant can be directly used for the manufacture of a dermatological agent without concentrating, it is convenient for storage and other reasons to concentrate as described above. It is. Also, a concentrated or dried product having a concentration of twice or more is used. These concentrates, concentrates or dry matter can be used as dermatological agents themselves, or they can be mixed with creams, ointment bases and other carriers.
• the medical agent for skin of the invention is obtained. -VJ K t CMFI
• the sterile concentrate for use in the present invention is a this Ru binding ⁇ harm der of ⁇ test that by the Niwatorihaite scan: it is proof, also human c 0 pitch te be sampled
• the substance was shown to be harmless to the skin by skin irritation due to discontinuous human skin and to be non-M violent in 5 rabbit eye irritation tests.
• it was found that it contained antibiotics.
• the human body ',. Test The product of the present invention (double concentration #J) Fishing 2 ⁇ square. Apply it to Tsuchi Kizuna and apply it to a woman 2 years old or older (a patient with any skin condition who is visiting a dermatological specialist hospital) on the medial left upper arm and back paravertebra. At the time of application, the adhesive bond was removed. J was evaluated at J minutes after the removal. As a result, there were many cases showing positive results in the inner left upper arm and the paravertebral part of the back.
• OVP OVP! The name was applied to an outpatient of a dermatologist hospital with some dermatological symptoms, and the name was applied to the right face of a female employee at the hospital for a minute. Continued. All the subjects were unresponsive], and none of them showed abnormalities such as erythema.
• Rabbit eye irritation test This product (double concentrated solution) was used for the right eye of a white breed rabbit head, which was previously confirmed to have no abnormality in the eyes, and a blank test solution was used for the left eye. The eye was instilled one by one, and the eyes were examined using a slit lamp immediately after instillation, three, three, and seven hours later. As a result, the cornea, iris and conjunctiva of both eyes were congested in both observations. No other abnormalities were observed.
• Treatment test for stains Excludes breathers, gynecological patients, and pregnant women with liver disease, facial skin stains, and facial cleansing for women / names who are visiting a dermatology hospital.
• the pores are then opened with ozone and steam, and the product of the present invention (a twice-concentrated solution of the sterile supernatant) is applied to a gauze and brought into close contact with the spot formation site.
• the product was covered with a Saran wrap and irradiated with infrared light for / S ⁇ minutes to allow the product to penetrate the skin. This process was continued / weekly / once for power months.
• the present invention which is effective in treating rough skin because it is easy to treat small rough skin as the rough skin progresses, is a treatment test for c- contact dermatitis that achieves the prevention of small rough skin. : The day after the use was changed from Company A's shadow to Company B's shadow, redness and pruritus occurred on the eyelids on both sides of the face from the next day, and contact dermatitis was diagnosed. Done
• OMPI A dermatologist against a 2 I-year-old girl]?
• the gauze containing the product of the present invention ( ⁇ double binding) was applied to the affected area.
• only the product of the present invention was used without using a steroid preparation, an anti-inflammatory agent or an antihistamine agent.
• Treatment test for one burn Burns due to boiling water with palmar-sized redness swelling and partial vesicle formation on the left front knee outside the skin. A 7-year-old woman had skin injuries about / hour after being injured by the boiling water. Visited a specialist. On the other hand, the affected area was immediately cooled with tap water (running water), and then a gauze containing the product of the present invention (double concentrate) was placed on the breathing area, covered with oil paper, and bandaged. ⁇ Hours later, erythematous swelling remained in the affected area, but the vesicles had disappeared and there was no pain. A similar treatment was performed on a male J-year-old who had burned chicken-sized red swelling on the back of his right foot with boiling water. Two hours later, some redness remained, but there was no sleep, no pain, and significant erection was observed.
• composition medium 0 ⁇ was sterilized by heating in a /// volume aeration-stirring fermentation tank and allowed to cool.
• OVFI Inoculate the mixture as a seed culture and incubate for about 7 hours at 2 S ° C with aeration and agitation. After the fermentation is completed, the culture broth is supplied to the internal and external food processing equipment Co., Ltd., MP 293- /
• the iF filter obtained by removing solid particles and cells was removed by a membrane filter (membrane filter: Toyo ⁇ ⁇ TM-20 ⁇ ).
• the solution is concentrated to 200 ⁇ by the reverse immersion method (R0- ⁇ / type torsion, manufactured by K-Ning Ring Co., Ltd.).
• the resulting concentrate 3 ⁇ 43 ⁇ 4.
• a rate-type heat exchanger manufactured by Iwai Machinery Co., Ltd.
• the solution is aseptically filled in a glass container, cooled (s ° C) and stored.
• a powdered product obtained by freeze-drying from the sterilized syrup obtained in Example 1 was mixed with a sterile ointment base material to produce a dermatological medical agent.
• Another production example of a dermatological agent is as follows. Immediately, steric acid / ⁇ , miro 7 ⁇ , self-emulsifying
• the dermatological agent of the present invention is derived from natural substances and is proven to be harmful, it can be used not only for treatment of skin disorders but also for treatment of mucosal disorders, and protects epithelium from pollution. It is expected to be widely used to maintain good health.

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• Life Sciences & Earth Sciences (AREA)
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• Preparation Of Compounds By Using Micro-Organisms (AREA)

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• 1979
  • 1979-09-01 JP JP11229479A patent/JPS5636419A/ja active Granted
• 1980
  • 1980-08-26 FI FI802690A patent/FI68079C/fi not_active IP Right Cessation
  • 1980-08-26 PH PH24487A patent/PH22011A/en unknown
  • 1980-08-29 CA CA000359366A patent/CA1176586A/en not_active Expired
  • 1980-08-29 CS CS805920A patent/CS247055B2/cs unknown
  • 1980-08-29 ZA ZA00805361A patent/ZA805361B/xx unknown
  • 1980-08-29 ES ES494636A patent/ES494636A0/es active Granted
  • 1980-08-30 IN IN994/CAL/80A patent/IN153038B/en unknown
  • 1980-09-01 DE DE8080105199T patent/DE3069020D1/de not_active Expired
  • 1980-09-01 AT AT80105199T patent/ATE9100T1/de not_active Application Discontinuation
  • 1980-09-01 WO PCT/JP1980/000203 patent/WO1981000723A1/ja not_active Ceased
  • 1980-09-01 UA UA3278848A patent/UA6318A1/uk unknown
  • 1980-09-01 US US06/261,167 patent/US4503041A/en not_active Expired - Fee Related
  • 1980-09-01 EP EP80105199A patent/EP0024738B1/en not_active Expired
  • 1980-09-01 HU HU801153A patent/HU186767B/hu unknown
  • 1980-09-01 AR AR282377A patent/AR228434A1/es active
  • 1980-09-01 DE DE198080105199T patent/DE24738T1/de active Pending
  • 1980-09-02 MX MX809004U patent/MX6444E/es unknown
• 1981
  • 1981-04-30 SU SU813278848A patent/SU1327791A3/ru active
  • 1981-05-01 DK DK194981A patent/DK157035C/da not_active IP Right Cessation
• 1984
  • 1984-02-09 US US06/578,308 patent/US4554161A/en not_active Expired - Lifetime

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