BR112018071180A2 - métodos de reforço potência biológica de baculovirus system-produzido vírus adeno-associado recombinante - Google Patents

métodos de reforço potência biológica de baculovirus system-produzido vírus adeno-associado recombinante

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Publication number
BR112018071180A2
BR112018071180A2 BR112018071180-2A BR112018071180A BR112018071180A2 BR 112018071180 A2 BR112018071180 A2 BR 112018071180A2 BR 112018071180 A BR112018071180 A BR 112018071180A BR 112018071180 A2 BR112018071180 A2 BR 112018071180A2
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BR
Brazil
Prior art keywords
methods
associated virus
recombinant adeno
biological potency
enhancing biological
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BR112018071180-2A
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English (en)
Inventor
Zolotukhin Sergei
Kondratov Oleksandr
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University Of Florida Research Foundation, Incorporated
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Application filed by University Of Florida Research Foundation, Incorporated filed Critical University Of Florida Research Foundation, Incorporated
Publication of BR112018071180A2 publication Critical patent/BR112018071180A2/pt

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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14051—Methods of production or purification of viral material
    • C12N2710/14052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14144—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
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    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/50—Vectors for producing vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/10—Vectors comprising a special translation-regulating system regulates levels of translation
    • C12N2840/105—Vectors comprising a special translation-regulating system regulates levels of translation enhancing translation

Abstract

a presente invenção fornece composições e métodos úteis para a produção de aav recombinante (raav) em células de inseto. em algumas concretizações, métodos e composições incluem o uso de sequências kozak modificadas para expressar proteínas de aav vp1 em quantidades que são úteis para a produção de partículas infecciosas de raav.
BR112018071180-2A 2016-04-16 2017-04-16 métodos de reforço potência biológica de baculovirus system-produzido vírus adeno-associado recombinante BR112018071180A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662323684P 2016-04-16 2016-04-16
US62/323,684 2016-04-16
PCT/US2017/027832 WO2017181162A1 (en) 2016-04-16 2017-04-16 Methods of enhancing biological potency of baculovirus system-produced recombinant adeno-associated virus

Publications (1)

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BR112018071180A2 true BR112018071180A2 (pt) 2019-02-12

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BR112018071180-2A BR112018071180A2 (pt) 2016-04-16 2017-04-16 métodos de reforço potência biológica de baculovirus system-produzido vírus adeno-associado recombinante

Country Status (12)

Country Link
US (1) US11384364B2 (pt)
EP (1) EP3442985A4 (pt)
JP (1) JP6975980B2 (pt)
KR (2) KR102598289B1 (pt)
CN (1) CN109311932B (pt)
AU (1) AU2017248840B2 (pt)
BR (1) BR112018071180A2 (pt)
CA (1) CA3021080A1 (pt)
IL (1) IL262353B2 (pt)
MX (1) MX2018012663A (pt)
RU (1) RU2762948C2 (pt)
WO (1) WO2017181162A1 (pt)

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CN109311932A (zh) 2019-02-05
JP6975980B2 (ja) 2021-12-01
KR20230156167A (ko) 2023-11-13
IL262353B2 (en) 2023-06-01
RU2018138778A3 (pt) 2021-02-16
CN109311932B (zh) 2022-05-03
EP3442985A4 (en) 2019-12-18
US11384364B2 (en) 2022-07-12
MX2018012663A (es) 2019-07-08
RU2018138778A (ru) 2020-05-18
KR20180134384A (ko) 2018-12-18
IL262353A (en) 2018-11-29
JP2019513403A (ja) 2019-05-30
US20210222194A1 (en) 2021-07-22
KR102598289B1 (ko) 2023-11-06
WO2017181162A1 (en) 2017-10-19
RU2762948C2 (ru) 2021-12-24
CA3021080A1 (en) 2017-10-19
AU2017248840A1 (en) 2018-11-08
EP3442985A1 (en) 2019-02-20
AU2017248840B2 (en) 2022-07-28

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