UY36522A - Ultracentrifugación analítica para la caracterización de partículas virales recombinantes - Google Patents

Ultracentrifugación analítica para la caracterización de partículas virales recombinantes

Info

Publication number
UY36522A
UY36522A UY0001036522A UY36522A UY36522A UY 36522 A UY36522 A UY 36522A UY 0001036522 A UY0001036522 A UY 0001036522A UY 36522 A UY36522 A UY 36522A UY 36522 A UY36522 A UY 36522A
Authority
UY
Uruguay
Prior art keywords
recombinant
recombinant viral
particles
viral particles
analytical ultracentrifugation
Prior art date
Application number
UY0001036522A
Other languages
English (en)
Inventor
Catherine O Riordan
Burnham Brenda
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55305080&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=UY36522(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of UY36522A publication Critical patent/UY36522A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
    • G01N2015/045—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates by optical analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporcionan aquí métodos para caracterizar preparaciones de partículas virales recombinantes usando ultracentrifugación analítica. Las partículas virales recombinantes incluyen partículas virales adeno-asociadas recombinantes, partículas adenovirales recombinantes, partículas lentivirales recombinantes y partículas de virus herpes simplex recombinantes. Se pueden identificar y cuantificar especies variantes de partículas virales recombinantes que incluyen cápsides vacías y partículas virales recombinantes con genomas variantes (v.g., genomas truncados, agregados, recombinantes). Los métodos se pueden usar para caracterizar preparaciones de partículas virales recombinantes independientemente de la secuencia del genoma viral recombinante o el serotipo de la cápside viral recombinante.
UY0001036522A 2015-01-20 2016-01-20 Ultracentrifugación analítica para la caracterización de partículas virales recombinantes UY36522A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562105714P 2015-01-20 2015-01-20

Publications (1)

Publication Number Publication Date
UY36522A true UY36522A (es) 2016-08-31

Family

ID=55305080

Family Applications (1)

Application Number Title Priority Date Filing Date
UY0001036522A UY36522A (es) 2015-01-20 2016-01-20 Ultracentrifugación analítica para la caracterización de partículas virales recombinantes

Country Status (13)

Country Link
US (5) US10429288B2 (es)
EP (1) EP3247793A1 (es)
JP (4) JP2018505695A (es)
KR (1) KR20170104594A (es)
CN (1) CN107646052A (es)
AR (1) AR105412A1 (es)
AU (1) AU2016209471B2 (es)
CA (1) CA2974378A1 (es)
EA (1) EA201791630A1 (es)
IL (4) IL310375A (es)
TW (1) TWI745285B (es)
UY (1) UY36522A (es)
WO (1) WO2016118520A1 (es)

Families Citing this family (11)

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KR20170104594A (ko) 2015-01-20 2017-09-15 젠자임 코포레이션 재조합 바이러스 입자의 특성규명을 위한 분석용 초원심분리
WO2019183605A1 (en) * 2018-03-23 2019-09-26 University Of Massachusetts Gene therapeutics for treating bone disorders
EP3696534A1 (en) * 2019-02-14 2020-08-19 SmartDyeLivery GmbH Method for determining physicochemical properties of nanoscale systems (nss)
TW202142692A (zh) * 2020-01-29 2021-11-16 美商航海家醫療公司 用於生產aav顆粒之方法及系統
GB202004254D0 (en) 2020-03-24 2020-05-06 Puridify Ltd Characterization of gene therapy vectors
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
US20240159718A1 (en) 2021-03-22 2024-05-16 Genzyme Corporation Size exclusion chromatography analysis of empty and full aav capsids
WO2022224697A1 (ja) 2021-04-19 2022-10-27 国立大学法人大阪大学 分子送達用粒子を特徴づけるための方法
TWI787809B (zh) * 2021-05-07 2022-12-21 台達電子工業股份有限公司 資料預篩方法及其資料預篩裝置
WO2024025787A1 (en) * 2022-07-26 2024-02-01 Brammer Bio, Llc Analytical ultracentrifugation methods
WO2024079655A1 (en) 2022-10-11 2024-04-18 Meiragtx Uk Ii Limited Chromatography methods for purification of aav capsids

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WO1998035062A1 (en) * 1997-02-07 1998-08-13 Lingappa Jaisri R Multistep, atp-dependent cell-free system for the assembly of human immunodeficiency virus capsids
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EP2281877A3 (en) 2003-05-21 2011-06-01 Genzyme Corporation Methods for producing preparations of recombinant AAV virions substantially free of empty capsids
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US8663624B2 (en) 2010-10-06 2014-03-04 The Regents Of The University Of California Adeno-associated virus virions with variant capsid and methods of use thereof
MX353930B (es) 2011-02-17 2018-02-02 Univ Pennsylvania Composiciones y metodos para alterar la especificidad tisular y mejorar la transferencia de genes mediada por vector viral adeno-asociado 9.
FR3002237B1 (fr) 2013-02-15 2017-12-15 Genethon Methodes pour la production de particules virales aav double brin
EP3230441A4 (en) * 2014-12-12 2018-10-03 Voyager Therapeutics, Inc. Compositions and methods for the production of scaav
KR20170104594A (ko) 2015-01-20 2017-09-15 젠자임 코포레이션 재조합 바이러스 입자의 특성규명을 위한 분석용 초원심분리

Also Published As

Publication number Publication date
EA201791630A1 (ru) 2018-04-30
IL253542A0 (en) 2017-09-28
KR20170104594A (ko) 2017-09-15
IL253542B (en) 2020-05-31
TW201639958A (zh) 2016-11-16
CN107646052A (zh) 2018-01-30
IL274137B (en) 2022-10-01
IL296391B1 (en) 2024-02-01
JP2021000110A (ja) 2021-01-07
AU2016209471A1 (en) 2017-09-07
IL274137A (en) 2020-06-30
AU2016209471B2 (en) 2022-03-31
US20180180525A1 (en) 2018-06-28
TWI745285B (zh) 2021-11-11
CA2974378A1 (en) 2016-07-28
IL310375A (en) 2024-03-01
AR105412A1 (es) 2017-10-04
US12031894B2 (en) 2024-07-09
US20230288308A1 (en) 2023-09-14
US10429288B2 (en) 2019-10-01
IL296391A (en) 2022-11-01
IL274137B2 (en) 2023-02-01
JP2024113102A (ja) 2024-08-21
JP2022095863A (ja) 2022-06-28
JP2018505695A (ja) 2018-03-01
US12085494B2 (en) 2024-09-10
US20240077402A1 (en) 2024-03-07
WO2016118520A1 (en) 2016-07-28
IL296391B2 (en) 2024-06-01
EP3247793A1 (en) 2017-11-29
US12013326B2 (en) 2024-06-18
US20240085301A1 (en) 2024-03-14
US20200225139A1 (en) 2020-07-16
US11639887B2 (en) 2023-05-02

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Effective date: 20221027